UPLC-MS/MS法快速分析换锦花中加兰他敏的动态积累变化
|
摘要
本研究以超高效液相色谱-串联四极杆/线性离子阱质谱(UPLC-QTRAP-MS/MS)为基础,建立了1种快速准确检测石蒜属植物中加兰他敏含量的方法。提取液超声波辅助提取离心后,经Waters ACQUITY UPLC BEH C18(150 mm×2.1 mm, 1.7μm)色谱柱分离,以含0.1%甲酸的水溶液和乙腈为流动相进行等度洗脱,利用正离子多反应监测模式(MRM)进行分析,使分析时间大幅缩短,提高分析准确度。在0.5～100μg/m L浓度范围内线性关系良好,相关系数达0.999 9,可在3分钟以内的程序时间内得到检测结果,回收率为95.20%～98.92%,相对标准偏差为1.03%～1.75%,经方法学确认本方法准确可靠。应用此方法测得石蒜属换锦花鳞茎周年不同月份加兰他敏含量介于163.86～2 065.79μg/g干重之间,7月积累最高,加兰他敏积累与生长过程密切相关,总体表现为在休眠期积累,不同的生长时期加兰他敏在换锦花各个器官的分配变化较大。本研究揭示了换锦花加兰他敏的动态积累规律,并为研究加兰他敏的合成代谢调控提供了准确的分析手段。
This work established an efficient analytical method based on ultra-performance liquid chromatographyquadrupole linear ion trap mass spectrometry(UPLC-QTRAP-MS/MS) for rapid determination of galanthamine in Lycoris spp. After ultrasonic-assisted extraction and centrifugation, the sample was separated on a Waters ACQUITY UPLC BEH C18(150 mm×2.1 mm, 1.7 μm) with a mobile phase of acetonitrile-water(0.1% formic acid) at isocratic elution program. The target compounds galanthamine was analyzed in the positive ion multiple reaction monitoring(MRM) mode with shorter time and improved accuracy. As a result, good linearities was obtained in a certain concentration ranges(0.5～100 μg/mL) with correlation coefficients of 0.999 9 within 3 min. The average recoveries were 95.20% ～98.92%, and relative standard deviations were 1.03% ～1.75%. The accuracy and reliability were confirmed by methodology assessment. Using this method, we found that the monthly dynamic variation of galanthamine contents in L. sprengeri ranged from 163.86～2 065.79 μg/g dry weight, and the highest accumulation period was July, indicating that accumulation of galanthamine had close relationship with its growth stage.Generally, galanthamine was highly accumulated at the dormancy stage and its accumulation pattern among different tissues varied a lot during different growth stages. This work showed the dynamic accumulation pattern of galanthamine in Lycoris sprengeri, which could provide a reliable and effective technique for the galanthamine accumulation detection and metabolic regulation in Lycoris spp.
This work established an efficient analytical method based on ultra-performance liquid chromatographyquadrupole linear ion trap mass spectrometry(UPLC-QTRAP-MS/MS) for rapid determination of galanthamine in Lycoris spp. After ultrasonic-assisted extraction and centrifugation, the sample was separated on a Waters ACQUITY UPLC BEH C18(150 mm×2.1 mm, 1.7 μm) with a mobile phase of acetonitrile-water(0.1% formic acid) at isocratic elution program. The target compounds galanthamine was analyzed in the positive ion multiple reaction monitoring(MRM) mode with shorter time and improved accuracy. As a result, good linearities was obtained in a certain concentration ranges(0.5～100 μg/mL) with correlation coefficients of 0.999 9 within 3 min. The average recoveries were 95.20% ～98.92%, and relative standard deviations were 1.03% ～1.75%. The accuracy and reliability were confirmed by methodology assessment. Using this method, we found that the monthly dynamic variation of galanthamine contents in L. sprengeri ranged from 163.86～2 065.79 μg/g dry weight, and the highest accumulation period was July, indicating that accumulation of galanthamine had close relationship with its growth stage.Generally, galanthamine was highly accumulated at the dormancy stage and its accumulation pattern among different tissues varied a lot during different growth stages. This work showed the dynamic accumulation pattern of galanthamine in Lycoris sprengeri, which could provide a reliable and effective technique for the galanthamine accumulation detection and metabolic regulation in Lycoris spp.
