外加碳源对菌株GH10降解BDE-209的影响
|
摘要
通过研究6种外加碳源对菌株GH10降解BDE-209产生的影响,证明葡萄糖是菌GH10降解BDE-209的最佳外加碳源,降解培养基中葡萄糖的最适浓度为2 mg/L。经过5 d的降解培养,在第3天时培养基中BDE-209的残余浓度最低,其浓度从10 mg/L降为1.24 mg/L;与不添加葡萄糖的试验相比,BDE-209的降解率从42.24%提高到87.61%;菌株GH10对BDE-209的降解符合一级反应动力学,降解动力学方程为C=12.064e-0.670 t,半衰期为1.03 d,说明添加葡萄糖能够有效促进菌GH10降解BDE-209。
The effects of six additional carbon sources on the residual concentration of BDE-209 degraded by Strain GH10 were studied. The experimental results showed that the best choice was glucose of which the concentration was 2 mg/L.BDE-209 was degraded by Strain GH10 in the medium with glucose for 5 days,and on the third day the residual concentration of BDE-209 was the lowest as 1.24 mg/L down from 10 mg/L. The degradation rate of BDE-209 was 87.61%,raised from 42.24% compared with the experiment without glucose. The degradation of BDE-209 by Strain GH10 in the medium with glucose follows the first order reaction kinetic model,with kinetic equation as C=12.064e-0.670 tand the half-life of BDE-209 as 1.03 days,which have showed that glucose could effectively promote Strain GH10 to degrade BDE-209.
The effects of six additional carbon sources on the residual concentration of BDE-209 degraded by Strain GH10 were studied. The experimental results showed that the best choice was glucose of which the concentration was 2 mg/L.BDE-209 was degraded by Strain GH10 in the medium with glucose for 5 days,and on the third day the residual concentration of BDE-209 was the lowest as 1.24 mg/L down from 10 mg/L. The degradation rate of BDE-209 was 87.61%,raised from 42.24% compared with the experiment without glucose. The degradation of BDE-209 by Strain GH10 in the medium with glucose follows the first order reaction kinetic model,with kinetic equation as C=12.064e-0.670 tand the half-life of BDE-209 as 1.03 days,which have showed that glucose could effectively promote Strain GH10 to degrade BDE-209.
引文
[1]Harrad S,Diamond M.New directions:exposure to polybrominated diphenyl ethers(PBDEs)and polychlorinated biphenyls(PCBs):current and future scenarios[J].Atmospheric Environment,2006(40):1187-1188.
[2]刘汉霞,张庆华,江桂斌.多溴联苯醚及其环境问题[J].化学进展,2005,17(3):554-561.Liu Hanxia,Zhang Qinghua,Jiang Guibin.Polybrominated diphenyl ethers and its related environmental problems[J].Progress Chemistry,2005,17(3):554-561.(in Chinese)
[3]Bi X,Thomas G O,Jones K C,et a1.Exposure of electronics dismantling workers to polybrominated diphenyl ethers,polychlorinated biphenyls,and organochlorine pesticides in South China[J].Environ Sci Technol,2007,41(16):5647-565.
[4]Ohta S,Ishizuka D,Nlshimura H,et al.Comparison of polybrominated diphenyl ethers in fish,vegetable,and meats and levels in human milk of nursing women in Japan[J].Chemosphere,2002(46):689-696.
[5]董洪梅,魏东洋,万大娟,等.多溴联苯醚的环境行为及其降解研究进展[J].安徽农业科学,2012,40(7):9412-9415.Dong Hongmei,Wei Dongyang,Wan Dajuan,et al.Research progress in environmental behavior and degradation of polybrominated diphenyl ethers[J].Anhui Agriculture Science,2012,40(7):9412-9415.(in Chinese)
[6]Schecter A,Papke O,Harris T R,et al.Polybrominated diphenyl ether(PBDE)levels in an expanded market basket survey of us:food end estimated PBDE dietary intake by age and sex[J].Environmental Health Perspectives,2006,114(10):1515-1520.
[7]巫杨,陈东辉,Rai Kookana,等.环境中多溴联苯醚的降解研究进展[J].中国给水排水,2010,26(6):20-23.Wu Yang,Chen Donghui,Rai Kookana,et al.Study on degradation of polybrominated diphenyl ethers in environment[J].China Water&Wastewater,2010,26(6):20-23.(in Chinese)
[8]Wu N,Herrmann T,Paepke O,et al.Human exposure to PBDEs:associations of PBDE body burdens with food consumption and house dust concentrations[J].Environ Sci Technol,2007,41(5):1584-1589.
