对虾中隐蔽态T-2毒素残留对雄性小鼠生长发育的影响及其遗传毒性效应
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摘要
为探明对虾中隐蔽态T-2染毒对小鼠生长发育及遗传方面的毒性效应,采用定期递增剂量法进行20 d蓄积染毒实验,以对虾的虾头和肌肉匀浆液灌胃小鼠,连续7 d,记录小鼠器官指数、精子致畸率、微核率,分析对虾中T-2及隐蔽态残留物对小鼠的毒害作用。结果表明,低剂量染毒对虾对小鼠没有影响,高剂量组(12.2 mg/kg)染毒对虾引起小鼠增重率和器官系数下降,精子畸形率及微核率均显著高于对照组(P<0.05);随着染毒剂量的增加,精子畸形率及微核率呈上升趋势。染毒对虾中存在隐蔽态的T-2毒素,对小鼠有一定的遗传毒性,虾头中隐蔽态T-2毒素的含量高于肌肉中的,并与染毒剂量呈正相关。
Proven the toxic effect of masked T-2 toxin in Litopenaeus vannamei on growth and development and genetic of male mice. Mice, with head and muscle homogenates of the shrimps which were conducted the experiment of20 d accumulation toxicity with Does-periodic-increased methods through tube feeding for 7 d. Then recording the organ index, spermatozoa deformity and micronucleus of marrow cell were measured. Analysis of the toxic effects of T-2 and the hidden residues in the shrimp on mice. The results showed that low doses T-2 infected shrimp has no effect on mice. However, high dose T-2 infected shrimp caused decreasing of organ index. Sperm deformity rate and micronucleus rate were significantly higher than that of the control group(P<0.05); With the increase of the exposure dose, the abnormal rate of sperm and the rate of micronucleus increased. Research shows T-2 in feed could transformed into masked T-2 toxins(m T-2 s) by shrimp after ingesting, T-2 was exposed to a certain genetic toxicity, and the contents of the masked T-2 in the head were higher than that in the muscle; which was positively correlated with the exposure dose.
Proven the toxic effect of masked T-2 toxin in Litopenaeus vannamei on growth and development and genetic of male mice. Mice, with head and muscle homogenates of the shrimps which were conducted the experiment of20 d accumulation toxicity with Does-periodic-increased methods through tube feeding for 7 d. Then recording the organ index, spermatozoa deformity and micronucleus of marrow cell were measured. Analysis of the toxic effects of T-2 and the hidden residues in the shrimp on mice. The results showed that low doses T-2 infected shrimp has no effect on mice. However, high dose T-2 infected shrimp caused decreasing of organ index. Sperm deformity rate and micronucleus rate were significantly higher than that of the control group(P<0.05); With the increase of the exposure dose, the abnormal rate of sperm and the rate of micronucleus increased. Research shows T-2 in feed could transformed into masked T-2 toxins(m T-2 s) by shrimp after ingesting, T-2 was exposed to a certain genetic toxicity, and the contents of the masked T-2 in the head were higher than that in the muscle; which was positively correlated with the exposure dose.
引文
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[21]GB 15193.5-2003骨髓细胞微核试验.ICS 07.100.53[S].北京:中国标准出版社,2003.
[22]靳生,王冠颖,杜强,等.阿特拉津对雏鸡的急性毒性作用和骨髓微核率的影响[J].中国兽医杂志,2013,49(4):72-73.
[2]刘磊,张国巍,丁博,等.T-2毒素研究进展[J].吉林医学院学报,2013,4(34):115-119.
[3]PESTKA J J.Deoxynivalenol:mechanisms of action,human exposure,and toxicological relevance[J].Archives of Tox-icolog,2010,84(9):663-679.
[4]PESTKA J J,ZHOU H R,MOON Y,et al.Cellular and molecular mechanisms for immune modulation by deoxynivalenol and other trichothecenes:unraveling a paradox[J].Toxicol Lett,2004,153(1):16-73.