引文
Deng C.L.,and Zhou J.,2005,Study on leaf micro-morphological characteristics of Lycoris(Amaryllidaceae),Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),25(2):355-362(邓传良,周坚,2005,石蒜属植物叶微形态特征研究,西北植物学报,25(2):355-362)
Fan H.J.,Luan W.,and Li G.K.,2006,Determination of alkaloids in Lycoris radiata with microwave-assisted extraction coupled with high performance liquid chromatography,Fenxi Ceshi Xuebao(Journal of Instrumental Analysis),25(3):27-30(范华均,栾伟,李攻科,2006,微波辅助提取/HPLC分析石蒜中的生物碱,分析测试学报,25(3):27-30)
He J.P.,Chen F.D.,Chen S.M.,Fang W.M.,Liao H.B.,and Luo H.L.,2009,Transformation of Lycoris longituba agglutinin gene to cut chrysanthemum and identification of aphid resistance in the transgenic plants,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),29(11):2318-2325(何俊平,陈发棣,陈素梅,房伟民,缪恒彬,罗火林,2009石蒜凝集素基因转化切花菊及抗蚜性鉴定,西北植物学报,29(11):2318-2325)
Li M.K.,2012,Establishment of analysis methods of alkaloids and study on biosynthesis of Lycoris radiata alkaloids,Thesis for M.S.,Anhui Agricultural Uni versity,Supervisor Zhang Y.Q.,pp.27-35(李明凯,2012,石蒜生物碱分析方法的建立及其生物合成的研究,硕士学位论文,安徽农业大学,导师:张玉琼,pp.27-35)
Li M.K.,Zhang Y.Q.,Dong Z.R.,Gao C.Y.,Zhong Y.L.,Chen N.,and Wang M.F.,2012,Determination of galanthamine lycoramine and lycorine in Lycoris radiate by high performance liquid chromatography,Fenxi Ceshi Xuebao(Journal of Instrumental Analysis),31(8):957-961(李明凯,张玉琼董召荣,高翠云,仲延龙,陈娜,王梅方,2012,高效液相色谱法测定石蒜中加兰他敏、力可拉敏及石蒜碱3种生物碱,分析测试学报,31(8):957-961)
Li Q.Z.,Zhu F.Y.,Gao X.,Sun Y.,Li S.,Tao Y.,Lo C.,and Liu H.,2014,Young leaf chlorosis 2 encodes the stromal-localized heme oxygenase 2 which is required for normal tetrapyrrole metabolism in rice,Planta,240(4):701-712
Liang L.J.,Jiang Y.M.,Xia B.,Jiang M.M.,Peng F.,He S.L.,and Wang R.,2012,Cloning and prokaryotic expression of LrCOMT from Lycoris radiata,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),32(1):23-28(梁丽建,江玉梅,夏冰,蒋明敏,彭峰,何树兰,汪仁,2012,石蒜儿茶酚氧位甲基转移酶基因克隆与原核表达,西北植物学报,32(1):23-28)
Liu J.X.,Luo Y.Y.,Liu X.H.,Song J.P.,Hua Y.J.,and Wang S.N.,2016,Simultaneous determination of phenolic acids,anthraquinones and flavonoids in Xanthii Herba and Xanthii Fructus by UPLC-QTRAP-MS/MS,Zhipu Xuebao(Journal of Chinese Mass Spectrometry Society),37(6):542-553(刘娟秀,罗益远,刘训红,宋建平,华愉教,王胜男,2016,U-PLC-QTRAP-MS/MS法同时测定苍耳类药材中酚酸、蒽醌及黄酮类成分,质谱学报,37(6):542-553)
Quan M.H.,Ou L.J.,She C.W.,Wu X.J.,Chen D.M.,and Lu J.T.,2012,Photosynthetic characteristics of Lycoris aurea and monthly dynamics of alkaloid contents in its bulbs,African J.Biotech.,11(15):3686-3691
Saliba S.,Ptak A.,and Laurain-mattar D.,2015,4'-O-Methylnorbelladine feeding enhances galanthamine and lycorine production by Leucojum aestivum L.shoot cultures,Engineering in Life Sciences,15(6):640-645
