摘要
为金银花多糖的综合开发和利用提供依据,以遵义市绥阳县金银花为试验材料,通过煮提、醇沉,DEAE-纤维素柱和Sepharose CL-6B层析等方法相结合分离纯化金银花多糖(LJP),并采用单因素试验测定金银花多糖各组分对DPPH、羟基自由基和ABTS自由基的清除率,及对H2O2诱导红细胞氧化性溶血的抑制率等体外抗氧化活性。结果表明:金银花中性多糖组分(LJP-N)主要由Glc(43.7%)、Gal(25.1%)和Ara(31.2%)构成,分子量约为6kDa;酸性多糖组分(LJP-A)主要由GalA(82.1%)、Gal(7.1%)和Ara(10.8%)构成,分子量约为400kDa。金银花中性多糖组分和酸性多糖组分均具有一定的体外抗氧化活性,酸性糖组分活性更强。LJP-A和LJP-N对DPPH、羟基自由基及ABTS自由基清除率的EC_(50)分别为0.58mg/mL和2.23mg/mL、3.61mg/mL和>4mg/mL、1.4mg/mL和>4mg/mL,LJP-A和LJP-N对H_2O_2诱导红细胞氧化性溶血的抑制率EC_(50)分别为0.1mg/mL和0.25mg/mL,并且均呈现一定的浓度依赖性。
To provide a basis for the comprehensive development and utilization of Lonicera japonica polysaccharides(LJP),taking L.japonicafrom Suiyang County of Zunyi City as experimental materials,and LJP was separated and purified by combination of digestion,alcohol precipitation,DEAE-cellulose column and Sepharose CL-6 Bchromatography,and a single factor test was employed to determine the clearance rates of various components of LJP on DPPH,hydroxyl radicals and ABTS free radicals,and the inhibition rate of H_2 O_2-induced oxidative hemolysis of red blood cells.Results:The neutral polysaccharide component(LJP-N)of L.japonicaconsisted mainly of Glc(43.7%),Gal(25.1%)and Ara(31.2%),with a molecular weight of about 6 kDa;acidic polysaccharide fraction(LJP-A)was mainly composed of GalA(82.1%),Gal(7.1%)and Ara(10.8%),with a molecular weight of about 400 kDa.Both of LJPN and LJP-A had certain antioxidant activity in vitro,and LJP-A was more active.The EC50 of LJP-A and LJP-N for DPPH,hydroxyl radical and ABTS free radical scavenging rates were 0.58 mg/mL and2.23 mg/mL,3.61 mg/mL and>4 mg/mL,1.4 mg/mL and>4 mg/mL,the EC50 of LJP-A and LJP-N inhibiting H2 O2-induced oxidative hemolysis of red blood cells were 0.1 mg/mL and 0.25 mg/mL,respectively,and they all showed a concentration-dependent manner.
引文
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