去掉角质层边缘前后企鹅珍珠贝形态性状的相关性及通径分析
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摘要
根据观察,企鹅珍珠贝的形态差异较大,而去掉黑色角质层边缘的贝壳部分变化可能较小。为了更好地研究企鹅珍珠贝的生长及珍珠质颜色的形成,实验随机选取企鹅珍珠贝300枚,测量其总重、壳高、足丝直径等9个数量性状。杀贝后取左壳,用工具削去角质层边缘后测量壳重、壳高等11个相关指标。除重量性状、足丝直径和壳宽外,其余形态性状通过拍照结合Photoshop软件建立坐标系和像素测距测得。所得数据用SPSS 19进行分析。结果表明:去掉黑色边缘的贝壳壳高、对角线与壳长之间的相关性优于完整贝壳,说明去掉角质层边缘的贝壳形态变化更规律,具有更好的研究意义和价值。除足丝直径外,绝大部分性状间呈显著相关性(p<0.05)。依据逐步回归建立了线性回归方程:Y1=-88.969+2.629X_(18)+8.321X_1+15.3X_2。通径分析中右壳重对总重的影响最大,其次是壳高和壳宽。实验结果为企鹅珍珠贝的选择育种等方面的应用提供了重要参考依据。
Based on the observation, Pteria pengu in varied greatly in morphology, while the parts of shells without black cuticle edge probably had less changes. In order to make better research about the growth and nacre color formation of P. penguin, we measured 9 quantitative traits of 300 randomly chosen P. penguins, such as the total weight, the height of the shell and the foot-filament diameter. After killing the P. penguin, the left shell was taken and 11 related indexes such as shell weight and shell height was measured after cutting off the cuticle edge.All the morphological traits were measured by taking photos and using Photoshop software to establish coordinate system and pixel distance measurement except weight traits, foot-filament diameter and shell width. The data obtained were analyzed by SPSS 19. The result showed that: the correlation among the shell height, diagonal line and shell length of the shell without black edge was better than that of the complete shell, which indicated that morphological traits of the shell without cuticle edge changed more regularly and had better research significance and value. There were significant correlation(p<0.05) among the most traits except the foot-filament diameter.The linear regression equation was built according to the stepwise regression: Y1=-88.969+2.629X_(18)+8.321X_1+15.3X_2.In path analysis, the weight of right shell had the greatest influence on total weight, followed by shell height and shell width. The results could provide important references for the selective breeding of P. penguin and so on.
Based on the observation, Pteria pengu in varied greatly in morphology, while the parts of shells without black cuticle edge probably had less changes. In order to make better research about the growth and nacre color formation of P. penguin, we measured 9 quantitative traits of 300 randomly chosen P. penguins, such as the total weight, the height of the shell and the foot-filament diameter. After killing the P. penguin, the left shell was taken and 11 related indexes such as shell weight and shell height was measured after cutting off the cuticle edge.All the morphological traits were measured by taking photos and using Photoshop software to establish coordinate system and pixel distance measurement except weight traits, foot-filament diameter and shell width. The data obtained were analyzed by SPSS 19. The result showed that: the correlation among the shell height, diagonal line and shell length of the shell without black edge was better than that of the complete shell, which indicated that morphological traits of the shell without cuticle edge changed more regularly and had better research significance and value. There were significant correlation(p<0.05) among the most traits except the foot-filament diameter.The linear regression equation was built according to the stepwise regression: Y1=-88.969+2.629X_(18)+8.321X_1+15.3X_2.In path analysis, the weight of right shell had the greatest influence on total weight, followed by shell height and shell width. The results could provide important references for the selective breeding of P. penguin and so on.
