海洋贝类活性肽的研究现状和应用前景
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摘要
中国近海有丰富的贝类资源,贝肉以及其副产物主要用于冷鲜、干制品以及风味酱料加工,大部分下脚料加工利用率低,被丢掉后在一定程度上也造成了环境污染。酶法制备活性肽,研究其结构与功能活性的关系和活性肽的应用是当前国内外研究的热点。综述国内外相关的制备纯化和构效研究的进展,分析和探讨贝类活性肽在生产中存在的一些问题,并阐明海洋贝类活性肽在食品工业、医药、化妆品和饲料等领域中的潜在应用价值及前景,为进一步高值化开发利用海洋贝类提供参考。
There is a rich resource of shellfishes in coastal waters in China.The shellfish meat and its by-products are mainly used to chilled processing,dried and some flavor sauce products.However,a mass of waste by-products are neither beneficial to the environment nor in high value processing,in a certain extent.In view of this,more and more domestic and foreign researches come to pay attention to the enzymatic preparation and purification of bioactive peptide from shellfish,the exploration of its structure-activity relationship and its application of active peptide.The status quo and the problems exist in the present were reviewed and summarized in this paper.Given that,shellfish active peptides had potential applications in medicine,cosmetics and food industry,etc.
There is a rich resource of shellfishes in coastal waters in China.The shellfish meat and its by-products are mainly used to chilled processing,dried and some flavor sauce products.However,a mass of waste by-products are neither beneficial to the environment nor in high value processing,in a certain extent.In view of this,more and more domestic and foreign researches come to pay attention to the enzymatic preparation and purification of bioactive peptide from shellfish,the exploration of its structure-activity relationship and its application of active peptide.The status quo and the problems exist in the present were reviewed and summarized in this paper.Given that,shellfish active peptides had potential applications in medicine,cosmetics and food industry,etc.
引文
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[3]吴燕燕,田倩,尚军,等.合浦珠母贝抗氧化肽的性质及应用研究[J].食品工业科技,2011,32(11):123-126,130.
[4]张泽,赵前程,程继龙,等.长牡蛎蛋白双酶水解工艺及其产物的抗氧化活性[J].湖南农业科学,2012,52(11):2315-2317.
[5]林娈,陈丽娇.文蛤肉复合酶分步酶解工艺的研究[J].食品科学,2009,30(9):158-162.
[6]张艳萍,戴志远,张虹.紫贻贝酶解物中降血压肽的超滤分离[J].食品与发酵工业,2010,36(9):46-51.
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[10]赵听友,曹瑛,代先东,等.新型桶形芋螺毒素BtIIIB的分离纯化、氨基酸序列测定及二硫键定位研究[J].化学学报,2005,63(2):163-168.
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[13]阎欲晓,粟桂娇,李小梅,等.文蛤蛋白抗氧化活性肽的研究[J].食品工业科技,2007,28(12):121-123.
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[15]Rajapakse N,Mendis E,Byun H G,et al.Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical mediated oxidative systems[J].J Nutr Biochem,2005,(16):562-569.
[16]Rajapakse Niranjan,Mendis Eresha,Jung Won-Wyo,et al.Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties[J].Food Res Earch International,2005,38(2):175-182.
[17]王日昕,刘梅,廖智,等.厚壳贻贝抗菌肽Mytilin的初步鉴定[J].水产学报,2010,34(1):153-158.
[18]杨林,范美华,刘雪珠,等.两种合成抗菌肽的结构及抗菌作用机理[J].生物工程学报,2011,27(11):1564-1573.
[19]刘娜.中国南海新型芋螺毒素的化学合成、活性测定及表达条件初探[D].青岛:山东科技大学,2011.
[20]郑炯,邓惠玲,林茂,等.ACE抑制肽的酶法制备及其构效关系的研究进展[J].食品工业科技,2012,33(15):418-422,427.
[21]魏照征,田健,伍宁丰,等.抗菌肽构效关系及其表达策略研究进展[J].生物技术通报,2011(2):7-11.
[22]李影,巨芳,张艳华,等.生物活性多肽的分离纯化及其发展趋势[J].江苏调味副食品,2009,26(3):33-36.
[23]段涛,叶磊.降血压肽的研究及应用前景[J].江苏食品与发酵,2008(4):14-17.
[24]陈汉源,丛笑倩,张昂,等.文蛤抽提物的抗癌研究[J].肿瘤防治研究,1980,11(4):3-7.
[25]蔡冰娜,吴园涛,孙恢礼.牡蛎肽肠内营养制剂对小鼠肠道功能作用的研究[J].中国食物与营养,2010,(5):64-67.
[26]宋宏霞,曾名勇.紫贻贝抗菌肽的研究[D].青岛:中国海洋大学,2007:57-70.
[27]孙恢礼,陈华,陈忻,等.一种含有海洋贝类活性肽的化妆品及其制备方法和应用:中国,201110079404.0[P].2011-09-21[2012-08-28].http://www.aptchina.com/faming/6643790/download.html.
