鹅TTGGFFBBRRAAPP1基因克隆与表达分析
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摘要
为探讨TGFBRAP1在不同等级卵泡和不同就巢时期卵泡中的表达规律,通过RT-PCR、RACE技术克隆TGFBRAP1基因mRNA序列,并用RT-qPCR检测浙东白鹅不同等级卵泡以及不同就巢时期卵泡的TGFBRAP1表达。结果显示:TGFBRAP1 mRNA序列长为3 870 bp,其中包括45 bp的5′UTR和1 236 bp的3′UTR和2 589 bp的开放阅读框(Open reading frame,ORF),共编码862个氨基酸;RT-qPCR检测结果显示,等级卵泡中TGFBRAP1表达量均显著高于等级前卵泡(P<0.05),但均低于卵巢组织表达量,同时还发现产蛋期TGFBRAP1表达量显著高于就巢期(P<0.05)。研究表明,TGFBRAP1基因与鹅生殖调控密切相关,不仅参与了鹅卵泡发育,而且参与鹅产蛋/就巢发生。
引文
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[14]傅伟龙,陈鹭江,谢德麃.母鸡在排卵周期和抱窝血浆“黄体生成素类似物[J].华南农业大学学报,1991(S1):1-4.
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[2] CHARNG M J, ZHANG D, KINNUNEN P, et al. A novel protein distinguishes between quiescent and activated forms of the typeⅠtransforming growth factor beta receptor[J].Journal of Biological Chemistry, 1998, 273(16):9365.
[3] WURTHNER J U, FRANK D B, FELICI A, et al. Transforming growth factor-beta receptor-associated protein 1is a Smad4 chaperone[J]. Journal of Biological Chemistry,2001, 276(22):19495.
[4]王权,戚华兵,王晓凤,等.阻断TGF-β/TGF-βRⅠ通路对ATDC5细胞增殖、分化及细胞外基质的影响[J].第三军医大学学报,2013,35(10):917-921.
[5] MESSLER S, KROPP S, EPISKOPOU V, et al. The TGF-βsignaling modulators TRAP1/TGFBRAP1 and VPS39/Vam6/TLP are essential for early embryonic development[J]. Immunobiology, 2011, 216(3):343.
[6] DU L, GU T, ZHANG Y, et al. Transcriptome profiling to identify key mediators of granulosa cell proliferation upon FSH stimulation in the goose(Anser cygnoides)[J]. British Poultry Science, 2018.
[7] ABDOLLAH S, MAC?A?SSILVA M, TSUKAZAKI T, et al.TβRI phosphorylation of Smad2 on Ser465 and Ser467 is required for Smad2-Smad4 complex formation and signaling[J]. Journal of Biological Chemistry, 1997, 272(44):27678-85.
[8]王子遒,路洪涛,李书哲,等.鹅等级前卵泡中TGF-βⅠ和TGF-βRⅡ基因mRNA的表达分析[J].中国家禽,2017,39(7):6-10.
[9]吴慧英,贾汝敏,刘铀,等.鹅繁殖就巢机理研究进展[J].中国草食动物科学,2009,29(5):56-58.
[10]徐业芬. Smad信号转导通路相关基因表达与湖羊高繁殖力关系的研究[D].南京:南京农业大学,2010.
[11] ROY S K. Regulation of transforming growth factor-β-receptor typeⅠand typeⅡmessenger ribonucleic acid expression in the hamster ovary by gonadotropinsand steroid hormones1[J]. Biology of Reproduction, 2000, 62(6):1858-1865.
[12]程敏,闻良珍.转化生长因子β及其受体与卵泡发育[J].国际妇产科学杂志,2001,28(5):261-264.
[13]王光瑛,王寿昆.生殖激素对家禽就巢的调控[J].福建农林大学学报(自然版),2005,34(1):82-86.
[14]傅伟龙,陈鹭江,谢德麃.母鸡在排卵周期和抱窝血浆“黄体生成素类似物[J].华南农业大学学报,1991(S1):1-4.
[15]施振旦,梁少东,毕英佐.下丘脑多巴胺和5-羟色胺调控鸡就巢机理的研究[J].畜牧兽医学报,2000,31(6):487-492.