[9]Gouin T,Hamer T.Modeling the environmental fate of the polybmminated diphenyle ether s[J].Environmental Internationa1,2003,29(6):717-724.
[10]Frederiksen M,Vorkamp K,Thomsen M,et al.Human internal and external exposure to PBDEs-a review of levels and sources[J].Int J Hyg Environ Health,2009,212(2):109-134.
[11]任金亮,王平.多溴联苯醚环境行为的特征与研究进展[J].化学进展,2006,25(10):152-158.Ren Jinliang,Wang Ping.Progress and characteristics of environmental behavior of polybrominated diphenyl ethers[J].Chemical Industry and Engineering Progress,2006,25(10):152-158.(in Chinese)
[12]Palit M,Pardasani D,Gupta K,et al.Application of single drop microextraction for analysis of chemical warfare agents and related compounds in water by gas chromatography/mass spectrometry[J].Analytical Chemistry,2005(77):711-717.
[13]薛铮然,李海静.高效溴系阻燃剂十溴联苯醚生产工艺研究[J].山东化工,2002,31(4):31-32.Xue Zhengran,Li Haijing.The productive mechanism and technology research of new highly efficiency flame retardant of decabromodiphehyl ether[J].Shandong Chemical Industry,2002,31(4):31-32.(in Chinese)
[14]Shen H P,Huang C J,Lu F,et al.Comparative toxicity of PCBs and PBDEs using human cancer cell lines and zebrafish embryos[J].Asian Journal of Ecotoxicology,2009,4(5):625-633.
[15]李卓娜,孟范平,赵顺顺,等.2,2’,4,4’-四溴联苯醚(BDE-47)对4种海洋微藻的急性毒性[J].生态毒理学报,2009,4(3):435-439.Li Zhuona,Meng Fanping,Zhao Shunshun,et al.Acute toxic effects of 2,2’,4,4’-tetrabromodiphenyl ether(BDE-47)on four marine microalgae[J].Ecotoxicology,2009,4(3):435-439.(in Chinese)
[16]冯承莲,许宜平,何悦,等.十溴联苯醚(BDE209)在虹鳟体内的羟基代谢产物及其对甲状腺激素水平影响的初步研究[J].生态毒理学,2010,5(3):327-333.Feng Chenglian,Xu Yiping,He Yue,et al.Preliminary research on hydroxylated metabolites of decabromodiphenyl ether and their effects on thyroid hormone in rainbow trout(Oncorhynchus mykiss)[J].Ecotoxicology,2010,5(3):327-333.(in Chinese)
[17]孙云娜,魏东洋,李杰,等.多溴联苯醚(PBDEs)的降解技术研究进展[J].广东化工,2011,38(5):5-6.Sun Yunna,Wei Dongyang,Li Jie,et al.The research of polybrominated diphenyl ethers(PBDEs)degradation[J].Guangdong Chemical Industry,2011,38(5):5-6.(in Chinese)
[18]张向荣,薛科社.高效液相色谱法测定沉积物中的多溴联苯醚[J].光谱实验室,2009,26(4):986-989.Zhang Xiangrong,Xue Keshe.Determination of polybrominated diphenyl ethers(PBDEs)in sediment by HPLC[J].Spectroscopy Laboratory,2009,26(4):986-989.(in Chinese)
[19]王霞,李旭东.生物样品中多溴联苯醚检测的现状[J].理化检验-化学分册,2009(45):1011-1014.Wang Xia,Li Xudong.Present status of inspection of polybromodiphenyl ethers[J].PTCA(Part B:Chem Anal),2009(45):1011-1014.(in Chinese)
[20]黄英,杭义萍.加速溶剂萃取-高效液相色谱/紫外检测电子电气塑料中十溴联苯醚[J].分析试验室,2009,28(1):96-100.Huang Ying,Hang Yiping.Determination of decabrominated diphenyl ether in electronic and electrical equipments with ASE-HPLC/UV[J].Analysis Laboratory,2009,28(1):96-100.(in Chinese)
[21]俞晟,肖琳,朱东强,等.超声提取-高效液相色谱检测土壤中溴联苯醚[J].环境化学,2009,28(1):121-125.Yu Sheng,Xiao Lin,Zhu Dongqiang,et al.Determination of decabrominated diphenyl ether in soil by ultrasonic extraction and high performance lquid chromatogrphy[J].Environmental Chemistry,2009,28(1):121-125.(in Chinese)
[22]Deng D Y,Guo J,Sun G P,et al.Aerobic debromination of deca-BDE:isolation and characterization of indigenous isolate from a PBDE contaminated sediment[J].International Biodeterior Biodegradation,2011,65(3):465-469.
