高效液相色谱法测定纳米海绵中游离甲醛含量
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摘要
建立了高效液相色谱法(high performance liquid chromatography,HPLC)测定纳米海绵中游离甲醛含量的分析方法。以2, 4-二硝基苯肼为衍生剂与甲醛反应生成2, 4-二硝基苯腙。在352nm波长处,HPLC测定,外标法定量。该方法在0.02～20mg/L浓度范围内具有良好线性,相关系数(r~2)为0.9999,相对标准偏差小于5%。该方法操作简单、灵敏度高、重复性好,可用于纳米海绵中游离甲醛含量的测定。
To establish a method for determination of free formaldehyde content in nano-sponge by high performance liquid chromatography(HPLC). The 2, 4-dinitrophenylhydrazine was used as a derivatizing agent to react with formaldehyde to form 2, 4-dinitrophenylhydrazine. At the wavelength of 352 nm, the sample was determined by HPLC and quantified by external standard method. This method had good linearity in the concentration range of 0.02-20 mg/L, the correlation coefficient(r~2) was 0.9999, and the relative standard deviation(RSD) was less than 5%. This method is simple in operation, high in sensitivity and good in repeatability, which can be used for the determination of free formaldehyde content in nano-sponges.
To establish a method for determination of free formaldehyde content in nano-sponge by high performance liquid chromatography(HPLC). The 2, 4-dinitrophenylhydrazine was used as a derivatizing agent to react with formaldehyde to form 2, 4-dinitrophenylhydrazine. At the wavelength of 352 nm, the sample was determined by HPLC and quantified by external standard method. This method had good linearity in the concentration range of 0.02-20 mg/L, the correlation coefficient(r~2) was 0.9999, and the relative standard deviation(RSD) was less than 5%. This method is simple in operation, high in sensitivity and good in repeatability, which can be used for the determination of free formaldehyde content in nano-sponges.
引文
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[11]赵新淮,孙晶玮,姚良.食品中微量甲醛的化学分析研究[J].食品工业科技,2004,25(5):124-126.
[12]邵晓东,宋正华.流动注射化学发光快速测定空气中甲醛[J].光谱实验室,2006,23(5):1113-1118.
[13]张春玲.高效液相色谱法测定纺织品中游离甲醛含量[J].现代测量与实验室管理,2007,(4):11-13.
[14]陈润,王治洋,秦勇,等.溶剂极性控制下的煤抽余物吸附甲烷能力研究[J].燃料化学学报,2015,43(10):1153-115
[15]王相承,柯佳雄,凌育梅.溶解度法分离邻、对硝基氯苯的研究[J].化工时刊,2006,20(9):1-3+13.
[16]GB 31604.1-2016食品安全国家标准食品接触材料及制品迁移试验通则[S].
[17]GB 9685-2016食品安全国家标准食品接触材料及制品用添加剂使用标准[S].
[2]徐向荣,徐增康.甲醛的危害及其卫生检验方法[J].职业与健康,2003,19(12):47-49.
[3]王利英,杨振德.甲醛环境危害综述[J].安徽预防医学杂志,2006,12(13):178-181.
[4]朱桂芝,李涛,郭晓涛,等.甲醛对人体毒作用[J].中国卫生工程学,2004,3(4):250-251.
[5]安从俊,丁哨兵,杨波,等.室内空气环境中微(痕)量甲醛的主要分析方法[J].武汉大学学报(理学版),2001,4(47):433-437.
[6]张瑾.甲醛毒性的研究进展[J].职业与健康,2006,22(23):2041-2044.
[7]李紫薇.流动注射分光光度法测甲醛[J].伊犁师范学院学报(自然科学版),2007,3(1):35-37.
[8]黄晓兰,黄芳,林晓珊,等.气相色谱-质谱法测定食品中的甲醛[J].分析化学,2004,32(12):617-1620.
[9]吴新华,朱瑞芝,陆舍铭,等.高效液相色谱法测定香精中甲醛的含量[J].化学试剂,2010,32(9):817-818.
[10]何小青.直接蒸馏滴定-AHMT法联合测定食品中的甲醛次硫酸氢钠[J].食品与发酵工业,2004,30(3):106-108.
[11]赵新淮,孙晶玮,姚良.食品中微量甲醛的化学分析研究[J].食品工业科技,2004,25(5):124-126.
[12]邵晓东,宋正华.流动注射化学发光快速测定空气中甲醛[J].光谱实验室,2006,23(5):1113-1118.
[13]张春玲.高效液相色谱法测定纺织品中游离甲醛含量[J].现代测量与实验室管理,2007,(4):11-13.
[14]陈润,王治洋,秦勇,等.溶剂极性控制下的煤抽余物吸附甲烷能力研究[J].燃料化学学报,2015,43(10):1153-115
[15]王相承,柯佳雄,凌育梅.溶解度法分离邻、对硝基氯苯的研究[J].化工时刊,2006,20(9):1-3+13.
[16]GB 31604.1-2016食品安全国家标准食品接触材料及制品迁移试验通则[S].
[17]GB 9685-2016食品安全国家标准食品接触材料及制品用添加剂使用标准[S].