悬浮芯片技术在食品安全检测方面应用进展
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摘要
食品安全是重要的民生问题,如何保障食品安全,守护"舌尖上的中国"一直是国家和人民关注的焦点问题。悬浮芯片技术是唯一被美国食品药品管理局(Food and Drug Administration, FDA)批准的生物芯片技术,具有准确、灵敏、高通量等优点,可以满足食品安全部门监管,食品行业自检需求,被越来越多的研究者应用于食品安全方面的检测。因此了解该技术的发展及应用对研究者有重要意义。本文主要对悬浮芯片技术的原理及组成、在食品安全检测中的应用进行了概述,阐述了近5年该技术在检测食源性微生物、农兽药残留、转基因食品和过敏原方面的应用,并对悬浮芯片技术进一步发展方向进行展望。
Food safety is an important problem of people's livelihood. How to protect food safety and protect "A Bite of China" has always been the focus of attention of the country and the people. Suspension chip technology is the only biochip technology approved by the US Food and Drug Administration(FDA). It has the advantages of accuracy, sensitivity and high throughput. It can meet the supervision of food safety departments and the self-test requirements of the food industry. An increasing number of researchers used that for detection in food safety.Therefore, understanding the development and application of this technology is of great significance to researchers.This paper mainly summarized the principle and composition of suspension chip technology in food safety testing,and described the application of this technology in detecting foodborne microorganisms, agricultural and veterinary drug residues, genetically modified foods and allergens in the past 5 years, then looked forward to the further development of the suspension chip technology.
Food safety is an important problem of people's livelihood. How to protect food safety and protect "A Bite of China" has always been the focus of attention of the country and the people. Suspension chip technology is the only biochip technology approved by the US Food and Drug Administration(FDA). It has the advantages of accuracy, sensitivity and high throughput. It can meet the supervision of food safety departments and the self-test requirements of the food industry. An increasing number of researchers used that for detection in food safety.Therefore, understanding the development and application of this technology is of great significance to researchers.This paper mainly summarized the principle and composition of suspension chip technology in food safety testing,and described the application of this technology in detecting foodborne microorganisms, agricultural and veterinary drug residues, genetically modified foods and allergens in the past 5 years, then looked forward to the further development of the suspension chip technology.
引文
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[2]EU-ToxRisk-An Integrated European‘Flagship’Programme Driving Mechanism-based Toxicity Testing and Risk Assessment for the 21st century[Z].http://www.eu-toxrisk.eu/
[3]赵春杰,侯哲,袁丽华,等.气相色谱法同时测定西洋参药材根中12种农药残留的含量[J].沈阳大学学报(自然科学版),2014,(26):193-197.Zhao CJ,Hou Z,Yuan LH,et al.Gas chromatography method for simultaneous determination of twelve pesticide residues in root of rucella ginseng[J].J Shenyang Univ(Nat Sci Ed),2014,(26):193-197.
[4]项林敏,隋玉杰.化学品中29种酯类物质同时检测方法的研究[J].中小企业管理与应用,2018,(1):170-171.Xiang LM,Sui YJ.Research on the simultaneous detection method of 29kinds of esters in cosmetics[J].Manag Technol SEM,2018,(1):170-171.
[5]陈兵,李艳.离子色谱法测定玩具当中可迁移六价铬[J].化工管理,2018,(1):255.Chen B,Li Y.Determination of movable chromium in toys by ion chromatography[J].Chem Manag,2018,(1):255.
[6]Ab Cam ELISA Kits specifications[Z].http://www.abcam.com.
[7]Gu B,Zhang Q.Recent advances on functionalized upconversion nanoparticles for detection of small molecules and ions in rucellaI[J].Adv Sci,2018,(5):1700609-1700616.
[8]Jiang SY,Peng Y,Ning BA,et al.Surface plasmon resonance sensor based on molecularly imprintedpolymer film for detection of histamine[J].Sensor Actuator B,2015,(221):15-21.
