107份小麦历史品种抗穗发芽基因型的检测
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摘要
为了在历史小麦品种中鉴定出抗穗发芽的种质材料,利用与小麦抗穗发芽相关的分子标记Vp1A3和Vp1B3对107份我国小麦历史品种的穗发芽抗性进行综合筛选。结果表明,属于抗穗发芽单倍型Vp-1Agm的材料有3份,分别是郑州742、陕农17、内乡5号;属于抗穗发芽单倍型Vp-1Bb的材料有10份,分别是跃进5号、泰山1号、济南12、泾阳60、复壮30、丰抗15、济农2号、北京8号、济南矮6号、京130;属于抗穗发芽单倍型Vp-1Bc的材料有48份。在具有发芽指数的77份材料中,平均发芽指数小于10%的抗穗发芽基因型材料有5份,分别为安选2号、烟农15、郑州683、郑州742、克群。该研究结果为小麦抗穗发芽育种中抗性亲本的选择提供了基因水平的依据,拓宽了小麦抗穗发芽育种亲本的选择范围。
In order to identify preharvest sprouting(PHS)tolerance of breeding materials,in this study,two STS markers Vp1A3 and Vp1B3associated with PHS tolerance,were used to evaluate the PHS tolerance in 107 Chinese historical wheat cultivars.Three genotypes,Zhengzhou 742,Shannong17 and Neixiang 5,were resistant to PHS haplotype of Vp-1Agm.Ten genotypes,Yuejin 5,Taishan1,Jinan 12,Jingyang 60,Fuzhuang 30,Fengkang 15,Jinong 2,Beijing 8,Jinanai 6and Jing 130,were resistant to PHS haplotype of Vp-1Bb.Forty-eight genotypes were resistant to PHS haplotype of Vp-1Bc.The results showed that the average germination index of five genotypes,such as Anxuan 2,Yannong 15,Zhengzhou 683,Zhengzhou 742 and Kequn,was less than 10%,among 77 genotypes with data of average germination index.These results broaden the scope of parent selection and are useful in wheat improvement for PHS resistance.
In order to identify preharvest sprouting(PHS)tolerance of breeding materials,in this study,two STS markers Vp1A3 and Vp1B3associated with PHS tolerance,were used to evaluate the PHS tolerance in 107 Chinese historical wheat cultivars.Three genotypes,Zhengzhou 742,Shannong17 and Neixiang 5,were resistant to PHS haplotype of Vp-1Agm.Ten genotypes,Yuejin 5,Taishan1,Jinan 12,Jingyang 60,Fuzhuang 30,Fengkang 15,Jinong 2,Beijing 8,Jinanai 6and Jing 130,were resistant to PHS haplotype of Vp-1Bb.Forty-eight genotypes were resistant to PHS haplotype of Vp-1Bc.The results showed that the average germination index of five genotypes,such as Anxuan 2,Yannong 15,Zhengzhou 683,Zhengzhou 742 and Kequn,was less than 10%,among 77 genotypes with data of average germination index.These results broaden the scope of parent selection and are useful in wheat improvement for PHS resistance.
引文
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[12]Yang Y(杨燕),Zhao X L(赵献林),Zhang Y(张勇),et al.Evaluation and validation of four molecular markers associated with pre-harvest sprouting tolerance in Chinese wheats[J].Acta Agronomica Sinica(作物学报),2008,34(1):17-24(in Chinese with English abstract).
[13]Yang Y(杨燕),Zhang C L(张春利),Chen X M(陈新民),et al.Identification and validatation of molecular marker for PHS tolerance in red-grained spring wheat[J].Journal of Triticeae Crops(麦类作物学报),2011,31(1):54-59(in Chinese with English abstract).
[14]Bailey P C,Mckibbin R S.Genetic map location for orthologous VP1 genes in wheat and rice[J].Theoretical and Applied Genetic,1999,98:281-284.
[15]Xia L Q,Gana M W,Shewry P R,et al.Exploiting the diversity of Viviparous1-gene associated with pre-harvest sprouting tolerance in European wheat varieties[J].Euphytica,2008,159(3):411-417.
