4种植物花复合提取液的美白功效及刺激性研究
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摘要
采用醇提法以洋甘菊、橙花、茉莉花、百合花4种植物花为原料制备得到复合提取液,应用小鼠黑色素瘤细胞(B16)增殖率测定、黑色素生成抑制试验、细胞内酪氨酸酶活性测定、体外酪氨酸酶活性抑制试验以及红细胞溶血试验对其美白功效和刺激性进行评估。结果表明,4种植物花复合提取液能显著抑制B16细胞内的黑色素合成和酪氨酸酶活性,且不影响细胞的增殖生长;依ECVAM刺激性分类标准,其分级为无刺激性。复合提取液既安全又高效,可以作为天然美白剂添加到化妆品中。
The complex extract was obtained from 4 kinds of flowers(Chamomile,Orange blossom,Jasmine and Lily) with ethanol as solvent,then its whitening effect and irritation were assessed by several methods including proliferation rate of mouse melanoma cells(B16),the inhibition of melanin formation,the intracellular tyrosinase activity assay,in vitro tyrosinase activity inhibition assay,and erythrocyte hemolysis.The results showed that the complex extract could effectively inhibit both melanin formation and tyrosinase activity in B16 cells without any interference to B16 cells proliferation.Meanwhile,this extract was classified as no irritation substance according to the standard of ECVAM.It was a safe and effective ingredient which would have a good potential in the field of natural whitening cosmetics.
The complex extract was obtained from 4 kinds of flowers(Chamomile,Orange blossom,Jasmine and Lily) with ethanol as solvent,then its whitening effect and irritation were assessed by several methods including proliferation rate of mouse melanoma cells(B16),the inhibition of melanin formation,the intracellular tyrosinase activity assay,in vitro tyrosinase activity inhibition assay,and erythrocyte hemolysis.The results showed that the complex extract could effectively inhibit both melanin formation and tyrosinase activity in B16 cells without any interference to B16 cells proliferation.Meanwhile,this extract was classified as no irritation substance according to the standard of ECVAM.It was a safe and effective ingredient which would have a good potential in the field of natural whitening cosmetics.
引文
[1]陶丽莉,刘洋,吴金昊,等.化妆品美白功效评价方法研究进展[J].日用化学品科学,2015,38(3):15-21.
[2]杨彦松,潘浪胜.洋甘菊中黄酮类成分的分离与结构确定[J].应用化工,2008,37(6):697-698.
[3]黄善松,侯鹏娟,李小兰,等.超临界提取苦橙精油的成分分析[J].湖北农业科学,2016,55(12):3182-3184.
[4]张强,陆玲,李建娜,等.茉莉花丙酮提取物的体外抗氧化作用研究[J].食品工业,2016,37(12):102-104.
[5]冉晓燕,李永忠.百合花的研究概况[J].贵州师范学院学报,2014,30(12):35-37.
[6]刘畅,莫思颖,张怡,等.20种植物醇提物美白作用的体外测试[J].日用化学工业,2013,43(6):450-452.
[7]张目,严泽民,朱少娟,等.几种中药的美白作用研究[J].香料香精化妆品,2009(1):33-36.
[8]杨亚军,林莉,丁家宜,等.天然活性物美白功效的细胞生物学研究[J].日用化学工业,2002,32(3):19-21.
[9]康琰琰,张美英,刑少璟,等.几种天然活性物对黑色素细胞毒性及美白功效的比较[J].日用化学品科学,2005,35(6):361-363.
[10]ANDO H,ITOH A,MISHIMA Y,et al.Correlation between the number of melanosomes,tyrosinase m RNA levels,and tyrosinase activity in cultured murine melanoma cells in response to various melanogenesis regulatory agents[J].Journal of Cellular Physiology,1995,163(3):608-614.
[11]刘晓丹,黄璜,陈清西.苯甲酸对蘑菇酪氨酸酶抑制作用机理的研究[J].厦门大学学报(自然科学版),2003,42(1):102-106.
[12]敖华英,程树军,谈伟君.不同哺乳动物红细胞溶血替代眼刺激试验的比较[J].中国组织工程研究,2012,16(12):2163-2166.
[13]陆泽安,郑赛华,马来记,等.绵羊红细胞溶血试验用于化妆品眼刺激试验的动物替代方法研究[J].日用化学品科学,2013,36(1):23-27.
[14]刘肃,何华红,戚莉莉.红细胞溶血试验检测化妆品眼刺激性的初步研究[J].中国医药科学,2015,5(16):188-190.
[2]杨彦松,潘浪胜.洋甘菊中黄酮类成分的分离与结构确定[J].应用化工,2008,37(6):697-698.
[3]黄善松,侯鹏娟,李小兰,等.超临界提取苦橙精油的成分分析[J].湖北农业科学,2016,55(12):3182-3184.
[4]张强,陆玲,李建娜,等.茉莉花丙酮提取物的体外抗氧化作用研究[J].食品工业,2016,37(12):102-104.
[5]冉晓燕,李永忠.百合花的研究概况[J].贵州师范学院学报,2014,30(12):35-37.
[6]刘畅,莫思颖,张怡,等.20种植物醇提物美白作用的体外测试[J].日用化学工业,2013,43(6):450-452.
[7]张目,严泽民,朱少娟,等.几种中药的美白作用研究[J].香料香精化妆品,2009(1):33-36.
[8]杨亚军,林莉,丁家宜,等.天然活性物美白功效的细胞生物学研究[J].日用化学工业,2002,32(3):19-21.
[9]康琰琰,张美英,刑少璟,等.几种天然活性物对黑色素细胞毒性及美白功效的比较[J].日用化学品科学,2005,35(6):361-363.
[10]ANDO H,ITOH A,MISHIMA Y,et al.Correlation between the number of melanosomes,tyrosinase m RNA levels,and tyrosinase activity in cultured murine melanoma cells in response to various melanogenesis regulatory agents[J].Journal of Cellular Physiology,1995,163(3):608-614.
[11]刘晓丹,黄璜,陈清西.苯甲酸对蘑菇酪氨酸酶抑制作用机理的研究[J].厦门大学学报(自然科学版),2003,42(1):102-106.
[12]敖华英,程树军,谈伟君.不同哺乳动物红细胞溶血替代眼刺激试验的比较[J].中国组织工程研究,2012,16(12):2163-2166.
[13]陆泽安,郑赛华,马来记,等.绵羊红细胞溶血试验用于化妆品眼刺激试验的动物替代方法研究[J].日用化学品科学,2013,36(1):23-27.
[14]刘肃,何华红,戚莉莉.红细胞溶血试验检测化妆品眼刺激性的初步研究[J].中国医药科学,2015,5(16):188-190.