[9]Tian JH,Bai JL,Peng Y,et al.Core-shell-structured molecularly imprinted polymer on upconverting nanoparticles for selective and sensitive fluorescent sensing of sulfamethazine[J].Analyst,2015,(140):5520-5526.
[10]Zou Y,Li L.Identification of six serum antigens and autoantibodies for the detection of early stage epithelial ovarian carcinoma by bioinformatics analysis and liquid chip analysis[J].Oncol Lett,2018,(3):1-10.
[11]Vidal M,Aguilar R,CampoJJ,et al.Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple plasmodium falciparum antigens[J].J Immunol Method,2018,(2):1-14.
[12]Aoki K,Tanaka H,Kawahara T.Multiplexed microsphere suspension-array assay for urine mitochondrial DNA typing by C-stretch length in hypervariable regions[J].J Clin Med Res,2018,(10):552-561.
[13]Du Y,Li H,Yin Z,et al.Development of a molecular serotyping scheme and a multiplexed luminex-based array for providencia[J].J Microbiol Method,2018,(153):14-23.
[14]JiangL,Shen Y,Zheng K,et al.Rapid and multiplex microRNA detection on graphically encoded silica suspension array[J].Biosensor Bioelectron,2014,(61):222-226.
[15]Jelsma T,Van DWFJ,Fijten H,et al.Pre-Screening of crude peptides in a serological bead-based suspensionarray[J].J Virolog Method,2017,(247):114-118.
[16]Parsa SF,Vafajoo A,Rostami A,et al.Early diagnosis of disease using microbead array technology:A review[J].Anal Chim Acta,2018,(1032):1-17.
[17]He L,De F,Liang F,et al.Application of suspension array technology for the genetic diagnosis of non-syndromic hearing loss[J].Chin J Med Genet,2018,35(3):351-356.
[18]Zekeridou A,Giannopoulou C,Cancela J,et al.Effect of initial periodontal therapy on gingival crevicular fluid cytokine profile in subjects with chronic periodontitis[J].Clin Exp Dent Res,2017,(3):62-68.
[19]Debnath M,Nagappa M,Talukdar PM,et al.Comprehensive cytokine profiling provides evidence for a multi-lineage the responses in guillainbarrésyndrome[J].Cytokine,2018,(110):58-62.
[20]Liu N,Zheng FJ,Zheng XL.Detection of biomarkers of acute myocardial infarction by high-throughput suspension array technology in serum sample[J].Bioanalysis,2018,(10):47-58.
[21]Yamamura S,Yamada E,Kimura F,et al.Separation and analysis of adherent and non-adherent cancer cells using a single-cell microarray chip[J].Sensors,2017,(17):2410-2411.
[22]Duan K,Ghosh G,Lo JF.Optimizing multiplexed detections of diabetes antibodies via quantitative microfluidic droplet array[J].Small,2017,(13):1702323-1702328.
[23]Stewart A,Banerji U.Utilizing the luminex magnetic bead-based suspension array for rapid multiplexed phosphoprotein quantification[J].Kinas Signal Network,2017,(1636):119-131.
[24]Dunbar SA,Ritchie VB,Hoffmeyer MR,et al.Luminex?multiplex bead suspension arrays for the detection and serotyping of Salmonella spp.[J].Salmonella,2015,(1225):1-27.
[25]Bergqvist C,Holmstr?m P,Lindegren G,et al.Multiplex nucleic acid suspension bead arrays for detection and subtyping of filoviruses[J].JClin Microbiol,2015,(53):1368-1370.
[26]Xu K,Sun Y,Li W,et al.Multiplex chemiluminescent immunoassay for screening of mycotoxins using photonic crystal microsphere suspension array[J].Analyst,2014,(139):771-777.
[27]Zhao J,Kang L,Hu R,et al.Rapid oligonucleotide suspension array-based multiplex detection of bacterial pathogens[J].Foodborne Pathog Dis,2013,(10):896-903.
[28]Wang Y,Ning B,Peng Y,et al.Application of suspension array for simultaneous detection of four different mycotoxins in corn and peanut[J].Biosensor Bioelectron,2013,(41):391-396.
