响应面法优化铜藻中岩藻黄素的提取工艺
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摘要
目的采用响应面法优化铜藻中岩藻黄素的乙醇溶液提取工艺。方法用乙醇溶液作提取溶剂,采用超声法提取铜藻中的岩藻黄素,研究乙醇浓度、料液比、提取温度、提取时间对岩藻黄素提取的影响,在单因素试验的基础上,用Box-Benhnken中心组合试验设计迚行4因素3水平的响应面分析,以岩藻黄素的提取量为响应值,优化提取工艺参数。结果响应面试验得到超声提取最佳条件:料液比1:100、起始温度60℃、超声时间40 min、乙醇浓度70%。在优化的条件下,超声提取岩藻黄素提取量为1092μg/g,和预测值1183μg/g基本一致。结论通过响应面优化,得到了乙醇溶液超声提取铜藻中岩藻黄素的最佳条件,提高了岩藻黄素的提取量。
Objective To optimize the extracting conditions of fucoxanthin from Sargassum horneri by response surface methodology(RSM). Methods Fucoxanthin was extracted from Sargassum horneri with ethanol as solvent by ultrasonication. The effects of ethanol concentration, solid-liquid ratio, extraction temperature and time on the extraction of fucoxanthin from Sargassum horneri were studied. Based on the results of single factor experiments,Box-Benhnken design center combination method of 4 factors and 3 levels experimental design was applied to further optimize the extraction conditions using the extraction yield as response value. Results The response surface interaction analysis showed that the optimum extraction conditions for ultrasonic extraction were solid-liquid ratio at1:100, initial temperature at 60 ℃, extraction time at 40 min and ethanol concentration at 70%. Under the optimized conditions, the extraction yield of fucoxanthin from Sargassum horneri by ultrasonic extraction was up to 1092 μg/g,which was highly consistent with the predictive value of 1183 μg/g. Conclusion By response surface optimization,the optimum conditions for ultrasonic extraction of fucoxanthin from Sargassum horneri by ethanol solution were obtained, which increased the extraction amount of fucoxanthin.
Objective To optimize the extracting conditions of fucoxanthin from Sargassum horneri by response surface methodology(RSM). Methods Fucoxanthin was extracted from Sargassum horneri with ethanol as solvent by ultrasonication. The effects of ethanol concentration, solid-liquid ratio, extraction temperature and time on the extraction of fucoxanthin from Sargassum horneri were studied. Based on the results of single factor experiments,Box-Benhnken design center combination method of 4 factors and 3 levels experimental design was applied to further optimize the extraction conditions using the extraction yield as response value. Results The response surface interaction analysis showed that the optimum extraction conditions for ultrasonic extraction were solid-liquid ratio at1:100, initial temperature at 60 ℃, extraction time at 40 min and ethanol concentration at 70%. Under the optimized conditions, the extraction yield of fucoxanthin from Sargassum horneri by ultrasonic extraction was up to 1092 μg/g,which was highly consistent with the predictive value of 1183 μg/g. Conclusion By response surface optimization,the optimum conditions for ultrasonic extraction of fucoxanthin from Sargassum horneri by ethanol solution were obtained, which increased the extraction amount of fucoxanthin.
引文
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[14]李红艳,刘天红,姜晓东,等.超声辅助乙醇提取铜藻中岩藻黄素的工艺研究[J].中国农业科技导报,2018,20(9):146-153.Li HY,Liu TH,Jiang XD,et al.Optimization of ultrasonic enhanced extraction of fucoxanthin from Sargassum horneri[J].J Agric Sci Technol,2018,20(9):146-153.
[15]Masaru T,Chikara K,Atsushi I,et al.Spatial and seasonal variations in the biofunctional lipid substances(fucoxanthin and fucosterol)of the laboratory-grown edible Japanese seaweed(Sargassum horneri Turner)cultured in the open sea[J].Saudi J Biolog Sci,2017,(24):1475-1482.
