甘蓝型双低油菜杂交种丰油10号纯度的SSR鉴定
|
摘要
品种纯度影响品种的一致性和稳定性,是品种鉴定的一项重要指标。为建立甘蓝型油菜杂交种丰油10号的SSR标记纯度鉴定方法,以丰油10号及其亲本为材料,利用SSR荧光标记与毛细管电泳相结合的方法,在128对SSR引物中筛选出2对可用于丰油10号纯度鉴定的引物。2对SSR引物对丰油10号的纯度鉴定结果为85.64%,与田间表型纯度鉴定的结果 87.18%相比,纯度值非常接近,说明筛选的SSR标记可用于丰油10号杂交种纯度鉴定。
Variety purity is an important index of variety identification, which indicates the uniformity and stability.In order to establish the hybrid purity identification method of SSR markers for Fengyou No.10 in Brassica napus L.,Fengyou No.10 and its parents were used as experimental materials. Fluorescent SSR markers and capillaryelectrophoresis detection technology were employed to screen the primers for hybrid purity identification of FengyouNo.10 from 128 pairs of SSR primers. Results showed that two pairs of SSR primers for hybrid purity identificationof Fengyou No.10 were obtained. The hybrid purity of Fengyou No.10 detected by the two SSR markers was 85.64%,which was very closed to the field morphological identification result(87.18%), showing that the hybrid purity ofFengyou No.10 can be identified by the screened SSR markers.
Variety purity is an important index of variety identification, which indicates the uniformity and stability.In order to establish the hybrid purity identification method of SSR markers for Fengyou No.10 in Brassica napus L.,Fengyou No.10 and its parents were used as experimental materials. Fluorescent SSR markers and capillaryelectrophoresis detection technology were employed to screen the primers for hybrid purity identification of FengyouNo.10 from 128 pairs of SSR primers. Results showed that two pairs of SSR primers for hybrid purity identificationof Fengyou No.10 were obtained. The hybrid purity of Fengyou No.10 detected by the two SSR markers was 85.64%,which was very closed to the field morphological identification result(87.18%), showing that the hybrid purity ofFengyou No.10 can be identified by the screened SSR markers.
引文
[1]卢虹,徐爱遐. SSR分子标记技术在甘蓝型油菜杂交种陕油16纯度鉴定中的应用.种子,2016,35(6):123-126.
[2]殷艳,廖星,余波,等.我国油菜生产区域布局演变和成因分析.中国油料作物学报,2010,32(1):147-151.
[3]王汉中.我国油菜产业发展的历史回顾与展望.中国油料作物学报,2010,32(2):300-302.
[4]孟倩,董军刚,葛娟,等.甘蓝型油菜‘西农18’种子纯度的SSR鉴定.中国农学通报,2013,29(3):153-156.
[5]王欣怡,艾先涛,王俊铎,等.利用SSR快速鉴定棉花品种真实性和纯度.作物学报,2017,43(10):1565-1572.
[6]苏英.浅谈油菜杂种优势利用与油菜制种中微粉问题解决方案.陕西农业科学,2012(6):181-182,246.
[7]刘子记,曹振木,杨衍.应用分子标记技术检测作物杂交种纯度研究进展.种子,2013,32(6):48-52.
[8]梅眉,陆璐. DNA分子标记技术在农作物种子质量检验中的应用.分子植物育种,2005,3(1):129-134.
[9]梅德圣,李云昌,李英德,等.用脂酶同工酶和AFLP标记鉴定甘蓝型油菜“中油杂2号”的种子纯度.江西农业大学学报,2005,27(1):59-62.
[10]张洪源,谈杰,张敏,等.茄子SSR多态性标记筛选及杂交种纯度鉴定.南方农业学报,2017,48(7):1148-1154.
[11]何俊平,张书芬,王建平,等.甘蓝型油菜育种亲本材料遗传多样性的SSR分析.西南农业学报,2015,28(6):2374-2380.
[12]王同华,陈卫江,李莓,等.利用SSR标记鉴定油菜杂交种沣油737种子纯度的研究.农业开发与装备,2014(1):63-64.
[13]车卓,常宏,张伟,等.利用SSR分子标记鉴定玉米杂交种‘吉祥1号’纯度.中国农学通报,2016,32(18):28-32.
[14]孙海燕,顾雯雯,王淑园,等.利用SSR标记鉴定杂交粳稻‘常优1号’种子纯度.分子植物育种,2014,12(6):1128-1132.
[15]王艳娜,王益奎,李文嘉,等.利用SSR分子标记技术鉴定和分析茄子杂种F1的纯度.南方农业学报,2015,46(9):1551-1556.
[16]柳延涛,段维,王波,等. SSR标记技术鉴定‘新食葵7号’品种纯度的研究.西北农业学报,2017,26(11):1614-1618.
[17]梅德圣,胡琼,李英德,等.杂交油菜品种大地55的遗传基础分析及种子纯度分子鉴定.分子植物育种,2014,12(2):246-253.
[18]史慧娟,赵昭,阳治国,等.甘蓝型油菜均隆油5号特征指纹图谱构建和杂种纯度鉴定.种子,2014,33(8):16-20.
[19]刘平武,周国岭,杨光圣,等.双低甘蓝型油菜杂交种亲本指纹图谱构建和杂交种纯度鉴定.作物学报,2005,31(5):640-646.
[20]兰刚,董军刚,孟倩,等.油菜杂交种合油杂2号纯度鉴定的SSR引物筛选.西北农业学报,2012,21(9):74-78.