[29]Liu N,Gao ZX,Ma HW,et al.Simultaneous and rapid detection of multiple pesticide and veterinary drug residues by suspension array technology[J].Biosens Bioelectron,2013,(41):710-716.
[30]苏璞.牛奶中多种抗生素的悬浮芯片检测技术研究[D].北京:中国人民解放军军事医学科学院,2011.Su P.Suspension array technology for simultaneous detection of multiple antibiotics residues in milk[D].Beijing:Academy of Military Medical Sciences of the Chinese PLA,2011.
[31]Sun ZY,Peng Y,Zhang MC.et al.Simultaneous and highly sensitive detection of six different foodbornepathogens by high-throughput suspension array technology[J].Food Contr,2014,(11):300-309
[32]Leng Y,Sun K,Chen X,et al.Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection[J].Chem Soc Rev,2015,(1):1-44.
[33]Dunbar SA,Li D.Introduction to luminex xmap technology and applications for biological analysis in china[Z].2010,(1):1-5.
[34]Yang S,Li W,Ye C,et al.C 3N-a 2D crystalline,hole-free,tunable-narrow-bandgap semiconductor with ferromagnetic properties[J].Adv Mater,2017,(29):1605625-1605627.
[35]Xie M,Hu J,Wen CY,et al.Fluorescent-magnetic dual-encoded nanospheres:A promising tool for fast-simultaneous-addressable high-throughput analysis[J].Nanotechnology,2011,(23):2-13.
[36]Zhong Y,Tian G,Gu Z,et al.Elimination of photon quenching by a transition layer to fabricate a quenching-shield sandwich structure for 800nm excited upconversion luminescence of Nd3+-sensitized nanoparticles[J].AdvMater,2013,(26):2831-2837.
[37]Fan Y,Wang P,Lu Y,et al.Lifetime-engineered NIR-II nanoparticles unlock multiplexed in vivo imaging[J].Nat Nanotechnol,2018,(5):1-11.
[38]Zhou L,Fan Y,Wang R,et al.High-capacity upconversion wavelength and lifetime binary encoding for multiplexed biodetection[J].Angew Chem Int Ed,2018,(52):3584-3587.
[39]杨子学,陈宝安,顾忠泽.光子晶体编码液相芯片技术与肿瘤筛查[J].中国肿瘤临床,2014,(41):42-45.Yang ZX,Chen BA,Gu ZZ.Photonic crystal-encoded suspension array and its application in screening malignant tumors[J].Chin J Clin Oncol,2014,(41):42-45.
[40]Zhang B,Cai Y,Shang L,et al.A photonic crystal hydrogel suspension array for the capture of blood cells from whole blood[J].Nanoscale,2016,(8):3841-3847.
[41]Luan C,Xu Y,Professor BC,et al.Responsive photonic encoded breathing microbeads based microfluidic chip for multiplex fluorescent immunoassay[J].Sensor ActuatorB:Chem,2017,(242):1259-1264.
[42]Shang L,Fu F,Cheng Y,et al.Photonic crystal microbubbles as suspension barcodes[J].Jam Chem Soc,2015,(137):15533-15539.
[43]Lu Y,Zhao J,Zhang R,et al.Tunable lifetime multiplexing using luminescent nanocrystals[J].Nat Photonic,2018,(8):32-36.
[44]Gorpas D,Ma D,Bec J,et al.Real-time visualization of tissue surface biochemical features derived from fluorescence lifetime measurements[J].IEEE Trans Med Imag,2016,(35):1802-1811.
[45]Yang MY,Zhang M,Cui Y,et al.A smartphone-based quantitative detection platform of mycotoxins based on multiple-color upconversion nanoparticles[J].Nanoscale,2018,(10):15865-15874.
[46]You M,Lin M,Gong Y,et al.Household fluorescent lateral flow strip platform for sensitive and quantitative prognosis of heart failure using dual-color upconversion nanoparticles[J].ACS Nano,2017,(11):6261-6270.
[47]Vander WFJ,Achterberg RP,Maassen CBM.A bead-based suspension array for the detection of salmonella antibodies in pig serac[J].BMC Vet Res,2018,(14):226.
