葛枣猕猴桃果糖激酶基因电子克隆和生物信息学分析
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摘要
目的:果糖激酶是促进果糖磷酸化关键代谢步骤重要的酶。通过电子克隆技术对葛枣猕猴桃果糖激酶基因进行预测。方法:以毛花猕猴桃果糖激酶序列为探针,基于美国国立生物技术信息中心(NCBI)中葛枣猕猴桃的EST数据库和CAP3在线软件进行序列拼接,利用生物信息学数据库及相关软件对其结构和功能进行预测分析。结果:葛枣猕猴桃果糖激酶基因全长1 015 bp,包含957 bp的开放阅读框,编码318个氨基酸,该蛋白为疏水性蛋白。结论:本研究为进一步解释葛枣猕猴桃果糖激酶基因的分子功能奠定理论及实验基础。
Objective: Fructokinases is an important enzyme catalyzing the key metabolic step of fructose phosphorylation. To obtain the fructokinase gene from Actinidia polygama by using in silico cloning. Methods: Fructokinases were cloned by ETS database and using the CAP3 bioinformatics software with Actinidia eriantha fructokinase sequence as a querying probe,and bioinformatics database and related software were used to predict the structure and function. Results: The full length of fructokinase gene was 1015 bp and contained a 957 bp ORF with 318 amino acids. The protein was predicted as a hydrophobic protein. Conclusion: The study lays theoretical and experimental basis for further explaining the molecular genetic function of fructokinase.
Objective: Fructokinases is an important enzyme catalyzing the key metabolic step of fructose phosphorylation. To obtain the fructokinase gene from Actinidia polygama by using in silico cloning. Methods: Fructokinases were cloned by ETS database and using the CAP3 bioinformatics software with Actinidia eriantha fructokinase sequence as a querying probe,and bioinformatics database and related software were used to predict the structure and function. Results: The full length of fructokinase gene was 1015 bp and contained a 957 bp ORF with 318 amino acids. The protein was predicted as a hydrophobic protein. Conclusion: The study lays theoretical and experimental basis for further explaining the molecular genetic function of fructokinase.
引文
1季华.葛枣叶的成分及效能的研究[J].林业科技,2001,26(6):54-56
2 Yoshizawa Y,Fukiya Y,Izumi Y,et al.Induction of apoptosis with an extract of actinidia polygama fruit in the promyelocytic leukemia cell line HL60[J].Journal of Health Science,2002,48(4):303-309
3 Nagai T,Tanoue Y,Kai N,et al.The liquor made from silver vine[Actinidia polygama(Sieb.et Zucc.)Planch.ex Maxim.]berries possess strongly antioxidative activity and antihypertensive activity[J].African Joumal of Food Science,2011,5(3):125-130
4 Asano T,Kunieda N,Omura Y,et al.RiceSPK,a calmodulin-like domain protein kinase,is required for storage product accumulation during seed development:phosphorylation of sucrose synthase is a possible factor.[J].Plant Cell,2002,14(3):619
5 Renz A,Stitt M.Substrate specificity and product inhibition of different forms of fructokinases and hexokinases in developing potato tubers[J].Planta,1993,190(2):166-175
6 Kanayama Y,Granot D,Dai N,et al.Tomato fructokinases exhibit differential expression and substrate regulation[J].Plant Physiology,1998,117(1):85-90
7 Guglielminetti L,Perata P,Morita A,et al.Characterization of isoforms of hexose kinases in rice embryo[J].Phytochemistry,2000,53(2):195-200
8 Pego JV,Smeekens SCM.Plant fructokinases:a sweet family get-together[J].Trends in Plant Science,2000,5(12):531-536
