QuEChERS方法在法医毒物分析领域的研究进展
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摘要
在中毒或疑似中毒案件中,毒物鉴定对于提供侦查线索与犯罪证据、澄清案件性质、还原犯罪过程具有重要意义。基于液液萃取和分散固相萃取原理而建立起来的QuEChERS方法,由于其快速、简单、廉价、高效、可靠、安全等优势,正逐渐应用于法医毒物分析的样品前处理过程中。本文综述了QuEChERS方法的基本原理及其在提取和净化等过程中的优化改进,重点详述近年来QuEChERS方法在法医毒物分析领域的应用现状,并对未来发展方向进行展望。
In poisoning or suspected poisoning cases, the identification of toxicants is of great significance for providing clues and evidence, clarifying the nature of cases, and restoring the process of crime. Based on the principle of liquid-liquid extraction and dispersive solid-phase extraction, QuEChERS(Quick, Easy, Cheap, Effective, Rugged and Safe) method has been gradually applied to the pretreatment of biological samples. In this paper, the basic principle of QuEChERS and its optimization and improvement in the process of purification and extraction are summarized. Then the application of QuEChERS method in the field of forensic toxicological analysis in recent years is described in detail, and the future development is also forecasted.
In poisoning or suspected poisoning cases, the identification of toxicants is of great significance for providing clues and evidence, clarifying the nature of cases, and restoring the process of crime. Based on the principle of liquid-liquid extraction and dispersive solid-phase extraction, QuEChERS(Quick, Easy, Cheap, Effective, Rugged and Safe) method has been gradually applied to the pretreatment of biological samples. In this paper, the basic principle of QuEChERS and its optimization and improvement in the process of purification and extraction are summarized. Then the application of QuEChERS method in the field of forensic toxicological analysis in recent years is described in detail, and the future development is also forecasted.
引文
[1]González-Curbelo Má,Socas-Rodríguez B,HerreraHerrera A V,et al.Evolution and applications of the QuEChERS method[J].TrAC Trend Anal Chem,2015,71:169-185.
[2]Anastassiades M,Lehotay S J,?tajnbaher D,et al.Fast and easy multiresidue method employing acetonitrile extraction/partitioning and“dispersive solid-phase extraction”for the determination of pesticide residues in produce[J].J AOAC Int,2003,86(2):412-431.
[3]刘胜男,卫星,巩卫东.QuEChERS方法在检测分析中的应用研究进展[J].食品研究与开发,2013,34(10):133-136.
[4]曲斌.QuEChERS在动物源性食品兽药残留检测中的研究进展[J].食品科学,2013,34(5):327-331.
[5]陈建彪,董丽娜,刘娇,等.QuEChERS在食品中真菌毒素检测的研究进展[J].食品科学,2014,35(11):286-291.
[6]Lehotay S J.Determination of pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate:collaborative study[J].J AOAC Int,2007,90(2):485-520.
[7]Bruzzoniti M C,Checchini L,De Carlo R M,et al.QuEChERS sample preparation for the determination of pesticides and other organic residues in environmental matrices:a critical review[J].Anal Bioanal Chem,2014,406(17):4089-4116.
[8]Usui K,Hayashizaki Y,Minagawa T,et al.Rapid determination of disulfoton and its oxidative metabolites in human whole blood and urine using QuEChERSextraction and liquid chromatography-tandem mass spectrometry[J].Leg Med,2012,14(6):309-316.
[9]DeArmonda PD,Brittaina MK,Platoff Jrb GE,et al.QuEChERS-based approach toward the analysis of two insecticides,methomyl and aldicarb,in blood and brain tissue[J].Anal Methods,2015,7(1):321-328.
[10]Kim H S,Kim J,Suh J H,et al.General unknown screening for pesticides in whole blood and Korean gastric contents by liquid chromatography-tandem mass spectrometry[J].Arch Pharm Res,2014,37(10):1317-1324.
[11]Srivastava A,Rai S,Sonker A K,et al.Simultaneous determination of multiclass pesticide residues in human plasma using a mini QuEChERS method[J].Anal Bioanal Chem,2017,409(15):3757-3765.
[12]Yu T,Wang T,Huang Z,et al.Determination of Multiple Pesticides in Human Blood Using Modified QuEChERSMethod with Fe3O4 Magnetic Nanoparticles and GC-MS[J].Chromatographia,2017,80(1):165-170.
[13]Westland J L,Dorman F L.QuEChERS extraction of benzodiazepines in biological matrices[J].J Pharm Anal,2013,3(6):509-517.
[14]Anzillotti L,Odoardi S,Strano-Rossi S.Cleaning up blood samples using a modified"QuEChERS"procedure for the determination of drugs of abuse and benzodiazepines by UPLC-MS/MS[J].Forensic Sci Int,2014,243:99-106.
[15]冶廷祥.血液检材中常见安眠镇静类药物的QuEChERS处理与GC-MS分析[D].兰州:兰州大学,2016:12-14.
[16]Matsuta S,Nakanishi K,Miki A,et al.Development of a simple one-pot extraction method for various drugs and metabolites of forensic interest in blood by modifying the QuEChERS method[J].Forensic Sci Int,2013,232(13):40-45.
[17]徐多麒,张蕾萍,王继芬,等.超高效液相色谱串联质谱法同时快速检验全血中扎来普隆及其代谢产物[J].分析化学,2016,44(7):1059-106.
[18]魏万里.QuEChERS-HPLC-TOF-MS快速筛选测定全血中的21种常见催眠镇静药物.中国化学会.公共安全中的化学问题研究进展(第三卷)[C].中国化学会,2013:8.
