基于硅胶载体表面分子印迹聚合物对牛血清蛋白识别性能
|
摘要
以硅胶作为载体,丙烯酰胺和2-丙烯酰胺基-2-甲基丙磺酸为双功能单体,牛血清蛋白质作为模板分子,制备了牛血清蛋白表面分子印迹聚合物,并对其制备方法、性状和性质进行了检测。结果证明制备的分子印迹聚合物大小在400 nm左右,当功能单体的配比为5∶1时为最优配比,分子印迹聚合物对牛血清蛋白质的结合在60 min时达到平衡,并且在浓度为0. 6mg·m L~(-1)的情况下,结合率达到饱和。特异性检测可知分子印迹聚合物对牛血清蛋白的结合率最高并且其印迹因子(IF)高达1. 58,说明了它对目标蛋白具有良好的选择识别能力,且重复性能好,重复检测后吸附量在80%左右。
Research on bovine serum albumi( BSA) recognition performance based on silica gel surface imprinting polymer,the acrylamide( AAm) and 2-acrylamido-2-methylpropanesulfonic acid( AMPS) were acted as bifunctional monomers. The detection of results show that the size of molecular imprinting polymer( MIP) is 400 nm and the optimum proportion of monomers is 5∶ 1( AAm∶ AMPS). The adsorption process of MIP can reach the adsorption equilibrium at 60 minutes,and the adsorption amount can reach the maximum at the concentration of 0. 6 mg·mL~(-1). The specificity detection showed that the adsorption amount of BSA protein is the highest and the imprinting factor( IF) is as high as 1. 58,indicating that it is good for the target protein. The repeatability detection is very good,and the adsorption rate of MIP is still as high as about 80%.
Research on bovine serum albumi( BSA) recognition performance based on silica gel surface imprinting polymer,the acrylamide( AAm) and 2-acrylamido-2-methylpropanesulfonic acid( AMPS) were acted as bifunctional monomers. The detection of results show that the size of molecular imprinting polymer( MIP) is 400 nm and the optimum proportion of monomers is 5∶ 1( AAm∶ AMPS). The adsorption process of MIP can reach the adsorption equilibrium at 60 minutes,and the adsorption amount can reach the maximum at the concentration of 0. 6 mg·mL~(-1). The specificity detection showed that the adsorption amount of BSA protein is the highest and the imprinting factor( IF) is as high as 1. 58,indicating that it is good for the target protein. The repeatability detection is very good,and the adsorption rate of MIP is still as high as about 80%.
引文
1高婷,郭敏杰,樊志,等.牛血清白蛋白分子印迹壳聚糖树脂的制备[J].天津科技大学学报,2010,25(l):20-23Gao Ting,Guo Minjie,Fan Zhi,et al. Molecular imprinting polymer made of chitosan for BSA[J]. Journal of Tianjin University of Science and Technology,2010,25(1):20-23
2 宋锡瑾,龚伟,王杰.蛋白质分子印迹[J].化学通报,2005,(7):504-509Song Xijin Gong Wei,Wang Jie. Proteins molecular imprinted technique[J],Chemistry,2005,(7):540-509
3 董祥芝,蒋崇珊,孙田将,等.蛋白质分子印迹聚合物的研究进展及应用[J].山东化工,2017,46(17):53-54Dong Xiangzhi,Jiang Chongshan,Sun Tianjing,et al. Development and applications of protein molecularly imprinted polymers[J]. Shandong Chemical Industry,2017,46(17):53-54
4 赵晨,贾光锋,陆文总,等.罗丹明B表面分子印迹聚合物制备及其荧光检测[J].食品科学,2014,35(20):236-241Zhao Chen,Jia Guangfeng,Lu Wenzong,et al. Preparation and fluorescence microscopic characterization of surface molecularly imprinted polymer of Rhoda mine B[J]. Food Science,2014,35(20):236-241
5 赵晨,贾光锋,陆文总,等.表面分子印迹法制备棉酚印迹聚合物及其性能检测[J].科学技术与工程,2014,14(16):88-93Zhao Chen,Jia Guangfeng,Lu Wenzong,et al. Preparation of gossypol surface molecular imprinting polymer and detection[J]. Science Technology and Engineering,2014,14(16):88-93
6 赵晨,胡莉莉,郑三婷.硝酸铵水凝胶分子印迹聚合物制备[J],现代生物医学进展,2011,11(5):864-866Zhao Chen,Hu Lili,Zheng Santing. Synthesis of ammonium nitrate molecularly imprinted polymer within hydrogels[J]. Progress in Modern Biomedicine,2011,11(5):864-866
7 Davoodi D,Hassanzadeh-Khayyat M,Rezaei M,et al. Preparation,evaluation and application of diazinon imprinted polymers as the sorbent in molecularly imprinted solid-phase extraction and liquid chromatography analysis in cucumber and aqueous samples[J]. Food Chemistry,2014,158(1):421-428
8 赵晨,任郁苗,陆文总,等.压电磁性表面分子印迹传感器对氯霉素的检测[J].科学技术与工程,2016,16(15):149-153Zhao Chen,Ren Yumiao,Lu Wenzong,et al. Detection of piezoelectric magnetic surface molecularly imprinted sensor to chloramphenicol[J]. Science Technology and Engineering,2016,16(15):149-153