Shao J.,Wang X.J.,and Zhou J.,2010,Starch granule distribution characteristics in Lycoris chinensis bulb,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),30(4):713-718(邵京,王晓静,周坚,2010,中国石蒜鳞茎中淀粉粒的分布特征,西北植物学报,30(4):713-718)
Sun C.S.,Zhu H.,and Peng Z.J.,2011,Study on the content of galanthamine from Lycoris by HPLC,Xiandai Zhongyao Yanjiu Yu Shijian(Research and Practice on Chinese Medicines),(6):30-31(孙长生,朱虹,彭志金,2011,石蒜属植物加兰他敏含量研究,现代中药研究与实践,(6):30-31)
Wang R.,Xu S.,Jiang M.M.,He S.L.,Peng F.,and Xia B.,2014,Physiological response of Lycoris sprengeri and Lycoris chinensis to drought stress,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica,)34(10):2041-2048(汪仁,徐晟,蒋明敏,何树兰,彭峰,夏冰,2014,换锦花和中国石蒜对干旱胁迫的生理响应,西北植物学报,34(10):2041-2048)
Wen Z.Y.,Xiong H.R.,Xiong Y.F.,Li X.,Jiang L.H.,and Zhao M.M.,2014,Ultrasonic extraction of galanthamine from wild Lycoris herb,Zhongguo Yesheng Zhiwu Ziyuan(Chinese Wild Plant Resources),33(1):32-35(文祝友,熊海蓉,熊远福,李霞,蒋丽华,赵明明,2014,超声波法提取野生石蒜中加兰他敏,中国野生植物资源,33(1):32-35)
Wu F.L.,Li A.Z.,and Mao H.F.,2005,Determination of galanthamine in bulb of Lycoris radiate by RP-HPLC,Zhongguo Zhongyao Zazhi(China Journal of Chinese Materia Medica)30(7):523-525(吴芳丽,李爱珍,毛慧芳,2005,RP-HPLC测定石蒜中加兰他敏含量,中国中药杂志,30(7):523-525)
Wu Y.D.,Jiao J.,Jiang J.F.,Zhang Y.,Fan X.C.,Sun H.S.,and Liu C.H.,2017,Optimization of UPLC-MS/MS method for melatonin detection and analysis of melatonin content in grape seedlings under the conditions of salt and drough stress with the optimized method,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),37(8):1657-1663(吴艳迪,焦健,姜建福,张颖,樊秀彩,孙海生,刘崇怀2017,褪黑素UPLC-MS/MS检测方法优化及其对干旱和盐胁迫下葡萄幼苗褪黑素含量的分析,西北植物学报,37(8):1657-1663)
Xie J.,Tan F.,Feng W.,and Chen B.,2007,Advances in studies on classification,identification,medicinal ingredients,and biotechnological application of plants in Lycoris herb,Zhongcaoyao(Chinese Traditional and Herbal Drugs),38(12):1902-1905(谢峻,谈锋,冯巍,陈斌,2007,石蒜属植物分类鉴别,药用成分及生物技术应用研究进展,中草药,38(12)1902-1905)
Xu S.,Jiang Y.L.,Jiang M.M.,Bu D.,Fu J.Y.,Xia B.,and Wang R.,2015,Cloning and expression characteristic analysis of P5CS gene from Lycoris radiata,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),35(10):1925-1933(徐晟,江宜龙,蒋明敏,卜多,傅江燕,夏冰,汪仁,2015石蒜LrP5CS基因的克隆与表达特性分析,西北植物学报,35(10):1925-1933)
Yuan J.H.,2011,Analysis of genetic diversity and inter-specific relationships of Lycoris(Amaryllidaceae)from China by SSR-PCR,Yaredai Zhiwu Kexue(Subtropical Plant Science),40(4):1-7(袁菊红,2011,中国石蒜属遗传多样性与亲缘关系的SSR分析,亚热带植物科学,40(4):1-7)
Yuan J.H.,Peng F.,Feng X.,Sun S.,He S.L.,and Xia B.,2007Inter-specific relationships analysis and classification of Lycoris by HPLC chromatograms,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),27(11):2195-2201(袁菊红,彭峰,冯煦,孙视,何树兰,夏冰,2007,应用HPLC图谱进行石蒜属种间关系与分类研究,西北植物学报,27(11):2195-2201)
