SBG-DHL与MM-SS检测方法对肠道沙门菌的检测效果比较
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摘要
为研究SBG-DHL与MM-SS两种检测方法对肠道沙门菌的检测效果,选取2017年6月至2018年6月我院采集的100份便检样本为研究对象,分别采用MM-SS和SBG-DHL方法对肠道沙门菌进行检测,对比检测结果。结果显示,便检样本经SBG-DHL检测,其可疑菌落检出率、生化符合率、阳性确认率以及检测敏感率均高于MM-SS检测,差异均具有统计学意义(P<0.05)。结果表明,在肠道沙门菌检测中应用SBG-DHL方法,其检测价值优于MM-SS检测,且其具有敏感性高、易掌握等优点。
In order to investigate the efficacy of SBG-DHL method and MM-SS method in detecting enteric salmonella author selected 100 specimen for scatoscopy as research subjects,detected those specimen by using MM-SS method and SBG-DHL method, finally analyzed both detection results. As results, in the rate of detected suspected colonies,in biochemical match rate,in positive confirmed rate,and in sensitivity rate of detection SBG-DHL method was all significantly higher than MM-SS one( P <0.05).Results show that for detecting enteric salmonella SBG-DHL method is superior to MM-SS one,and has high sensitivity,and easily mastered.
In order to investigate the efficacy of SBG-DHL method and MM-SS method in detecting enteric salmonella author selected 100 specimen for scatoscopy as research subjects,detected those specimen by using MM-SS method and SBG-DHL method, finally analyzed both detection results. As results, in the rate of detected suspected colonies,in biochemical match rate,in positive confirmed rate,and in sensitivity rate of detection SBG-DHL method was all significantly higher than MM-SS one( P <0.05).Results show that for detecting enteric salmonella SBG-DHL method is superior to MM-SS one,and has high sensitivity,and easily mastered.
引文
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[2]宋太平,巩跃生,魏淑娥,等.溃疡性结肠炎1 590例肠道菌群检测结果临床分析[J].中国肛肠病杂志,2004,24(7):36-37.
[3]卢熠川,徐杨,刘宇,等.食品中肠道沙门菌焦磷酸测序方法检测[J].中国公共卫生,2014,30(6):835-838.
[4]刘志冰.两种检测方法对肠道沙门菌的检测结果比较[J].华夏医学,2017,30(3):106-108.
[5]吴集才,张敏,吴建英,等.2009年至2013年景德镇市涉及公共卫生从业人员肠道沙门菌检测结果分析[J].实验与检验医学,2015,33(2):242-244.
[6]魏仲秋,常海岭,李月芳,等.2012-2014年儿童非伤寒沙门菌肠道感染的临床流行病学特征及耐药模式[J].中华儿科杂志,2016,54(7):489-495.
[7]陈明明,王芯,葛美霞.从业人员肠道沙门菌检测结果分析及PCR技术的应用[J].微量元素与健康研究,2017,34(6):43-44,46.
[8]韩毅,孙燕萍,周虹,等.实时荧光定量PCR法与分离培养法检测食品从业人员沙门菌和志贺菌的比较研究[J].中国食品卫生杂志,2015,27(2):132-135.