植物激素萘乙酸对诱导石斛芽的影响
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摘要
探索石斛组培时,植物激素萘乙酸(1-naphthylacetic acid NAA)对外植体生长的影响与调节作用,石斛组培时NAA合适浓度范围.试验设置初代培养组和继代培养组,都设置5个萘乙酸浓度梯度,A组NAA1 mg/L;B组NAA0. 75 mg/L; C组NAA0. 5 mg/L; D组NAA0. 25 mg/L; E组NAA0 mg/L即空白对照组.经过一段时间培养试验结果表明,单独加入萘乙酸对石斛初代培养芽点生芽数量影响差异不显著;继代培养丛生芽数量C组最多;外植体生长状况:C组生长量最大,NAA适合浓度0. 5 mg/L.
This experiment is to explore caulis dendrobii seed,plant hormone rootone foreign implant growth and regulation,the influence of caulis dendrobii NAA concentration range appropriate transformation. Test set original culture and successive transfer culture group,is set five naphthalene acetic acid concentration gradient,group A NAA1 mg/L; Group B NAA0. 75 mg/L; Group C NAA0. 5 mg/L; Group D NAA0. 25 mg/L; Group E NAA0 mg/L is blank control group. After a period of time to cultivate the experimental results showed that single naphthalene acetic acid was added into caulis dendrobii original training point of bud bud number difference was not significant; Most transgenerational cultivate multiple shoot clumps number group C,NAA concentration of 0. 5 mg/L.
This experiment is to explore caulis dendrobii seed,plant hormone rootone foreign implant growth and regulation,the influence of caulis dendrobii NAA concentration range appropriate transformation. Test set original culture and successive transfer culture group,is set five naphthalene acetic acid concentration gradient,group A NAA1 mg/L; Group B NAA0. 75 mg/L; Group C NAA0. 5 mg/L; Group D NAA0. 25 mg/L; Group E NAA0 mg/L is blank control group. After a period of time to cultivate the experimental results showed that single naphthalene acetic acid was added into caulis dendrobii original training point of bud bud number difference was not significant; Most transgenerational cultivate multiple shoot clumps number group C,NAA concentration of 0. 5 mg/L.
引文
[1]魏丽芳,于桂芬,冯国宝,等.铁皮石斛组织培养与快速繁殖研究进展[J].安徽农业科学,2013,41(35):13561-13563.
[2]赵天榜,陈志香,陈占宽,等.石斛组织培养与栽培技术研究[J].河南农业大学学报,1994,28(2):128-133.
[3]李进进.铁皮石斛茎段离体初代培养研究[J].作物杂志,2010(1):79-80.
[4]刘仲健,张玉婷,王玉,等.铁皮石斛快速繁殖的研究进展[J].植物科学学报,2011,20(6):763-772.
[5]王康正,高文远.石斛属药用植物研究进展[J].中草药,1997,28(10):633-635.
[6]单芹丽. 3种植物生长调节剂对铁皮石斛种苗生长的影响[J].西南农业学报,2011,24(2):712-715.
[7]陆兵.铁皮石斛组织培养研究进展[J].黑龙江农业科学,2009(2):164-167.
[8]何俊平,涂小云.不同培养基配方对铁皮石斛生根培养的影响[J].江苏农业科学,2013,41(2):57-59.
[9]蒋林,丁平,郑迎冬.添加剂对铁皮石斛组织培养和快速繁殖的影响[J].中药材,2003,26(8):539-541.
[2]赵天榜,陈志香,陈占宽,等.石斛组织培养与栽培技术研究[J].河南农业大学学报,1994,28(2):128-133.
[3]李进进.铁皮石斛茎段离体初代培养研究[J].作物杂志,2010(1):79-80.
[4]刘仲健,张玉婷,王玉,等.铁皮石斛快速繁殖的研究进展[J].植物科学学报,2011,20(6):763-772.
[5]王康正,高文远.石斛属药用植物研究进展[J].中草药,1997,28(10):633-635.
[6]单芹丽. 3种植物生长调节剂对铁皮石斛种苗生长的影响[J].西南农业学报,2011,24(2):712-715.
[7]陆兵.铁皮石斛组织培养研究进展[J].黑龙江农业科学,2009(2):164-167.
[8]何俊平,涂小云.不同培养基配方对铁皮石斛生根培养的影响[J].江苏农业科学,2013,41(2):57-59.
[9]蒋林,丁平,郑迎冬.添加剂对铁皮石斛组织培养和快速繁殖的影响[J].中药材,2003,26(8):539-541.