9 赵晨,贾光锋,陆文总,等.磁性表面分子印迹聚合物对食品中苏丹Ⅰ红的检测[J],现代食品科技,2016,32(9):302-307Zhao Chen,Jia Guangfeng,Lu Wenzong,et al. Detection of SudanⅠin food using magnetic molecularly imprinted polymer microspheres[J]. Modern Food Science and Technology,2016,32(9):302-307
10 孙寅静,罗文卿,潘俊.蛋白质分子印迹技术的研究进展及应用前景[J],药学学报,2011,46(2):132-137Sun Yinjing,Luo Wenqing,Pan Jun. Recent advances and perspective in the study of the molecular imprinting of proteins[J]. Acta Pharmaccutica Sinica,2011,46(2):132-137
11 骆薇,张迎庆.多糖基质蛋白质分子印迹聚合物研究进展[J].中国生化药物杂志,2015,35(2):177-180Luo Wei, Zhang Yingqing. Reserch progress of polysaccharidebased protein molecular imprinting polymer[J]. Chinese Journal of Biochemical and Pharmaceuticals,2015,35(2):177-180
12 林红艳,曾珊珊,王燕梅,等.溶菌酶与羧甲基纤维素层层自组装印迹聚合物[J].武汉理工大学学报,2013,35(2):48-52Lin Hongyan,Zeng Shanshan,Wang Yanmei,et al. Preparation of protein imprinted polymer by layer-layer assembly of lysozyme and carboxymethyl cellulose[J]. Journal of Wuhan University of Technology,2013,35(2):48-52
2 宋锡瑾,龚伟,王杰.蛋白质分子印迹[J].化学通报,2005,(7):504-509Song Xijin Gong Wei,Wang Jie. Proteins molecular imprinted technique[J],Chemistry,2005,(7):540-509
3 董祥芝,蒋崇珊,孙田将,等.蛋白质分子印迹聚合物的研究进展及应用[J].山东化工,2017,46(17):53-54Dong Xiangzhi,Jiang Chongshan,Sun Tianjing,et al. Development and applications of protein molecularly imprinted polymers[J]. Shandong Chemical Industry,2017,46(17):53-54
4 赵晨,贾光锋,陆文总,等.罗丹明B表面分子印迹聚合物制备及其荧光检测[J].食品科学,2014,35(20):236-241Zhao Chen,Jia Guangfeng,Lu Wenzong,et al. Preparation and fluorescence microscopic characterization of surface molecularly imprinted polymer of Rhoda mine B[J]. Food Science,2014,35(20):236-241
5 赵晨,贾光锋,陆文总,等.表面分子印迹法制备棉酚印迹聚合物及其性能检测[J].科学技术与工程,2014,14(16):88-93Zhao Chen,Jia Guangfeng,Lu Wenzong,et al. Preparation of gossypol surface molecular imprinting polymer and detection[J]. Science Technology and Engineering,2014,14(16):88-93
6 赵晨,胡莉莉,郑三婷.硝酸铵水凝胶分子印迹聚合物制备[J],现代生物医学进展,2011,11(5):864-866Zhao Chen,Hu Lili,Zheng Santing. Synthesis of ammonium nitrate molecularly imprinted polymer within hydrogels[J]. Progress in Modern Biomedicine,2011,11(5):864-866
7 Davoodi D,Hassanzadeh-Khayyat M,Rezaei M,et al. Preparation,evaluation and application of diazinon imprinted polymers as the sorbent in molecularly imprinted solid-phase extraction and liquid chromatography analysis in cucumber and aqueous samples[J]. Food Chemistry,2014,158(1):421-428
8 赵晨,任郁苗,陆文总,等.压电磁性表面分子印迹传感器对氯霉素的检测[J].科学技术与工程,2016,16(15):149-153Zhao Chen,Ren Yumiao,Lu Wenzong,et al. Detection of piezoelectric magnetic surface molecularly imprinted sensor to chloramphenicol[J]. Science Technology and Engineering,2016,16(15):149-153
9 赵晨,贾光锋,陆文总,等.磁性表面分子印迹聚合物对食品中苏丹Ⅰ红的检测[J],现代食品科技,2016,32(9):302-307Zhao Chen,Jia Guangfeng,Lu Wenzong,et al. Detection of SudanⅠin food using magnetic molecularly imprinted polymer microspheres[J]. Modern Food Science and Technology,2016,32(9):302-307
10 孙寅静,罗文卿,潘俊.蛋白质分子印迹技术的研究进展及应用前景[J],药学学报,2011,46(2):132-137Sun Yinjing,Luo Wenqing,Pan Jun. Recent advances and perspective in the study of the molecular imprinting of proteins[J]. Acta Pharmaccutica Sinica,2011,46(2):132-137
11 骆薇,张迎庆.多糖基质蛋白质分子印迹聚合物研究进展[J].中国生化药物杂志,2015,35(2):177-180Luo Wei, Zhang Yingqing. Reserch progress of polysaccharidebased protein molecular imprinting polymer[J]. Chinese Journal of Biochemical and Pharmaceuticals,2015,35(2):177-180
12 林红艳,曾珊珊,王燕梅,等.溶菌酶与羧甲基纤维素层层自组装印迹聚合物[J].武汉理工大学学报,2013,35(2):48-52Lin Hongyan,Zeng Shanshan,Wang Yanmei,et al. Preparation of protein imprinted polymer by layer-layer assembly of lysozyme and carboxymethyl cellulose[J]. Journal of Wuhan University of Technology,2013,35(2):48-52