灵武长枣采后低温贮藏期间细胞壁组分的变化
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摘要
以灵武长枣为试材,采用紫外分光光度法,研究了采后低温(0~2℃)贮藏条件2℃下果实细胞壁组分变化的规律,以期为灵武长枣的贮藏保鲜提供参考依据。结果表明:在低温(0~2℃)贮藏条件下,果实硬度呈先上升后下降的趋势,表现出跃变型果实的呼吸特征;离子型果胶(ISP)和水溶型果胶(WSP)含量增加,与果实硬度呈极显著负相关;共价结合型果胶(CSP)、纤维素和半纤维素含量呈先上升后下降,与果实硬度均呈正相关,其中共价结合型果胶(CSP)和纤维素含量与果实硬度呈极显著正相关。
Fruits of half red Zizphus jujube Mill.cv.Lingwuchangzao were used as materials,the ultraviolet spectroph was aimed to discuss changes of cell wall components in Zizphus jujube Mill.cv.Lingwuchangzao fruit at low temperature(0-2 ℃)to provide a theoretical basis for the storage and fresh-keeping of Zizphus jujube Mill.cv.Lingwuchangzao fruit.The results showed that fruit hardness showed first increased and then decreased and the respiratory features of the climacteric fruit were shown,the contents of water-soluble pectin and ionic pectin content of the cell wall were significantly increased,the contents was significant negative correlation with fruit hardness.The contents of covalent bond pectin and cellulose and hemicellulose were showed first increased and then decreased,the contents was positive correlation with fruit hardness,the contents of cellulose and covalent bond pectin was significant positive correlation with fruit hardness among them.
Fruits of half red Zizphus jujube Mill.cv.Lingwuchangzao were used as materials,the ultraviolet spectroph was aimed to discuss changes of cell wall components in Zizphus jujube Mill.cv.Lingwuchangzao fruit at low temperature(0-2 ℃)to provide a theoretical basis for the storage and fresh-keeping of Zizphus jujube Mill.cv.Lingwuchangzao fruit.The results showed that fruit hardness showed first increased and then decreased and the respiratory features of the climacteric fruit were shown,the contents of water-soluble pectin and ionic pectin content of the cell wall were significantly increased,the contents was significant negative correlation with fruit hardness.The contents of covalent bond pectin and cellulose and hemicellulose were showed first increased and then decreased,the contents was positive correlation with fruit hardness,the contents of cellulose and covalent bond pectin was significant positive correlation with fruit hardness among them.
引文
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[15]韩雅珊.食品化学实验指导[M].北京:中国农业大学出版社,1992:32-33.
[16]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:211-212.
[17]BRUMMELL D A,CIN V D,CRISOSTO C H,et al.Cell wall metabolism during maturation,ripening and senescence of peach fruit[J].Jounal of Experimental Botany,2004,55(405):2029-2039.
[18]罗自生.柿果实采后软化过程中细胞壁组分代谢和超微结构的变化[J].植物生理与分子生物学学报,2005,31(6):651-656.
[19]纪淑娟,卜凤雅,周倩,等.冷藏对蓝莓果实细胞壁组分及其降解酶活性的影响[J].食品与发酵工业,2014,40(6):199-204.
[20]KETSA S,DAENGKANIT T.Firmness and activities of polygalacturonase,pectinesterase,β-galactosidase and cellulase in ripening durian harvested at different stages of maturity[J].Sci Hortic,1999,80:181-188.
[21]SEYMOUR G B,GROSS K C.Cell wall disassembly and fruit softening[J].Postharvest News Inform,1996,7(3):45-52.
[2]陆定志,傅家瑞,宋松泉.植物衰老及其调控[M].北京:中国农业出版社,1997:4-5.
[3]YOSHIOKA H,AOBA K,KASHIMURA Y.Molecular weight and degree of methoxylation in cell wall polyuronide during softening in pear and apple fruit[J].American Society for Horticultural Science,1992,117:600-606.
[4]HUBER D J,STRAWBERRY.Fruit softening:The potential roles of polyuronides and hemicelluloses[J].Food Science,1984,49:1310-1315.
[5]WADE N L,KAVANAGH E E,HOCKLEY D G,et al.Relationship between softening and the polyuronides in ripening banana fruit[J].Science of Food and Agriculture,1992,60:61-68.
[6]HUBER D J,DONOGHUE E M.Polyuronides in avocado(Persea americana)and tomato(Lycopersicon esculentum)fruit sexhibit markedly different patterns of molecular weight downshifts during ripening[J].Plant Physiology,1993,102:473-480.
[7]冯美,张宁,张宏宝,等.枸杞采后细胞壁组分及其降解酶活性的变化[J].干旱地区农业研究,2011,29(5):111-114.
[8]魏建梅,马锋旺,关军锋,等.京白梨果实后熟软化过程中细胞壁代谢及其调控[J].中国农业科学,2009,42(8):2987-2996.
[9]NIKOLIC M V,MOJOVIC L.Hydrolysis of apple pectin by the coordinated activity of pectic enzymes[J].Food Chemistry,2007,101:1-9.
[10]LAZAN H,NG S Y,GOH L Y,et al.Papaya B-galactosidase/galactanase is forms in differential cell wall hydrolysis and fruit softening during ripening[J].Plant Physiology and Biochemistry,2004,42:847-853.
[11]田寿乐,周俊义,薛晓敏.冬枣软化衰老过程中细胞壁酶的活性变化[J].食品科学,2007,28(4):220-222.
[12]吴萍,田世平,徐勇.气调贮藏对壶瓶枣果实细胞壁和角质层成分及品质的影响[J].中国农业科学,2009,42(2):619-625.
[13]茅林春.桃果实成熟和絮败的生理学研究[D].杭州:浙江大学,2000.
[14]FISHMAN M L,LEVAJ B,GILLESPIE D.Changes in the physiceo-hemieal properties of peach fruit peetin during on-tree rioenin gand storage[J].Am Soe Hortic Sci,1993,118(3):343-349.
[15]韩雅珊.食品化学实验指导[M].北京:中国农业大学出版社,1992:32-33.
[16]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:211-212.
[17]BRUMMELL D A,CIN V D,CRISOSTO C H,et al.Cell wall metabolism during maturation,ripening and senescence of peach fruit[J].Jounal of Experimental Botany,2004,55(405):2029-2039.
[18]罗自生.柿果实采后软化过程中细胞壁组分代谢和超微结构的变化[J].植物生理与分子生物学学报,2005,31(6):651-656.
[19]纪淑娟,卜凤雅,周倩,等.冷藏对蓝莓果实细胞壁组分及其降解酶活性的影响[J].食品与发酵工业,2014,40(6):199-204.
[20]KETSA S,DAENGKANIT T.Firmness and activities of polygalacturonase,pectinesterase,β-galactosidase and cellulase in ripening durian harvested at different stages of maturity[J].Sci Hortic,1999,80:181-188.
[21]SEYMOUR G B,GROSS K C.Cell wall disassembly and fruit softening[J].Postharvest News Inform,1996,7(3):45-52.