摘要
目的:建立赤芝药材的高效液相指纹图谱并进行聚类分析。方法:收集不同产地、不同批号的10批赤芝药材,以灵芝酸A、灵芝酸G为参照物,采用ZORBAX SB-C_(18)(250 mm×4.6 mm,5μm)色谱柱;流动相:乙腈-0.2%磷酸水,梯度洗脱;流速1.0 mL·min~(-1);检测波长254 nm。利用"中药色谱指纹图谱相似度评价系统2004A"建立指纹图谱,评价图谱的相似性,并进行聚类分析。结果:建立了10批赤芝药材的HPLC指纹图谱,共标定31个共有色谱峰,方法学考察结果符合指纹图谱的技术要求,相同产地的药材基本可以聚为一类。结论:该方法稳定、可靠、重复性好,为灵芝药材的质量评价提供了新的方法。
Objective:To establish an HPLC fingerprint of Ganoderma lucidum.Method:10 batches of G.lucidum from different origins and batches were collected,ganoderic acid A and ganoderic acid were used as reference materials,the HPLC method was used in the study with ZORBAX SB-C18(250 mm×4.6 mm,5 μm)column and mobile phase of acetonitrile-0.2% phosphoric acid water,the flow rate was 1.0 mL·min~(-1),and detection wavelength was 254 nm.The similarity of the obtained fingerprints were evaluated by the Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System 2004 A.Results:The 10 batch of G.lucidum HPLC fingerprints were established,a total of 31 common peaks were calibrated,the method was validated.The medicinal materials of the same origin could be basically grouped into one class.Conclusion:The method is stable,reliable,reproducible,and provides a new method to evaluate the quality of G.lucidum.
引文
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