新型磁性限进分子印迹复合材料的制备及富集性能
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摘要
采用浸渍热解法制备磁性凹凸棒土(MATP),以磺胺二甲氧嘧啶(SDM)为模板分子,4-乙烯基吡啶(4-VP)为功能单体,采用反相原子转移自由基聚合(RATRP)法在MATP表面包覆印迹聚合物薄膜,得到磁性分子印迹聚合物(MATP-MIPs),最后在MATP-MIPs表面接枝亲水性单体甲基丙烯酸羟乙酯(HEMA),得到新型磁性限进分子印迹复合材料(MATP-RAM-MIPs).采用傅里叶变换红外光谱(FTIR)仪、扫描电子显微镜(SEM)、热重分析(TGA)仪、振动样品磁强计(VSM)和X射线衍射(XRD)仪对材料进行了表征.通过静态水接触角和考马斯亮蓝G-250方法证明材料外表面具有良好的亲水性和排阻蛋白的特性;采用静态和选择性吸附实验证实MATP-RAM-MIPs对SDM具有良好的吸附选择性和较高的吸附容量.将该材料直接用于牛血清中磺胺类药物的富集分离,加标回收率为86. 2%～100. 6%,相对标准偏差为2. 1%～4. 2%,可见该材料在复杂样品前处理中具有良好的实际应用价值.
The magnetic attapulgite( MATP) was preparrd by pyrolysis method. Then,using sulfadimethoxine( SDM) as template molecule,4-vinylpyridine( 4-VP) as functional monomer,the imprinted layer was coated on the surface of modified MATP via reverse atom transfer radical polymerization( RATRP),getting magnetic molecularly imprinted polymers( MATP-MIPs). Finally,novel magnetic restricted access media-molecularly imprinted composites( MATP-RAM-MIPs) was obtained by grafting hydrophilic monomer hydroxyethyl methacrylate( HEMA) onto the surface of MATP-MIPs. The polymer was characterized by means of Fourier transform infrared( FTIR) spectrum, scanning electron microscope( SEM), thermogravimetric analysis( TGA),vibrating sample magnetometer( VSM) and X-ray diffraction( XRD). The hydrophilicity of MATPRAM-MIPs and the ability of exclusion of proteins were studied by the water contact angle and Coomassie brilliant blue G-250 method. The adsorption characters of MATP-RAM-MIPs on SDM were studied by adsorption isothermal and selectivity in detail,and MATP-RAM-MIPs were used as solid phase extraction packing to separate and enrichment of SAs from bovine samples. The spiked recoveries were 86. 2%—100. 6%,and the relative standard deviations( RSD) were 2. 1%—4. 2%,indicating that this material has application value in the pretreatment of complex samples.
The magnetic attapulgite( MATP) was preparrd by pyrolysis method. Then,using sulfadimethoxine( SDM) as template molecule,4-vinylpyridine( 4-VP) as functional monomer,the imprinted layer was coated on the surface of modified MATP via reverse atom transfer radical polymerization( RATRP),getting magnetic molecularly imprinted polymers( MATP-MIPs). Finally,novel magnetic restricted access media-molecularly imprinted composites( MATP-RAM-MIPs) was obtained by grafting hydrophilic monomer hydroxyethyl methacrylate( HEMA) onto the surface of MATP-MIPs. The polymer was characterized by means of Fourier transform infrared( FTIR) spectrum, scanning electron microscope( SEM), thermogravimetric analysis( TGA),vibrating sample magnetometer( VSM) and X-ray diffraction( XRD). The hydrophilicity of MATPRAM-MIPs and the ability of exclusion of proteins were studied by the water contact angle and Coomassie brilliant blue G-250 method. The adsorption characters of MATP-RAM-MIPs on SDM were studied by adsorption isothermal and selectivity in detail,and MATP-RAM-MIPs were used as solid phase extraction packing to separate and enrichment of SAs from bovine samples. The spiked recoveries were 86. 2%—100. 6%,and the relative standard deviations( RSD) were 2. 1%—4. 2%,indicating that this material has application value in the pretreatment of complex samples.
引文
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[2]Segura P.A.,Tremblay P.,Picard P.,Gagnon C.,SauvéS.,J.Agric.Food Chem.,2010,58(3),1442-1446
[3]Guo X.T.,Yin Y.Y.,Yang C.,Qian Z.,Res.Chem.Intermed.,2016,42(7),6423-6435
[4]Li G.J.,Liu C.,Wang D.W.,Ding L.,Chem.Res.Chinese Universities,2018,34(6),893-898
[5]NovákováL.,VlˇckováH.,Anal.Chim.Acta,2009,656(1/2),8-35
[6]He J.,Song L.,Chen S.,Li Y.,Wei H.,Zhao D.,Gu K.,Zhang S.,Food Chemistry,2015,187,331-337
[7]Henrique F.B.,Ribeiro S.M.,Nascimento A.F.,Mauro L.F.,Trac-Trend Anal.Chem.,2015,71(1),9-25
[8]Zhang X.A.,Lin W.F.,Chen S.F.,Xu H.,Gu H.C.,Langmuir,2011,27(22),13669-13674
[9]Ge H.,Huang H.L.,Xu M.,Chem.J.Chinese Universities,2016,37(8),1551-1558(葛昊,黄海龙,徐敏.高等学校化学学报,2016,37(8),1551-1558)
[10]Li X.Q.,Yang Z.,Zhang Q.H.,Li H.M.,Anal.Chim.Acta,2014,807,75-83
[11]Ma F.C.,Li X.,Ren X.H.,Chem.J.Chinese Universities,2017,38(8),1347-1353(马芳晨,李欣,任晓慧.高等学校化学学报,2017,38(8),1347-1353)
[12]Murielle G.,Marie-Pierre C.,Hurtaud-Pessel D.,Verdon E.,J.Chromatography B,2016,1011,145-150
[13]Zhao R.,Li X.,Sun B.L.,Li Y.M.,Li Y.Z.,Wang C.,Chem.Res.Chinese Universities,2017,33(6),986-994
[14]Souza I.D.,Melo L.P.,Jardim I.C.,Monteiro J.C.,Nakano A.M.,Queiroz M.E.,Anal.Chim.Acta,2016,932,49-59
[15]Du B.,Qu T.T.,Chen Z.,Cao X.H.,Han S.P.,Shen G.P.,Wang L.,Talanta,2014,129,465-472
[16]Wang J.,Cormack P.A.G.,Sherrington D.C.,Angew.Chem.Int.Ed.,2003,42(43),5336-5338
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[19]Yue G.Q.,Liu C.,Wang D.Z.,Mater.Res.Bull.,2010,45,1319-1323
[20]Wang J.S.,Matyjaszewski K.,Macromolecule,1995,28,7572-7575
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[22]Du B.B.,Xu Y.,Huang Y.H.,Kang M.,Chinese J.Anal.Chem.,2012,40(12),1871-1876(杜碧柏,许杨,黄运红,康敏.分析化学,2012,40(7),1871-1876)
[23]Hu Y.F.,Wang C.,Li X.D.,Liu L.F.,Electrophoresis,2017,38(19),2462-2467
[24]Huang Y.H.,Xu Y.,Du B.B.,He Q.H.,Cao Y.S.,Chinese J.Anal.Chem.,2012,40(7),1011-1018(黄运红,许杨,杜碧柏,何庆华,曹郁生.分析化学,2012,40(7),1011-1018)
[25]Wei X.B.,Xing X.,Wen Q.,Wu Y.,Wang L.H.,Prog.Nat.Sci.,2017,27(3),374-379
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