[16]Xiao S H(肖世和),Yan C S(闫长生),Zhang H P(张海萍),et al.Studies for pre-harvest sprouting of wheat[M].Beijing:China Agricultural Science and Technology Press(北京:中国农业学科出版社),2004:266-292(in Chinese).
[17]McCarty D R,Hattori T.The Viviparous1-developmental gene of maize encodes a novel transcriptional activator[J].Cell,1991,66:895-90.
[2]Xiao S H,Zhang X Y,Yan C S,et al.Germplasm improvement for pre-harvest sprouting resistance in Chinese white-grained wheat:An overview of the current strategy[J].Euphytica,2002,126:35-38.
[3]Appels R,Francki M,Chibbar R.Advances in cereal functional genomics[J].2003,3:1-24.
[4]Kulwal P L,Kumar N,Gaur A,et al.Mapping of a major QTL for pre-harvest sprouting tolerance on chromosome 3A in bread wheat[J].Theoretical and Appllied Genetic,2005,111(6):1052-1059.
[5]Flintham J,Adlam R,Bassoi M,et al.Mapping genes for resistance to sprouting damage in wheat[J].Euphytica,2002,126:39-45.
[6]Roy J K,Prasad M,Varshney R K.Identification of a microsatellite on chromosomes 6Band a STS on 7Dof bread wheat showing an association with pre-harvest sprouting tolerance[J].Theoretical and Applied Genetic,1999,99:336-340.
[7]Mares D,Mrva K,Cheong J,et al.QTL located on chromosome 4Aassociated with dormancy in white-and red-grained wheats of diverse origin[J].Theoretical and Applied Genetic,2005,111:1357-1364.
[8]Yang Y,Zhao X L,Xia L Q,et al.Development and validation of a Viviparous1-STS marker for pre-harvest sprouting resistence in Chinese wheat[J].Theoretical and Applied Genetic,2007 115:971-980.
[9]Yang Y,Zhang C L,Liu S X,et al.Characterization of the rich haplotypes of Viviparous1-Ain Chinese wheats and development of a novel sequence-tagged site marker for pre-harvest sprouting resistance[J].Molecular Breeding,2014,33(1):75-88.
[10]Zhang C L(张春利).Development and validation of functional marker for resistance to pre-harvest sprouting[D].Beijing:Chinese Academy of Agricultural Sciences(北京:中国农业科学院),2009(in Chinese with English abstract).
[11]Yang Y(杨燕),Zhang C L(张春利),Chen X M(陈新民),et al.Identification of wheat genotypes with pre-harvest sprouting tolerance by combinated analysis of spike germination rate,germination index and molecular marker Vp1B3[J].Journal of Triticeae Crops(麦类作物学报),2007,27(4):577-582(in Chinese with English abstract).
[12]Yang Y(杨燕),Zhao X L(赵献林),Zhang Y(张勇),et al.Evaluation and validation of four molecular markers associated with pre-harvest sprouting tolerance in Chinese wheats[J].Acta Agronomica Sinica(作物学报),2008,34(1):17-24(in Chinese with English abstract).
[13]Yang Y(杨燕),Zhang C L(张春利),Chen X M(陈新民),et al.Identification and validatation of molecular marker for PHS tolerance in red-grained spring wheat[J].Journal of Triticeae Crops(麦类作物学报),2011,31(1):54-59(in Chinese with English abstract).
[14]Bailey P C,Mckibbin R S.Genetic map location for orthologous VP1 genes in wheat and rice[J].Theoretical and Applied Genetic,1999,98:281-284.
[15]Xia L Q,Gana M W,Shewry P R,et al.Exploiting the diversity of Viviparous1-gene associated with pre-harvest sprouting tolerance in European wheat varieties[J].Euphytica,2008,159(3):411-417.
[16]Xiao S H(肖世和),Yan C S(闫长生),Zhang H P(张海萍),et al.Studies for pre-harvest sprouting of wheat[M].Beijing:China Agricultural Science and Technology Press(北京:中国农业学科出版社),2004:266-292(in Chinese).
[17]McCarty D R,Hattori T.The Viviparous1-developmental gene of maize encodes a novel transcriptional activator[J].Cell,1991,66:895-90.