[5]AGRAWAL M,YADAV P,LOMASH V,et al.T-2 toxin induced skin inflammation and cutaneous injury in mice[J].Toxicology,2012,302(2/3):255-265.
[6]王雅玲,代喆,孙力军,等.T-2毒素对凡纳滨对虾的经口急性毒性效应研究[J].现代食品科技,2015,31(1):43-47.
[7]SANTACROCE M P,CONVERSANO M,CASALINO E,et al.Aflatoxins in aquatic species:metabolism,toxicity and perspectives[J].Rev Fish Biol Fisheries,2008,18:99-130.
[8]HOOFT J M,ELMOR H I,ENCARNACAO P,et al.Rainbow trout(Oncorhynchus mykiss)is extremely sensitive to the feed-borne Fusarium mycotoxin deoxynivalenol(DON)[J].Aquaculture,2011,311(1-4):224-232.
[9]BUNDIT O,KANGHAE H,PHROMKUNTHONG W,et al.Effects of mycotoxin T-2 and zearalenone on histopathological changes in black tiger shrimp(Penaeus monodon Fabricius)[J].Songklanakarin Journal of Science and Technology,2006,28(5):937-949.
[10]SUPAMATTAYA K,BUNDIT O,BOONYARAPATLIN M,et al.Effects of mycotoxins T-2 and Zearalenone on growth performance immuno-ohysiological parameters and histological changes in Black tiger shrimp(Penaeus monodon)and white shrimp(Litopenaeus vannamei)[J].XII International Symposium of Fish Nutrition&Feeding,2006,41:218-221.
[11]代喆.T-2毒素诱导凡纳滨对虾肌肉品质典型性状的变化规律[D].湛江:广东海洋大学,2013.
[12]邱妹.对虾中隐蔽态T-2毒素危害特征与免疫毒性分子标记识别[D].湛江:广东海洋大学,2015.
[13]PESTKA J J.Toxicological mechanisms and potential health effects of deoxynivalenol and nivalenol[J].World Mycotoxin J,2010,3(3):323-347.
[14]WENDA W,HUIREN Z,XIAO P,et al.Comparison of anorectic potencies of the trichothecenes T-2 toxin,HT-2 toxin and satratoxin G to the ipecac alkaloid emetine[J].Toxicology Reports,2015,2(1):238-251.
[15]WANG Z G,FENG J N,TONG Z.Human toxicosis caused by moldy rice contaminated with Fusarium and T-2toxin[J].Biomed Environ Sci,1993,6(1):65-70.
[16]张自广,石念进,王希春,等.中药复方多糖对小鼠免疫功能的影响[J].畜牧与兽医,2012,44(2):81-85.
[17]VILA B,JARADAT Z W,MARQUARDT R R,et al.Effect of T-2 toxin on in vivo lipid peroxidation and vitamin E status in mice[J].Food and Chemical Toxicology,2002,40(4):479-486.
[18]袁莉芸,袁慧,陈铁桥.低剂量T-2毒素对雏鸡免疫器官及血清生化指标的影响[J].中国家禽,2007,29(17):14-16.
[19]SHINYA S,NAOKI K,FUSAKO A,et al.Microarray analysis of T-2 toxin-induced liver,placenta and fetal liver lesions in pregnant rats[J].Experimental and Toxicologic Pathology,2005,57(1):15-28.
[20]欧仁福,魏星华,姜斌.磺胺氯吡嗪钠染毒小鼠精子畸形率及骨髓细胞微核的变化[J].毒理学杂志,2010,24(4):294-296.
[21]GB 15193.5-2003骨髓细胞微核试验.ICS 07.100.53[S].北京:中国标准出版社,2003.
[22]靳生,王冠颖,杜强,等.阿特拉津对雏鸡的急性毒性作用和骨髓微核率的影响[J].中国兽医杂志,2013,49(4):72-73.