[28]孙恢礼,陈华,陈忻,等.一种用于细致毛孔的海洋生物功能化妆品:中国,201110106633.7[P].2011-09-14[2012-08-28].http://www.aptchina.com/faming/6534792/download.html.
[2]吴燕燕,尚军,李来好,等.合浦珠母贝肉短肽的分离及其抗氧化活性研究[J].食品工业科技,2012,33(7):123-126.
[3]吴燕燕,田倩,尚军,等.合浦珠母贝抗氧化肽的性质及应用研究[J].食品工业科技,2011,32(11):123-126,130.
[4]张泽,赵前程,程继龙,等.长牡蛎蛋白双酶水解工艺及其产物的抗氧化活性[J].湖南农业科学,2012,52(11):2315-2317.
[5]林娈,陈丽娇.文蛤肉复合酶分步酶解工艺的研究[J].食品科学,2009,30(9):158-162.
[6]张艳萍,戴志远,张虹.紫贻贝酶解物中降血压肽的超滤分离[J].食品与发酵工业,2010,36(9):46-51.
[7]黄海,尹玲.利用贝类废弃物制造水解动物蛋白[J].日照职业技术学院学报,2006,1(3):19-21.
[8]杨永芳,闫海强,丁国芳,等.贻贝水解物抗肿瘤活性肽的分离纯化[J].中国药科大学学报,2011,(3):272-275.
[9]Wang Jiapei,Hu Jianen,Cui Jinzhe,et al.Purification and Identification of a ACE Inhibitory Peptide from Oyster Proteins Hydrolysate and the Antihypertensive Effect of Hydrolysate in Spontaneously Hypertensive Rats[J].Food Chemistry,2008,111(2):302-308.
[10]赵听友,曹瑛,代先东,等.新型桶形芋螺毒素BtIIIB的分离纯化、氨基酸序列测定及二硫键定位研究[J].化学学报,2005,63(2):163-168.
[11]Pedro Lozano,Didier Combes,Joseluis Ibovra,et al.Food protein nutrient improvement by protease at reduced water activity[J].Journal of Food Science,1994,54(4):876-880.
[12]汪秋宽,刘红丹,徐坚,等.牡蛎酶解液的抗氧化活性[J].中国水产科学,2007,14(2):296-301.
[13]阎欲晓,粟桂娇,李小梅,等.文蛤蛋白抗氧化活性肽的研究[J].食品工业科技,2007,28(12):121-123.
[14]Won K J,Niranjan R,Se K K.Antioxidative activity of a low molecular weight peptide derived from the sauce of fermented blue mussel,Mytilus edulis[J].Eur Food Res Technol,2005,220:535-539.
[15]Rajapakse N,Mendis E,Byun H G,et al.Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical mediated oxidative systems[J].J Nutr Biochem,2005,(16):562-569.
[16]Rajapakse Niranjan,Mendis Eresha,Jung Won-Wyo,et al.Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties[J].Food Res Earch International,2005,38(2):175-182.
[17]王日昕,刘梅,廖智,等.厚壳贻贝抗菌肽Mytilin的初步鉴定[J].水产学报,2010,34(1):153-158.
[18]杨林,范美华,刘雪珠,等.两种合成抗菌肽的结构及抗菌作用机理[J].生物工程学报,2011,27(11):1564-1573.
[19]刘娜.中国南海新型芋螺毒素的化学合成、活性测定及表达条件初探[D].青岛:山东科技大学,2011.
[20]郑炯,邓惠玲,林茂,等.ACE抑制肽的酶法制备及其构效关系的研究进展[J].食品工业科技,2012,33(15):418-422,427.
[21]魏照征,田健,伍宁丰,等.抗菌肽构效关系及其表达策略研究进展[J].生物技术通报,2011(2):7-11.
[22]李影,巨芳,张艳华,等.生物活性多肽的分离纯化及其发展趋势[J].江苏调味副食品,2009,26(3):33-36.
[23]段涛,叶磊.降血压肽的研究及应用前景[J].江苏食品与发酵,2008(4):14-17.
[24]陈汉源,丛笑倩,张昂,等.文蛤抽提物的抗癌研究[J].肿瘤防治研究,1980,11(4):3-7.
[25]蔡冰娜,吴园涛,孙恢礼.牡蛎肽肠内营养制剂对小鼠肠道功能作用的研究[J].中国食物与营养,2010,(5):64-67.
[26]宋宏霞,曾名勇.紫贻贝抗菌肽的研究[D].青岛:中国海洋大学,2007:57-70.
[27]孙恢礼,陈华,陈忻,等.一种含有海洋贝类活性肽的化妆品及其制备方法和应用:中国,201110079404.0[P].2011-09-21[2012-08-28].http://www.aptchina.com/faming/6643790/download.html.
[28]孙恢礼,陈华,陈忻,等.一种用于细致毛孔的海洋生物功能化妆品:中国,201110106633.7[P].2011-09-14[2012-08-28].http://www.aptchina.com/faming/6534792/download.html.