[19]Usui K,Hayashizaki Y,Hashiyada M,et al.Rapid drug extraction from human whole blood using a modified QuEChERS extraction method[J].Leg Med,2012,14(6):286-296.
[20]Usui K,Hashiyada M,Hayashizaki Y,et al.Application of modified QuEChERS method to liver samples for forensic toxicological analysis[J].Forensic Toxicol,2014,32:139-147..
[21]Dulaurent S,El Balkhi S,Poncelet L,et al.QuEChERSsample preparation prior to LC-MS/MS determination of opiates,amphetamines,and cocaine metabolites in whole blood[J].Anal Bioanal Chem,2016,408(5):1467-1474.
[22]谢维平,欧阳燕玲,苏碧玲.QuEChERS净化-液相色谱串联质谱法测定血浆中8种抗凝血灭鼠剂[J].现代预防医学,2016,43(12):2229-2233.
[23]郁东,王德军,朱文文,等.QuEChERS结合气相色谱-质谱法快速测定职业工人尿液中多溴联苯醚[J].分析测试学报,2017,36(5):640-644.
[2]Anastassiades M,Lehotay S J,?tajnbaher D,et al.Fast and easy multiresidue method employing acetonitrile extraction/partitioning and“dispersive solid-phase extraction”for the determination of pesticide residues in produce[J].J AOAC Int,2003,86(2):412-431.
[3]刘胜男,卫星,巩卫东.QuEChERS方法在检测分析中的应用研究进展[J].食品研究与开发,2013,34(10):133-136.
[4]曲斌.QuEChERS在动物源性食品兽药残留检测中的研究进展[J].食品科学,2013,34(5):327-331.
[5]陈建彪,董丽娜,刘娇,等.QuEChERS在食品中真菌毒素检测的研究进展[J].食品科学,2014,35(11):286-291.
[6]Lehotay S J.Determination of pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate:collaborative study[J].J AOAC Int,2007,90(2):485-520.
[7]Bruzzoniti M C,Checchini L,De Carlo R M,et al.QuEChERS sample preparation for the determination of pesticides and other organic residues in environmental matrices:a critical review[J].Anal Bioanal Chem,2014,406(17):4089-4116.
[8]Usui K,Hayashizaki Y,Minagawa T,et al.Rapid determination of disulfoton and its oxidative metabolites in human whole blood and urine using QuEChERSextraction and liquid chromatography-tandem mass spectrometry[J].Leg Med,2012,14(6):309-316.
[9]DeArmonda PD,Brittaina MK,Platoff Jrb GE,et al.QuEChERS-based approach toward the analysis of two insecticides,methomyl and aldicarb,in blood and brain tissue[J].Anal Methods,2015,7(1):321-328.
[10]Kim H S,Kim J,Suh J H,et al.General unknown screening for pesticides in whole blood and Korean gastric contents by liquid chromatography-tandem mass spectrometry[J].Arch Pharm Res,2014,37(10):1317-1324.
[11]Srivastava A,Rai S,Sonker A K,et al.Simultaneous determination of multiclass pesticide residues in human plasma using a mini QuEChERS method[J].Anal Bioanal Chem,2017,409(15):3757-3765.
[12]Yu T,Wang T,Huang Z,et al.Determination of Multiple Pesticides in Human Blood Using Modified QuEChERSMethod with Fe3O4 Magnetic Nanoparticles and GC-MS[J].Chromatographia,2017,80(1):165-170.
[13]Westland J L,Dorman F L.QuEChERS extraction of benzodiazepines in biological matrices[J].J Pharm Anal,2013,3(6):509-517.
[14]Anzillotti L,Odoardi S,Strano-Rossi S.Cleaning up blood samples using a modified"QuEChERS"procedure for the determination of drugs of abuse and benzodiazepines by UPLC-MS/MS[J].Forensic Sci Int,2014,243:99-106.
[15]冶廷祥.血液检材中常见安眠镇静类药物的QuEChERS处理与GC-MS分析[D].兰州:兰州大学,2016:12-14.
[16]Matsuta S,Nakanishi K,Miki A,et al.Development of a simple one-pot extraction method for various drugs and metabolites of forensic interest in blood by modifying the QuEChERS method[J].Forensic Sci Int,2013,232(13):40-45.
[17]徐多麒,张蕾萍,王继芬,等.超高效液相色谱串联质谱法同时快速检验全血中扎来普隆及其代谢产物[J].分析化学,2016,44(7):1059-106.
[18]魏万里.QuEChERS-HPLC-TOF-MS快速筛选测定全血中的21种常见催眠镇静药物.中国化学会.公共安全中的化学问题研究进展(第三卷)[C].中国化学会,2013:8.
[19]Usui K,Hayashizaki Y,Hashiyada M,et al.Rapid drug extraction from human whole blood using a modified QuEChERS extraction method[J].Leg Med,2012,14(6):286-296.
[20]Usui K,Hashiyada M,Hayashizaki Y,et al.Application of modified QuEChERS method to liver samples for forensic toxicological analysis[J].Forensic Toxicol,2014,32:139-147..
[21]Dulaurent S,El Balkhi S,Poncelet L,et al.QuEChERSsample preparation prior to LC-MS/MS determination of opiates,amphetamines,and cocaine metabolites in whole blood[J].Anal Bioanal Chem,2016,408(5):1467-1474.
[22]谢维平,欧阳燕玲,苏碧玲.QuEChERS净化-液相色谱串联质谱法测定血浆中8种抗凝血灭鼠剂[J].现代预防医学,2016,43(12):2229-2233.
[23]郁东,王德军,朱文文,等.QuEChERS结合气相色谱-质谱法快速测定职业工人尿液中多溴联苯醚[J].分析测试学报,2017,36(5):640-644.