一种含D-松醇发酵饮料对2型糖尿病大鼠肝损伤的干预作用
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摘要
研究一种含D-松醇功能成分的混合菌种发酵饮料对STZ诱导2型糖尿病大鼠肝损伤的干预作用。通过酿酒酵母和乳酸菌混合发酵含D-松醇的角豆粉(carob powder)提取液,制备一款含D-松醇功能成分的发酵型低糖饮料。采用高脂高糖饲料喂养SD大鼠4周后腹腔注射STZ建立2型糖尿病模型,将实验大鼠分为正常对照组、模型对照组、阳性对照组、发酵饮料低剂量组、发酵饮料高剂量组。灌胃发酵饮料4周后,测定大鼠空腹血糖(FBG)、肝脏指数、天门冬氨酸氨基转移酶(AST)、血清中丙氨酸氨基转移酶(ALT)含量,同时在光镜下观察各组大鼠肝组织细胞病理形态学改变。结果表明:与正常对照组相比,模型对照组大鼠血清中AST、ALT水平极显著性升高(p<0.01),HE染色显示模型对照组大鼠较正常对照组大鼠肝细胞脂肪变性明显,肝组织结构不清楚,肝细胞排列紊乱。与模型对照组大鼠相比,阳性对照组大鼠及发酵饮料剂量组大鼠血清中AST、ALT水平显著性降低(p<0.05),HE染色显示阳性对照组和发酵饮料剂量组大鼠较模型对照组大鼠肝细胞脂肪变性明显减少,肝细胞排列基本规则。结论:此含D-松醇发酵饮料对2型糖尿病大鼠的肝损伤有一定的干预作用。
This experiment studied the intervention effect of a fermented beverage enriched D-pinitol on liver damage of STZ-induced type 2 diabetic rat. To develop a fermented beverage which was low sugar,the fermentation of Saccharomyces cerevisiae and lactic acid bacteria with the carob powder extract enriched D-pinitol was been stuied. The type 2 diabetic rat were fed enrich-food for 4 weeks before administrated STZ,the SD rats were divided into normal control group,model control group,positive control group,low dose of fermented beverage enriched D-pinitol,high dose of fermented beverage enriched D-pinitol. After 4 weeks of a daily dose of the fermented beverage enriched D-pinitol,some biochemical parameters were measured,which includes FBG,the liver index,ALT,AST test and the pathology morphology change of rat liver tissue cell under research microscope. The Results showed that:Compared with normal control group,the serum AST,ALT level increased extremely significantly(p<0.01),HE staining showed hepatocyte steatosis significantly in model control group rat,liver tissue structure was not clear,the arrangement of liver cell was disordered. Compared with model control group,the serum AST,ALT level decreased significantly(p<0.05),HE staining showed liver steatosis decreased significantly in positive control group and dose group,the arrangement of liver cell was well-regulated.The fermented beverage enriched D-pinitol has the positive intervention effect on liver damage of type 2 diabet-ic rat.
This experiment studied the intervention effect of a fermented beverage enriched D-pinitol on liver damage of STZ-induced type 2 diabetic rat. To develop a fermented beverage which was low sugar,the fermentation of Saccharomyces cerevisiae and lactic acid bacteria with the carob powder extract enriched D-pinitol was been stuied. The type 2 diabetic rat were fed enrich-food for 4 weeks before administrated STZ,the SD rats were divided into normal control group,model control group,positive control group,low dose of fermented beverage enriched D-pinitol,high dose of fermented beverage enriched D-pinitol. After 4 weeks of a daily dose of the fermented beverage enriched D-pinitol,some biochemical parameters were measured,which includes FBG,the liver index,ALT,AST test and the pathology morphology change of rat liver tissue cell under research microscope. The Results showed that:Compared with normal control group,the serum AST,ALT level increased extremely significantly(p<0.01),HE staining showed hepatocyte steatosis significantly in model control group rat,liver tissue structure was not clear,the arrangement of liver cell was disordered. Compared with model control group,the serum AST,ALT level decreased significantly(p<0.05),HE staining showed liver steatosis decreased significantly in positive control group and dose group,the arrangement of liver cell was well-regulated.The fermented beverage enriched D-pinitol has the positive intervention effect on liver damage of type 2 diabet-ic rat.
