摘要
随着肉制品鉴别技术的进步,假羊肉的鉴别手段越来越多样化,鉴别准确率与效率也越来越高。目前,羊肉的鉴别方法主要有感官法、电泳分析法、免疫分析法、PCR法、色谱分析法等,对各种鉴别方法的优缺点进行了分析说明,并对各鉴别方法的应用现状作了简单介绍。
With the advancement in the identification technology of meat products, the identification methods of fake mutton have become more diversified, and the accuracy and efficiency of identification have become higher and higher.In this paper, the advantages and disadvantages of various identification methods, such as sensory method,electrophoresis analysis, immunoassay, PCR, and chromatographic analysis, were analyzed and explained. The application status of these methods was briefly introduced.
引文
[1]BALLIN N Z,VOGENSEN F K,KARLSSON A H.Species determination:can we detect and quantify meat adulteration[J].Meat Science,2009,83(2):165-174.
[2]MAGDALENA M,EDWARD P.Species identification of meat by electrophoretic methods[J].Acta Sc Pol Technol Aliment,2007,6(1):5-16.
[3]SKROKKI AL,HORMI O.Composition of minced meat part B:A survey of commercial ground meat[J].Meat Sci,1994,38(3):503-509.
[4]张小莉,魏玲,李宝明,等.肉制品掺假鉴别技术研究进展[J].食品安全质量检测学报,2014,5(10):3 190-3 194.
[5]刘梦恬.肉制食品中动物源性成分鉴别方法研究新进展[J].科技视界,2015(25):157-158.
[6]王守云,袁明美,封聪,等.肉类掺假鉴别技术研究进展[J].肉类研究,2017,31(4):56-61.
[7]RENCOVA E,SVOBODA I,NECIDOVA I.Identification by ELISA of poultry,horse,kangaroo,and rat muscle specific proteins in heat processed products[J].Veterinarni Medicina,2000,45(12):353-356.
[8]李欣南,关一夫.掺假肉检验技术研究现状[J].食品研究与开发,2016,37(5):189-193.
[9]朱雨薇.肉类掺假检测鉴定技术的研究进展[J].食品工业,2014,35(11):242-247.
[10]DOOLEY JJ,PAINE KE,GARRETT SD,et al.Detection of meat species using TaqMan real-time PCR assays[J].Meat Sci,2004,68(3):431-438.
[11]HOU B,MENG XR,ZHANG LY,et al.Development of a sensitive and specific multiplex PCR method for the simultaneous detection of chicken,duck and goose DNA in meat products[J].Meat Sci,2015,39(101):90-94.
[12]CHOU CC,LIN SP,LEE KM,et al.Fast differentiation of meats from fifteen animal species by liquid chromatography with electrochemical detection using copper nanoparticle plated electrodes[J].J Chromatogr B,2007,846:230-239.
[13]李莹莹,张颖颖,丁小军,等.液相色谱-串联质谱法对羊肉中鸭肉掺假的鉴别[J].食品科学,2016,37(06):204-209.