5种细菌对苹果轮纹病菌菌丝的拮抗作用
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摘要
作者在前期试验的基础上筛选出5种(茎1、茎2、茎4、花2、姜4)对苹果轮纹病菌菌丝有明显抑制作用的内生细菌,并通过与苹果轮纹病的对峙培养试验,对其进一步的筛选。结果表明,对苹果轮纹病菌菌丝生长抑制效果最好的为从黄顶菊内分离出的花2,其次为从姜内分离的姜4和从黄顶菊内分离的茎2,抑制效果最差的则为从黄顶菊内分离的茎1。
Five endophytic bacteria(stem 1,stem 2,stem 4,flower 2 and ginger 4)were screened out to inhibit the mycelia of Botryosphaeria dothidea Nose,and further screened by confrontation culture with Botryosphaeria dothidea Nose.The results show that,the best inhibiting effect on Botryosphaeria dothidea Nose is the flower 2 isolated from Flaveriabidentis,the second is the ginger 4 isolated from the ginger and the stem 2 isolated from Flaveriabidentis,and the worst is the stem 1 isolated from Flaveriabidentis.
Five endophytic bacteria(stem 1,stem 2,stem 4,flower 2 and ginger 4)were screened out to inhibit the mycelia of Botryosphaeria dothidea Nose,and further screened by confrontation culture with Botryosphaeria dothidea Nose.The results show that,the best inhibiting effect on Botryosphaeria dothidea Nose is the flower 2 isolated from Flaveriabidentis,the second is the ginger 4 isolated from the ginger and the stem 2 isolated from Flaveriabidentis,and the worst is the stem 1 isolated from Flaveriabidentis.
引文
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[2]王海明,邓德法,王惠玉.浅谈生物防治技术在苹果病害防治中的应用[J].落叶果树,2014,46(2):22-23.
[3]周寒.杀菌剂和生防菌协调控制苹果采后轮纹病及作用机制[D].合肥:安徽农业大学,2009.
[4]Ligin JM,Hill DS,Hammer PE,et al.Genetic modification of Pseudomonas that enhance biological disease control[J].Acta Horticulture,1999,54:53-60.
[5]侯晓杰,梁魁景,丛日征,等.贮藏期苹果轮纹病拮抗菌的筛选研究[J].北方园艺,2015(23):115-119.
[6]曹克强,国立耘,李保华,等.中国苹果树腐烂病发生和防治情况调查[J].植物保护,2009,35(2):114-116.