浮萍休眠体形成过程中的淀粉积累
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摘要
以紫萍NJ016为对象,研究浮萍休眠体形成过程中叶状体、休眠体中的淀粉、叶绿素含量及ADP-葡萄糖焦磷酸化酶(AGPase)、α-淀粉酶、β-淀粉酶活性变化情况。结果表明,浮萍休眠体形成初期,AGPase活性先明显增加后逐渐下降,较未经脱落酸处理的叶状体其活性下降99.8%;浮萍休眠体形成过程中,α、β-淀粉酶活性受到不同程度抑制,α、β-淀粉酶活性与淀粉含量呈极显著负相关(P <0.01),叶绿素a(Chl a)、叶绿素b(Chl b)含量下降明显,叶绿素a/叶绿素b值呈增加趋势。因此,浮萍休眠体形成过程中,主要是通过抑制淀粉的降解进行淀粉积累。
引文
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[3]Tao X,Fang Y,Xiao Y,et al.Comparative transcriptome analysis to investigate the high starch accumulation of duckweed(Landoltia punctata)under nutrient starvation[J].Biotechnology for Biofuels,2013,6(1):72-87.
[4]梁雪,贺锋,徐栋,等.人工湿地植物的功能与选择[J].水生态学杂志,2012,33(1):131-138.
[5]Wang W Q,Messing J.Analysis of ADP-glucose pyrophosphorylase expression during turion formation induced by abscisic acid in Spirodela polyrhiza(greater duckweed)[J].BMC Plant Biology,2012,12(1):5-19.
[6]Ley K L.Appenroth K J.carbohydrate metabolism as a possible physiological modulator of dormancy in turions of Spirodela polyrhiza(L.)Schleiden[J].Plant Science,1997,129(1):1-7.
[7]Appenroth K J,Nickel G.Turion formation in Spirodela polyrhiza:the environmental signals that induce the developmental process in nature[J].Physiologia Plantarum,2010,138(3):312-320.
[8]Chang S M,Yang C C,Sung S C.The cultivation and the nutritional value of Lemnaceae[J].Bulletin Chi Kan,1977(24):19-30.
[9]Cheng J J,Stomp A M.Growing duckweed to recover nutrients from wastewaters and for production of fuel ethanol and animal feed[J].Clean-Soil Air Water,2010,37(1):17-26.
[10]Xu J,Cui W,Cheng J J,et al.Production of high-starch duckweed and its conversion to bioethanol[J].Biosystems Engineering,2011,110(2):67-72.
[11]Cui W,Cheng J J.Growing duckweed for biofuel production:a review[J].Plant Biology,2015,17(S1):16-23.
[12]Sakulsingharoj C,Choi S B,Hwang S K,et al.Engineering starch biosynthesis for increasing rice seed weight:the role of the cytoplasmic ADP-glucose pyrophosphorylase[J].Plant Science,2004,167(6):1323-1333.
[13]Tsai C Y,Nelson O E.Starch-deficient maize mutant lacking adenosine dephosphate glucose pyrophosphorylase activity[J].Science,1966,151(3708):341-343.
[14]Kang G Z,Liu G Q,Peng X Q,et al.Increasing the starch content and grain weight of common wheat by overexpression of the cytosolic AGPase large subunit gene[J].Plant Physiology and Biochemistry,2013,73(6):93-98.
[15]Chen H J,Wang S J.Abscisic acid enhances starch degradation and sugar transport in rice upper leaf sheaths at the post-heading stage[J].Acta Physiologiae Plantarum,2012,34(4):1493-1500.
[16]Zhang L,Zhao H,Gan M Z,et al.Application of simultaneous saccharification and fermentation(SSF)from viscosity reducing of raw sweet potato for bioethanol production at laboratory,pilot and industrial scales[J].Bioresource Technology,2011,102(6):4573-4579.
[17]张宪政.作物生理研究法[M].北京:农业出版社,1992.
[18]Zhao Z,Shi H J,Wang M L,et al.Effect of nitrogen and phosphorus deficiency on transcriptional regulation of genes encoding key enzymes of starch metabolism in duckweed(Landoltia punctata)[J].Plant Physiology and Biochemistry,2015,86(5):72-81.
[19]Nakamura Y,Yuki K,Park S Y,et al.Carbohydrate metabolism in the developing endosperm of rice grains[J].Plant&Cell Physiology,1989,30(6):833-839.
[20]Appenroth K J,Augsten H,Liebermann B,et al.Effects of light quality on amino acid composition of proteins in Wolffia arrhiza(L.)Wimm.using a specially modified bradford method[J].Biochemie Und Physiologie Der Pflanzen,1982,177(3):251-258.
[21]Reid M S,Bieleski R L.Response of spirodela oligorrhiza to phosphorus deficiency[J].Plant Physiology,1970,46(4):609-613.
[22]Lin Y,Tanaka S.Ethanol fermentation from biomass resources:current state and prospects[J].Applied Microbiology and Biotechnology,2006,69(6):627-642.
[23]Akihiro T,Mizuno K,Fujimura T.Gene expression of ADP-glucose pyrophosphorylase and starch contents in cultured cells are cooperatively regulated by sucrose and ABA[J].Plant and Cell Physiology,2005,46(6):937-946.