Zhang X.N.,Nie J.Y.,Yan Z.,Cheng Y.,and Wang Y.J.,2017,Simultaneous detection of 7 kinds of mycotoxins in apple and its products by UPLC-MS/MS,Fenxi Ceshi Xuebao(Journal of Instrumental Analysis),36(5):588-594(张晓男,聂继云,闫震,程杨,王玉娇,2017,UPLC-MS/MS同时检测苹果及其制品中的7种真菌毒素,分析测试学报,36(5):588-594)
Zheng Y.H.,Li Y.,Peng F.,He S.L.,and Xia B.,2010,Relationships of Lycoris herb.based on rpL36~rpS8,Jiangsu Nongye Kexue(Acta Botanica Boreali-Occidentalia Sinica),3:37-39(郑玉红,李莹,彭峰,何树兰,夏冰,2010,基于rpL36~rpS8序列的石蒜属植物亲缘关系研究,江苏农业科学,3:37-39)
Zhou Y.F.,Wang Y.C.,Li B.,Lu Y.,Zhang Y.,and Li W.M.,2016,Determination of the contents of fraxinellone,obacunone and dictamnine in dictamni cortex of Qinling mountains,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(2):457-461(周亚福,王宇超,黎斌,卢元,张燕,李为民,2016,秦岭终南山白鲜皮梣酮、黄柏酮和白鲜碱含量的测定,基因组学与应用生物学,36(2):457-461)
Zhu W.X.,Zhang L.,Li Z.,Zhang L.,Liu Y.F.,and Yang J.Z.,2016,Determination ofβ-methylphenethylamine and picamilon in health products by ultra-performance liquid chromatography-multiple reaction monitoring and online full scan mass spectrometry,Sepu(Chinese Journal of Chromatography),34(7):681-685(祝伟霞,张莉,李睢,张丽,刘亚风,杨冀州,2016,超高效液相色谱-多反应选择离子监测同步在线全扫描质谱技术测定保健品中的类安非他命和匹卡米隆,色谱,34(7):681-685)
Zhu Z.S.,and Xie S.L.,2008,Germplasm resources,medicinal exploitation of genus Lycoris and its rapid propagation,Jiangxi Lingye Keji(Jiangxi Forestry Science and Technology),(5):41-42(朱重胜,谢树禄,2008,石蒜植物的种质资源,药用开发及其快速繁育,江西林业科技,(5):41-42)
Fan H.J.,Luan W.,and Li G.K.,2006,Determination of alkaloids in Lycoris radiata with microwave-assisted extraction coupled with high performance liquid chromatography,Fenxi Ceshi Xuebao(Journal of Instrumental Analysis),25(3):27-30(范华均,栾伟,李攻科,2006,微波辅助提取/HPLC分析石蒜中的生物碱,分析测试学报,25(3):27-30)
He J.P.,Chen F.D.,Chen S.M.,Fang W.M.,Liao H.B.,and Luo H.L.,2009,Transformation of Lycoris longituba agglutinin gene to cut chrysanthemum and identification of aphid resistance in the transgenic plants,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),29(11):2318-2325(何俊平,陈发棣,陈素梅,房伟民,缪恒彬,罗火林,2009石蒜凝集素基因转化切花菊及抗蚜性鉴定,西北植物学报,29(11):2318-2325)
Li M.K.,2012,Establishment of analysis methods of alkaloids and study on biosynthesis of Lycoris radiata alkaloids,Thesis for M.S.,Anhui Agricultural Uni versity,Supervisor Zhang Y.Q.,pp.27-35(李明凯,2012,石蒜生物碱分析方法的建立及其生物合成的研究,硕士学位论文,安徽农业大学,导师:张玉琼,pp.27-35)
Li M.K.,Zhang Y.Q.,Dong Z.R.,Gao C.Y.,Zhong Y.L.,Chen N.,and Wang M.F.,2012,Determination of galanthamine lycoramine and lycorine in Lycoris radiate by high performance liquid chromatography,Fenxi Ceshi Xuebao(Journal of Instrumental Analysis),31(8):957-961(李明凯,张玉琼董召荣,高翠云,仲延龙,陈娜,王梅方,2012,高效液相色谱法测定石蒜中加兰他敏、力可拉敏及石蒜碱3种生物碱,分析测试学报,31(8):957-961)
