免疫增强剂紫锥菊(Echinacea purpurea)提取物对大菱鲆(Scophthalmus maximus)头肾内非特异性免疫基因表达量的影响
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摘要
通过给大菱鲆(Scophthalmus maximus)注射不同浓度的紫锥菊(Echinacea purpurea)提取物,测定其非特异性免疫指标的变化水平,研究其对大菱鲆的头肾内非特异性免疫分子基因相对表达量的影响,进而为其在大菱鲆养殖生产过程中推广应用提供科学依据。试验选取体重(250±20)g的大菱鲆作为试验动物,分别注射10、20、40mg/m L浓度的紫锥菊提取物,试验共进行28d,注射试验结束后,分别从高、中、低剂量组及空白对照组选出6尾大菱鲆,分别取出大菱鲆的头肾并提取总RNA。采用?Ct法进行目标基因的荧光定量分析,再应用SPSS17.0软件对获得的实验数据进行单因素方差分析。研究结果表明,紫锥菊提取物能够不同程度地提高大菱鲆头肾内非特异性免疫分子基因相对表达量。注射紫锥菊提取物28d后对大菱鲆头肾中Lysozyme基因相对表达量的影响显著(P<0.05),对C3补体基因相对表达量的影响极显著(P<0.01),对Transferrin基因相对表达量的影响显著(P<0.05),对TGF-β1基因相对表达量的影响极显著(P<0.01),对IL-1h基因相对表达量的影响极显著(P<0.01),本试验研究表明,紫锥菊提取物能够显著提高大菱鲆头肾内非特异性免疫分子基因的相对表达量。
To determine the effect of purple coneflower Echinacea purpurea extract on the non-specific immune response of turbot Scophthalmus maximus, four groups of six turbot each in weight of(250±20)g were injected with the extract at concentrations of 0(the control), 10(low), 20(intermediate), and 40 mg/m L(high) respectively. After 28 d of experiment, the relative expression of specific immune gene in the head kidney and the total RNA were studied quantitatively. Results show that the relative expression of kidney nonspecific immune gene was boosted after the injection, which will provide a scientific basis for the application of the extract in turbot farming
To determine the effect of purple coneflower Echinacea purpurea extract on the non-specific immune response of turbot Scophthalmus maximus, four groups of six turbot each in weight of(250±20)g were injected with the extract at concentrations of 0(the control), 10(low), 20(intermediate), and 40 mg/m L(high) respectively. After 28 d of experiment, the relative expression of specific immune gene in the head kidney and the total RNA were studied quantitatively. Results show that the relative expression of kidney nonspecific immune gene was boosted after the injection, which will provide a scientific basis for the application of the extract in turbot farming
引文
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任永林,2008.紫锥菊提取物对鲤生产性能和免疫功能的影响.雅安:四川农业大学硕士学位论文
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汤菊芬,吴灶和,简纪常等,2011.注射黄芪多糖对吉富罗非鱼c型溶菌酶基因表达量的影响.广东海洋大学学报,31(1):58-61
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胡兵,刘军,侯永清等,2008.黄芪多糖对异育银鲫非特异性免疫力的影响.水利渔业,28(3):108-111
唐雪莲,付京花,李志华等,2012.紫锥菊提取物对彭泽鲫生长和抗氧化反应的影响.饲料工业,33(14):20-22
黄智慧,马爱军,2010.大菱鲆体表黏液细胞组化研究及高温胁迫对其类型和分布的影响.水产学报,34(5):719-725
韩雯,王秀华,王玉娟等,2014.皂角苷对大菱鲆非特异免疫的增强作用.水产学报,38(7):999-1008
雷霁霖,2000.海水养殖新品种介绍--大菱鲆.中国水产,(4):38-39
雷霁霖,2005.海水鱼类养殖理论与技术.北京:中国农业出版社,321-322
雷霁霖,门强,2002.大菱鲆人工繁殖与养殖技术讲座(Ⅱ).齐鲁渔业,19(10):47-48
蔡中华,陈成勋,刑克智等,1998.四种中药对鲤鱼非特异性免疫功能的影响.天津农学院学报,5(2):31-34
ArdóL,Yin G J,Xu P,2008.Chinese herbs(Astragalus membranaceus and Lonicera japonica)and boron enhance the non-specific immune response of Nile tilapia(Oreochromis niloticus)and resistance against Aeromonas hydrophila.Aquaculture,275(1-4):26-33
Bachali S,Bailly X,Jollès J et al,2004.The lysozyme of the starfish Asterias rubens.A paradigmatic type i lysozyme.European Journal of Biochemistry,271(2):237-242
Brattgjerd S,Evensen?,1996.A sequential light microscopic and ultrastructural study on the uptake and handling of Vibrio salmonicida of the head kidney in experimentally infected Atlantic salmon(Salmo salar L.).Veterinary Pathology,33(5):55-65
Cádiz,2015.Asociación Empresarial de Productores de Cultivos Marinos(APROMAR).La Acuicultura Marina de Peces en Espa?a
Food and Agriculture Organization of the United Nations(FAO),2011.The State of World Fisheries and Aquaculture(SOFIA)2010.Rome:FAO
