河南烟区烟草病毒病种类与分布
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摘要
为明确近年来河南省烟草病毒病的种类及分布状况,利用9种病毒血清通过酶联免疫吸附法(ELISA)对2015—2017年间河南11个烟叶产区的566份病毒病样品进行了系统检测。结果表明,河南烟区病毒病总检出率为96.47%;烟草花叶病毒(Tobacco mosaic virus, TMV)、黄瓜花叶病毒(Cucumber mosaic virus, CMV)、烟草蚀纹病毒(Tobacco etch virus, TEV)、马铃薯Y病毒(Potato virus Y, PVY)和烟草脉带花叶病毒(Tobacco vein banding mosaic virus, TVBMV)5种病毒在河南烟区危害最为严重,且TMV已发展为河南烟区病毒的优势种。全省侵染类型中单一病毒侵染率为34.63%,TMV的单一病毒侵染率最高,为26.86%;两种及以上病毒复合侵染率为61.84%,复合侵染主要存在5种类型,即TMV+CMV、TMV+CMV+TEV、TMV+TEV+PVY、TMV+TEV+TVBMV、TMV+CMV+TEV+PVY,侵染率分别为18.73%、3.36%、3.89%、5.83%和7.24%。因此,河南省11个烟叶产区的主要病毒种类确定为TMV、CMV、TEV、PVY和TVBMV,侵染类型为TMV+CMV、TMV+CMV+TEV、TMV+TEV+PVY,TMV+TEV+TVBMV、TMV+CMV+TEV+PVY。
To identify the species and distribution of tobacco virus diseases in Henan Province, 566 samples of virus diseases were collected from 11 tobacco-planting areas in Henan Province from 2015 to 2017 and analysed by ELISA with nine antibodies. The results showed that in Henan tobacco-planting areas, the infection percentage of virus diseases was 96.47%. Tobacco mosaic virus(TMV), cucumber mosaic virus(CMV),tobacco etch virus(TEV), potato virus Y(PVY) and tobacco vein banding mosaic virus(TVBMV) occurred most seriously and TMV had become a dominant virus. The infection percentage of single virus was 34.63%,and that of TMV was the highest(26.86%). The infection percentage of viruses(≥2)was 61.84%. There were five main infection types in 11 tobacco-planting areas: TMV+CMV, TMV+CMV+TEV, TMV+TEV+PVY, TMV+TEV+TVBMV and TMV+CMV+TEV+PVY, and their infection percentages were 18.73%, 3.36%, 3.89%, 5.83%and 7.24%, respectively. Therefore, the domain viruses are determined as TMV, CMV, TEV, PVY and TVBMV and the infection types are TMV+CMV, TMV+CMV+TEV, TMV+TEV+PVY, TMV+TEV+TVBMV and TMV+CMV+TEV+PVY in 11 tobacco-planting areas in Henan Province.
To identify the species and distribution of tobacco virus diseases in Henan Province, 566 samples of virus diseases were collected from 11 tobacco-planting areas in Henan Province from 2015 to 2017 and analysed by ELISA with nine antibodies. The results showed that in Henan tobacco-planting areas, the infection percentage of virus diseases was 96.47%. Tobacco mosaic virus(TMV), cucumber mosaic virus(CMV),tobacco etch virus(TEV), potato virus Y(PVY) and tobacco vein banding mosaic virus(TVBMV) occurred most seriously and TMV had become a dominant virus. The infection percentage of single virus was 34.63%,and that of TMV was the highest(26.86%). The infection percentage of viruses(≥2)was 61.84%. There were five main infection types in 11 tobacco-planting areas: TMV+CMV, TMV+CMV+TEV, TMV+TEV+PVY, TMV+TEV+TVBMV and TMV+CMV+TEV+PVY, and their infection percentages were 18.73%, 3.36%, 3.89%, 5.83%and 7.24%, respectively. Therefore, the domain viruses are determined as TMV, CMV, TEV, PVY and TVBMV and the infection types are TMV+CMV, TMV+CMV+TEV, TMV+TEV+PVY, TMV+TEV+TVBMV and TMV+CMV+TEV+PVY in 11 tobacco-planting areas in Henan Province.
引文
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[21]吉贵锋,王永,赵红朝,等.豫西烟区烟草主要病虫害发生规律及综合防治技术研究[J].农业与技术,2015,35(2):34-35.JI Guifeng,WANG Yong,ZHAO Hongchao,et al.Study on occurrence and comprehensive control techniques of main diseases and pests on tobacco in west Henan tobacco-planting areas[J].Agriculture and Technology,2015,35(2):34-35.
[22]翟玉俊,刘红海,李星亮,等.烟草病毒检测技术研究进展[J].农产品加工,2009(4):83-88.ZHAI Yujun,LIU Honghai,LI Xingliang,et al.Methods for detection of the tobacco virus[J].Academic Periodical of Farm Products Processing,2009(4):83-88.