[2]张文源,高保燕,雷学青,等.岩藻黄素的理化与生物学特性、制备技术及其生理活性研究迚展[J].中国海洋药物,2015,34(3):81-95.Zhang WY,Gao BY,Lei XQ,et al.Progress on physicochemical and biological properties,preparation techniques and physiological activities of fucoxanthin[J].Chin J Marine Drugs,2015,34(3):81-95.
[3]Xia S,Wang K,Wan L,et al.Production,characterization,and antioxidant activity of fucoxanthin from the marine diatom Odontella aurita[J].Mar Drug,2013,11(7):2667-2681.
[4]Eko S,Akhmad SF,Masayuki A,et al.Lipids,fatty acids,and fucoxanthin content from temperate and tropical brown seaweeds[J].Aquatic Proc,2016,(7):66-75.
[5]曾呈奎.中国常见海藻[M].北京:科学出版社,1983.Zeng CK.Common seaweeds in China[M].Beijing:Science Press,1983.
[6]孙建璋,陈万东,庄定根,等.中国南麂列岛铜藻Sargassum horneri实地生态学的初步研究[J].南方水产,2008,4(3):58-63.Sun JZ,Chen WD,Zhuang DG,et al.In situ ecological studies of the subtidal brown alga Sargasssum horneri at Nanji island of China[J].South China Fish Sci,2008,4(3):58-63.
[7]Lin Q,Hu C,Wang M,et al.Floating algae blooms in the east China sea[J].Geophys Res Lett,2017,44(22):11501-11509.
[8]Xing Q,Guo R,Wu L,et al.High-Resolution satellite observations of a new hazard of golden tides caused by floating sargassum in winter in the yellow sea[J].IEEE Geosci Remote Sensing Lett,2017,14(10):1815-1819.
[9]Liu F,Liu XF,Wang Y,et al.Insights on the Sargassum horneri golden tides in the Yellow Sea inferred from morphological and molecular data[J].Limnol Oceanogr,2018,63(4):1762-1773.
[10]苌钊,徐小琴,李强,等.超声提取裙带菜中岩藻黄素的工艺研究[J].药物评价研究,2013,36(4):285-288.Chang Z,Xu XQ,Li Q,et al.Optimization of ultrasonic enhanced extraction of fucoxanthin from Undaria pinnatifida[J].Drug Evaluat Res,2013,36(4):285-288.
[11]顾晓慧,陈志兵,徐梅双,等.响应面优化海带岩藻黄素超声提取工艺研究[J].食品工业,2018,39(2):169-172.Gu XH,Chen ZB,Xu MS,et al.Optimization of ultrasound-assisted extraction technology of fucoxanthin from kelp by response surface methodology[J].Food Ind,2018,39(2):169-172.
[12]王乐,李丼,牟海津.鼠尾藻岩藻黄素超声辅助提取及其纯化工艺优化[J].食品工业科技,2018,39(8):141-146.Wang L,Li L,Mou HJ.Optimization of ultrasonic assisted extraction and purification of fucoxanthin from Sargassum thunbergii[J].Sci Technol Food Ind,2018,39(8):141-146.
[13]胡永东,吴柠,李易珍,等.铜藻岩藻黄质的提取工艺优化[J].浙江海洋学院学报(自然科学版),2014,33(6):501-505,510.Hu YD,Wu N,Li YZ,et al.Study on extraction of fucoxanthin from Sargassum horneri[J].J Zhejiang Ocean Univ(Nat Sci Ed),2014,33(6):501-505,510.
[14]李红艳,刘天红,姜晓东,等.超声辅助乙醇提取铜藻中岩藻黄素的工艺研究[J].中国农业科技导报,2018,20(9):146-153.Li HY,Liu TH,Jiang XD,et al.Optimization of ultrasonic enhanced extraction of fucoxanthin from Sargassum horneri[J].J Agric Sci Technol,2018,20(9):146-153.
[15]Masaru T,Chikara K,Atsushi I,et al.Spatial and seasonal variations in the biofunctional lipid substances(fucoxanthin and fucosterol)of the laboratory-grown edible Japanese seaweed(Sargassum horneri Turner)cultured in the open sea[J].Saudi J Biolog Sci,2017,(24):1475-1482.