天然产物抗氧化活性评价方法研究进展
|
摘要
体内氧化应激是诱导多种慢性疾病发生发展的主要机制。目前,人工抗氧化剂大多存在毒性,因而人们越来越重视天然抗氧化剂的研究,目前多种抗氧化活性研究方法用于评价天然产物的抗氧化活性,由于抗氧化反应机理各有不同,研究者应采取多种抗氧化方法综合评价天然产物的抗氧化能力。该文旨在分类总结现有的抗氧化活性研究的方法,阐述其原理与特点,为天然产物抗氧化活性研究及相关领域的研究提供依据。
Oxidative stress in the body is the main mechanism induced a variety of chronic disease development. At present,most of artificial antioxidants are toxic,so more and more attentions are paid to the study of antioxidants in natural products. At present,there are plenty of assessment methods of antioxidant activity used for evaluating the antioxidant activity of natural products. Researchers should adopt various methods based on different reaction mechanism of antioxidant capacity to evaluate the antioxidant activity of natural products comprehensively. The classification,principle and characteristics of assessment methods of antioxidant activity were summarized. This report will provide the reference for the antioxidant activity research of natural product and related fields.
Oxidative stress in the body is the main mechanism induced a variety of chronic disease development. At present,most of artificial antioxidants are toxic,so more and more attentions are paid to the study of antioxidants in natural products. At present,there are plenty of assessment methods of antioxidant activity used for evaluating the antioxidant activity of natural products. Researchers should adopt various methods based on different reaction mechanism of antioxidant capacity to evaluate the antioxidant activity of natural products comprehensively. The classification,principle and characteristics of assessment methods of antioxidant activity were summarized. This report will provide the reference for the antioxidant activity research of natural product and related fields.
引文
[1]Guptal V K,Sharma S K.Plants as natural antioxidants[J].Nat Prod Rad,2006,5(4):326-334.
[2]聂伦,熊尚森,余海忠.襄麦冬不同极性部位粗提物抗氧化性能初步评价[J].山地农业生物学报,2013,32(1):32-34.
[3]陈宁,孙一,刘淑莹.核桃蛋白酶解物的制备及抗氧化活性[J].高等学校化学学报,2013,34(1):72-76.
[4]Jia Z,Tang M,Wu J.The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals[J].Food Chem,1999,64:555-559.
[5]李俊丽,王运强,向长萍.南瓜水溶性多糖的体外抗氧化作用[J].华中农业大学学报,2007,26(2):256-259.
[6]张佳艳,熊建文.多糖抗氧化能力体外测定方法研究进展[J].粮食科技与经济,2013,38(6):26-28.
[7]陈玫,张海德,陈敏,等.几种中药不同溶剂组分的抗氧化活性研究[J].中山大学学报(自然科学版),2006,45(6):131-133.
[8]韩少华,朱靖博,王妍妍.邻苯三酚自氧化法测定抗氧化活性的方法研究[J].中国酿造,2009(6):155-157.
[9]Miller N J,Rice-evams C,Davies M J,et al.A novel method for mearing antioxidant capacity and its application to monitoring the antioxidant status in premature neonates[J].Clin Sci.,1993(84):407-412.
[10]李培源,霍丽妮,苏炜,等.总抗氧化能力检测试剂盒(ABTS)法测定江南星蕨的抗氧化活性[J].中国试验方剂学杂志,2011,17(1):162-164.
[11]Berg R,Haenen G,Berg H,et al.Applicability of an improved Trolox equivalent antioxidant capacity(TEAC)assay for evaluation of antioxidant capacity measurements of mixtures[J].Food Chem,1999(66):511-517.
[12]Summa C,Raposo F C,Mccoury J,et al.Effect of roasting on the radical scavenging activity of cocoa beans[J].Eur Food Res Technol,2006,222:368-375.
[13]Tang Y Z,Liu Z Q.Free-Radical-scavenging Effect of Carbazole Derivatives on DPPH and ABTS radicals[J].J Am Oil Chem Soc.,2007,84(12):1095-1100.
