响应面优化超声提取八月瓜皮果胶工艺
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摘要
超声辅助条件下,研究了八月瓜果皮中果胶提取的预处理条件,考察了p H、超声时间、液料比、提取温度、超声功率对果胶得率和半乳糖醛酸含量的影响,采用响应面分析法对工艺进行优化。结果表明,预处理过程中,粒度40目,灭酶所用蒸馏水体积80 m L;提取时p H为1. 5,料液比1∶13(g/m L),提取时间60 min,温度65℃,超声功率150 W,果胶得率可达8. 924%。提取果胶时间减少了一半,为综合利用资源及利用超声提取八月瓜果皮中果胶提供了一条可行性途径。
The extraction of pectin from Akebia trifoliata pericarp with ultrasonic was studied. The pH,ultrasonic time,solid-liquid ratio,extraction temperature and ultrasonic power were investigated for pectin extraction rate and galactaldehyde,using response surface analysis method to optimize the process. The results showed that the optimal extraction condition were as following: in the pretreatment process,the size of the sample powder was 40 mesh,the volume of distilled water used for the enzyme was 80 m L; pH 1. 5,solid-liquid ratio1∶ 13( g/m L),ultrasound time 60 min,extraction temperature 65 ℃ and ultrasonic power150 W. Under the above conditions,the extracting rate of pectin could reach 8. 924%. The extraction time of pectin was reduced by half,which provided a feasible way for comprehensive utilization of resources and ultrasonic extraction of pectin in Akebia trifoliata pericarp.
The extraction of pectin from Akebia trifoliata pericarp with ultrasonic was studied. The pH,ultrasonic time,solid-liquid ratio,extraction temperature and ultrasonic power were investigated for pectin extraction rate and galactaldehyde,using response surface analysis method to optimize the process. The results showed that the optimal extraction condition were as following: in the pretreatment process,the size of the sample powder was 40 mesh,the volume of distilled water used for the enzyme was 80 m L; pH 1. 5,solid-liquid ratio1∶ 13( g/m L),ultrasound time 60 min,extraction temperature 65 ℃ and ultrasonic power150 W. Under the above conditions,the extracting rate of pectin could reach 8. 924%. The extraction time of pectin was reduced by half,which provided a feasible way for comprehensive utilization of resources and ultrasonic extraction of pectin in Akebia trifoliata pericarp.
引文
[1] Jiang Y,Du Y,Zhu X,et al. Physicochemical and comparative properties of pectins extracted from Akebia trifoliata,var. australis,peel[J]. Carbohydrate Polymers,2012,87(2):1663-1669.
[2] Zou S,Yao X,Zhong C,et al. Genetic analysis of fruit traits and selection of superior clonal lines in Akebia trifoliate,(Lardizabalaceae)[J]. Euphytica,2018,214(7):111.
[3] Xu Q L,Wang J,Dong L M,et al. Two new pentacyclic triterpene saponins from the leaves of Akebia trifoliata[J]. Molecules,2016,21(7):962.
[4]李加兴,吴萍,吴越,等.八月瓜果皮果胶提取工艺优化及其理化特性研究[J].食品工业科技,2016,37(1):275-277.
[5]顾仁勇,杨万根,余佶.响应面优化超临界CO2萃取八月瓜幼果多酚工艺[J].食品科学,2015,36(10):76-80.
[6] Wang J,Ren H,Xu Q L,et al. Antibacterial oleanane-type triterpenoids from pericarps of Akebia trifoliata[J]. Food Chemistry,2015,168:623-629.
[7] Wang J,Xu Q L,Zheng M F,et al. Bioactive 30-noroleanane triterpenes from the pericarps of Akebia trifoliata[J].Molecules,2014,19(4):4301-4312.
[8] Colodel C,Vriesmann L C,Teófilo R F,et al. Extraction of pectin from ponkan(Citrus reticulata Blanco cv. Ponkan)peel:Optimization and structural characterization[J]. International Journal of Biological Macromolecules,2018,117:385-391.
[9]李雪峰,符智荣,欧阳玉祝,等.响应面法优化八月瓜果皮中果胶的酸提取工艺[J].应用化工,2014(12):2198-2201.
[10]赵俭波,姜建辉,秦少伟,等.响应面法优化慕萨莱思酒泥果胶超声辅助提取工艺[J].食品科技,2016(10):171-176.
[11]包立军,柯斧,孙凯,等.优化超声波辅助提取豆腐木叶果胶的工艺及产品性质分析[J].现代食品科技,2017(10):201-207.
[12]应姗姗.火龙果皮中果胶提取及改性研究[D].杭州:浙江大学,2014.
[13]王雅婷.籽瓜皮中果胶的提取及纯化技术研究[D].石河子:石河子大学,2013.
[14]魏秋红,贾娟,李轲.超声波辅助提取脐橙皮果胶的研究[J].食品与发酵科技,2013(2):55-58.
[2] Zou S,Yao X,Zhong C,et al. Genetic analysis of fruit traits and selection of superior clonal lines in Akebia trifoliate,(Lardizabalaceae)[J]. Euphytica,2018,214(7):111.
[3] Xu Q L,Wang J,Dong L M,et al. Two new pentacyclic triterpene saponins from the leaves of Akebia trifoliata[J]. Molecules,2016,21(7):962.
[4]李加兴,吴萍,吴越,等.八月瓜果皮果胶提取工艺优化及其理化特性研究[J].食品工业科技,2016,37(1):275-277.
[5]顾仁勇,杨万根,余佶.响应面优化超临界CO2萃取八月瓜幼果多酚工艺[J].食品科学,2015,36(10):76-80.
[6] Wang J,Ren H,Xu Q L,et al. Antibacterial oleanane-type triterpenoids from pericarps of Akebia trifoliata[J]. Food Chemistry,2015,168:623-629.
[7] Wang J,Xu Q L,Zheng M F,et al. Bioactive 30-noroleanane triterpenes from the pericarps of Akebia trifoliata[J].Molecules,2014,19(4):4301-4312.
[8] Colodel C,Vriesmann L C,Teófilo R F,et al. Extraction of pectin from ponkan(Citrus reticulata Blanco cv. Ponkan)peel:Optimization and structural characterization[J]. International Journal of Biological Macromolecules,2018,117:385-391.
[9]李雪峰,符智荣,欧阳玉祝,等.响应面法优化八月瓜果皮中果胶的酸提取工艺[J].应用化工,2014(12):2198-2201.
[10]赵俭波,姜建辉,秦少伟,等.响应面法优化慕萨莱思酒泥果胶超声辅助提取工艺[J].食品科技,2016(10):171-176.
[11]包立军,柯斧,孙凯,等.优化超声波辅助提取豆腐木叶果胶的工艺及产品性质分析[J].现代食品科技,2017(10):201-207.
[12]应姗姗.火龙果皮中果胶提取及改性研究[D].杭州:浙江大学,2014.
[13]王雅婷.籽瓜皮中果胶的提取及纯化技术研究[D].石河子:石河子大学,2013.
[14]魏秋红,贾娟,李轲.超声波辅助提取脐橙皮果胶的研究[J].食品与发酵科技,2013(2):55-58.