Liu M,Zhang S C,Liu Z H et al,2006.Characterization,organization and expression of Amphi Lys C,an acidic c-type lysozyme gene in amphioxus Branchiostoma belcheri tsingtauense.Gene,367:110-117
Liu Y L,Sun J H,Zhang J et al,2003.Effects of transferrin on the growth and proliferation of porcine hepatocytes:a comparison with epidermal growth factor and nicotinamide.Chinese Medical Journal,116(8):1223-1227
Oppenheimer S J,2001.Iron and its relation to immunity and infectious disease.Journal of Nutrition,131(2):616S-635S
Press C M,Evensen?,1999.The morphology of the immune system in teleost fishes.Fish&Shellfish Immunology,9(4):309-318
Sotelo-Mundo R R,Islas-Osuna M A,de-la-Re-Vega E et al,2003.c DNA cloning of the lysozyme of the white shrimp Penaeus vannamei.Fish&Shellfish Immunology,15(4):325-331
Steel D M,Whitehead A S,1994.The major acute phase reactants:C-reactive protein,serum amyloid P component and serum amyloid A protein.Immunology Today,15(2):81-88
王高学,白冰,崔婧等,2006.灰树花多糖对鲫鱼免疫功能的影响.水产科学,25(1):9-12
任永林,2008.紫锥菊提取物对鲤生产性能和免疫功能的影响.雅安:四川农业大学硕士学位论文
庄政,2008.嗜水气单胞菌亚单位成分卵黄抗体在鳗鲡养殖中应用.福州:福建农林大学硕士学位论文,10-11
汤菊芬,吴灶和,简纪常等,2011.注射黄芪多糖对吉富罗非鱼c型溶菌酶基因表达量的影响.广东海洋大学学报,31(1):58-61
孙永欣,徐永平,汪婷婷等,2009.黄芪多糖对仿刺参体腔细胞中溶菌酶基因表达量的影响.水产科学,28(10):572-574
胡兵,刘军,侯永清等,2008.黄芪多糖对异育银鲫非特异性免疫力的影响.水利渔业,28(3):108-111
唐雪莲,付京花,李志华等,2012.紫锥菊提取物对彭泽鲫生长和抗氧化反应的影响.饲料工业,33(14):20-22
黄智慧,马爱军,2010.大菱鲆体表黏液细胞组化研究及高温胁迫对其类型和分布的影响.水产学报,34(5):719-725
韩雯,王秀华,王玉娟等,2014.皂角苷对大菱鲆非特异免疫的增强作用.水产学报,38(7):999-1008
雷霁霖,2000.海水养殖新品种介绍--大菱鲆.中国水产,(4):38-39
雷霁霖,2005.海水鱼类养殖理论与技术.北京:中国农业出版社,321-322
雷霁霖,门强,2002.大菱鲆人工繁殖与养殖技术讲座(Ⅱ).齐鲁渔业,19(10):47-48
蔡中华,陈成勋,刑克智等,1998.四种中药对鲤鱼非特异性免疫功能的影响.天津农学院学报,5(2):31-34
ArdóL,Yin G J,Xu P,2008.Chinese herbs(Astragalus membranaceus and Lonicera japonica)and boron enhance the non-specific immune response of Nile tilapia(Oreochromis niloticus)and resistance against Aeromonas hydrophila.Aquaculture,275(1-4):26-33
Bachali S,Bailly X,Jollès J et al,2004.The lysozyme of the starfish Asterias rubens.A paradigmatic type i lysozyme.European Journal of Biochemistry,271(2):237-242
Brattgjerd S,Evensen?,1996.A sequential light microscopic and ultrastructural study on the uptake and handling of Vibrio salmonicida of the head kidney in experimentally infected Atlantic salmon(Salmo salar L.).Veterinary Pathology,33(5):55-65
Cádiz,2015.Asociación Empresarial de Productores de Cultivos Marinos(APROMAR).La Acuicultura Marina de Peces en Espa?a
Food and Agriculture Organization of the United Nations(FAO),2011.The State of World Fisheries and Aquaculture(SOFIA)2010.Rome:FAO
Liu M,Zhang S C,Liu Z H et al,2006.Characterization,organization and expression of Amphi Lys C,an acidic c-type lysozyme gene in amphioxus Branchiostoma belcheri tsingtauense.Gene,367:110-117
Liu Y L,Sun J H,Zhang J et al,2003.Effects of transferrin on the growth and proliferation of porcine hepatocytes:a comparison with epidermal growth factor and nicotinamide.Chinese Medical Journal,116(8):1223-1227
Oppenheimer S J,2001.Iron and its relation to immunity and infectious disease.Journal of Nutrition,131(2):616S-635S
Press C M,Evensen?,1999.The morphology of the immune system in teleost fishes.Fish&Shellfish Immunology,9(4):309-318
Sotelo-Mundo R R,Islas-Osuna M A,de-la-Re-Vega E et al,2003.c DNA cloning of the lysozyme of the white shrimp Penaeus vannamei.Fish&Shellfish Immunology,15(4):325-331
Steel D M,Whitehead A S,1994.The major acute phase reactants:C-reactive protein,serum amyloid P component and serum amyloid A protein.Immunology Today,15(2):81-88