富贵籽茎段诱导腋芽增殖与试管苗培育研究
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摘要
以不同植物激素组合和不同发育阶段的茎段为实验因子,开展富贵籽茎段诱导腋芽生长及其增殖和试管苗培育的实验研究。结果:(1)MS+NAA 0.1 mg·L-1+BA 0.50 mg·L-1的培养基配方较适合于从富贵籽无菌苗和当年萌发的嫩枝茎段诱导腋芽生长与增殖;(2)MS+NAA 0.1 mg·L-1+BA 1.50 mg·L-1的组合配方能较好的诱导富贵籽1年生茎段腋芽生长与增殖。(3)1/2MS+NAA 0.50 mg·L-1+IBA 0.30 mg·L-11的组合配方用于不定芽体诱导生根和培育试管苗的效果最好。
Ardisia crenata is important ornamental and medicinal plants. The experimental effect research of the different combinations of plant hormones on induced growth and proliferation axillary buds of aseptic stems,yearly germinating shoots stems and one-year-old stem of perennial plants were carried out. The results were as follows: The medium formula of MS + NAA 0.1 mg·L-1( the same as below) + BA 0. 50 was suitable for induced growth and proliferation axillary buds of aseptic stems and yearly germinating shoots stems,while the medium formula of MS + NAA 0.10 + BA 1.50 was suitable for induced growth and proliferation axillary buds of 1-year old stems. The combination formula of 1 / 2 MS + NAA 0.50 +IBA 0.30 had best effect for adventitious bud rooting and the cultivation of test tube plantlets.
Ardisia crenata is important ornamental and medicinal plants. The experimental effect research of the different combinations of plant hormones on induced growth and proliferation axillary buds of aseptic stems,yearly germinating shoots stems and one-year-old stem of perennial plants were carried out. The results were as follows: The medium formula of MS + NAA 0.1 mg·L-1( the same as below) + BA 0. 50 was suitable for induced growth and proliferation axillary buds of aseptic stems and yearly germinating shoots stems,while the medium formula of MS + NAA 0.10 + BA 1.50 was suitable for induced growth and proliferation axillary buds of 1-year old stems. The combination formula of 1 / 2 MS + NAA 0.50 +IBA 0.30 had best effect for adventitious bud rooting and the cultivation of test tube plantlets.
引文
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[2]江苏新医学院.中药大辞典:上册[M].上海:上海人民出版社,1977:914.
[3]中国植物志编委会.中国植物志:58卷[M].北京:科学出版社,1979:68.
[4]Piacente S.Constituents of Ardisia Japonica and their in vitro anti-HIV activity[J].J Nat Prod,1996(59):565.
[4]田振华,何燕,骆红梅,等.朱砂根抗炎抗菌作用研究[J].西北药学杂志,1998(3):109-110.
[5]王怀真,何功倍,孙江桥,等.朱砂根三萜总皂甙对子宫的兴奋作用[J].中草药,1988(11):19-20.
[6]余定松,刘腾云.砂根的栽培管理技术[J].中国花卉园艺,2005(22):15.
[7]彭光天,黄上志,黄仲立,等.朱砂根愈伤组织诱导及其岩白菜素含量的测定[J].热带亚热带植物学报,2004(1):51-56.
[8]黄美娟,刘小辉,邓亚玲,等.朱砂根的组织培养和快速繁殖[J].植物生理学通讯,2007(6):1149-1150.
[9]孔祥海,陈小红,邱丰艳,等.富贵籽无菌苗诱导愈伤组织及其优化培养[J].龙岩学院学报,2012(2):56-60.
[10]丁力,孔祥海,邱丰艳,等.富贵籽愈伤组织诱导不定芽的研究[J].龙岩学院学报,2012(5):67-71.
[11]张献龙,唐克轩.植物生物技术[M].北京:科学出版社,2004:61-86.
[12]梁美霞.韭葱愈伤组织和不定芽诱导研究[J].中国农学通报,2010(1):48-50.