摘要
目的研究结直肠癌细胞炎症模型中基因表达的变化以及所涉及的信号通路。方法利用脂多糖(LPS)刺激SW480细胞1、3、6小时后提取RNA构建测序文库进行转录组测序,对表达有变化的基因归类,并进行京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)分析,对分析结果进行实时荧光定量PCR验证。结果发现LPS刺激SW480细胞1、3、6小时后,3个时间点表达均有显著变化的基因有250个。对这些差异表达的基因进行了KEGG信号通路富集分析发现利用脂多糖刺激构建的炎症模型里,TNF-α激活和NF-κB信号通路活化最显著。利用癌症基因组图谱(TCGA)数据库进一步对表达持续上调或下调的基因进行分析发现,部分基因在结肠癌标本中的表达趋势与LPS刺激结肠癌细胞引起的表达变化趋势一致。结论在利用脂多糖刺激构建的结肠癌细胞炎症模型中,炎症过程中的基因表达变化可能与结肠癌的发生发展密切相关。
Objective To investigate gene expression and involved signaling pathway of inflammation model of colorectal cell line. Methods Lipopolysaccharide(LPS) is used to stimulate sw480 cell line at 1, 3,and 6 hours. Total RNA from the sw480 cells was extracted after LPS exposure and then sequenced to construct library. The sequence data was classified and analyzed using KEGG database. Then, Quantitative Real-time PCR(q RT-PCR) was used to verify the analysis result. Results 250 m RNA transcripts with significant alteration at three time points were collected. The signaling pathways of TNF-α and NF-κB were obviously activated in the inflammation model of sw480 cell induced by LPS through KEGG Pathway enrichment analysis. Some of these significantly altered genes showed the same change tendency between our sequence data and The Cancer Genome Atlas database. Conclusions These changes of gene expression in the inflammation model induced by LPS might participate in the occurrence and development of colorectal cancer.
引文
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