[23]Maltseva O V,Tsoi T V,QuensenⅢJ F,et al.Degradation of anaerobic reductive dechlorination products of Aroclor1242 by four aerobic bacteria[J].Biodegradation,1999,10(5):363-371.
[24]Abrahama W R,Nogalesb B,Golyshin P N,et al.Polychlorinated biphenyl-degrading microbial communities in soils and sediments[J].Microbiology,2002,5(3):246-253.
[25]Furukawa K,Fujihara H.Microbial degradation of polychlorinated biphenyls:biochemical and molecular features[J].Bioscience Bioengineering,2008,105(5):433-449.
[26]Vasilyeva G K,Strijakova E R.Bioremediation of soils and sediments contaminated by polychlorinated biphenyls[J].Microbiology,2007,76(6):639-653.
[2]刘汉霞,张庆华,江桂斌.多溴联苯醚及其环境问题[J].化学进展,2005,17(3):554-561.Liu Hanxia,Zhang Qinghua,Jiang Guibin.Polybrominated diphenyl ethers and its related environmental problems[J].Progress Chemistry,2005,17(3):554-561.(in Chinese)
[3]Bi X,Thomas G O,Jones K C,et a1.Exposure of electronics dismantling workers to polybrominated diphenyl ethers,polychlorinated biphenyls,and organochlorine pesticides in South China[J].Environ Sci Technol,2007,41(16):5647-565.
[4]Ohta S,Ishizuka D,Nlshimura H,et al.Comparison of polybrominated diphenyl ethers in fish,vegetable,and meats and levels in human milk of nursing women in Japan[J].Chemosphere,2002(46):689-696.
[5]董洪梅,魏东洋,万大娟,等.多溴联苯醚的环境行为及其降解研究进展[J].安徽农业科学,2012,40(7):9412-9415.Dong Hongmei,Wei Dongyang,Wan Dajuan,et al.Research progress in environmental behavior and degradation of polybrominated diphenyl ethers[J].Anhui Agriculture Science,2012,40(7):9412-9415.(in Chinese)
[6]Schecter A,Papke O,Harris T R,et al.Polybrominated diphenyl ether(PBDE)levels in an expanded market basket survey of us:food end estimated PBDE dietary intake by age and sex[J].Environmental Health Perspectives,2006,114(10):1515-1520.
[7]巫杨,陈东辉,Rai Kookana,等.环境中多溴联苯醚的降解研究进展[J].中国给水排水,2010,26(6):20-23.Wu Yang,Chen Donghui,Rai Kookana,et al.Study on degradation of polybrominated diphenyl ethers in environment[J].China Water&Wastewater,2010,26(6):20-23.(in Chinese)
[8]Wu N,Herrmann T,Paepke O,et al.Human exposure to PBDEs:associations of PBDE body burdens with food consumption and house dust concentrations[J].Environ Sci Technol,2007,41(5):1584-1589.
[9]Gouin T,Hamer T.Modeling the environmental fate of the polybmminated diphenyle ether s[J].Environmental Internationa1,2003,29(6):717-724.
[10]Frederiksen M,Vorkamp K,Thomsen M,et al.Human internal and external exposure to PBDEs-a review of levels and sources[J].Int J Hyg Environ Health,2009,212(2):109-134.
[11]任金亮,王平.多溴联苯醚环境行为的特征与研究进展[J].化学进展,2006,25(10):152-158.Ren Jinliang,Wang Ping.Progress and characteristics of environmental behavior of polybrominated diphenyl ethers[J].Chemical Industry and Engineering Progress,2006,25(10):152-158.(in Chinese)
[12]Palit M,Pardasani D,Gupta K,et al.Application of single drop microextraction for analysis of chemical warfare agents and related compounds in water by gas chromatography/mass spectrometry[J].Analytical Chemistry,2005(77):711-717.