[14]吴朝霞,王媛,张琦,等.改进的DPPH法测定葡萄籽原花青素抗氧化能力研究[J].中国酿造,2008(20):77-79.
[15]Brand-williams W,Cuvelier,M E,Berset C.Use of a free radical method to evaluate antioxidant activity[J].LWT-Food Sci Technol,1995(28):25-30.
[16]刘微微,任虹,曹学丽,等.天然产物抗氧化活性体外评价方法研究进展[J].食品科学,2010,31(17):415-419.
[17]郭长江,杨继军,李云峰,等.FRAP法测定水果不同部分抗氧化活性[J].中国公共卫生,2003,19(7):841-843.
[18]高月红,郑建普,朱春赟,等.抗氧化能力检测方法评估及微型化[J].中国药学杂志,2008,43(24):1863-1866.
[19]Yoo K M,Lee K W,Park J B,et al.Variation in major antioxidants and total antioxidant activity of Yuzu(Citrus junos Sieb ex Tanaka)during maturation and between cultivars[J].J Agr Food Chem,2004,52(19):5907-5913.
[20]Ou B,Huang D,Hampsch-woodill M,et al.Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity(ORAC)and ferric reducing antioxidant power(FRAP)assays:a comparative study[J].J Agr Food Chem,2002,50(11):3122-3128.
[21]Tsao R,Yang R,Xie S,et al.Which polyphenolic compounds contribute to the total antioxidant activities of apple[J].J Agr Food Chem,2005,53(12):4989-4995.
[22]徐贵华,张凤梅,张磊.果蔬食品体外抗氧化方法研究进展[J].食品研究与开发,2010,31(11):215-219.
[23]Sun T,Ho C T.Antioxidant activities of buckwheat extracts[J].Food Chem,2005,90(4):743-749.
[24]Handelman G J,Cao G,Walter M F,et al.Antioxidant capacity of oat(Avena sativa L)extracts.1.inhibition of low-density lipoprotein oxidation and oxygen radical absorbance capacity[J].J Agr Food Chem,1999,47(12):4888-4893.
[25]刘国安,常锦春,乔想金,等.多种植物提取物对DMPD自由基清除作用的研究[J].华中师范大学学报,2015,49(1):102-107.
[26]周才琼,张宝勇.印度人参根提取物体内抗氧化作用研究[J].西南大学学报(自然科学版),2008,30(12):111-115.
[27]罗成,周达,鲁晓翔.天然产物抗氧化机理的研究进展[J].食品农业科技,2009(4):335-339.
[28]王晓宇,杜国荣,李华.抗氧化能力的体外测定方法研究进展[J].食品与生物技术学报,2012,31(3):29-34.
[29]于海宁,沈生荣.铜离子与儿茶素的相互作用//中国茶叶学会第三届海峡两岸茶叶学术研讨会论文集[C].长沙:中国茶叶学会,2003:220-225.
[30]陈全斌,苏小建,沈钟苏.罗汉果叶黄酮抗氧化能力研究[J].食品研究与开发,2006,27(10):189-191.
[31]邹磊,甄少波,宋杨.食品抗氧化能力检测方法的研究进展[J].食品工业科技,2011,32(6):463-466.
[32]Ignasiusradux A,Mary A,Umar S,et al.In vitro antioxidant activity of anthocyanins of black soybean seed coat in human low density lipoprotein(LDL)[J].Food Chem,2009,112(3):659-663.
[33]Wolfe K L,Liu R H.Cellular antioxidant activity(CAA)assay for assessing antioxidants,foods,and dietary supplements[J].J Agr Food Chem,2007,55(22):8896-8907.
[34]马小媛,钱卫平.抗氧化能力评价方法[J].化学进展,2011(8):1737-1746.
[35]Li H,Ma X Y,Dong J,et al.Development of methodology based on the pormation process of gold nanoshells for detecting hydrogen peroxide scavenging activity[J].Anal.Chem,2009,81:8916-8922.
[36]Scampicchio M,Wang J,Blasco A J,et al.Nanoparticle-based assays of antioxidant activity[J].Anal.Chem,2006,78(6):2060-2063.