[23]任锡毅,刘永翔,黄永会,等.基于TC-RT-PCR方法的几种烟草主要病毒病的多重检测体系的构建[J].贵州农业科学,2015,43(9):15-19,22.REN Xiyi,LIU Yongxiang,HUANG Yonghui,et al.Multiple detection system establishment of several main virus diseases based on TC-RT-PCR[J].Guizhou Agricultural Sciences,2015,43(9):15-19,22.
[24]任锡毅,刘永翔,黄永会,等.试管捕捉实时荧光RT-PCR法同时检测烟草花叶病毒病和马铃薯Y病毒病[J].江苏农业科学,2017,45(6):96-100.REN Xiyi,LIU Yongxiang,HUANG Yonghui,et al.Simultaneous detection of tobacco mosaic virus and potato Y virus by real-time fluorescent RT-PCR[J].Jiangsu Agricultural Sciences,2017,45(6):96-100.
[25]王芳,周本国,许大凤,等.利用siRNA高通量测序技术检测烟草病毒[J].植物病理学报,2015,45(1):97-100.WANG Fang,ZHOU Benguo,XU Dafeng,et al.Detection for RNA viruses in tobacco by high-throughput sequencing of siRNA[J].Acta Phytopathologica Sinica,2015,45(1):97-100.
[2]邱睿,李淑君,王海涛,等.不同烟草品种上烟蚜的繁殖特征分析[J].烟草科技,2018,51(1):15-20.QIU Rui,LI Shujun,WANG Haitao,et al.Reproductive characteristics of Myzus persicae(Sulzer)in different tobacco varieties[J].Tobacco Science&Technology,2018,51(1):15-20.
[3]WANG F,WU Q F,ZHOU B G,et al.First report of turnip yellows virus in tobacco in China[J].Plant Disease,2015,99(12):1870.
[4]孙航军,杨金广,李锡宏,等.野生番茄花叶病毒在四川侵染烟草的简报[J].植物病理学报,2015,45(6):680-684.SUN Hangjun,YANG Jinguang,LI Xihong,et al.Brief report of a Wild tomato mosaic virus isolate infecting Nicotiana tabacum in Sichuan[J].Acta Phytopathologica Sinica,2015,45(6):680-684.
[5]马国胜,何博如.烟草病毒研究现状与展望[J].中国生态农业学报,2006,14(2):150-153.MA Guosheng,HE Boru.Current status and recent advances on the tobacco viruses[J].Chinese Journal of Eco-Agriculture,2006,14(2):150-153.
[6]陈瑞泰,朱贤朝,王智发,等.全国16个主产烟省(区)烟草侵染性病害调研报告[J].中国烟草科学,1997(4):1-7.CHEN Ruitai,ZHU Xianchao,WANG Zhifa,et al.Areport of investigating and studying tobacco infectious diseases of 16 main tobacco producing provinces(regions)in China[J].Chinese Tobacco Science,1997(4):1-7.
[7]陈顺辉,顾钢,巫升鑫,等.福建省烟草病毒病种类的检测与防治[J].烟草科技,2003(9):42-46.CHEN Shunhui,GU Gang,WU Shengxin,et al.Strains detection and control of tobacco virus diseases in Fujian Province[J].Tobacco Science&Technology,2003(9):42-46.
[8]青玲,牛颜冰,吴楚钊,等.部分烟区烟草病毒病病原检测及复合侵染分析[J].烟草科技,2009(12):58-64.QING Ling,NIU Yanbing,WU Chuzhao,et al.Virus detection of tobacco viral disease and analysis of viruses mixed-infection in some tobacco growing areas[J].Tobacco Science&Technology,2009(12):58-64.
[9]江彤,李爽,陈伟,等.安徽省烟草病毒种类的血清学鉴定[J].中国烟草科学,2009,30(5):50-53.JIANG Tong,LI Shuang,CHEN Wei,et al.Serological identification of tobacco viruses derived from Anhui Province[J].Chinese Tobacco Science,2009,30(5):50-53.
[10]李斌,夏先全,姚革,等.四川烟草主产区烟草病毒种类及流行分析[J].西南农业学报,2007,20(6):1237-1240.LI Bin,XIA Xianquan,YAO Ge,et al.The species and epidemiology of virus in main tobacco regions in Sichuan[J].Southwest China Journal of Agricultural Sciences,2007,20(6):1237-1240.
[11]李义强,刘金亮,王凤龙,等.山东、河南烟草病毒病发生种类、发病规律研究[J].中国烟草学报,2004,10(3):7-13.LI Yiqiang,LIU Jinliang,WANG Fenglong,et al.Occurrence and control of tobacco viral diseases in Shandong and Henan Province[J].Acta Tabacaria Sinica,2004,10(3):7-13.
[12]罗朝鹏,杨军,王燃,等.我国烟区病毒病种类的血清学鉴定[J].烟草科技,2012(12):72-77.LUO Zhaopeng,YANG Jun,WANG Ran,et al.Serological identification of viruses infecting tobacco in major tobacco growing areas of China[J].Tobacco Science&Technology,2012(12):72-77.