[13]薛铮然,李海静.高效溴系阻燃剂十溴联苯醚生产工艺研究[J].山东化工,2002,31(4):31-32.Xue Zhengran,Li Haijing.The productive mechanism and technology research of new highly efficiency flame retardant of decabromodiphehyl ether[J].Shandong Chemical Industry,2002,31(4):31-32.(in Chinese)
[14]Shen H P,Huang C J,Lu F,et al.Comparative toxicity of PCBs and PBDEs using human cancer cell lines and zebrafish embryos[J].Asian Journal of Ecotoxicology,2009,4(5):625-633.
[15]李卓娜,孟范平,赵顺顺,等.2,2’,4,4’-四溴联苯醚(BDE-47)对4种海洋微藻的急性毒性[J].生态毒理学报,2009,4(3):435-439.Li Zhuona,Meng Fanping,Zhao Shunshun,et al.Acute toxic effects of 2,2’,4,4’-tetrabromodiphenyl ether(BDE-47)on four marine microalgae[J].Ecotoxicology,2009,4(3):435-439.(in Chinese)
[16]冯承莲,许宜平,何悦,等.十溴联苯醚(BDE209)在虹鳟体内的羟基代谢产物及其对甲状腺激素水平影响的初步研究[J].生态毒理学,2010,5(3):327-333.Feng Chenglian,Xu Yiping,He Yue,et al.Preliminary research on hydroxylated metabolites of decabromodiphenyl ether and their effects on thyroid hormone in rainbow trout(Oncorhynchus mykiss)[J].Ecotoxicology,2010,5(3):327-333.(in Chinese)
[17]孙云娜,魏东洋,李杰,等.多溴联苯醚(PBDEs)的降解技术研究进展[J].广东化工,2011,38(5):5-6.Sun Yunna,Wei Dongyang,Li Jie,et al.The research of polybrominated diphenyl ethers(PBDEs)degradation[J].Guangdong Chemical Industry,2011,38(5):5-6.(in Chinese)
[18]张向荣,薛科社.高效液相色谱法测定沉积物中的多溴联苯醚[J].光谱实验室,2009,26(4):986-989.Zhang Xiangrong,Xue Keshe.Determination of polybrominated diphenyl ethers(PBDEs)in sediment by HPLC[J].Spectroscopy Laboratory,2009,26(4):986-989.(in Chinese)
[19]王霞,李旭东.生物样品中多溴联苯醚检测的现状[J].理化检验-化学分册,2009(45):1011-1014.Wang Xia,Li Xudong.Present status of inspection of polybromodiphenyl ethers[J].PTCA(Part B:Chem Anal),2009(45):1011-1014.(in Chinese)
[20]黄英,杭义萍.加速溶剂萃取-高效液相色谱/紫外检测电子电气塑料中十溴联苯醚[J].分析试验室,2009,28(1):96-100.Huang Ying,Hang Yiping.Determination of decabrominated diphenyl ether in electronic and electrical equipments with ASE-HPLC/UV[J].Analysis Laboratory,2009,28(1):96-100.(in Chinese)
[21]俞晟,肖琳,朱东强,等.超声提取-高效液相色谱检测土壤中溴联苯醚[J].环境化学,2009,28(1):121-125.Yu Sheng,Xiao Lin,Zhu Dongqiang,et al.Determination of decabrominated diphenyl ether in soil by ultrasonic extraction and high performance lquid chromatogrphy[J].Environmental Chemistry,2009,28(1):121-125.(in Chinese)
[22]Deng D Y,Guo J,Sun G P,et al.Aerobic debromination of deca-BDE:isolation and characterization of indigenous isolate from a PBDE contaminated sediment[J].International Biodeterior Biodegradation,2011,65(3):465-469.
[23]Maltseva O V,Tsoi T V,QuensenⅢJ F,et al.Degradation of anaerobic reductive dechlorination products of Aroclor1242 by four aerobic bacteria[J].Biodegradation,1999,10(5):363-371.
[24]Abrahama W R,Nogalesb B,Golyshin P N,et al.Polychlorinated biphenyl-degrading microbial communities in soils and sediments[J].Microbiology,2002,5(3):246-253.
[25]Furukawa K,Fujihara H.Microbial degradation of polychlorinated biphenyls:biochemical and molecular features[J].Bioscience Bioengineering,2008,105(5):433-449.
[26]Vasilyeva G K,Strijakova E R.Bioremediation of soils and sediments contaminated by polychlorinated biphenyls[J].Microbiology,2007,76(6):639-653.