茶树菇复合群系统类群及交配分析
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摘要
茶树菇复合群是一类具有重要经济价值的菌类,其菌株资源多样,命名混乱。为给育种工作提供参考依据,利用线粒体小亚基V4-6-9区序列和交配,对4个代表菌株进行分子和交配特征分析。结果表明:(1)线粒体小亚基序列作为类群区分的有效标记,有效区分杨柳田头菇类群、茶树菇/茶薪菇类群和杨树菇类群;(2)交配特征显示杨柳田头菇为独立生物学种,而其他类群属同一生物学种,建议使用茶树菇这一名称。研究结果可为茶树菇资源鉴定及育种研究提供参考。
Agrocybe aegerita complex, an important mushroom with high economic value, includes many groups with high diversity and confusing denomination. To provide a scientific reference for the breeding work,molecular data of mitochondrial small subunit(mt SSU) V4-6-9 regions and mating tests were employed for the investigation on four reference strains with different designations. The results showed that:(1) mt SSU V4-6-9 could be used as the efficient molecular mark for distinguishing A. salicacola, A. aegerita/A. chaxingu and"Yangshugu"(with Chinese name) groups;(2) mating tests indicated that A. salicacola was an independent biological species, and other groups were the same biological species, A. aegerita was suggested as the general name. The results could provide a reference for the identification and breeding of A. aegerita complex.
Agrocybe aegerita complex, an important mushroom with high economic value, includes many groups with high diversity and confusing denomination. To provide a scientific reference for the breeding work,molecular data of mitochondrial small subunit(mt SSU) V4-6-9 regions and mating tests were employed for the investigation on four reference strains with different designations. The results showed that:(1) mt SSU V4-6-9 could be used as the efficient molecular mark for distinguishing A. salicacola, A. aegerita/A. chaxingu and"Yangshugu"(with Chinese name) groups;(2) mating tests indicated that A. salicacola was an independent biological species, and other groups were the same biological species, A. aegerita was suggested as the general name. The results could provide a reference for the identification and breeding of A. aegerita complex.
引文
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[20]王谦,黄媛媛,宋长林.常压室温等离子体(ARTP)诱变技术选育柱状田头菇(茶树菇)优良菌株研究初探[J].菌物学报,2017,36(6):776-783.
[21]张亦诚,雷朝云,何成勇.杨树菇引种栽培试验初报[J].贵州农业科学,2005,33(1):69.
[22]缪静,孙磊,李维焕,程显好.茶树菇菌种固体培养基及培养条件的优化[J].食用菌,2017,3:35-37.
[23]王洪秀,魏云辉,靳亮,等.基于ITS序列分析18个茶树菇菌株的亲缘关系[J].西南农业学报,2016,29(9):2038-2044.
[24]姜性坚,尹永刚,王小艳,等.湖南地区柱状田头菇的AFLP分析[J].食用菌学报,2013,20(1):25-30.
[25]鲍大鹏,王南,陈明杰,等.采用ARDRA和RAPD对柳松菇(Agrocybe aegerita)菌株遗传多样性的分析[J].上海农业学报,2001,17(1):18-22.
[26]谭琦,严培兰,詹才新,等.利用RAPD技术对不同地理环境下柳松菇菌株亲缘关系的分析[J].上海农业学报,1999,15(4):18-21.
[2]王波.几种食用菌品种名称订正[J].中国食用菌,2006,25(5):27-28.
[3]泽章三,张振东.柱状田头菇的栽培技术[J].国外农学:蚕业,1989(1):6-7.
[4]Uhart M,Piscera J M,AlbertóE.Utilization of new naturally occurring strains and supplementation to improve the biological efficiency of the edible mushroom Agrocybe cylindracea[J].Journal of Industrial Microbiology&Biotechnology,2008,35(6):595-602.
