屠宰前禁食时间对肉鸡糖代谢、肉品质、血清生化和应激指标的影响
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摘要
本试验旨在研究屠宰前禁食时间对肉鸡糖代谢、肉品质、血清生化和应激指标的影响。试验选用42日龄爱拔益加(AA)肉鸡50羽,随机分为5组,每组10个重复,每个重复1羽。各组肉鸡屠宰前分别禁食0(对照)、6、12、18和24 h。结果表明:随着禁食时间的延长,血清葡萄糖、肝脏糖原和腿肌糖原含量呈线性和二次曲线显著降低(P<0.05)。随着禁食时间的延长,胸肌和腿肌pH_(24 h)均呈线性和二次曲线显著升高(P<0.05),胸肌和腿肌亮度值、蒸煮损失和剪切力呈线性和二次曲线显著降低(P<0.05)。随着禁食时间的延长,血清白蛋白、尿酸含量和丙氨酸氨基转移酶活性呈线性和二次曲线显著降低(P<0.05),血清天冬氨酸转氨酶、肌酸激酶活性和皮质酮含量呈线性和二次曲线显著升高(P<0.05)。结果提示,屠宰前禁食时间≥12 h会造成禁食应激加剧,合理的禁食时间(6～12 h)可以提高肉品质。
This study was conducted to evaluate the effects of pre-slaughter fasting time on carbohydrate metabolism,meat quality and serum biochemical and stress indexes of broilers. Fifty 42-day-old Arbor Acres (AA)broilers were randomly divided into 5 groups with 10 replicates per group and 1 broiler per replicate. The preslaughter fasting times in 5 groups were 0,6,12,18 and 24 h,respectively. The results showed that the contents of serum glucose,hepatic glycogen and muscle glycogen were significantly linear and quadratic decreased with fasting time extended (P<0.05). The pH_(24h) in breast muscle and leg muscle was significantly linear and quadratic increased with fasting time extended (P < 0. 05),and the lighteness value,cooking loss and shear force in breast muscle and leg muscle were significantly linear and quadratic decreased with fasting time extended (P<0.05). The albumin and uric acid contents and alanine aminotransferase activity in serum were significantly linear and quadratic decreased with fasting time extended (P<0.05),and the aspartate aminotransferase and creatine kinase activities and corticosterone content in serum were significantly linear and quadratic increased with fasting time extended (P<0.05). In conclusion,the pre-slaughter fasting time ≥12 h will intensify the fasting stress of broilers,the reasonable fasting time (6 to 12 h) can improve the meat quality.
This study was conducted to evaluate the effects of pre-slaughter fasting time on carbohydrate metabolism,meat quality and serum biochemical and stress indexes of broilers. Fifty 42-day-old Arbor Acres (AA)broilers were randomly divided into 5 groups with 10 replicates per group and 1 broiler per replicate. The preslaughter fasting times in 5 groups were 0,6,12,18 and 24 h,respectively. The results showed that the contents of serum glucose,hepatic glycogen and muscle glycogen were significantly linear and quadratic decreased with fasting time extended (P<0.05). The pH_(24h) in breast muscle and leg muscle was significantly linear and quadratic increased with fasting time extended (P < 0. 05),and the lighteness value,cooking loss and shear force in breast muscle and leg muscle were significantly linear and quadratic decreased with fasting time extended (P<0.05). The albumin and uric acid contents and alanine aminotransferase activity in serum were significantly linear and quadratic decreased with fasting time extended (P<0.05),and the aspartate aminotransferase and creatine kinase activities and corticosterone content in serum were significantly linear and quadratic increased with fasting time extended (P<0.05). In conclusion,the pre-slaughter fasting time ≥12 h will intensify the fasting stress of broilers,the reasonable fasting time (6 to 12 h) can improve the meat quality.
引文
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[2]GALLO C B,HUERTAS S M.Main animal welfare problems in ruminant livestock during preslaughter operations:a South American view[J].Animal,2016,10(2):357-364.
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[6]YANG C,HE J,CHEN D W,et al.Effects of corn type and fasting time before slaughter on grow th and plasma index in w eaning pigs[J].Journal of Animal Science,2016,94(1):106-116.
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[9]ZKAN S,AKBA爦Y,ALTAN,et al.The effect of short-term fasting on performance traits and rectal temperature of broilers during the summer season[J].British Poultry Science,2003,44(1):88-95.