[37]Wang J,Zhou N,Zhu Z,et al.Detection of flavonoids and assay for their antioxidant activity based on enlargement of gold nanoparticles[J].Anal Bioanal Chem,2007,388(5-6):1199-1205.
[38]Zielinska D,Szawara-nowak D,Zielinski H.Comparison of spectrophotometric and electrochemical methods for the evaluation of the antioxidant capacity of buckwheat products after hydrothermal treatment[J].J Agr Food Chem,2007,55(15):6124-6131.
[39]Roginskv V,Barsukova T,Hsu C F,et al.Chain-breaking antioxidant activity and cyclic voltammetry characterization of polyphenols in a range of green,oolong,and black teas[J].J Agr Food Chem,2003,51(19):5798-5802.
[40]Kilmartin P A,Zou H,Waterhouse,et al.A cyclic voltammetry method suitable for characterizing antioxidant properties of wine and wine phenolics[J].J Agr Food Chem,2001,49(4):1957-1965.
[2]聂伦,熊尚森,余海忠.襄麦冬不同极性部位粗提物抗氧化性能初步评价[J].山地农业生物学报,2013,32(1):32-34.
[3]陈宁,孙一,刘淑莹.核桃蛋白酶解物的制备及抗氧化活性[J].高等学校化学学报,2013,34(1):72-76.
[4]Jia Z,Tang M,Wu J.The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals[J].Food Chem,1999,64:555-559.
[5]李俊丽,王运强,向长萍.南瓜水溶性多糖的体外抗氧化作用[J].华中农业大学学报,2007,26(2):256-259.
[6]张佳艳,熊建文.多糖抗氧化能力体外测定方法研究进展[J].粮食科技与经济,2013,38(6):26-28.
[7]陈玫,张海德,陈敏,等.几种中药不同溶剂组分的抗氧化活性研究[J].中山大学学报(自然科学版),2006,45(6):131-133.
[8]韩少华,朱靖博,王妍妍.邻苯三酚自氧化法测定抗氧化活性的方法研究[J].中国酿造,2009(6):155-157.
[9]Miller N J,Rice-evams C,Davies M J,et al.A novel method for mearing antioxidant capacity and its application to monitoring the antioxidant status in premature neonates[J].Clin Sci.,1993(84):407-412.
[10]李培源,霍丽妮,苏炜,等.总抗氧化能力检测试剂盒(ABTS)法测定江南星蕨的抗氧化活性[J].中国试验方剂学杂志,2011,17(1):162-164.
[11]Berg R,Haenen G,Berg H,et al.Applicability of an improved Trolox equivalent antioxidant capacity(TEAC)assay for evaluation of antioxidant capacity measurements of mixtures[J].Food Chem,1999(66):511-517.
[12]Summa C,Raposo F C,Mccoury J,et al.Effect of roasting on the radical scavenging activity of cocoa beans[J].Eur Food Res Technol,2006,222:368-375.
[13]Tang Y Z,Liu Z Q.Free-Radical-scavenging Effect of Carbazole Derivatives on DPPH and ABTS radicals[J].J Am Oil Chem Soc.,2007,84(12):1095-1100.
[14]吴朝霞,王媛,张琦,等.改进的DPPH法测定葡萄籽原花青素抗氧化能力研究[J].中国酿造,2008(20):77-79.
[15]Brand-williams W,Cuvelier,M E,Berset C.Use of a free radical method to evaluate antioxidant activity[J].LWT-Food Sci Technol,1995(28):25-30.
[16]刘微微,任虹,曹学丽,等.天然产物抗氧化活性体外评价方法研究进展[J].食品科学,2010,31(17):415-419.
[17]郭长江,杨继军,李云峰,等.FRAP法测定水果不同部分抗氧化活性[J].中国公共卫生,2003,19(7):841-843.
[18]高月红,郑建普,朱春赟,等.抗氧化能力检测方法评估及微型化[J].中国药学杂志,2008,43(24):1863-1866.
[19]Yoo K M,Lee K W,Park J B,et al.Variation in major antioxidants and total antioxidant activity of Yuzu(Citrus junos Sieb ex Tanaka)during maturation and between cultivars[J].J Agr Food Chem,2004,52(19):5907-5913.
