伤科黄水澄清工艺研究
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摘要
目的:探讨伤科黄水的最佳澄清工艺,提高制剂澄明度。方法:采用高效液相色谱测定伤科黄水中小檗碱、栀子苷的含量。以小檗碱、栀子苷含量和制剂澄明度为考察指标,先采用过滤、离心和活性炭吸附的方式去除提取液中的固体不溶物,再加入吐温-80和苯甲醇,调节pH值,进行澄清工艺优化。结果:伤科黄水优选的澄清工艺为0.5%活性炭吸附处理后,3 000r/min离心20min,0.45μm滤膜过滤,加1%的吐温-80和0.5%的苯甲醇,调节pH值至6.8保存。结论:本研究优选的伤科黄水澄清工艺稳定、可靠,保留了具有主要药理活性的有效成分。
Objective: To optimize the clarification process of Shangkehuangshui to improve formulation clarity.Methods: HPLC was employed to determine the contents of berberine and geniposide in Shangkehuangshui. Taking clarification effect and the contents of berberine and geniposide in Shangkehuangshui as text indexes,clarification process optimizationuses filtration, centrifugal, activated carbon adsorption, adding amount of twain-80 and benzyl alcohol, and the adjustment of pH value.Results: Optimal clarification process of Shangkehuangshui was 0.5% activated carbon adsorption, 3 000 r/min centrifuged for20 min, filtrated with 0.45μm filter, adding amount of 1% twain-80 and 0.5% benzyl alcohol, and adjusting pH at 6.8 to save.Conclusion: The clarification process of Shangkehuangshui in this study is stable and reliabal, which retain the active ingredient having the main pharmacological activity.
Objective: To optimize the clarification process of Shangkehuangshui to improve formulation clarity.Methods: HPLC was employed to determine the contents of berberine and geniposide in Shangkehuangshui. Taking clarification effect and the contents of berberine and geniposide in Shangkehuangshui as text indexes,clarification process optimizationuses filtration, centrifugal, activated carbon adsorption, adding amount of twain-80 and benzyl alcohol, and the adjustment of pH value.Results: Optimal clarification process of Shangkehuangshui was 0.5% activated carbon adsorption, 3 000 r/min centrifuged for20 min, filtrated with 0.45μm filter, adding amount of 1% twain-80 and 0.5% benzyl alcohol, and adjusting pH at 6.8 to save.Conclusion: The clarification process of Shangkehuangshui in this study is stable and reliabal, which retain the active ingredient having the main pharmacological activity.
引文
[1]蔡薇,廖锡锵.伤科黄水消除胫腓骨双骨折术前肿胀疗效观察.亚太传统医药,2017,13(2):140-141
[2]潘海文,王朝辉,李卓伟,等.伤科黄水预防断指再植术后血管危象的临床观察.中成药,2017,39(5):916-921
[3]罗学辉,谢学文,曾文磊,等.关节镜下有限清理术配合伤科黄水外敷治疗膝骨性关节炎的效果.广东医学,2016,37(7):1078-1081
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[5]崔思娇,杨光照,于士龙,等.软肝缩脾合剂澄清工艺的优化.中成药,2017,39(3):629-631
[6]张建锋,刘文,侯晓杰,等.黄连超高液相色谱-质谱联用血清药物化学研究.时珍国医国药,2017,28(2):284-285
[7]刘洋,隗丽,李雪莲,等.离体法及在体法对黄连水煎液中肠吸收多成分的初步研究.中国中药杂志,2017,42(8):1551-1556
[8]徐姣,赵嵘,代云桃,等.栀子标准汤剂的质量评价方法考察.中国实验方剂学杂志,2017,23(7):30-35
[9]姚超,辛华,陆兔林,等.不同产地栀子的超高效液相色谱指纹图谱及模式识别研究.中国药学杂志,2017,52(1):63-67
[10]肖日传,苗琦,罗光明,等.栀子药材商品规格等级标准研究.中国中药杂志,2017,42(4):663-668
[11]王珏乐,刘文,杨道斌,等.基于灰色关联分析的黄连谱效关系研究.中华中医药杂志,2017,32(12):5583-5586
[2]潘海文,王朝辉,李卓伟,等.伤科黄水预防断指再植术后血管危象的临床观察.中成药,2017,39(5):916-921
[3]罗学辉,谢学文,曾文磊,等.关节镜下有限清理术配合伤科黄水外敷治疗膝骨性关节炎的效果.广东医学,2016,37(7):1078-1081
[4]田晨颖,庞萌萌,李敏,等.宁心合剂的澄清工艺考察.时珍国医国药,2017,28(2):363-365
[5]崔思娇,杨光照,于士龙,等.软肝缩脾合剂澄清工艺的优化.中成药,2017,39(3):629-631
[6]张建锋,刘文,侯晓杰,等.黄连超高液相色谱-质谱联用血清药物化学研究.时珍国医国药,2017,28(2):284-285
[7]刘洋,隗丽,李雪莲,等.离体法及在体法对黄连水煎液中肠吸收多成分的初步研究.中国中药杂志,2017,42(8):1551-1556
[8]徐姣,赵嵘,代云桃,等.栀子标准汤剂的质量评价方法考察.中国实验方剂学杂志,2017,23(7):30-35
[9]姚超,辛华,陆兔林,等.不同产地栀子的超高效液相色谱指纹图谱及模式识别研究.中国药学杂志,2017,52(1):63-67
[10]肖日传,苗琦,罗光明,等.栀子药材商品规格等级标准研究.中国中药杂志,2017,42(4):663-668
[11]王珏乐,刘文,杨道斌,等.基于灰色关联分析的黄连谱效关系研究.中华中医药杂志,2017,32(12):5583-5586