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[16]PEREIRA R E P,MARTINS M R F B,MENDES AA,et al.Effects of pre-slaughter fasting on broiler w elfare,meat quality,and intestinal integrity[J].Revista Brasileira de Ciência Avícola,2013,15(2):119-122.
[17]GOTOH H,NOMURA T,ONO K.Glycogen serves as an energy source that maintains astrocyte cell proliferation in the neonatal telencephalon[J].Journal of Cerebral Blood Flow&M etabolism,2017,37(6):2294-2307.
[18]ADEVA-ANDANY M M,GONZLEZ-LUCN M,DONAPETRY-GARCA C,et al.Glycogen metabolism in humans[J].BBA Clinical,2016,5:85-100.
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[20]FLETCHER D L,QIAO M,SMITH D P.The relationship of raw broiler breast meat color and pH to cooked meat color and pH[J].Poultry Science,2000,79(5):784-788.
[21]徐昶.禁食和环境温度对鸡肉宰后僵直过程中能量代谢影响的研究[D].硕士学位论文.南京:南京农业大学,2009.
[22]PARTANEN K,SILJANDER-RASI H,HONKAVAARA M.Effects of finishing diet and preslaughter fasting time on meat quality in crossbred pigs[J].Agricultural and Food Science,2008,16(3):245-258.
[23]LOUREIRO A,AZOIA N G,GOMES A C,et al.Albumin-based nanodevices as drug carriers[J].Current Pharmaceutical Design,2016,22(10):1371-1390.
[24]LI S K,ZHANG Y S,LI M J,et al.Serum albumin,a good indicator of persistent organ failure in acute pancreatitis[J].BM C Gastroenterology,2017,17:59.
[25]CONNER B J.Treating hypoalbuminemia[J].Veterinary Clinics of North America:Small Animal Practice,2017,47(2):451-459.
[26]GERSON A R,GUGLIELMO C G.House sparrows(Passer domesticus)increase protein catabolism in response to w ater restriction[J].American Journal of Physiology-Regulatory,Integrative and Comparative Physiology,2011,300(4):R925-R930.
[27]KNOWLES T G,WARRISS P D,BROWN S N,et al.Response of broilers to deprivation of foodand w ater for 24 hours[J].British Veterinary Journal,1995,151(2):197-202.
[28]HOCKING P M,MAXWELL M H,MITCHELL MA.Haematology and blood composition at tw o ambient temperatures in genetically fat and lean adult broiler breeder females fed ad libitum or restricted throughout life[J].British Poultry Science,1994,35(5):799-807.
[29]KOTT J M,MOONEY-LEBER S M,SHOUBAH FA,et al.Effectiveness of different corticosterone administration methods to elevate corticosterone serum levels,induce depressive-like behavior,and affect neurogenesis levels in female rats[J].Neuroscience,2016,312(3):201-214.
[2]GALLO C B,HUERTAS S M.Main animal welfare problems in ruminant livestock during preslaughter operations:a South American view[J].Animal,2016,10(2):357-364.
[3]张林.运输应激对肉仔鸡肌肉品质的影响及其机理[D].硕士学位论文.杨凌:西北农林科技大学,2009.
[4]BERMEJO-POZA R,DE LA FUENTE J,PREZ C,et al.Reducing the effect of pre-slaughter fasting on the stress response of rainbow trout(Oncorhynchus mykiss)[J].Animal Welfare,2016,25(3):339-346.
[5]KARACA S,ERDO GˇAN S,KOR D,et al.Effects of pre-slaughter diet/management system and fasting period on physiological indicators and meat quality traits of lambs[J].M eat Science,2016,116:67-77.
[6]YANG C,HE J,CHEN D W,et al.Effects of corn type and fasting time before slaughter on grow th and plasma index in w eaning pigs[J].Journal of Animal Science,2016,94(1):106-116.
[7]SMIDT M J,FORMICA S D,FRITZ J C.Effect of fasting prior to slaughter on yield of broilers[J].Poultry Science,1964,43(4):931-934.
[8]VEERKAMP C H.Fasting and yield of broilers[J].Poultry Science,1986,65(7):1299-1304.
[9]ZKAN S,AKBA爦Y,ALTAN,et al.The effect of short-term fasting on performance traits and rectal temperature of broilers during the summer season[J].British Poultry Science,2003,44(1):88-95.