引文
Chang Y.Q.,Z hang C.S.,Cao X.B.,Yang X.G.,and Li Y.F.,2008,Effect of morphometrical traits on weight in one-year old yesso scallop Patinopecten yessoensis,Dalian Shuichan Xuebao(Journal of Dalian Fisheries University),23(5):330-335(常亚青,张存善,曹学彬,杨小刚,李云峰,2008,虾夷扇贝形态性状对重量性状的影响效果分析,大连水产学院学报,23(5):330-335)
Gu Z.F.,Wang Q.Y.,Fang J.G.,Shi Y.H.,Wang Y.,and Wang A.M.,2009,Growth of cultured winged pearl oyster(Pteria penguin)in Li'an lagoon,Hainan,Haiyang Yu Huzhao(Oceanologia Et Limnologia Sinica),40(4):423-429(顾志峰,王清印,方建光,石耀华,王嫣,王爱民,2009,企鹅珍珠贝(Pteria penguin)在海南黎安港的生长特征,海洋与湖沼,40(4):423-429)
Kishore P.,Sou thgate P.,Seeto J.,and Hunter J.,2015,Factors influencing the quality of half-pearls(mabé)produced by the winged pearl oyster,Pteria penguin(R觟ding,1758),Aquaculture Research,46(4):769-776
Lai S.M.,Wang R.J.,Jiang L.X.,Qin H.,and Li Y.Q.,2015,Path analysis between morphological characters and body weight of Chinese tiger-head crab Orithyia sinica by multiple regression,Haiyang Yu Huzhao(Oceanologia Et Limnologia Sinica),46(6):1438-1443(来守敏,王仁杰,姜令绪,秦浩,李玉全,2015,逐步线性回归法实现中华虎头蟹(Orithyia sinica)形态指标与体重的通径分析,海洋与湖沼,46(6):1438-1443)
Li Z.M.,Liu Z.G.,Wang H.,and Tang X.Y.,2011,Effects of main economic traints on body weight of Pteria penguin,Haiyang Yu Huzhao(Oceanologia Et Limnologia Sinica),42(6):798-803(栗志民,刘志刚,王辉,唐西岳,2011,企鹅珍珠贝(Pteria penguin)主要经济性状对体重的影响效果分析,海洋与湖沼,42(6):798-803)
Linoy C.L.,Abraham K.J.,Shanmugam K.,Prakash K.,Kripa V.,Velayudhan T.S.,and Mohamed K.S.,2011,Biometric relationships of the black winged pearl oyster,Pteria penguin(Roding,1798)from Andaman and Nicobar Islands,Ind.J.Fish.,58(3):139-143
Liu X.L.,Chang Y.Q.,Xiang J.H.,Song J.,and Ding J.,2002,Analysis of effects of shell size characters on live weight in Chinese scallop Chlamys farreri,Haiyang Yu Huzhao(O-ceanologia et Limnologia Sinica),33(6):673-678(刘小林,常亚青,相建海,宋坚,丁君,2002,栉孔扇贝壳尺寸性状对活体重的影响效果分析,海洋与湖沼,33(6):673-678)
Liu Z.G.,Wang H.,Sun X.Z.,Du X.D.,and Li L.B.,2007,Analysis of determination effects of economic traits on body weight of Pinctada martensii(Dunker),Guangdong Haiyang Daxue Xuebao(Journal of Zhanjiang Ocean University),27(4):15-20(刘志刚,王辉,孙小真,杜晓东,李雷斌,2007,马氏珠母贝经济性状对体重决定效应分析,广东海洋大学学报,27(4):15-20)
Mao Y.,Liang F.L.,Yu X.Y.,and Ye F.L.,2003,Preliminary observations on implanation efficiency of cultured round pearls with pearl oyster,pteria(magnavicula)penguin roding,Haiyang Kexue(Marine Sciences),27(11):1-4(毛勇,梁飞龙,余祥勇,叶富良,2003,企鹅珍珠贝游离珠插核效果的初步观察,海洋科学,27(11):1-4)
Milione M.,and Southgate P.,2012,Growth of the winged pearl oyster,Pteria penguin,at dissimilar sites in northeastern Australia,J.Shellfish Res.,31(1):13-20
Peng M.,Chen X.L.,Jiang W.M.,Yang C.L.,and Li Y.M.,2012,The population genetic diversity of different geographical pteria penguin revealed by f AFLP analysis,Shuisheng Shengwu Xuebao(Acta Hydrobiologica Sinica),36(1):102-108(彭敏,陈秀荔,蒋伟明,杨春玲,李咏梅,2012,企鹅珍珠贝不同地理群体遗传多样性的f AFLP分析,水生生物学报,36(1):102-108)
Qi L.J.,Huang Y.L.,Zhou Z.Y.,and Zhou Z.Y.,2011,The growth of the screw dislocation of nacreous layer on Pteria penguin,Sci.China Earth Sci.,54(7):951-958