Li Q.Z.,Zhu F.Y.,Gao X.,Sun Y.,Li S.,Tao Y.,Lo C.,and Liu H.,2014,Young leaf chlorosis 2 encodes the stromal-localized heme oxygenase 2 which is required for normal tetrapyrrole metabolism in rice,Planta,240(4):701-712
Liang L.J.,Jiang Y.M.,Xia B.,Jiang M.M.,Peng F.,He S.L.,and Wang R.,2012,Cloning and prokaryotic expression of LrCOMT from Lycoris radiata,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),32(1):23-28(梁丽建,江玉梅,夏冰,蒋明敏,彭峰,何树兰,汪仁,2012,石蒜儿茶酚氧位甲基转移酶基因克隆与原核表达,西北植物学报,32(1):23-28)
Liu J.X.,Luo Y.Y.,Liu X.H.,Song J.P.,Hua Y.J.,and Wang S.N.,2016,Simultaneous determination of phenolic acids,anthraquinones and flavonoids in Xanthii Herba and Xanthii Fructus by UPLC-QTRAP-MS/MS,Zhipu Xuebao(Journal of Chinese Mass Spectrometry Society),37(6):542-553(刘娟秀,罗益远,刘训红,宋建平,华愉教,王胜男,2016,U-PLC-QTRAP-MS/MS法同时测定苍耳类药材中酚酸、蒽醌及黄酮类成分,质谱学报,37(6):542-553)
Quan M.H.,Ou L.J.,She C.W.,Wu X.J.,Chen D.M.,and Lu J.T.,2012,Photosynthetic characteristics of Lycoris aurea and monthly dynamics of alkaloid contents in its bulbs,African J.Biotech.,11(15):3686-3691
Saliba S.,Ptak A.,and Laurain-mattar D.,2015,4'-O-Methylnorbelladine feeding enhances galanthamine and lycorine production by Leucojum aestivum L.shoot cultures,Engineering in Life Sciences,15(6):640-645
Shao J.,Wang X.J.,and Zhou J.,2010,Starch granule distribution characteristics in Lycoris chinensis bulb,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),30(4):713-718(邵京,王晓静,周坚,2010,中国石蒜鳞茎中淀粉粒的分布特征,西北植物学报,30(4):713-718)
Sun C.S.,Zhu H.,and Peng Z.J.,2011,Study on the content of galanthamine from Lycoris by HPLC,Xiandai Zhongyao Yanjiu Yu Shijian(Research and Practice on Chinese Medicines),(6):30-31(孙长生,朱虹,彭志金,2011,石蒜属植物加兰他敏含量研究,现代中药研究与实践,(6):30-31)
Wang R.,Xu S.,Jiang M.M.,He S.L.,Peng F.,and Xia B.,2014,Physiological response of Lycoris sprengeri and Lycoris chinensis to drought stress,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica,)34(10):2041-2048(汪仁,徐晟,蒋明敏,何树兰,彭峰,夏冰,2014,换锦花和中国石蒜对干旱胁迫的生理响应,西北植物学报,34(10):2041-2048)
Wen Z.Y.,Xiong H.R.,Xiong Y.F.,Li X.,Jiang L.H.,and Zhao M.M.,2014,Ultrasonic extraction of galanthamine from wild Lycoris herb,Zhongguo Yesheng Zhiwu Ziyuan(Chinese Wild Plant Resources),33(1):32-35(文祝友,熊海蓉,熊远福,李霞,蒋丽华,赵明明,2014,超声波法提取野生石蒜中加兰他敏,中国野生植物资源,33(1):32-35)
Wu F.L.,Li A.Z.,and Mao H.F.,2005,Determination of galanthamine in bulb of Lycoris radiate by RP-HPLC,Zhongguo Zhongyao Zazhi(China Journal of Chinese Materia Medica)30(7):523-525(吴芳丽,李爱珍,毛慧芳,2005,RP-HPLC测定石蒜中加兰他敏含量,中国中药杂志,30(7):523-525)
Wu Y.D.,Jiao J.,Jiang J.F.,Zhang Y.,Fan X.C.,Sun H.S.,and Liu C.H.,2017,Optimization of UPLC-MS/MS method for melatonin detection and analysis of melatonin content in grape seedlings under the conditions of salt and drough stress with the optimized method,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),37(8):1657-1663(吴艳迪,焦健,姜建福,张颖,樊秀彩,孙海生,刘崇怀2017,褪黑素UPLC-MS/MS检测方法优化及其对干旱和盐胁迫下葡萄幼苗褪黑素含量的分析,西北植物学报,37(8):1657-1663)
