牛体外胚胎培养技术研究进展
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摘要
为提高优秀淘汰牛的利用价值,国内外对牛体外胚胎培养技术做了大量研究,本文针对牛体外卵子获得方式、卵子体外成熟培养、成熟卵母细胞与冻精或性控冻精体外受精及受精卵体外培养发育到囊胚等关键技术的研究进展做一综述。
In order to improve the utilization value of culled cattle,agreat deal of studies have been done on bovine embryo culture in vitro.Herein,this review focused on the advances in the key technology,such as in vitro acquisition and maturation of oocytes,in vitro fertilization of mature oocytes and cryopreserved or sexually controlled cryopreserved sperm,and the development of in vitro culture of fertilized eggs to blastocysts.
In order to improve the utilization value of culled cattle,agreat deal of studies have been done on bovine embryo culture in vitro.Herein,this review focused on the advances in the key technology,such as in vitro acquisition and maturation of oocytes,in vitro fertilization of mature oocytes and cryopreserved or sexually controlled cryopreserved sperm,and the development of in vitro culture of fertilized eggs to blastocysts.
引文
[1]许红喜,张智军,邢雪森,吴蒙,张继国,徐仙洲,王忠来,白苏友拉图.雪龙黑牛活体采卵与屠宰采卵在体外受精技术中的应用研究[J].中国奶牛,2013,31(16):12-14.
[2]麻柱.荷斯坦奶牛活体采卵与体外受精技术研究[D].河北农业大学,2006.
[3]A.A.C.De Wit.Effect of Urea During In Vitro Maturation on Nuclear Maturation and Embryo Development of Bovine Cumulus-Oocyte-Complexes[J].Dairy Sci.2001,84:1800-1804.
[4]IR.D.Wilson.in Vitro Production of Holstein Embryos Using Sex-Sorted Sperm and Oocytes from Selected Cull Cows[J].Dairy Sci.2005,88:776-782.
[5]M.Rerat.In Vitro Embryo Production:Growth Performance,Feed Efficiency,and Hematological,Metabolic,and Endocrine Status in Calves[J].Dairy Sci.2005,88:2579-2593.
[6]J.Xu.Developmental Potential of Vitrified Holstein Cattle Embryos Fertilized In Vitro with Sex-Sorted Sperm[J].Dairy Sci.2006,89:2510-2518.
[7]刘新峰,郝志明,史远刚,赵明辉,王敏,李正锋.不同培养体系对牛卵母细胞体外受精的影响[J].安徽农业科学,2011,39(2):1049-1050.
[8]胥焘,窦忠英,陈静波.EGF和GSH对牛卵母细胞体外受精的影响[J].中国农学通报,2005,21(6):55-57.
[9]冯佳时.精子处理方法及β-巯基乙醇对牛体外受精的影响[D].华中农业大学,2011.
[10]禹学礼.培养条件对牛卵母细胞体外受精后胚胎发育的影响[J].西北农林科技大学学报,2005,33(8):16-20.
[11]朱淑文.不同浓度CO2牛卵母细胞体外成熟和受精的影响[J].动物科学与动物医学,2003,20(9):43-44.
[12]李晓霞,曹平华,禹学礼.牛体外发育卵母细胞成熟的评估方法研究[J].河南农业科学,2013,42(7):130-133.
[13]陈善军,梁冠生,张嘉保,王艳军,吴宏,刘健.Percoll法处理牛精子对体外受精胚胎发育的影响[J].中国兽医学报1998,18(4):394-397.
[2]麻柱.荷斯坦奶牛活体采卵与体外受精技术研究[D].河北农业大学,2006.
[3]A.A.C.De Wit.Effect of Urea During In Vitro Maturation on Nuclear Maturation and Embryo Development of Bovine Cumulus-Oocyte-Complexes[J].Dairy Sci.2001,84:1800-1804.
[4]IR.D.Wilson.in Vitro Production of Holstein Embryos Using Sex-Sorted Sperm and Oocytes from Selected Cull Cows[J].Dairy Sci.2005,88:776-782.
[5]M.Rerat.In Vitro Embryo Production:Growth Performance,Feed Efficiency,and Hematological,Metabolic,and Endocrine Status in Calves[J].Dairy Sci.2005,88:2579-2593.
[6]J.Xu.Developmental Potential of Vitrified Holstein Cattle Embryos Fertilized In Vitro with Sex-Sorted Sperm[J].Dairy Sci.2006,89:2510-2518.
[7]刘新峰,郝志明,史远刚,赵明辉,王敏,李正锋.不同培养体系对牛卵母细胞体外受精的影响[J].安徽农业科学,2011,39(2):1049-1050.
[8]胥焘,窦忠英,陈静波.EGF和GSH对牛卵母细胞体外受精的影响[J].中国农学通报,2005,21(6):55-57.
[9]冯佳时.精子处理方法及β-巯基乙醇对牛体外受精的影响[D].华中农业大学,2011.
[10]禹学礼.培养条件对牛卵母细胞体外受精后胚胎发育的影响[J].西北农林科技大学学报,2005,33(8):16-20.
[11]朱淑文.不同浓度CO2牛卵母细胞体外成熟和受精的影响[J].动物科学与动物医学,2003,20(9):43-44.
[12]李晓霞,曹平华,禹学礼.牛体外发育卵母细胞成熟的评估方法研究[J].河南农业科学,2013,42(7):130-133.
[13]陈善军,梁冠生,张嘉保,王艳军,吴宏,刘健.Percoll法处理牛精子对体外受精胚胎发育的影响[J].中国兽医学报1998,18(4):394-397.