Toro J.E.,Aguila P.,and Vergara A.M.,1996,Spatial variation in response to selection for live weight and shell length from data on individually tagged chilean native oysters(ostrea chilensis philippi,1845),Aquaculture,146(1-2):27-36
Wang H.,Liu Z.G.,and Fu S.W.,2007,Correlation between morphological traits and body weight of scapharca subcrenata from south china sea,Redai Haiyang Xuebao(Journal of Tropical Oceanography),26(6):58-61(王辉,刘志刚,符世伟,2007,南海毛蚶形态特征对体重的相关分析,热带海洋学报,26(6):58-61)
Wang Y.L.,2010,Studies on microsatellite marker and morphological contrastive analysis of wild and cultivated Pteria penguin(Roding),Thesis for M.S.,Guangdong Ocean University,Supervisor:Wang M.F.,pp.11-13(王永丽,2010,企鹅珍珠贝野生与养殖群体的微卫星标记及形态学对比分析,硕士学位论文,广东海洋大学,导师:王梅芳,pp.11-13)
Wassnig M.,and Southgate P.C.,2007,The effects of egg stocking density and antibiotic treatment on survival and development of winged pearl oyster(Pteria Penguin,R觟ding 1798)Embryos,Journal of Shellfish Research,30:103-107
Wassnig M.,and Southgate P.C.,2011,Embryonic and larval development of Pteria penguin(R觟ding,1798)(Bivalvia:Pteriidae),Journal of Molluscan Studies,78(1):134-141
Yan Z.,Gu Z.F.,Zhang H.,Wang Y.,Shi Y.H.,Zhan X.,and Wang A.M.,2012,Correlation and path analysis of major quantitative traits of Chlamys nobilis in Sanya,Nanfang Shuichan Kexue(South China Fisheries Science),8(3):34-38(鄢朝,顾志峰,章华忠,王嫣,石耀华,战欣,王爱民,2012,华贵栉孔扇贝数量性状的相关性及通径分析,南方水产科学,8(3):34-38)
Yu X.Y.,Wang M.F.,Liang F.L.,and Liu Y.,2005,Differentiation of isozymes from different tissues of Pteria penguin roding,Zhongguo Shuichan Kexue(Journal of Fishery Sciences of China),12(2):201-206(余祥勇,王梅芳,梁飞龙,刘永,2005,企鹅珍珠贝不同组织同工酶表达的差异,中国水产科学,12(2):201-206)
Gu Z.F.,Wang Q.Y.,Fang J.G.,Shi Y.H.,Wang Y.,and Wang A.M.,2009,Growth of cultured winged pearl oyster(Pteria penguin)in Li'an lagoon,Hainan,Haiyang Yu Huzhao(Oceanologia Et Limnologia Sinica),40(4):423-429(顾志峰,王清印,方建光,石耀华,王嫣,王爱民,2009,企鹅珍珠贝(Pteria penguin)在海南黎安港的生长特征,海洋与湖沼,40(4):423-429)
Kishore P.,Sou thgate P.,Seeto J.,and Hunter J.,2015,Factors influencing the quality of half-pearls(mabé)produced by the winged pearl oyster,Pteria penguin(R觟ding,1758),Aquaculture Research,46(4):769-776
Lai S.M.,Wang R.J.,Jiang L.X.,Qin H.,and Li Y.Q.,2015,Path analysis between morphological characters and body weight of Chinese tiger-head crab Orithyia sinica by multiple regression,Haiyang Yu Huzhao(Oceanologia Et Limnologia Sinica),46(6):1438-1443(来守敏,王仁杰,姜令绪,秦浩,李玉全,2015,逐步线性回归法实现中华虎头蟹(Orithyia sinica)形态指标与体重的通径分析,海洋与湖沼,46(6):1438-1443)
Li Z.M.,Liu Z.G.,Wang H.,and Tang X.Y.,2011,Effects of main economic traints on body weight of Pteria penguin,Haiyang Yu Huzhao(Oceanologia Et Limnologia Sinica),42(6):798-803(栗志民,刘志刚,王辉,唐西岳,2011,企鹅珍珠贝(Pteria penguin)主要经济性状对体重的影响效果分析,海洋与湖沼,42(6):798-803)
Linoy C.L.,Abraham K.J.,Shanmugam K.,Prakash K.,Kripa V.,Velayudhan T.S.,and Mohamed K.S.,2011,Biometric relationships of the black winged pearl oyster,Pteria penguin(Roding,1798)from Andaman and Nicobar Islands,Ind.J.Fish.,58(3):139-143
