新型绿色低共熔溶剂用于金钱草总黄酮的提取研究
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摘要
目的 首次利用新型绿色低共熔溶剂结合超声波辅助法对金钱草总黄酮的提取工艺条件进行探讨。方法 以金钱草总黄酮的提取率为指标,在单因素试验的基础上,采用星点设计-响应面法,考察低共熔溶剂含水量、提取温度、液料比3个显著因素对提取工艺的影响。结果 最佳提取工艺条件:以氯化胆碱/乙二醇按1∶3的摩尔比合成的低共熔溶剂(含水量23%,g/g)作提取试剂,提取时间30 min,提取温度80℃,液料比58∶1(mL/g)。结论 低共熔溶剂比传统试剂在金钱草总黄酮的提取效果上具有明显优势。本方法操作简单,节能环保,提取效果良好,为总黄酮的提取工艺研究提供了新思路。
引文
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[2] 周军,韦桂宁,吴超伟,等.复方金钱草颗粒对肾结石的影响及其利尿、解痉、抗炎作用[J].中国实验方剂学杂志,2011,17(18):206.
[3] 张春丽.单味金钱草治疗胆石症胆囊炎 28例疗效观察[J].时珍国医与国药,2000,11(10):936.
[4] 齐俊英 ,魏晨玲 ,吴文博.舒肝利胆排石丸的制备与临床观察[J].时珍国医与国药,2008,19(7):1687.
[5] 李军,蔡泓,王君明,等.金钱草化学成分、药理作用及临床应用[J].中国老年学杂志,2017,37(24):6262.
[6] 邹志辉,崔维奇,谌辉鹏,等.金钱草黄酮提取物对大鼠肾脏草酸钙结石形成的影响[J].中国实验方剂学杂志,2013,19(4):195.
[7] Hirayama H,Wang Z,Nishi K,et al.Effect of Desmodium styracifolium-triterpenoid on calcium oxalate renal stones[J].Br J Urol,1993,71(2):143.
[8] Zhou J,Jin J,Li X,et al.Total flavonoids of Desmodium styracifolium attenuates the formation of hydroxyⅠproline induced calcium oxalate urolithiasis in rats[J].Urolithiasis,2018,46 (3):231.
[9] 许实波,丘晨波,钟如芸,等.广金钱草总黄酮对心脑血管的效应[J].中山大学学报(自然科学版) ,1980,29(4):98.
[10] Ho C S,Wong Y H,Chiu K W.The hypotensive action of Desmodium styracifolium and Clematis chinensis[J].Am J Chin Med,1989,17(3/4):189.
[11] 郑占虎,董泽宏,佘靖.中药现代研究与应用[M].北京:学苑出版社,1998:2921.
[12] 阎婷,李益生.金钱草总黄酮的抗炎活性[J].中国老年学杂志,2015,35(19):5394.
[13] 王晓林,钟方丽,李敦猛,等.广金钱草中总黄酮提取工艺研究[J].中药材,2008,31(6):900.
[14] 陈小英,张瑞辉,黎婉红,等.对广金钱草不同部位总黄酮的含量分析[J].中国医药指南,2014,12(13):77.
[15] 罗兰,张素中,黄月纯,等.广金钱草总黄酮成分不同提取方法的比较[J].中成药,2015,37(11):2537.
[16] Dai Y T,Spronsen J V,Witkampb G J,et al.Natural deep eutectic solvents as new potential media for green technology[J].Anal Chim Acta,2013,766:61.
[17] 熊兴泉,韩塞,石霖,等.低共熔溶剂在绿色有机合成中的应用[J].有机化学,2016,3(36):480.
[18] Duan L,Dou L L,Guo L,et al.Comprehensive Evaluation of Deep Eutectic Solvents in Extraction of Bioactive Natural Products[J].ACS Sustainable Chem Eng,2016,4:2405.
[19] Wei Z F,Wang X Q,Xiao P,et al.Fast and green extraction and separation of main bioactive flavonoids from Radix Scutellariae[J].Ind Crop Prod,2015,63:175.
[20] Wang M,Wang J Q,Zhang Y,et al.Fast environment-friendly ball mill-assisted deep eutectic solvent-based extraction of natural products[J].J Chromatogr A,2016,1443:262.
[21] Dai Y T,Verpoorte R ,Choi Y H.Natural deep eutectic solvents providing enhanced stability of natural colorants from safflower (Carthamustinctorius) [J].Food Chem,2014,159:116.
[22] Dai Y T,Rozema E,Verpoorte R,et al.Application of natural deep eutectic solvents to the extraction of anthocyanins from Catharanthusroseus with high extractability and stability replacing conventional organic solvents [J].J Chromatogr A,2016,1434:50.