线辣椒成熟果色黄色性状遗传规律
|
摘要
以线辣椒黄色突变体H0809为母本材料,野生型XHB(红色)及红色高代自交系CP1211-1、CP12332-5、CP1235-11、 CP1245-6、CP1256-10、CP1271-3为父本材料,配制杂交组合,对亲本、不同组合F_1代及F_2代分离群体的果实颜色性状进行调查与分析。结果表明,各组合F_1代群体辣椒果实颜色均为红色,说明辣椒果实红色对黄色为显性, F_2代分离群体红色和黄色单株数量符合孟德尔3∶1的分离比例,表明线辣椒成熟果色黄色性状受1对隐性基因控制,黄色线辣椒H0809为红色野生型XHB的隐形突变。
In the present study,the wild type XHB(red) and red high-generation inbred lines CP1211-1,CP12332-5,CP1235-11,CP1245-6,CP1256-10 and CP1271-3 were used as male parents while the yellow pepper mutant H0809 as female parent material,to investigate the color traits of the parents,hybridized combinations F_1 and F_2.The results showed that the color of each combination F_1 generation group was red,indicating that the red pepper fruit color was dominant to yellow color.The red and yellow individuals of F_2 segregating population were in accordance with Mendel's 3∶1 separation ratio,indicating that the yellow ripe fruit of chili pepper was controlled by one recessive genes,the yellow line pepper H0809 was a stealthy mutation of the red wild-type XHB.
In the present study,the wild type XHB(red) and red high-generation inbred lines CP1211-1,CP12332-5,CP1235-11,CP1245-6,CP1256-10 and CP1271-3 were used as male parents while the yellow pepper mutant H0809 as female parent material,to investigate the color traits of the parents,hybridized combinations F_1 and F_2.The results showed that the color of each combination F_1 generation group was red,indicating that the red pepper fruit color was dominant to yellow color.The red and yellow individuals of F_2 segregating population were in accordance with Mendel's 3∶1 separation ratio,indicating that the yellow ripe fruit of chili pepper was controlled by one recessive genes,the yellow line pepper H0809 was a stealthy mutation of the red wild-type XHB.
引文
[1]戴雄泽,王利群,陈文超,等.辣椒果实发育过程中果色与类胡萝卜素的变化[J].中国农业科学,2009,42(11):4004-4011.DAI X Z,WANG L Q,CHEN W CH,et al.Changes of fruit colors and carotenoid contents during the development of pepper fruit[J].Scientia Agricultura Sinica,2009,42(11):4004-4011.
[2]GOMEZ-GARCIA,MDEL R,OCHOA-ALEJO N.Biochemistry and molecular biology of carotenoid biosynthesis in chili peppers(Capsicumspp.)[J].International Journal of Molecular Sciences,2013,14(9):19025-19053.
[3]AZA-GONZALEZ C,PALENIUS H G OCHOA-ALE-JON.Molecular biology of chili pepper anthocyanin biosynthesis[J].Journal of the Mexican Chemical Society,2012,56(1):93-98.
[4]RANJITH A,RAMESH B,NATARAJAN,et al.Red pepper(Capsicum annuum)carotenoids as a source of natural food colors:analysis and stability-a review[J].Journal of Food Science and Technology,2015,52(3):1258-1271.
[5]DESPHANDE R B.Studies in Indians chillies:the inheritance of some characters in Capsicum annuum L[J].Indian Journal of Agriculture,1933,3:299-300.
[6]SMITH P G.Inheritance of brown and green mature fruit color in peppers[J].Journal of Heredity,1950,41:138-140.
[7]KORMOS J,KORMOS K.Die genetischen typen der carotinoid-systems der paprikafrucht[J].Acta Botanica,1960,6:305-319.
[8]HERNANDEZ H H,SMITH P G.Inheritance of mature fruit color in Capsicum annuum L[J].Journal of Heredity,1985,76:211-213.