[48]Aydin M,Carter-Conger J,Gao N,et al.Molecular identification of common Salmonellaserovars using multiplex DNA sensor-based suspension array[J].Anal Bioanal Chem,2018,(410):2637.
[49]Yu X,Torzewska A,Zhang X,et al.Genetic diversity of the o antigens of proteus species and the development of a suspension array for molecular serotyping[J].PLoS One,2017,(12):267-276.
[50]Kase JA,Maounounen-Laasri A,Lin A.Rapid identification of shiga toxin-producing rucellaia coli O serogroups from fresh produce and raw milk enrichment cultures by luminex bead-based suspension array[J].JFood Protect,2016,(79):1623-1629.
[51]Carter JM,Lin A,Clotilde L,et al.Rapid,multiplexed characterization of shiga toxin-producing Escherichia Coli(STEC)isolates using suspension array technology[J].Front Microbiol,2016,(7):373-376.
[52]Pfefer TSL,Timme R,Kase JA.Identification of rucella genus and eight rucella species by luminex bead-based suspension array[J].Food Microbiol,2017,(1):1-44.
[53]Zhang X,Xie F,Lv B,et al.Suspension array for multiplex detection of eight fungicide-resistance related alleles in botrytis cinerea[J].Front Microbiol,2016,(7):4632-4710.
[54]Van Brunschot SL,Bergervoet JHW.A bead-based suspension array for the multiplexed detection of begomoviruses and their whitefly vectors[J].J Virolog Method,2014,(198):86-94.
[55]Vander Wal FJ,Jelsma T,Fijten H,et al.Towards a peptide-based suspension array for the detection of pestivirus antibodies in Swine[J].JVirolog Method,2016,(2):1-13.
[56]Wang X,Mu Z,Shangguan F,et al.Simultaneous detection of fenitrothion and chlorpyrifos-methyl with a photonic suspension array[J].PLoS One,2013,(8):e66703-e66707.
[57]Wang X,Mu Z,Shangguan F,et al.Rapid and sensitive suspension array for multiplex detection of organophosphorus pesticides and carbamate pesticides based on silica-hydrogel hybrid microbeads[J].J Hazardous Mater,2014,(273):287-292.
[58]Han X,Wang H,Chen H,et al.Development and primary application of a fluorescent liquid bead array for the simultaneous identification of multiple genetically modified maize[J].Biosens Bioelectron,2013,(49):360-366.
[59]Christopoulou S,Karaiskou S,Kalogianni DP.Microbead-based simultaneous fluorometric detection of three nut allergens[J].Microchim Acta,2017,(3):1-8.
[60]Li CY,Cao D,Qi CB,et al.Combining holographic optical tweezers with upconversion luminescence encoding:imaging-based stable suspension array for sensitive responding of dual cancer biomarkers[J].Anal Chem,2018,(90):2639-2647.
[61]Yuan C,Deng YT,Li XM,et al.Synthesis of monodisperse plasmonic magnetic microbeads and their application in ultrasensitive detection of biomolecules[J].Anal Chem,2018,(13):8178-8187.
[2]EU-ToxRisk-An Integrated European‘Flagship’Programme Driving Mechanism-based Toxicity Testing and Risk Assessment for the 21st century[Z].http://www.eu-toxrisk.eu/
[3]赵春杰,侯哲,袁丽华,等.气相色谱法同时测定西洋参药材根中12种农药残留的含量[J].沈阳大学学报(自然科学版),2014,(26):193-197.Zhao CJ,Hou Z,Yuan LH,et al.Gas chromatography method for simultaneous determination of twelve pesticide residues in root of rucella ginseng[J].J Shenyang Univ(Nat Sci Ed),2014,(26):193-197.
[4]项林敏,隋玉杰.化学品中29种酯类物质同时检测方法的研究[J].中小企业管理与应用,2018,(1):170-171.Xiang LM,Sui YJ.Research on the simultaneous detection method of 29kinds of esters in cosmetics[J].Manag Technol SEM,2018,(1):170-171.
