金银花多糖的组成成分与抗氧化活性
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摘要
为金银花多糖的综合开发和利用提供依据,以遵义市绥阳县金银花为试验材料,通过煮提、醇沉,DEAE-纤维素柱和Sepharose CL-6B层析等方法相结合分离纯化金银花多糖(LJP),并采用单因素试验测定金银花多糖各组分对DPPH、羟基自由基和ABTS自由基的清除率,及对H2O2诱导红细胞氧化性溶血的抑制率等体外抗氧化活性。结果表明:金银花中性多糖组分(LJP-N)主要由Glc(43.7%)、Gal(25.1%)和Ara(31.2%)构成,分子量约为6kDa;酸性多糖组分(LJP-A)主要由GalA(82.1%)、Gal(7.1%)和Ara(10.8%)构成,分子量约为400kDa。金银花中性多糖组分和酸性多糖组分均具有一定的体外抗氧化活性,酸性糖组分活性更强。LJP-A和LJP-N对DPPH、羟基自由基及ABTS自由基清除率的EC_(50)分别为0.58mg/mL和2.23mg/mL、3.61mg/mL和>4mg/mL、1.4mg/mL和>4mg/mL,LJP-A和LJP-N对H_2O_2诱导红细胞氧化性溶血的抑制率EC_(50)分别为0.1mg/mL和0.25mg/mL,并且均呈现一定的浓度依赖性。
To provide a basis for the comprehensive development and utilization of Lonicera japonica polysaccharides(LJP),taking L.japonicafrom Suiyang County of Zunyi City as experimental materials,and LJP was separated and purified by combination of digestion,alcohol precipitation,DEAE-cellulose column and Sepharose CL-6 Bchromatography,and a single factor test was employed to determine the clearance rates of various components of LJP on DPPH,hydroxyl radicals and ABTS free radicals,and the inhibition rate of H_2 O_2-induced oxidative hemolysis of red blood cells.Results:The neutral polysaccharide component(LJP-N)of L.japonicaconsisted mainly of Glc(43.7%),Gal(25.1%)and Ara(31.2%),with a molecular weight of about 6 kDa;acidic polysaccharide fraction(LJP-A)was mainly composed of GalA(82.1%),Gal(7.1%)and Ara(10.8%),with a molecular weight of about 400 kDa.Both of LJPN and LJP-A had certain antioxidant activity in vitro,and LJP-A was more active.The EC50 of LJP-A and LJP-N for DPPH,hydroxyl radical and ABTS free radical scavenging rates were 0.58 mg/mL and2.23 mg/mL,3.61 mg/mL and>4 mg/mL,1.4 mg/mL and>4 mg/mL,the EC50 of LJP-A and LJP-N inhibiting H2 O2-induced oxidative hemolysis of red blood cells were 0.1 mg/mL and 0.25 mg/mL,respectively,and they all showed a concentration-dependent manner.
To provide a basis for the comprehensive development and utilization of Lonicera japonica polysaccharides(LJP),taking L.japonicafrom Suiyang County of Zunyi City as experimental materials,and LJP was separated and purified by combination of digestion,alcohol precipitation,DEAE-cellulose column and Sepharose CL-6 Bchromatography,and a single factor test was employed to determine the clearance rates of various components of LJP on DPPH,hydroxyl radicals and ABTS free radicals,and the inhibition rate of H_2 O_2-induced oxidative hemolysis of red blood cells.Results:The neutral polysaccharide component(LJP-N)of L.japonicaconsisted mainly of Glc(43.7%),Gal(25.1%)and Ara(31.2%),with a molecular weight of about 6 kDa;acidic polysaccharide fraction(LJP-A)was mainly composed of GalA(82.1%),Gal(7.1%)and Ara(10.8%),with a molecular weight of about 400 kDa.Both of LJPN and LJP-A had certain antioxidant activity in vitro,and LJP-A was more active.The EC50 of LJP-A and LJP-N for DPPH,hydroxyl radical and ABTS free radical scavenging rates were 0.58 mg/mL and2.23 mg/mL,3.61 mg/mL and>4 mg/mL,1.4 mg/mL and>4 mg/mL,the EC50 of LJP-A and LJP-N inhibiting H2 O2-induced oxidative hemolysis of red blood cells were 0.1 mg/mL and 0.25 mg/mL,respectively,and they all showed a concentration-dependent manner.
引文
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[2]石钺,石任兵,陆蕴如.我国药用金银花资源、化学成分及药理研究进展[J].中国药学杂志,1999,34(11):724-727.
[3]冯卫生,陈欣,郑晓珂,等.金银花化学成分研究[J].中国药学杂志,2011,46(5):338-340.
[4]姜南辉.金银花化学成分研究[J].中药材,2015,38(2):315-317.
[5]宋亚玲,倪付勇,赵祎武,等.金银花化学成分研究进展[J].中草药,2014,45(24):3656-3664.
[6] REN L,PERERA C,HEMAR Y.Antitumor activity of mushroom polysaccharides:a review[J].Food Funct,2012,3(11):1118-1130.
[7] SHRESTHA G,CLAIR L L,O’NEILL K L.The immunostimulating role of Lichen polysaccharides:A Review[J].Phytother Res,2015,29(3):317-322.
[8]刘淑贞,周文果,叶伟建,等.活性多糖的生物活性及构效关系研究进展[J].食品研究与开发,2017,38(18):211-218.
[9]周立,刘裕红,贾俊.复合酶法提取金银花多糖及其抗氧化性[J].山东农业大学学报(自然科学版),2014,5(1):646-650.
[10]向佳兰,王茜,谢阳,等.响应曲面法优化金银花多糖提取条件及抗氧化活性研究[J].乐山师范学院学报(自然科学版),2015,30(12):36-41.
[11]李尔春.金银花多糖的分离纯化与生物活性研究[D].西安:陕西师范大学,2008.
[12]向加林,刘彩珍,杨兰,等.黄秋葵果胶与半乳糖凝集素-3相互作用的研究[J].安徽农业大学学报(自然科学版),2018,45(2):316-320.
[13]周丽梅,詹敏,张涛.对党参多糖的提取、结构及抗氧化活性的分析与研究[J].当代医药论丛,2017,15(17):162-164.
[14]封燕,贡小辉,韦德群,等.金蝉花多糖的抗氧化活性及结构分析[J].食品科学,2016,37(13):19-24.