Liu X.L.,Chang Y.Q.,Xiang J.H.,Song J.,and Ding J.,2002,Analysis of effects of shell size characters on live weight in Chinese scallop Chlamys farreri,Haiyang Yu Huzhao(O-ceanologia et Limnologia Sinica),33(6):673-678(刘小林,常亚青,相建海,宋坚,丁君,2002,栉孔扇贝壳尺寸性状对活体重的影响效果分析,海洋与湖沼,33(6):673-678)
Liu Z.G.,Wang H.,Sun X.Z.,Du X.D.,and Li L.B.,2007,Analysis of determination effects of economic traits on body weight of Pinctada martensii(Dunker),Guangdong Haiyang Daxue Xuebao(Journal of Zhanjiang Ocean University),27(4):15-20(刘志刚,王辉,孙小真,杜晓东,李雷斌,2007,马氏珠母贝经济性状对体重决定效应分析,广东海洋大学学报,27(4):15-20)
Mao Y.,Liang F.L.,Yu X.Y.,and Ye F.L.,2003,Preliminary observations on implanation efficiency of cultured round pearls with pearl oyster,pteria(magnavicula)penguin roding,Haiyang Kexue(Marine Sciences),27(11):1-4(毛勇,梁飞龙,余祥勇,叶富良,2003,企鹅珍珠贝游离珠插核效果的初步观察,海洋科学,27(11):1-4)
Milione M.,and Southgate P.,2012,Growth of the winged pearl oyster,Pteria penguin,at dissimilar sites in northeastern Australia,J.Shellfish Res.,31(1):13-20
Peng M.,Chen X.L.,Jiang W.M.,Yang C.L.,and Li Y.M.,2012,The population genetic diversity of different geographical pteria penguin revealed by f AFLP analysis,Shuisheng Shengwu Xuebao(Acta Hydrobiologica Sinica),36(1):102-108(彭敏,陈秀荔,蒋伟明,杨春玲,李咏梅,2012,企鹅珍珠贝不同地理群体遗传多样性的f AFLP分析,水生生物学报,36(1):102-108)
Qi L.J.,Huang Y.L.,Zhou Z.Y.,and Zhou Z.Y.,2011,The growth of the screw dislocation of nacreous layer on Pteria penguin,Sci.China Earth Sci.,54(7):951-958
Toro J.E.,Aguila P.,and Vergara A.M.,1996,Spatial variation in response to selection for live weight and shell length from data on individually tagged chilean native oysters(ostrea chilensis philippi,1845),Aquaculture,146(1-2):27-36
Wang H.,Liu Z.G.,and Fu S.W.,2007,Correlation between morphological traits and body weight of scapharca subcrenata from south china sea,Redai Haiyang Xuebao(Journal of Tropical Oceanography),26(6):58-61(王辉,刘志刚,符世伟,2007,南海毛蚶形态特征对体重的相关分析,热带海洋学报,26(6):58-61)
Wang Y.L.,2010,Studies on microsatellite marker and morphological contrastive analysis of wild and cultivated Pteria penguin(Roding),Thesis for M.S.,Guangdong Ocean University,Supervisor:Wang M.F.,pp.11-13(王永丽,2010,企鹅珍珠贝野生与养殖群体的微卫星标记及形态学对比分析,硕士学位论文,广东海洋大学,导师:王梅芳,pp.11-13)
Wassnig M.,and Southgate P.C.,2007,The effects of egg stocking density and antibiotic treatment on survival and development of winged pearl oyster(Pteria Penguin,R觟ding 1798)Embryos,Journal of Shellfish Research,30:103-107
Wassnig M.,and Southgate P.C.,2011,Embryonic and larval development of Pteria penguin(R觟ding,1798)(Bivalvia:Pteriidae),Journal of Molluscan Studies,78(1):134-141
Yan Z.,Gu Z.F.,Zhang H.,Wang Y.,Shi Y.H.,Zhan X.,and Wang A.M.,2012,Correlation and path analysis of major quantitative traits of Chlamys nobilis in Sanya,Nanfang Shuichan Kexue(South China Fisheries Science),8(3):34-38(鄢朝,顾志峰,章华忠,王嫣,石耀华,战欣,王爱民,2012,华贵栉孔扇贝数量性状的相关性及通径分析,南方水产科学,8(3):34-38)
Yu X.Y.,Wang M.F.,Liang F.L.,and Liu Y.,2005,Differentiation of isozymes from different tissues of Pteria penguin roding,Zhongguo Shuichan Kexue(Journal of Fishery Sciences of China),12(2):201-206(余祥勇,王梅芳,梁飞龙,刘永,2005,企鹅珍珠贝不同组织同工酶表达的差异,中国水产科学,12(2):201-206)