[9]SHIFRISS C,PILOVSKY M.Studies of the inheritance of mature fruit color in Capsicum annuum L[J].Euphytica,1992,60:123-126.
[10]POPOVSKY S,PARAN I.Molecular genetics of the ylocus in pepper:its relation to capsanthin-capsorubin synthase and to fruit color[J].Theoretical and Applied Genetics,2000,10:86-89.
[11]THORUP T A,TANYOLAC B,LIVINGSTONE K D,et al.Candidate gene analysis of organ pigmentation loci in the Solanaceae[J].Proceedings of the National Academy of Sciences of the United States of America,2000,97(21):11192-11197.
[12]KIM O R,CHO M C,KIM B D,et al.A splicing mutation in the gene encoding phytoene synthase causes orange coloration in Habanero pepper fruits[J].Molecules and Cells,2010,30:569-574.
[13]HUH J H,KANG B C,NAHM S H,et al.A candidate gene approach identifies phytoene synthase as the locus for mature fruit color in red pepper(Capsicum spp.)[J].Theoretical and Applied Genetics,2001,102:24-530.
[14]郭爽,黄贞,常绍东,等.辣椒果实类胡萝卜素研究进展[J].中国蔬菜,2012(4):19-24.GUO SH,HUANG ZH,CHANG SH D,et al.Research progress on carotenoid in pepper fruit[J].China Vegetables,2012(4):19-24.
[15]LEFEBVRE V,KUNTZ M,CAMARA B,et al.The capsanthin-capsorubin synthase gene:a candidate gene for the y locus controlling the red fruit color in pepper[J].Plant Molecular Biology,1998,36:785-789.
[16]HA S H,KIM J B,PARK J S,et al.A comparison of the carotenoid accumulation in Capsicum varieties that show different ripening colours:deletion of the capsanthin-capsorubin synthase gene is not a prerequisite for the formation of a yellow pepper[J].Journal of Experimental Botany,2007,58:3135-3144.
[17]LAURA R U,IVETTE G,WATHSALA R,et al.Carotenoid accumulation in orange-pigmented Capsicum annuum fruit,regulated at multiple levels[J].Journal of Experimental Botany,2012,63(1):517-526.
[18]LANG Y,YANAGAWA S,SASANUMA T,et al.Orange fruit colour in Capsicum due to deletion of capsanthincapsorubin synthesis gene[J].Breeding Science,2004,54:33-39.
[19]BOROVSKY Y,TADMOR Y,BAR E,et al.Induced mutation inβ-carotene hydroxylase results in accumulation ofβ-carotene and conversion of red to orange color in pepper fruits[J].Theoretical and Applied Genetics,2013,126:557-565.
[20]BARRY C S,MCQUINN R P,CHUNG M Y,et al.Amino acid substitutions in homologs of the STAY-GREEN protein are responsible for the green-flesh and chlorophyll retainer mutations of tomato and pepper[J].Plant Physiology,2008,147(1):179-187.
[21]BOROVSKY Y,PARAN I.Chlorophyll breakdown during pepper fruit ripening in the chlorophyll retainer mutation is impaired at the homolog of the senescence-inducible stay-green gene[J].Theoretical and Applied Genetics,2008,117(2):235-240.
[2]GOMEZ-GARCIA,MDEL R,OCHOA-ALEJO N.Biochemistry and molecular biology of carotenoid biosynthesis in chili peppers(Capsicumspp.)[J].International Journal of Molecular Sciences,2013,14(9):19025-19053.
[3]AZA-GONZALEZ C,PALENIUS H G OCHOA-ALE-JON.Molecular biology of chili pepper anthocyanin biosynthesis[J].Journal of the Mexican Chemical Society,2012,56(1):93-98.
[4]RANJITH A,RAMESH B,NATARAJAN,et al.Red pepper(Capsicum annuum)carotenoids as a source of natural food colors:analysis and stability-a review[J].Journal of Food Science and Technology,2015,52(3):1258-1271.
