望江南子总蒽醌提取工艺优化及DPPH·自由基清除实验研究
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摘要
目的:优化望江南子总蒽醌的提取工艺,并评价其抗氧化活性。方法:采用乙醇提取望江南子总蒽醌,单因素试验考察各因素水平,正交试验优化望江南子总蒽醌的提取工艺,DPPH法评价其抗氧化活性。结果:望江南子总蒽醌最佳提取工艺为乙醇浓度70%,乙醇倍数14倍,提取温度70℃,提取时间1 h,提取次数2次。望江南子总蒽醌可清除DPPH·自由基,且清除率随总蒽醌量的增加而增大。结论:传统醇提法提取望江南子总蒽醌工艺可行,重复性较好。望江南子总蒽醌具有良好的抗氧化活性,可为望江南子的进一步开发提供实验依据。
Objective: To optimize extraction process of anthraquinone from Cassia occidentalis L. and evaluate its antioxidant activity. Methods: Total anthraquinone was extracted by alcohol. Factors and levels were investigated by single factor test. The extraction process of anthraquinone from Cassia occidentalis L. was optimized by orthogonal test. Antioxidant activity was evaluated by DPPH method. Results: The optimum extraction process of anthraquinone from Cassia occidentalis L. is using 14 times 70% alcohol to extract at 70℃ for 1 h and 2 times. Total anthraquinone from Cassia occidentalis L. can scavenge free radical,and scavenging rate increases with total anthraquinone amount. Conclusion: The traditional alcohol extraction method of total anthraquinone from Cassia occidentalis L. is feasible,which also have good repeatability. Total anthraquinone from Cassia occidentalis L. has good antioxidant activity. All above can provide experiment basis for further development for Cassia occidentalis L..
Objective: To optimize extraction process of anthraquinone from Cassia occidentalis L. and evaluate its antioxidant activity. Methods: Total anthraquinone was extracted by alcohol. Factors and levels were investigated by single factor test. The extraction process of anthraquinone from Cassia occidentalis L. was optimized by orthogonal test. Antioxidant activity was evaluated by DPPH method. Results: The optimum extraction process of anthraquinone from Cassia occidentalis L. is using 14 times 70% alcohol to extract at 70℃ for 1 h and 2 times. Total anthraquinone from Cassia occidentalis L. can scavenge free radical,and scavenging rate increases with total anthraquinone amount. Conclusion: The traditional alcohol extraction method of total anthraquinone from Cassia occidentalis L. is feasible,which also have good repeatability. Total anthraquinone from Cassia occidentalis L. has good antioxidant activity. All above can provide experiment basis for further development for Cassia occidentalis L..
引文
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[13]黎海彬,方昆阳,李续娥.中药决明子蒽醌类成分含量测定的研究[J].食品科学,2007,28(7):427-429.
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[15]卓玛莉,陈祺松,何文跃.响应面法优化虎杖总游离蒽醌提取工艺的研究[J].中华中医药学刊,2015,33(8):2017-2020.
[16]许波,窦志华,卞理,等.多指标综合加权评分法优选大黄药材中蒽醌类成分提取工艺[J].中南药学,2016,14(4):370-374.
[2]刘亚菲,王明,巩江,等.望江南药学研究概况[J].辽宁中医药大学学报,2011,13(5):68-70.
[3]Yadav J P,Vedpriya A,Sanjay Y,et al.Cassia occidentalis L.:a review on its ethnobotany,phytochemical and pharmacological profile[J].Fitoterapia,2010,81:223-230.
[4]Li Shifei,Di Yingtong,Wang Yuehu,et al.Anthraquinones and Lignans from Cassia occidentalis[J].Helvetica Chinica Acta,2010,93:1795-1802.
[5]尹宏权,魏洁,尚贝贝,等.望江南化学成分分离和结构鉴定[J].北京理工大学学报,2013,33(10):1098-1100.
[6]黎明,王巧荣,刘建华,等.望江南子挥发性成分的GC-MS分析[J].中国实验方剂学杂志,2013,19(19):122-126.
[7]杜洪志,农亨,董立莎,等.黔产苗药“薄丈达”中不同极性组分DPPH自由基清除活性的研究[J].辽宁中医杂志,2015,40(12):2449-2454.
[8]张霜,李燕.响应曲面法优化芦荟蒽醌提取工艺的研究[J].食品工业科技,2012,33(8):326-329.
[9]邓佳,刘学文.微波法提取决明子中蒽醌工艺条件的优化研究[J].食品研究与开发,2006,27(9):35-37.
[10]高岐.微波提取何首乌中蒽醌含量的应用研究[J].食品研究与开发,2015,36(17):81-82,102.
[11]黄小梅,邓祥,吴狄.何首乌中蒽醌类物质的超声波提取工艺优化[J].化学研究,2015,26(2):166-169.
[12]谢伟雪,刘孝敏,谢伟敬,等.回流法提取大黄总蒽醌类成分的工艺研究[J].中国中医药咨讯,2010,2(29):1-3.
[13]黎海彬,方昆阳,李续娥.中药决明子蒽醌类成分含量测定的研究[J].食品科学,2007,28(7):427-429.
[14]曹亮.虎杖中白藜芦醇和蒽醌类提取分离工艺研究[D].汉中:陕西理工学院,2010.
[15]卓玛莉,陈祺松,何文跃.响应面法优化虎杖总游离蒽醌提取工艺的研究[J].中华中医药学刊,2015,33(8):2017-2020.
[16]许波,窦志华,卞理,等.多指标综合加权评分法优选大黄药材中蒽醌类成分提取工艺[J].中南药学,2016,14(4):370-374.