Vc二步发酵后期补充伴生菌对发酵产古龙酸的影响
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摘要
研究了Vc二步发酵后期补充伴生菌对产酸菌产古龙酸的影响。结果表明,在Vc二步发酵后期,产酸量和产酸速率低时,加入适量伴生菌(巨大芽孢杆菌)培养液或伴生活性物质对产酸菌(普通生酮基古龙酸菌)产酸有明显促进作用,提高了古龙酸产量并缩短了发酵周期。研究结果对查明Vc二步混菌发酵过程及机理、优化发酵后期工艺条件提供了理论依据。
The effects of adding companion strains to fermentation broth at later fermentation on 2-keto-L-gulonic acid production were studied. Results showed that adding suitable amount of companion strain or its active substances at later fermentation could stimulate Ketogulonicigenium vulgare growth and 2-KLG production,and shorten fermentation period. The results of our study provided theoretical basis for illuminating fermentation mechanism and optimizing fermentation process of the two-step mixed fermentation of Vc.
The effects of adding companion strains to fermentation broth at later fermentation on 2-keto-L-gulonic acid production were studied. Results showed that adding suitable amount of companion strain or its active substances at later fermentation could stimulate Ketogulonicigenium vulgare growth and 2-KLG production,and shorten fermentation period. The results of our study provided theoretical basis for illuminating fermentation mechanism and optimizing fermentation process of the two-step mixed fermentation of Vc.
引文
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[3]Mandlaa,Yang WC,Liu CB,Xu H.L-sorbose is not only a substrate for 2-keto-l-gulonic acid production in the artificial microbial ecosystem of two strains mixed fermentation.J Ind Microbiol Biot,2015,42(6):897-904.
[2]吕淑霞,冯树,张忠泽,等.VC二步发酵中伴生菌的作用机制[J].微生物学通报,2001,28(5):10-13.
[3]Mandlaa,Yang WC,Liu CB,Xu H.L-sorbose is not only a substrate for 2-keto-l-gulonic acid production in the artificial microbial ecosystem of two strains mixed fermentation.J Ind Microbiol Biot,2015,42(6):897-904.