[5]DESPHANDE R B.Studies in Indians chillies:the inheritance of some characters in Capsicum annuum L[J].Indian Journal of Agriculture,1933,3:299-300.
[6]SMITH P G.Inheritance of brown and green mature fruit color in peppers[J].Journal of Heredity,1950,41:138-140.
[7]KORMOS J,KORMOS K.Die genetischen typen der carotinoid-systems der paprikafrucht[J].Acta Botanica,1960,6:305-319.
[8]HERNANDEZ H H,SMITH P G.Inheritance of mature fruit color in Capsicum annuum L[J].Journal of Heredity,1985,76:211-213.
[9]SHIFRISS C,PILOVSKY M.Studies of the inheritance of mature fruit color in Capsicum annuum L[J].Euphytica,1992,60:123-126.
[10]POPOVSKY S,PARAN I.Molecular genetics of the ylocus in pepper:its relation to capsanthin-capsorubin synthase and to fruit color[J].Theoretical and Applied Genetics,2000,10:86-89.
[11]THORUP T A,TANYOLAC B,LIVINGSTONE K D,et al.Candidate gene analysis of organ pigmentation loci in the Solanaceae[J].Proceedings of the National Academy of Sciences of the United States of America,2000,97(21):11192-11197.
[12]KIM O R,CHO M C,KIM B D,et al.A splicing mutation in the gene encoding phytoene synthase causes orange coloration in Habanero pepper fruits[J].Molecules and Cells,2010,30:569-574.
[13]HUH J H,KANG B C,NAHM S H,et al.A candidate gene approach identifies phytoene synthase as the locus for mature fruit color in red pepper(Capsicum spp.)[J].Theoretical and Applied Genetics,2001,102:24-530.
[14]郭爽,黄贞,常绍东,等.辣椒果实类胡萝卜素研究进展[J].中国蔬菜,2012(4):19-24.GUO SH,HUANG ZH,CHANG SH D,et al.Research progress on carotenoid in pepper fruit[J].China Vegetables,2012(4):19-24.
[15]LEFEBVRE V,KUNTZ M,CAMARA B,et al.The capsanthin-capsorubin synthase gene:a candidate gene for the y locus controlling the red fruit color in pepper[J].Plant Molecular Biology,1998,36:785-789.
[16]HA S H,KIM J B,PARK J S,et al.A comparison of the carotenoid accumulation in Capsicum varieties that show different ripening colours:deletion of the capsanthin-capsorubin synthase gene is not a prerequisite for the formation of a yellow pepper[J].Journal of Experimental Botany,2007,58:3135-3144.
[17]LAURA R U,IVETTE G,WATHSALA R,et al.Carotenoid accumulation in orange-pigmented Capsicum annuum fruit,regulated at multiple levels[J].Journal of Experimental Botany,2012,63(1):517-526.
[18]LANG Y,YANAGAWA S,SASANUMA T,et al.Orange fruit colour in Capsicum due to deletion of capsanthincapsorubin synthesis gene[J].Breeding Science,2004,54:33-39.
[19]BOROVSKY Y,TADMOR Y,BAR E,et al.Induced mutation inβ-carotene hydroxylase results in accumulation ofβ-carotene and conversion of red to orange color in pepper fruits[J].Theoretical and Applied Genetics,2013,126:557-565.
[20]BARRY C S,MCQUINN R P,CHUNG M Y,et al.Amino acid substitutions in homologs of the STAY-GREEN protein are responsible for the green-flesh and chlorophyll retainer mutations of tomato and pepper[J].Plant Physiology,2008,147(1):179-187.
[21]BOROVSKY Y,PARAN I.Chlorophyll breakdown during pepper fruit ripening in the chlorophyll retainer mutation is impaired at the homolog of the senescence-inducible stay-green gene[J].Theoretical and Applied Genetics,2008,117(2):235-240.