摘要
目的:检测ED大鼠模型阴茎组织中降钙素基因相关肽(CGRP)、血管活性肠肽(VIP)的表达,并探讨两者在ED中的作用机制。方法:100只正常雄性SD大鼠,随机抽取10只为正常对照组,余90只为造模组,采用湿寒饮食和环境的干预条件建立ED大鼠模型,20周后通过阿朴吗啡(APO)勃起实验,筛选出44只ED大鼠模型,并随机将其分为ED模型组、自然恢复组、给药组,伊木萨克片250 mg/kg干预2~3周后,免疫组化及Western印迹方法检测阴茎组织中CGRP、VIP的表达。结果:免疫组化检测大鼠阴茎组织中CGRP表达:ED模型组(150. 0±43. 3)、自然恢复组(165. 9±40. 7)较正常对照组(227. 3±42. 5)组明显减少(P <0. 05);给药组(255. 0±38. 7)组较ED模型组(150. 0±43. 3)、自然恢复组(165. 9±40. 7)显著升高(P <0. 05)。免疫组化检测大鼠阴茎组织中VIP表达:ED模型组(36. 4±13. 1)、自然恢复组(67. 5±29. 0)较正常对照组(175. 0±45. 6)显著降低(P <0. 05);给药组(167. 5±42. 6)较ED模型组(36. 4±13. 1)、自然恢复组(67. 5±29. 0)显著升高(P <0. 05)。结论:ED大鼠模型阴茎组织中CGRP、VIP明显减少,伊木萨克片可能通过上调CGRP、VIP表达对ED大鼠发挥作用。
Objective: To determine the expressions of calcitonin gene-related peptide( CGRP) and vasoactive intestinal peptide( VIP) in the penile tissue of the ED rat model and explore their action mechanisms. Methods: An ED model was established in 44 mature male SD rats by feeding them on a spinach + coriander diet in a cold-wet environment and another 10 were taken as normal controls. Then the model rats were randomly divided into an ED model control group( n = 15) treated by gavage of distilled water in the same modeling environment,a spontaneous recovery group( n = 15) treated by gavage of distilled water in the normal environment,and a medication group( n = 14) treated intragastrically with Yimusake Tablets at 250 mg/kg qd. After 2-3 weeks of intervention,the expressions of CGRP and VIP in the penile tissue were detected by immunohistochemistry and Western blot. Results: Immunohistochemistry showed that,after 2 weeks of intervention,both the expressions of CGRP and VIP in the rat penile tissue were significantly lower in the ED model control( 150. 0 ± 43. 3 and 36. 4 ± 13. 1) and the spontaneous recovery group( 165. 9 ± 40. 7 and 67. 5 ± 29. 0) than in the normal control( 227. 3 ± 42. 5 and 175. 0 ± 45. 6)( P < 0. 05),but remarkably higher in the medication group( 255. 0 ± 38. 7 and 167. 5 ± 42. 6) than those in the ED model control and spontaneous recovery groups( P < 0. 05).Conclusion: The expressions of CGRP and VIP were significantly down-regulated in the ED rat model,and Yimusake Tablets improved ED by up-regulating their expressions.
引文
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