[5]Sharma V P,Sharma S R.Molecular identification and cultivation of the black poplar culinary-medicinal mushroom Agrocybe aegerita(V.Brig.)Singer(Agaricomycetideae)[J].International Journal of Medicinal Mushrooms,2009,11(1):87-91.
[6]张金霞,陈强,黄晨阳,等.食用菌产业发展历史、现状与趋势[J].菌物学报,2015,34(4):524-540.
[7]Uhart M,Sirandpugnet P,Labarère J.Evolution of mitochondrial SSU-r DNA variable domain sequences and r RNA secondary structures,and phylogeny of the Agrocybe aegerita multispecies complex[J].Research in Microbiology,2007,158(3):203.
[8]Uhart M,AlbertóE.Mating tests in Agrocybe cylindracea sensu lato.Recognition of Agrocybe wrightii as a novel species[J].Mycological Progress,2009,8(4):337-349.
[9]Chen W M,Chai H M,Zhou H M,et al.Phylogenetic analysis of the Agrocybe aegerita,multispecies complex in Southwest China inferred from ITS and mt SSU r DNA sequences and mating tests[J].Annals of Microbiology,2012,62(4):1791-1801.
[10]陈卫民,张小雷,柴红梅,等.基于线粒体序列特征的茶树菇及杨柳田头菇分离物快速鉴定[J].中国农学通报,2011,27(31):152-155.
[11]李玉.市场上常见食用菌学名异名探究[J].食药用菌,2013(5):259-262.
[12]李晶,韩冲,陈青君,等.1株野生茶树菇的鉴定及其母种培养条件研究[J].中国农学通报,2015,31(25):70-74.
[13]鲍大鹏,王南,谭琦,等.柱状田头菇的栽培生理及生活史研究概述[J].食用菌学报,1999,6(4):55-60.
[14]张金霞,黄晨阳.北方夏季栽培茶薪菇[J].中国土壤与肥料,2003(4):44-45.
[15]舒丽,陈强,陈翠萍,等.野生茶树菇YW-2生物学特性及驯化栽培[J].北方园艺,2007(8):208-210.
[16]舒丽,陈强,曾树,等.野生茶树菇YW-2液体发酵及子实体氨基酸成分分析[J].西南农业学报,2009,22(4):1033-1037.
[17]柴新义,王四宝,樊美珍.杨树菇深层液体发酵培养基及培养条件的优化[J].西北农林科技大学学报:自然版,2006,34(11):117-12.
[18]王镭,张婤.柱状田头菇(Agrocybe cylindracea)营养菌丝的生物学特性[J].上海农业学报,1993,9:23-28.
[19]吕明亮,胡美芳,应国华.柱状田头菇菌丝营养特性研究[J].食用菌,2003,25:6-7.
[20]王谦,黄媛媛,宋长林.常压室温等离子体(ARTP)诱变技术选育柱状田头菇(茶树菇)优良菌株研究初探[J].菌物学报,2017,36(6):776-783.
[21]张亦诚,雷朝云,何成勇.杨树菇引种栽培试验初报[J].贵州农业科学,2005,33(1):69.
[22]缪静,孙磊,李维焕,程显好.茶树菇菌种固体培养基及培养条件的优化[J].食用菌,2017,3:35-37.
[23]王洪秀,魏云辉,靳亮,等.基于ITS序列分析18个茶树菇菌株的亲缘关系[J].西南农业学报,2016,29(9):2038-2044.
[24]姜性坚,尹永刚,王小艳,等.湖南地区柱状田头菇的AFLP分析[J].食用菌学报,2013,20(1):25-30.
[25]鲍大鹏,王南,陈明杰,等.采用ARDRA和RAPD对柳松菇(Agrocybe aegerita)菌株遗传多样性的分析[J].上海农业学报,2001,17(1):18-22.
[26]谭琦,严培兰,詹才新,等.利用RAPD技术对不同地理环境下柳松菇菌株亲缘关系的分析[J].上海农业学报,1999,15(4):18-21.