[21]沈金雄,陆光远,傅廷栋,等.甘蓝型油菜自交不亲和系杂种纯度鉴定.中国油料作物学报,2004,26(4):12-15.
[22]曹金华,王建平,朱家成,等.双低杂交油菜丰油10号干物质积累及养分吸收规律.中国农学通报,2017,33(34):32-39.
[23]何俊平,张书芬,朱家成,等.氮肥运筹方式对不同肥力土壤油菜产量与经济性状的影响.湖北农业科学,2017,56(23):4489-4492.
[24]何俊平,张书芬,王建平,等.氮磷钾配比对不同肥力土壤油菜产量与经济性状的影响.中国农学通报,2017,33(36):41-46.
[25]李倩,汪端华,杨建国.两个茄子品种纯度SSR鉴定与田间检测分析.分子植物育种,2018,16(1):334-338.
[26]刘国栋,王芙蓉,宫永超,等.棉花品种遗传纯度的SSR分子标记鉴定技术研究.棉花学报,2013,25(5):382-387.
[27]朱美霞,李英芝,王建书,等.利用SSR方法鉴定棉花品种纯度.安徽农业科学,2005,33(11):2010-2016.
[2]殷艳,廖星,余波,等.我国油菜生产区域布局演变和成因分析.中国油料作物学报,2010,32(1):147-151.
[3]王汉中.我国油菜产业发展的历史回顾与展望.中国油料作物学报,2010,32(2):300-302.
[4]孟倩,董军刚,葛娟,等.甘蓝型油菜‘西农18’种子纯度的SSR鉴定.中国农学通报,2013,29(3):153-156.
[5]王欣怡,艾先涛,王俊铎,等.利用SSR快速鉴定棉花品种真实性和纯度.作物学报,2017,43(10):1565-1572.
[6]苏英.浅谈油菜杂种优势利用与油菜制种中微粉问题解决方案.陕西农业科学,2012(6):181-182,246.
[7]刘子记,曹振木,杨衍.应用分子标记技术检测作物杂交种纯度研究进展.种子,2013,32(6):48-52.
[8]梅眉,陆璐. DNA分子标记技术在农作物种子质量检验中的应用.分子植物育种,2005,3(1):129-134.
[9]梅德圣,李云昌,李英德,等.用脂酶同工酶和AFLP标记鉴定甘蓝型油菜“中油杂2号”的种子纯度.江西农业大学学报,2005,27(1):59-62.
[10]张洪源,谈杰,张敏,等.茄子SSR多态性标记筛选及杂交种纯度鉴定.南方农业学报,2017,48(7):1148-1154.
[11]何俊平,张书芬,王建平,等.甘蓝型油菜育种亲本材料遗传多样性的SSR分析.西南农业学报,2015,28(6):2374-2380.
[12]王同华,陈卫江,李莓,等.利用SSR标记鉴定油菜杂交种沣油737种子纯度的研究.农业开发与装备,2014(1):63-64.
[13]车卓,常宏,张伟,等.利用SSR分子标记鉴定玉米杂交种‘吉祥1号’纯度.中国农学通报,2016,32(18):28-32.
[14]孙海燕,顾雯雯,王淑园,等.利用SSR标记鉴定杂交粳稻‘常优1号’种子纯度.分子植物育种,2014,12(6):1128-1132.
[15]王艳娜,王益奎,李文嘉,等.利用SSR分子标记技术鉴定和分析茄子杂种F1的纯度.南方农业学报,2015,46(9):1551-1556.
[16]柳延涛,段维,王波,等. SSR标记技术鉴定‘新食葵7号’品种纯度的研究.西北农业学报,2017,26(11):1614-1618.
[17]梅德圣,胡琼,李英德,等.杂交油菜品种大地55的遗传基础分析及种子纯度分子鉴定.分子植物育种,2014,12(2):246-253.
[18]史慧娟,赵昭,阳治国,等.甘蓝型油菜均隆油5号特征指纹图谱构建和杂种纯度鉴定.种子,2014,33(8):16-20.
[19]刘平武,周国岭,杨光圣,等.双低甘蓝型油菜杂交种亲本指纹图谱构建和杂交种纯度鉴定.作物学报,2005,31(5):640-646.
[20]兰刚,董军刚,孟倩,等.油菜杂交种合油杂2号纯度鉴定的SSR引物筛选.西北农业学报,2012,21(9):74-78.
[21]沈金雄,陆光远,傅廷栋,等.甘蓝型油菜自交不亲和系杂种纯度鉴定.中国油料作物学报,2004,26(4):12-15.
[22]曹金华,王建平,朱家成,等.双低杂交油菜丰油10号干物质积累及养分吸收规律.中国农学通报,2017,33(34):32-39.
[23]何俊平,张书芬,朱家成,等.氮肥运筹方式对不同肥力土壤油菜产量与经济性状的影响.湖北农业科学,2017,56(23):4489-4492.
[24]何俊平,张书芬,王建平,等.氮磷钾配比对不同肥力土壤油菜产量与经济性状的影响.中国农学通报,2017,33(36):41-46.
[25]李倩,汪端华,杨建国.两个茄子品种纯度SSR鉴定与田间检测分析.分子植物育种,2018,16(1):334-338.
[26]刘国栋,王芙蓉,宫永超,等.棉花品种遗传纯度的SSR分子标记鉴定技术研究.棉花学报,2013,25(5):382-387.
[27]朱美霞,李英芝,王建书,等.利用SSR方法鉴定棉花品种纯度.安徽农业科学,2005,33(11):2010-2016.