[5]陈兵,李艳.离子色谱法测定玩具当中可迁移六价铬[J].化工管理,2018,(1):255.Chen B,Li Y.Determination of movable chromium in toys by ion chromatography[J].Chem Manag,2018,(1):255.
[6]Ab Cam ELISA Kits specifications[Z].http://www.abcam.com.
[7]Gu B,Zhang Q.Recent advances on functionalized upconversion nanoparticles for detection of small molecules and ions in rucellaI[J].Adv Sci,2018,(5):1700609-1700616.
[8]Jiang SY,Peng Y,Ning BA,et al.Surface plasmon resonance sensor based on molecularly imprintedpolymer film for detection of histamine[J].Sensor Actuator B,2015,(221):15-21.
[9]Tian JH,Bai JL,Peng Y,et al.Core-shell-structured molecularly imprinted polymer on upconverting nanoparticles for selective and sensitive fluorescent sensing of sulfamethazine[J].Analyst,2015,(140):5520-5526.
[10]Zou Y,Li L.Identification of six serum antigens and autoantibodies for the detection of early stage epithelial ovarian carcinoma by bioinformatics analysis and liquid chip analysis[J].Oncol Lett,2018,(3):1-10.
[11]Vidal M,Aguilar R,CampoJJ,et al.Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple plasmodium falciparum antigens[J].J Immunol Method,2018,(2):1-14.
[12]Aoki K,Tanaka H,Kawahara T.Multiplexed microsphere suspension-array assay for urine mitochondrial DNA typing by C-stretch length in hypervariable regions[J].J Clin Med Res,2018,(10):552-561.
[13]Du Y,Li H,Yin Z,et al.Development of a molecular serotyping scheme and a multiplexed luminex-based array for providencia[J].J Microbiol Method,2018,(153):14-23.
[14]JiangL,Shen Y,Zheng K,et al.Rapid and multiplex microRNA detection on graphically encoded silica suspension array[J].Biosensor Bioelectron,2014,(61):222-226.
[15]Jelsma T,Van DWFJ,Fijten H,et al.Pre-Screening of crude peptides in a serological bead-based suspensionarray[J].J Virolog Method,2017,(247):114-118.
[16]Parsa SF,Vafajoo A,Rostami A,et al.Early diagnosis of disease using microbead array technology:A review[J].Anal Chim Acta,2018,(1032):1-17.
[17]He L,De F,Liang F,et al.Application of suspension array technology for the genetic diagnosis of non-syndromic hearing loss[J].Chin J Med Genet,2018,35(3):351-356.
[18]Zekeridou A,Giannopoulou C,Cancela J,et al.Effect of initial periodontal therapy on gingival crevicular fluid cytokine profile in subjects with chronic periodontitis[J].Clin Exp Dent Res,2017,(3):62-68.
[19]Debnath M,Nagappa M,Talukdar PM,et al.Comprehensive cytokine profiling provides evidence for a multi-lineage the responses in guillainbarrésyndrome[J].Cytokine,2018,(110):58-62.
[20]Liu N,Zheng FJ,Zheng XL.Detection of biomarkers of acute myocardial infarction by high-throughput suspension array technology in serum sample[J].Bioanalysis,2018,(10):47-58.
[21]Yamamura S,Yamada E,Kimura F,et al.Separation and analysis of adherent and non-adherent cancer cells using a single-cell microarray chip[J].Sensors,2017,(17):2410-2411.
[22]Duan K,Ghosh G,Lo JF.Optimizing multiplexed detections of diabetes antibodies via quantitative microfluidic droplet array[J].Small,2017,(13):1702323-1702328.
[23]Stewart A,Banerji U.Utilizing the luminex magnetic bead-based suspension array for rapid multiplexed phosphoprotein quantification[J].Kinas Signal Network,2017,(1636):119-131.
[24]Dunbar SA,Ritchie VB,Hoffmeyer MR,et al.Luminex?multiplex bead suspension arrays for the detection and serotyping of Salmonella spp.[J].Salmonella,2015,(1225):1-27.