[13]巢进,张洪波,肖启明,等.湖南省烟草病毒病种类的鉴定[J].湖南农业科学,2008(4):104-106,108.CHAO Jin,ZHANG Hongbo,XIAO Qiming,et al.Identification of tobacco virus types in Hunan[J].Hunan Agricultural Sciences,2008(4):104-106,108.
[14]李金岭,罗晶,牛小义,等.陕西省烟草主要病毒病种类及植物带毒的检测[J].西北农林科技大学学报(自然科学版),2013,41(1):78-84.LI Jinling,LUO Jing,NIU Xiaoyi,et al.Detection and analysis of main tobacco viruses and viruliferous plants in Shaanxi Province[J].Journal of Northwest A&FUniversity(Natural Science),2013,41(1):78-84.
[15]Roggero P,Accotto G P,Ciuffo M,et al.First report of Tobacco vein banding mosaic virus in China(Xi’an,Shaanxi Province)in Datura stramonium and tobacco[J].Plant Disease,2000,84(10):1152.
[16]李淑君,王海涛,陈玉国,等.2000年烟草病毒病大发生概况与原因分析[J].烟草科技,2001(1):44-46.LI Shujun,WANG Haitao,CHEN Yuguo,et al.Analysis on epidemical reason of tobacco virus diseases in 2000[J].Tobacco Science&Technology,2001(1):44-46.
[17]李义强,王维超,刘建坤,等.豫中烟区烟草病毒病种类鉴定及对策分析[J].中国烟草科学,2003,24(1):40-42.LI Yiqiang,WANG Weichao,LIU Jiankun,et al.Identification of tobacco virus diseases in the middle of Henan Province[J].Chinese Tobacco Science,2003,24(1):40-42.
[18]张全民,张晓娟.平顶山烟区有翅蚜迁飞动态及病毒病流行规律研究[J].河南农业,2014(1):40-42.ZHANG Quanmin,ZHANG Xiaojuan.Study on dynamics of Myzus persicae(Sulzer)and epidemic rule of viruses in Pingdingshan tobacco-planting areas[J].Agriculture of Henan,2014(1):40-42.
[19]邓阳春,黄建国.长期连作对烤烟产量和土壤养分的影响[J].植物营养与肥料学报,2010,16(4):840-845.DENG Yangchun,HUANG Jianguo.Effect of long continuous cropping on the yields of flue-cured tobacco and nutrients in soils[J].Plant Nutrition and Fertilizer Science,2010,16(4):840-845.
[20]彭曙光.我国烟草病毒病的发生及综合防治研究进展[J].江西农业学报,2011,23(1):115-117.PENG Shuguang.Research advance in occurrence and integrated control of tobacco virus diseases in China[J].Acta Agriculturae Jiangxi,2011,23(1):115-117.
[21]吉贵锋,王永,赵红朝,等.豫西烟区烟草主要病虫害发生规律及综合防治技术研究[J].农业与技术,2015,35(2):34-35.JI Guifeng,WANG Yong,ZHAO Hongchao,et al.Study on occurrence and comprehensive control techniques of main diseases and pests on tobacco in west Henan tobacco-planting areas[J].Agriculture and Technology,2015,35(2):34-35.
[22]翟玉俊,刘红海,李星亮,等.烟草病毒检测技术研究进展[J].农产品加工,2009(4):83-88.ZHAI Yujun,LIU Honghai,LI Xingliang,et al.Methods for detection of the tobacco virus[J].Academic Periodical of Farm Products Processing,2009(4):83-88.
[23]任锡毅,刘永翔,黄永会,等.基于TC-RT-PCR方法的几种烟草主要病毒病的多重检测体系的构建[J].贵州农业科学,2015,43(9):15-19,22.REN Xiyi,LIU Yongxiang,HUANG Yonghui,et al.Multiple detection system establishment of several main virus diseases based on TC-RT-PCR[J].Guizhou Agricultural Sciences,2015,43(9):15-19,22.
[24]任锡毅,刘永翔,黄永会,等.试管捕捉实时荧光RT-PCR法同时检测烟草花叶病毒病和马铃薯Y病毒病[J].江苏农业科学,2017,45(6):96-100.REN Xiyi,LIU Yongxiang,HUANG Yonghui,et al.Simultaneous detection of tobacco mosaic virus and potato Y virus by real-time fluorescent RT-PCR[J].Jiangsu Agricultural Sciences,2017,45(6):96-100.
[25]王芳,周本国,许大凤,等.利用siRNA高通量测序技术检测烟草病毒[J].植物病理学报,2015,45(1):97-100.WANG Fang,ZHOU Benguo,XU Dafeng,et al.Detection for RNA viruses in tobacco by high-throughput sequencing of siRNA[J].Acta Phytopathologica Sinica,2015,45(1):97-100.