[20]Ou B,Huang D,Hampsch-woodill M,et al.Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity(ORAC)and ferric reducing antioxidant power(FRAP)assays:a comparative study[J].J Agr Food Chem,2002,50(11):3122-3128.
[21]Tsao R,Yang R,Xie S,et al.Which polyphenolic compounds contribute to the total antioxidant activities of apple[J].J Agr Food Chem,2005,53(12):4989-4995.
[22]徐贵华,张凤梅,张磊.果蔬食品体外抗氧化方法研究进展[J].食品研究与开发,2010,31(11):215-219.
[23]Sun T,Ho C T.Antioxidant activities of buckwheat extracts[J].Food Chem,2005,90(4):743-749.
[24]Handelman G J,Cao G,Walter M F,et al.Antioxidant capacity of oat(Avena sativa L)extracts.1.inhibition of low-density lipoprotein oxidation and oxygen radical absorbance capacity[J].J Agr Food Chem,1999,47(12):4888-4893.
[25]刘国安,常锦春,乔想金,等.多种植物提取物对DMPD自由基清除作用的研究[J].华中师范大学学报,2015,49(1):102-107.
[26]周才琼,张宝勇.印度人参根提取物体内抗氧化作用研究[J].西南大学学报(自然科学版),2008,30(12):111-115.
[27]罗成,周达,鲁晓翔.天然产物抗氧化机理的研究进展[J].食品农业科技,2009(4):335-339.
[28]王晓宇,杜国荣,李华.抗氧化能力的体外测定方法研究进展[J].食品与生物技术学报,2012,31(3):29-34.
[29]于海宁,沈生荣.铜离子与儿茶素的相互作用//中国茶叶学会第三届海峡两岸茶叶学术研讨会论文集[C].长沙:中国茶叶学会,2003:220-225.
[30]陈全斌,苏小建,沈钟苏.罗汉果叶黄酮抗氧化能力研究[J].食品研究与开发,2006,27(10):189-191.
[31]邹磊,甄少波,宋杨.食品抗氧化能力检测方法的研究进展[J].食品工业科技,2011,32(6):463-466.
[32]Ignasiusradux A,Mary A,Umar S,et al.In vitro antioxidant activity of anthocyanins of black soybean seed coat in human low density lipoprotein(LDL)[J].Food Chem,2009,112(3):659-663.
[33]Wolfe K L,Liu R H.Cellular antioxidant activity(CAA)assay for assessing antioxidants,foods,and dietary supplements[J].J Agr Food Chem,2007,55(22):8896-8907.
[34]马小媛,钱卫平.抗氧化能力评价方法[J].化学进展,2011(8):1737-1746.
[35]Li H,Ma X Y,Dong J,et al.Development of methodology based on the pormation process of gold nanoshells for detecting hydrogen peroxide scavenging activity[J].Anal.Chem,2009,81:8916-8922.
[36]Scampicchio M,Wang J,Blasco A J,et al.Nanoparticle-based assays of antioxidant activity[J].Anal.Chem,2006,78(6):2060-2063.
[37]Wang J,Zhou N,Zhu Z,et al.Detection of flavonoids and assay for their antioxidant activity based on enlargement of gold nanoparticles[J].Anal Bioanal Chem,2007,388(5-6):1199-1205.
[38]Zielinska D,Szawara-nowak D,Zielinski H.Comparison of spectrophotometric and electrochemical methods for the evaluation of the antioxidant capacity of buckwheat products after hydrothermal treatment[J].J Agr Food Chem,2007,55(15):6124-6131.
[39]Roginskv V,Barsukova T,Hsu C F,et al.Chain-breaking antioxidant activity and cyclic voltammetry characterization of polyphenols in a range of green,oolong,and black teas[J].J Agr Food Chem,2003,51(19):5798-5802.
[40]Kilmartin P A,Zou H,Waterhouse,et al.A cyclic voltammetry method suitable for characterizing antioxidant properties of wine and wine phenolics[J].J Agr Food Chem,2001,49(4):1957-1965.