引文
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[13]张泽生,秦程广,高云峰,等.响应面法优化角豆中D-松醇的提取工艺[J].中国食品添加剂,2016(10):154-159
[14]P K M Anu Geethan,P Stanely Mainzen Prince.Antihyperlipidemic Effect of D-Pinitol on Streptozotocin-Induced Diabetic Wistar Rats[J].Biochem Molecular Toxicology,2008,22(4):220-224
[15]王春田,王莉,石岩.二型糖尿病动物模型制备方法探讨[J].实用中医内科杂志,2011,25(4):27-30
[16]张泽生,张梦娜,高云峰,等.D-松醇对二型糖尿病大鼠PI3K及下游糖原合成基因的调控[J].中国食品添加剂,2015(9):58-65
[17]柴思佳,邓翀,董媛媛.南五味子对2型糖尿病大鼠肝脏保护作用的研究[J].中南药学,2014(8):758-761
[18]Forlani G,Di B P,Mannucci E,et al.Prevalence of elevated liver enzymes in type 2 diabetes mellitus and its association with the metabolic syndrome[J].Endocrinol Invest,2008,31:146-152
[2]Wahren J,Ekberg K.Splanchnic regulation of glucose production[J].Annu Rev Nutr,2007,27:329-345
[3]黄斯瑜,李尔凡.血液标本采集过程对检测结果的影响分析[J].实验与检验医学,2013,3(2):191-192
[4]国家医药管理局中草药情报中心站,植物药有效成分手册[M].北京:人民卫生出版社,1986,835
[5]Liu S C,Chuang S M,Tang C H.D-pinitol inhibits RANKL-induced osteoclastogenesis[J].International Immunopharmacology,2012,12:494-500
[6]张泽生,高云峰,张梦娜,等.D-松醇复配Mn2+对糖尿病ICR小鼠降血糖作用的研究[J].中国食品添加剂,2015(5):55-60
[7]张泽生,秦程广,李雨蒙.D-松醇对STZ诱导2型糖尿病小鼠肝损伤的干预作用[J].中国食品添加剂,2016(12):109-114
[8]VILJOEN B C.The Interaction between Yeasts and Bacteria in Dairy Environments[J].Int J Food Microbiology,2001,69:37-44
[9]ADDIS E,FLEET G H,COX J M,et al.The Growth Properties and Interactions of Yeasts and Bacteria Associated with the Maturation of Camembert and Blue-Veined Cheeses[J].Int J Food Microbiology,2001,69:25-36
[10]Leroi F,Pidoux M.Detection of interactions between yeasts and lactic acid bacteria isolated from sugary kefir grains[J].Journal of Applied Microbiology,2008,74(1):48-53
[11]Roostita R,Fleet G H.The occurrence and growth of yeasts in Camembert and blue-veined cheeses[J].International Journal of Food Microbiology,1996,28(3):393-404
[12]张泽生,董硕,高云峰,等.肌醇、D-手性肌醇对大鼠酒精性脂肪肝作用的研究[J].中国食品添加剂,2014(9):85-90
[13]张泽生,秦程广,高云峰,等.响应面法优化角豆中D-松醇的提取工艺[J].中国食品添加剂,2016(10):154-159
[14]P K M Anu Geethan,P Stanely Mainzen Prince.Antihyperlipidemic Effect of D-Pinitol on Streptozotocin-Induced Diabetic Wistar Rats[J].Biochem Molecular Toxicology,2008,22(4):220-224
[15]王春田,王莉,石岩.二型糖尿病动物模型制备方法探讨[J].实用中医内科杂志,2011,25(4):27-30
[16]张泽生,张梦娜,高云峰,等.D-松醇对二型糖尿病大鼠PI3K及下游糖原合成基因的调控[J].中国食品添加剂,2015(9):58-65
[17]柴思佳,邓翀,董媛媛.南五味子对2型糖尿病大鼠肝脏保护作用的研究[J].中南药学,2014(8):758-761
[18]Forlani G,Di B P,Mannucci E,et al.Prevalence of elevated liver enzymes in type 2 diabetes mellitus and its association with the metabolic syndrome[J].Endocrinol Invest,2008,31:146-152