9 Huminiecki L,Bicknell R.In silico cloning of novel endothelial-specific genes[J].Genome Research,2000,10(11):1796-1806
10胡皝,萧浪涛.生物信息学在新基因全长cDNA电子克隆中的应用[J].生物技术通报,2007,2007(4):93-96
11 Gill RW,Sanseau P.Rapid in silico cloning of genes using expressed sequence tags(ESTs)[J].Biotechnology Annual Review,2000,5:25-44
12王冬冬,朱延明,李勇,等.电子克隆技术及其在植物基因工程中的应用[J].东北农业大学学报,2006,37(3):403-408
13张开雪,任伟超,闫嵩,等.软枣猕猴桃GDP-L-半乳糖磷酸酶电子克隆与生物信息学分析[J].中国药师,2016,19(11):2066-2069
14闫嵩,任伟超,刘振鹏,等.百脉根细胞亲环素电子克隆和生物信息学分析[J].中草药,2016,47(19):3481-3485
15任伟超,温东,赵锐,等.美洲茶藨子查耳酮合成酶基因电子克隆和生物信息学分析[J].中国药师,2017,20(12):2106-2109
16 PHuang X,Madan A.CAP3:A DNA sequence assembly program[J].Genome Research,1999,9(9):868-877
17 Gasteiger E,Hoogland C,Gattiker A,et al.Protein identification and analysis tools on the ExPASy server[M].Totowa:Humana Press,2005:571-607
18 Petersen TN,Brunak S,von Heijne G,et al.SignalP 4.0:discriminating signal peptides fromtransmembrane regions[J].Nature Methods,2011,8(10):785-786
19 Kyte J,Doolittle RF.A simple method for displaying the hydropathic character of a protein[J].Journal of Molecular Biology,1982,157(1):105-132
20 Hofmann K,Stoffel W.TMbase-A database of membrane spanning protein segments[J].Biol Chem Hoppe-Seyler,1993,374(1):1-3
21 Nakai K,Horton P.PSORT:a program for detecting sorting signals in proteins and predicting their subcellular localization[J].Trends in Biochemical Sciences,1999,24(1):34-36
22 Geourjon C,Deleage G.SOPMA:significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments[J].Comput Appl Biosci,1995,11(6):681-684
23 Blom N,Gammeltoft S,Brunak S.Sequence and structure-based prediction of eukaryotic protein phosphorylation sites[J].Journal of Molecular Biology,1999,294(5):1351-1362
24崔娜,王卫平,林凤,等.植物果糖激酶的研究进展[J].中国农学通报,2010,26(14):41-47
25 Zhang S,Nichols SE,Dong JG.Cloning and characterization of two fructokinases from maize[J].Plant Science,2003,165(5):1051-1058
26 Jiang H,Dian W,Liu F,et al.Isolation and characterization of two fructokinase cDNA clones from rice[J].Phytochemistry,1992,1(1):5-10
27 Qin Q,Zhang S,Chen J,et al.Isolation and expression analysis of fructokinase genes from citrus[J].Acta Botanica Sinica,2004,46(12):1408-1414
28 Taylor MA,Ross HA,Gardner A,et al.Characterisation of a cDNA encoding fructokinase from potato(solanum tuberosum L.)[J].Journal of Plant Physiology,1995,145:253-256
29 Kanayama Y,Dai N,Granot D,et al.Divergent fructokinase genes are differentially expressed in tomato[J].Plant Physiology,1997,113(4):1379-1384
2 Yoshizawa Y,Fukiya Y,Izumi Y,et al.Induction of apoptosis with an extract of actinidia polygama fruit in the promyelocytic leukemia cell line HL60[J].Journal of Health Science,2002,48(4):303-309
3 Nagai T,Tanoue Y,Kai N,et al.The liquor made from silver vine[Actinidia polygama(Sieb.et Zucc.)Planch.ex Maxim.]berries possess strongly antioxidative activity and antihypertensive activity[J].African Joumal of Food Science,2011,5(3):125-130
4 Asano T,Kunieda N,Omura Y,et al.RiceSPK,a calmodulin-like domain protein kinase,is required for storage product accumulation during seed development:phosphorylation of sucrose synthase is a possible factor.[J].Plant Cell,2002,14(3):619
