香菇摇瓶补料分批发酵的研究
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摘要
以菌丝球数量、菌丝球大小、菌丝体生物量及胞内多糖含量为评价指标,筛选香菇发酵的液体种子培养基和发酵培养基,考察了不同补糖方式对香菇摇瓶补料分批发酵的影响。结果表明,适合香菇发酵的液体种子培养基和发酵培养基(g·L-1)为:葡萄糖10,酵母浸粉20,玉米粉15,磷酸二氢钾0.3,硫酸镁0.15,维生素B10.02。在香菇摇瓶补料分批发酵的第6d和第9d分别补加0.25%葡萄糖,香菇菌丝体生物量达到约847mg·(100mL)-1,胞内多糖含量达到约173mg·(100mL)-1,较不加糖时分别增加了约21.79%和34.29%。补料分批发酵能够提高香菇菌丝体生物量和胞内多糖含量,为香菇多糖大规模工业化生产奠定了基础。
Using mycelium pellet number,mycelium pellet size,mycelium biomass,and intracellular polysaccharide content as evaluation indexes,we screened the liquid seed and fermentation media for the fermentation of Lentinus edodes,and investigated the effect of different glucose-feeding modes on the fed-batch fermentation of Lentinus edodes in shake flask.Results showed that the optimal liquid seed and fermentation media were as follows(g·L-1):glucose 10,yeast extract 20,corn flour 15,KH2 PO40.3,MgSO40.15,and VB10.02.When0.25% of glucose was fed at the 6 th day and 9 th day in the process of fed-batch fermentation of Lentinus edodes in shake flash,the mycelium biomass reached about 847 mg·(100 mL)-1 and the intracellular polysaccharide content reached about 173 mg·(100 mL)-1,which increased by about 21.79%and 34.29%,respectively,compared to those by non-glucose-feeding fermentment.Fed-batch fermentation can improve the mycelium biomass and intracellular polysaccharide content,which lays a foundation for large-scale industrial production of lentinan.
Using mycelium pellet number,mycelium pellet size,mycelium biomass,and intracellular polysaccharide content as evaluation indexes,we screened the liquid seed and fermentation media for the fermentation of Lentinus edodes,and investigated the effect of different glucose-feeding modes on the fed-batch fermentation of Lentinus edodes in shake flask.Results showed that the optimal liquid seed and fermentation media were as follows(g·L-1):glucose 10,yeast extract 20,corn flour 15,KH2 PO40.3,MgSO40.15,and VB10.02.When0.25% of glucose was fed at the 6 th day and 9 th day in the process of fed-batch fermentation of Lentinus edodes in shake flash,the mycelium biomass reached about 847 mg·(100 mL)-1 and the intracellular polysaccharide content reached about 173 mg·(100 mL)-1,which increased by about 21.79%and 34.29%,respectively,compared to those by non-glucose-feeding fermentment.Fed-batch fermentation can improve the mycelium biomass and intracellular polysaccharide content,which lays a foundation for large-scale industrial production of lentinan.
引文
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[3]樊榕,殷慧清,尹文娟,等.香菇六糖的脱保护反应研究及表征[J].化学与生物工程,2013,30(8):61-62.FAN R,YIN H Q,YIN W J,et al.Study on deprotection reaction and characterization of lentinan[J].Chemistry&Bioengineering,2013,30(8):61-62.
[4]BAO H H,SUN L J,ZHU Y,et al.Lentinan produces a robust antidepressant-like effect via enhancing the prefrontal Dectin-1/AMPA receptor signaling pathway[J].Behavioural Brain Research,2017,317:263-271.
[5]ROSELL O'-SOTO E,PARNIAKOV O,DENG Q,et al.Application of non-conventional extraction methods:toward a sustainable and green production of valuable compounds from mushrooms[J].Food Engineering Reviews,2016,8(2):214-234.
[6]郝英利,张燎原,孙建安,等.粘质沙雷氏菌G1利用玉米浆干粉和磷酸氢二铵生产2,3-丁二醇的研究[J].化学与生物工程,2013,30(6):34-38.HAO Y L,ZHANG L Y,SUN J A,et al.Study on 2,3-butanediol production by Serratia marcescens G1 using corn steep liquor powder and ammonium dibasic phosphate[J].Chemistry&Bioengineering,2013,30(6):34-38.
[7]UNREAN P,KHAJEERAM S,LAOTENG A.Systematic optimization of fed-batch simultaneous saccharification and fermentation at high-solid loading based on enzymatic hydrolysis and dynamic metabolic modeling of Saccharomyces cerevisiae[J].Applied Microbiology and Biotechnology,2016,100(5):2459-2470.
[8]NUTARATAT P,SRISUK N,ARUNRATTIYAKORN P,et al.Fed-batch fermentation of indole-3-acetic acid production in stirred tank fermenter by red yeast Rhodosporidium paludigenum[J].Biotechnology and Bioprocess Engineering,2016,21(3):414-421.
[9]胡国元,游慧珍,董兰兰,等.茯苓菌丝体液体培养条件研究[J].武汉工程大学学报,2010,32(7):1-4.HU G Y,YOU H Z,DONG L L,et al.Study on mycelia liquid culture of Poria cocos[J].Journal of Wuhan Institute of Technology,2010,32(7):1-4.
[10]HU G Y,LI W W,WU Y X,et al.The polysaccharide produced from liquid culture of Flammulina velutipes[J].Journal of Huazhong Agricultural University,2004,23(1):26-29.
[11]杨新美.食用菌研究法[M].北京:中国农业出版社,1998:102.YANG X M.Research of Edible Fungi[M].Beijing:China Agriculture Press,1998:102.
[12]赵子鹏,朱薇玲,王小琴,等.放线菌327#的摇瓶补料分批发酵研究[J].中国酿造,2012,31(6):122-125.ZHAO Z P,ZHU W L,WANG X Q,et al.Fed-batch fermentation of Actinomycete 327#in shake flasks[J].China Brewing,2012,31(6):122-125.