[25]Bergqvist C,Holmstr?m P,Lindegren G,et al.Multiplex nucleic acid suspension bead arrays for detection and subtyping of filoviruses[J].JClin Microbiol,2015,(53):1368-1370.
[26]Xu K,Sun Y,Li W,et al.Multiplex chemiluminescent immunoassay for screening of mycotoxins using photonic crystal microsphere suspension array[J].Analyst,2014,(139):771-777.
[27]Zhao J,Kang L,Hu R,et al.Rapid oligonucleotide suspension array-based multiplex detection of bacterial pathogens[J].Foodborne Pathog Dis,2013,(10):896-903.
[28]Wang Y,Ning B,Peng Y,et al.Application of suspension array for simultaneous detection of four different mycotoxins in corn and peanut[J].Biosensor Bioelectron,2013,(41):391-396.
[29]Liu N,Gao ZX,Ma HW,et al.Simultaneous and rapid detection of multiple pesticide and veterinary drug residues by suspension array technology[J].Biosens Bioelectron,2013,(41):710-716.
[30]苏璞.牛奶中多种抗生素的悬浮芯片检测技术研究[D].北京:中国人民解放军军事医学科学院,2011.Su P.Suspension array technology for simultaneous detection of multiple antibiotics residues in milk[D].Beijing:Academy of Military Medical Sciences of the Chinese PLA,2011.
[31]Sun ZY,Peng Y,Zhang MC.et al.Simultaneous and highly sensitive detection of six different foodbornepathogens by high-throughput suspension array technology[J].Food Contr,2014,(11):300-309
[32]Leng Y,Sun K,Chen X,et al.Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection[J].Chem Soc Rev,2015,(1):1-44.
[33]Dunbar SA,Li D.Introduction to luminex xmap technology and applications for biological analysis in china[Z].2010,(1):1-5.
[34]Yang S,Li W,Ye C,et al.C 3N-a 2D crystalline,hole-free,tunable-narrow-bandgap semiconductor with ferromagnetic properties[J].Adv Mater,2017,(29):1605625-1605627.
[35]Xie M,Hu J,Wen CY,et al.Fluorescent-magnetic dual-encoded nanospheres:A promising tool for fast-simultaneous-addressable high-throughput analysis[J].Nanotechnology,2011,(23):2-13.
[36]Zhong Y,Tian G,Gu Z,et al.Elimination of photon quenching by a transition layer to fabricate a quenching-shield sandwich structure for 800nm excited upconversion luminescence of Nd3+-sensitized nanoparticles[J].AdvMater,2013,(26):2831-2837.
[37]Fan Y,Wang P,Lu Y,et al.Lifetime-engineered NIR-II nanoparticles unlock multiplexed in vivo imaging[J].Nat Nanotechnol,2018,(5):1-11.
[38]Zhou L,Fan Y,Wang R,et al.High-capacity upconversion wavelength and lifetime binary encoding for multiplexed biodetection[J].Angew Chem Int Ed,2018,(52):3584-3587.
[39]杨子学,陈宝安,顾忠泽.光子晶体编码液相芯片技术与肿瘤筛查[J].中国肿瘤临床,2014,(41):42-45.Yang ZX,Chen BA,Gu ZZ.Photonic crystal-encoded suspension array and its application in screening malignant tumors[J].Chin J Clin Oncol,2014,(41):42-45.
[40]Zhang B,Cai Y,Shang L,et al.A photonic crystal hydrogel suspension array for the capture of blood cells from whole blood[J].Nanoscale,2016,(8):3841-3847.
[41]Luan C,Xu Y,Professor BC,et al.Responsive photonic encoded breathing microbeads based microfluidic chip for multiplex fluorescent immunoassay[J].Sensor ActuatorB:Chem,2017,(242):1259-1264.
[42]Shang L,Fu F,Cheng Y,et al.Photonic crystal microbubbles as suspension barcodes[J].Jam Chem Soc,2015,(137):15533-15539.