5 Renz A,Stitt M.Substrate specificity and product inhibition of different forms of fructokinases and hexokinases in developing potato tubers[J].Planta,1993,190(2):166-175
6 Kanayama Y,Granot D,Dai N,et al.Tomato fructokinases exhibit differential expression and substrate regulation[J].Plant Physiology,1998,117(1):85-90
7 Guglielminetti L,Perata P,Morita A,et al.Characterization of isoforms of hexose kinases in rice embryo[J].Phytochemistry,2000,53(2):195-200
8 Pego JV,Smeekens SCM.Plant fructokinases:a sweet family get-together[J].Trends in Plant Science,2000,5(12):531-536
9 Huminiecki L,Bicknell R.In silico cloning of novel endothelial-specific genes[J].Genome Research,2000,10(11):1796-1806
10胡皝,萧浪涛.生物信息学在新基因全长cDNA电子克隆中的应用[J].生物技术通报,2007,2007(4):93-96
11 Gill RW,Sanseau P.Rapid in silico cloning of genes using expressed sequence tags(ESTs)[J].Biotechnology Annual Review,2000,5:25-44
12王冬冬,朱延明,李勇,等.电子克隆技术及其在植物基因工程中的应用[J].东北农业大学学报,2006,37(3):403-408
13张开雪,任伟超,闫嵩,等.软枣猕猴桃GDP-L-半乳糖磷酸酶电子克隆与生物信息学分析[J].中国药师,2016,19(11):2066-2069
14闫嵩,任伟超,刘振鹏,等.百脉根细胞亲环素电子克隆和生物信息学分析[J].中草药,2016,47(19):3481-3485
15任伟超,温东,赵锐,等.美洲茶藨子查耳酮合成酶基因电子克隆和生物信息学分析[J].中国药师,2017,20(12):2106-2109
16 PHuang X,Madan A.CAP3:A DNA sequence assembly program[J].Genome Research,1999,9(9):868-877
17 Gasteiger E,Hoogland C,Gattiker A,et al.Protein identification and analysis tools on the ExPASy server[M].Totowa:Humana Press,2005:571-607
18 Petersen TN,Brunak S,von Heijne G,et al.SignalP 4.0:discriminating signal peptides fromtransmembrane regions[J].Nature Methods,2011,8(10):785-786
19 Kyte J,Doolittle RF.A simple method for displaying the hydropathic character of a protein[J].Journal of Molecular Biology,1982,157(1):105-132
20 Hofmann K,Stoffel W.TMbase-A database of membrane spanning protein segments[J].Biol Chem Hoppe-Seyler,1993,374(1):1-3
21 Nakai K,Horton P.PSORT:a program for detecting sorting signals in proteins and predicting their subcellular localization[J].Trends in Biochemical Sciences,1999,24(1):34-36
22 Geourjon C,Deleage G.SOPMA:significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments[J].Comput Appl Biosci,1995,11(6):681-684
23 Blom N,Gammeltoft S,Brunak S.Sequence and structure-based prediction of eukaryotic protein phosphorylation sites[J].Journal of Molecular Biology,1999,294(5):1351-1362
24崔娜,王卫平,林凤,等.植物果糖激酶的研究进展[J].中国农学通报,2010,26(14):41-47
25 Zhang S,Nichols SE,Dong JG.Cloning and characterization of two fructokinases from maize[J].Plant Science,2003,165(5):1051-1058
26 Jiang H,Dian W,Liu F,et al.Isolation and characterization of two fructokinase cDNA clones from rice[J].Phytochemistry,1992,1(1):5-10
27 Qin Q,Zhang S,Chen J,et al.Isolation and expression analysis of fructokinase genes from citrus[J].Acta Botanica Sinica,2004,46(12):1408-1414
28 Taylor MA,Ross HA,Gardner A,et al.Characterisation of a cDNA encoding fructokinase from potato(solanum tuberosum L.)[J].Journal of Plant Physiology,1995,145:253-256
29 Kanayama Y,Dai N,Granot D,et al.Divergent fructokinase genes are differentially expressed in tomato[J].Plant Physiology,1997,113(4):1379-1384