摘要
菌种保存工作是医疗卫生、科学研究、实践教学中重要的基础性工作,选用教学实验室常用的6种保存菌种方法,对它们的优缺点进行对比。结果表明,瓷珠菌种保存法简单方便,保存快速,价格经济,保存时间长,占用空间小且适用范围广,实用性较强。瓷珠菌种保存方法有效地提高了菌种保存工作的效率,目前已经被各临床及教学实验室广泛应用。相信在不远的将来一定会取代传统的菌种保存方法,得到进一步普及。
The preservation of bacteria species is an important basic work in medical and health care,scientific research and practical teaching, six methods of preservation of bacteria were used in teachinglaboratory and their advantages and disadvantages were compared. The result showed that the preserva-tion method of porcelain beads is simple and convenient, the preservation is quick, the price is eco-nomical, the preservation time is long, the space occupied is small, the scope of application is wide,and the practicability is strong. Which also effectively improves the efficiency, the method has been ac-cepted and applied in clinical and teaching laboratories. It is believed that in the near future, it will re-place the traditional methods of species preservation and be further popularized.
引文
[1]杨旭,刘晓莉,吴防,等.两种简便保存菌种的方法比较[J].检验医学与临床,2008,5(20):1219-1220.
[2]杨芳,刘利兵,刘芳娥.常用教学菌种保存方法[J].第四军医大学学报,2004,25(9):814.
[3]吴茂红,吴时耕.菌种的保存方法介绍[J].江西医学检验,2004,22(6):545-546.
[4]曹美林,李佳,邓慧,等.滤纸片低温冷冻法保存微生物菌株的临床应用[J].实验与检验医学,2015,33(3):279-281.
[5]郭淑清,田峰.菌种的甘油冷冻保存法[J].山西医科大学学报,2000,31(4):382.
[6]张爱梅,郭大城,王建丽,等.国内菌种保藏材料及保藏方法研究现状[J].河南预防医学杂志,2011,22(6):405-407,412.
[7]尹洪萍,蔡玲斐.一种脱纤维血冷冻菌种保存法[J].杭州医学高等专科学校学报,2003,24(1):25.
[8]陈晓燕,林光.微生物实验菌种保存方式探讨[J].临床合理用药,2014,7(10):129-130.