[43]Lu Y,Zhao J,Zhang R,et al.Tunable lifetime multiplexing using luminescent nanocrystals[J].Nat Photonic,2018,(8):32-36.
[44]Gorpas D,Ma D,Bec J,et al.Real-time visualization of tissue surface biochemical features derived from fluorescence lifetime measurements[J].IEEE Trans Med Imag,2016,(35):1802-1811.
[45]Yang MY,Zhang M,Cui Y,et al.A smartphone-based quantitative detection platform of mycotoxins based on multiple-color upconversion nanoparticles[J].Nanoscale,2018,(10):15865-15874.
[46]You M,Lin M,Gong Y,et al.Household fluorescent lateral flow strip platform for sensitive and quantitative prognosis of heart failure using dual-color upconversion nanoparticles[J].ACS Nano,2017,(11):6261-6270.
[47]Vander WFJ,Achterberg RP,Maassen CBM.A bead-based suspension array for the detection of salmonella antibodies in pig serac[J].BMC Vet Res,2018,(14):226.
[48]Aydin M,Carter-Conger J,Gao N,et al.Molecular identification of common Salmonellaserovars using multiplex DNA sensor-based suspension array[J].Anal Bioanal Chem,2018,(410):2637.
[49]Yu X,Torzewska A,Zhang X,et al.Genetic diversity of the o antigens of proteus species and the development of a suspension array for molecular serotyping[J].PLoS One,2017,(12):267-276.
[50]Kase JA,Maounounen-Laasri A,Lin A.Rapid identification of shiga toxin-producing rucellaia coli O serogroups from fresh produce and raw milk enrichment cultures by luminex bead-based suspension array[J].JFood Protect,2016,(79):1623-1629.
[51]Carter JM,Lin A,Clotilde L,et al.Rapid,multiplexed characterization of shiga toxin-producing Escherichia Coli(STEC)isolates using suspension array technology[J].Front Microbiol,2016,(7):373-376.
[52]Pfefer TSL,Timme R,Kase JA.Identification of rucella genus and eight rucella species by luminex bead-based suspension array[J].Food Microbiol,2017,(1):1-44.
[53]Zhang X,Xie F,Lv B,et al.Suspension array for multiplex detection of eight fungicide-resistance related alleles in botrytis cinerea[J].Front Microbiol,2016,(7):4632-4710.
[54]Van Brunschot SL,Bergervoet JHW.A bead-based suspension array for the multiplexed detection of begomoviruses and their whitefly vectors[J].J Virolog Method,2014,(198):86-94.
[55]Vander Wal FJ,Jelsma T,Fijten H,et al.Towards a peptide-based suspension array for the detection of pestivirus antibodies in Swine[J].JVirolog Method,2016,(2):1-13.
[56]Wang X,Mu Z,Shangguan F,et al.Simultaneous detection of fenitrothion and chlorpyrifos-methyl with a photonic suspension array[J].PLoS One,2013,(8):e66703-e66707.
[57]Wang X,Mu Z,Shangguan F,et al.Rapid and sensitive suspension array for multiplex detection of organophosphorus pesticides and carbamate pesticides based on silica-hydrogel hybrid microbeads[J].J Hazardous Mater,2014,(273):287-292.
[58]Han X,Wang H,Chen H,et al.Development and primary application of a fluorescent liquid bead array for the simultaneous identification of multiple genetically modified maize[J].Biosens Bioelectron,2013,(49):360-366.
[59]Christopoulou S,Karaiskou S,Kalogianni DP.Microbead-based simultaneous fluorometric detection of three nut allergens[J].Microchim Acta,2017,(3):1-8.
[60]Li CY,Cao D,Qi CB,et al.Combining holographic optical tweezers with upconversion luminescence encoding:imaging-based stable suspension array for sensitive responding of dual cancer biomarkers[J].Anal Chem,2018,(90):2639-2647.
[61]Yuan C,Deng YT,Li XM,et al.Synthesis of monodisperse plasmonic magnetic microbeads and their application in ultrasensitive detection of biomolecules[J].Anal Chem,2018,(13):8178-8187.