Xie J.,Tan F.,Feng W.,and Chen B.,2007,Advances in studies on classification,identification,medicinal ingredients,and biotechnological application of plants in Lycoris herb,Zhongcaoyao(Chinese Traditional and Herbal Drugs),38(12):1902-1905(谢峻,谈锋,冯巍,陈斌,2007,石蒜属植物分类鉴别,药用成分及生物技术应用研究进展,中草药,38(12)1902-1905)
Xu S.,Jiang Y.L.,Jiang M.M.,Bu D.,Fu J.Y.,Xia B.,and Wang R.,2015,Cloning and expression characteristic analysis of P5CS gene from Lycoris radiata,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),35(10):1925-1933(徐晟,江宜龙,蒋明敏,卜多,傅江燕,夏冰,汪仁,2015石蒜LrP5CS基因的克隆与表达特性分析,西北植物学报,35(10):1925-1933)
Yuan J.H.,2011,Analysis of genetic diversity and inter-specific relationships of Lycoris(Amaryllidaceae)from China by SSR-PCR,Yaredai Zhiwu Kexue(Subtropical Plant Science),40(4):1-7(袁菊红,2011,中国石蒜属遗传多样性与亲缘关系的SSR分析,亚热带植物科学,40(4):1-7)
Yuan J.H.,Peng F.,Feng X.,Sun S.,He S.L.,and Xia B.,2007Inter-specific relationships analysis and classification of Lycoris by HPLC chromatograms,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),27(11):2195-2201(袁菊红,彭峰,冯煦,孙视,何树兰,夏冰,2007,应用HPLC图谱进行石蒜属种间关系与分类研究,西北植物学报,27(11):2195-2201)
Zhang X.N.,Nie J.Y.,Yan Z.,Cheng Y.,and Wang Y.J.,2017,Simultaneous detection of 7 kinds of mycotoxins in apple and its products by UPLC-MS/MS,Fenxi Ceshi Xuebao(Journal of Instrumental Analysis),36(5):588-594(张晓男,聂继云,闫震,程杨,王玉娇,2017,UPLC-MS/MS同时检测苹果及其制品中的7种真菌毒素,分析测试学报,36(5):588-594)
Zheng Y.H.,Li Y.,Peng F.,He S.L.,and Xia B.,2010,Relationships of Lycoris herb.based on rpL36~rpS8,Jiangsu Nongye Kexue(Acta Botanica Boreali-Occidentalia Sinica),3:37-39(郑玉红,李莹,彭峰,何树兰,夏冰,2010,基于rpL36~rpS8序列的石蒜属植物亲缘关系研究,江苏农业科学,3:37-39)
Zhou Y.F.,Wang Y.C.,Li B.,Lu Y.,Zhang Y.,and Li W.M.,2016,Determination of the contents of fraxinellone,obacunone and dictamnine in dictamni cortex of Qinling mountains,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(2):457-461(周亚福,王宇超,黎斌,卢元,张燕,李为民,2016,秦岭终南山白鲜皮梣酮、黄柏酮和白鲜碱含量的测定,基因组学与应用生物学,36(2):457-461)
Zhu W.X.,Zhang L.,Li Z.,Zhang L.,Liu Y.F.,and Yang J.Z.,2016,Determination ofβ-methylphenethylamine and picamilon in health products by ultra-performance liquid chromatography-multiple reaction monitoring and online full scan mass spectrometry,Sepu(Chinese Journal of Chromatography),34(7):681-685(祝伟霞,张莉,李睢,张丽,刘亚风,杨冀州,2016,超高效液相色谱-多反应选择离子监测同步在线全扫描质谱技术测定保健品中的类安非他命和匹卡米隆,色谱,34(7):681-685)
Zhu Z.S.,and Xie S.L.,2008,Germplasm resources,medicinal exploitation of genus Lycoris and its rapid propagation,Jiangxi Lingye Keji(Jiangxi Forestry Science and Technology),(5):41-42(朱重胜,谢树禄,2008,石蒜植物的种质资源,药用开发及其快速繁育,江西林业科技,(5):41-42)