[10]MENCONI A,KUTTAPPAN V A,HERNANDEZ-VELASCO X,et al.Evaluation of a commercially available organic acid product on body w eight loss,carcass yield,and meat quality during preslaughter feed w ithdraw al in broiler chickens:a poultry w elfare and economic perspective[J].Poultry Science,2014,93(2):448-455.
[11]DELEZIE E,SWENNEN Q,BUYSE J,et al.The effect of feed w ithdraw al and crating density in transit on metabolism and meat quality of broilers at slaughter w eight[J].Poultry Science,2007,86(7):1414-1423.
[12]VEERKAMP C H.The influence of fasting and transport on yields of broilers[J].Poultry Science,1978,57(3):634-638.
[13]ALI M S,KANG G H,JOO S T.A review:influences of pre-slaughter stress on poultry meat quality[J].Asian-Australasian Journal of Animal Sciences,2008,21(6):912-916.
[14]BRZSKA F,BULUCHEVSKIJ S,STECKA K,et al.The effects of lactic acid bacteria and mannan oligosaccharide,w ith or w ithout fumaric acid,on chicken performance,slaughter yield and digestive tract microflora[J].Journal of Animal and Feed Sciences,2007,16(2):241-251.
[15]SAKI A A,AZADINIA B,KHOSRAVINIA H A,et al.Effects of pre-slaughter feed w ithdraw al and sex on crop,carcass characteristics and some blood parameters in broiler chicken[J].Journal of Agricultural Technology,2011,7(5):1233-1245.
[16]PEREIRA R E P,MARTINS M R F B,MENDES AA,et al.Effects of pre-slaughter fasting on broiler w elfare,meat quality,and intestinal integrity[J].Revista Brasileira de Ciência Avícola,2013,15(2):119-122.
[17]GOTOH H,NOMURA T,ONO K.Glycogen serves as an energy source that maintains astrocyte cell proliferation in the neonatal telencephalon[J].Journal of Cerebral Blood Flow&M etabolism,2017,37(6):2294-2307.
[18]ADEVA-ANDANY M M,GONZLEZ-LUCN M,DONAPETRY-GARCA C,et al.Glycogen metabolism in humans[J].BBA Clinical,2016,5:85-100.
[19]任晋军.间隙性禁食对肝脏缺血再灌注的保护作用及其机制研究[D].博士学位论文.北京:北京协和医学院,2016.
[20]FLETCHER D L,QIAO M,SMITH D P.The relationship of raw broiler breast meat color and pH to cooked meat color and pH[J].Poultry Science,2000,79(5):784-788.
[21]徐昶.禁食和环境温度对鸡肉宰后僵直过程中能量代谢影响的研究[D].硕士学位论文.南京:南京农业大学,2009.
[22]PARTANEN K,SILJANDER-RASI H,HONKAVAARA M.Effects of finishing diet and preslaughter fasting time on meat quality in crossbred pigs[J].Agricultural and Food Science,2008,16(3):245-258.
[23]LOUREIRO A,AZOIA N G,GOMES A C,et al.Albumin-based nanodevices as drug carriers[J].Current Pharmaceutical Design,2016,22(10):1371-1390.
[24]LI S K,ZHANG Y S,LI M J,et al.Serum albumin,a good indicator of persistent organ failure in acute pancreatitis[J].BM C Gastroenterology,2017,17:59.
[25]CONNER B J.Treating hypoalbuminemia[J].Veterinary Clinics of North America:Small Animal Practice,2017,47(2):451-459.
[26]GERSON A R,GUGLIELMO C G.House sparrows(Passer domesticus)increase protein catabolism in response to w ater restriction[J].American Journal of Physiology-Regulatory,Integrative and Comparative Physiology,2011,300(4):R925-R930.
[27]KNOWLES T G,WARRISS P D,BROWN S N,et al.Response of broilers to deprivation of foodand w ater for 24 hours[J].British Veterinary Journal,1995,151(2):197-202.
[28]HOCKING P M,MAXWELL M H,MITCHELL MA.Haematology and blood composition at tw o ambient temperatures in genetically fat and lean adult broiler breeder females fed ad libitum or restricted throughout life[J].British Poultry Science,1994,35(5):799-807.
[29]KOTT J M,MOONEY-LEBER S M,SHOUBAH FA,et al.Effectiveness of different corticosterone administration methods to elevate corticosterone serum levels,induce depressive-like behavior,and affect neurogenesis levels in female rats[J].Neuroscience,2016,312(3):201-214.