麦类作物花药离体培养特性遗传控制研究进展
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摘要
基因型依赖及白苗再生等问题限制了花药离体培养技术潜力的充分发挥。为了克服这些问题,提高花药离体培养效率,国内外学者采用传统遗传学、细胞学、生理生化、分子标记、基因克隆、转录组学及蛋白组学等方法,在小麦、大麦及小黑麦花药预处理、胚状体和愈伤诱导、绿苗再生及白苗形成遗传控制方面进行了大量的研究。本文对这些研究进行综述,以期为相关研究提供参考。
The in vitro anther culture technology is an effective breeding method with the several limitations,such as genotype dependence and regeneration of albino plantlets.In the recent years,in order to overcome these limitations and improve the efficiency of anther culture,many domestic and foreign scholars have focused on studying genetic control on anther pretreatment,embryoid and callus induction,and the regeneration of green and albino plantlets during anther culture for triticeae crops breeding using traditional genetic methods,cytology,physiological and biochemical,molecular markers,gene cloning,transcriptome and proteomics methods.The present review has integrated current research progress to illuminate the genetic mechanisms of anther culture,which may provide references for the related researches.
The in vitro anther culture technology is an effective breeding method with the several limitations,such as genotype dependence and regeneration of albino plantlets.In the recent years,in order to overcome these limitations and improve the efficiency of anther culture,many domestic and foreign scholars have focused on studying genetic control on anther pretreatment,embryoid and callus induction,and the regeneration of green and albino plantlets during anther culture for triticeae crops breeding using traditional genetic methods,cytology,physiological and biochemical,molecular markers,gene cloning,transcriptome and proteomics methods.The present review has integrated current research progress to illuminate the genetic mechanisms of anther culture,which may provide references for the related researches.
引文
[1]IWONA Z,KRZEWSKA M,DUBAS E,et al.Molecular mapping of loci associated with abscisic acid accumulation in triticale(×Triticosecale Wittm.)anthers in response to low temperature stress inducing androgenic development[J].Plant Growth Regulation,2012,68(3):483.
[2]叶兴国,佘茂云,王轲,等.植物组织培养再生相关基因鉴定、克隆和应用研究进展[J].作物学报,2012,38(2):191.YE X G,SHE M Y,WANG K,et al.Identification,cloning,and potential application of genes related to somatic embryogenesis in plant tissue culture[J].Acta Agronomica Sinica,2012,38(2):191.
[3]LANTOS C,WEYEN J,ORSINI J M,et al.Efficient application of in vitro anther culture for different European winter wheat(Triticum aestivum L.)breeding programs[J].Plant Breeding,2013,132(2):149.
[4]JACQUARD C,NOLIN F,HCART C,et al.Microspore embryogenesis and programmed cell death in barley:effects of copper on albinism in recalcitrant cultivars[J].Plant Cell Reports,2009,28(9):1330.
[5]GERMANA M A.Anther culture for haploid and doubled haploid production[J].Plant Cell Tissue and Organ Culture,2011,104(3):286,288.
[6]IWONA Z,DUBAS E,KRZEWSKA M,et al.Antioxidant activity and ROS tolerance in triticale(×Triticosecale Wittm.)anthers affect the efficiency of microspore embryogenesis[J].Plant Cell Tissue and Organ Culture,2014,119(1):79.
[7]KRZEWSKA M,GOEBIOWSKA-PIKANIA G,DUBAS E,et al.Identification of proteins related to microspore embryogenesis responsiveness in anther cultures of winter triticale(×Triticosecale Wittm.)[J].Euphytica,2017,213(8):192.
[8]LABBANI Z,DE BUYSER J,AND PICARD E.Effect of mannitol pre-treatment to improve green plant regeneration on isolated microspore culture in Triticum turgidum ssp.durum cv.‘Jennah Khetifa’[J].Plant Breeding,2007,126(6):565.
[9]MARASCHIN S D F,CASPERS M,POTOKINA E,et al.cDNA array analysis of stress-induced gene expression in barley embryogenesis[J].Physiologia Plantarum,2006,127(4):535.
[10]MUNOZ-AMATRIAIN M,SVENSSON J T,CASTILLO A M,et al.Transcriptome analysis of barley anthers:effect of mannitol treatment on microspore embryogenesis[J].Physiologia Plantarum,2006,127(4):551.
[11]DERIU D,CALACE N,BIANCA M,et al.Morphological and physiological responses of barley plants grown on soils characterized by metal toxicity and metal deficiency[J].Annali Di Chimica,2007,97(3-4):153.
[12]CASTILLO A M,SANCHEZ-DIAZ R A,VALLES M P.Effect of ovary induction on bread wheat anther culture:ovary genotype and developmental stage,and candidate gene association[J].Frontiers in Plant Science,2015,6:402.
[13]LARSEN E T,TUVESSON I K D AND ANDERSEN S B.Nuclear genes affecting percentage of green plants in barley(Hordeum vulgate L.)anther culture[J].Theoretical and Applied Genetics,1991,82(4):417.
[14]叶兴国,徐惠君,徐琼芳,等.小麦花药培养力的基因型差异和配合力分析[J].中国农业科学,1997,30(6):49.YE X G,XU H J,XU Q F,et al.Genetic analysis and combining ability evaluation of the anther culture response in common wheat[J].Scientia Agricultura Sinica,1997,30(6):49.
[15]何玉池,葛杰,陶采成,等.大麦花药培养力的遗传模式分析[J].作物学报,2007,33(4):686.HE Y C,GE J,TAO C C,et al.Genetic control analysis of barley(Hordeum vulgare L.)anther culture response[J].Acta Agronomica Sinica,2007,33(4):686.
[16]LANTOS C AND PAUK J.Anther culture as an effective tool in winter wheat(Triticum aestivum L.)breeding[J].Russian Journal of Genetics,2016,52(8):794.
[17]TUVESSON I K,PEDERSEN S,ANDERSEN S B.Nuclear genes affecting albinism in wheat(Triticum aestivum L.)anther culture[J].Theoretical and Applied Genetics,1989,78:879.
[18]EKIZ H,KONZAK C F.Preliminary diallel analysis of anther culture response in wheat(Triticum aestivum L.)[J].Plant Breeding,1994,113(1):47.
[19]YILDIRIM M,BAHAR B,GENC I,et al.Reciprocal effects in anther cultures of wheat hybrids[J].BIologia Plantarum,2008,52(4):779.
[20]CHAUDHARY H K,DHALIWAL I,SINGH S,et al.Genetics of androgenesis in winter and spring wheat genotypes[J].Euphytica,2003,132(3):311.
[21]SZAKFICS E,KOVACS G,PAUK J,et al.Substitution analysis of callus induction and plant regeneration from anther culture in wheat(Triticum aestivum L.)[J].Plant Cell Reports,1988,7(2):127.
[22]AGACHE S,BACHELIER B,BUYSER J D,et al.Genetic analysis of anther culture response in wheat using a neuploid,chromosome substitution and translocation lines[J].Theoretical and Applied Genetics,1989,77(1):7.
[23]NIELSEN N H,ANDERSEN S U,STOUGAARD J,et al.Chromosomal regions associated with the in vitro culture response of wheat(Triticum aestivum L.)microspores[J].Plant Breeding,2015,134(3):255.
[24]MANNINEN O M.Associations between anther-culture response and molecular markers on chromosomes 2H,3Hand4Hof barley(Hordeum vulgare L.)[J].Theoretical and Applied Genetics,2000,100(1):57.
[25]CHEN X W,CISTUEL,MUNOZ-AMATRIAIN M,et al.Genetic markers for doubled haploid response in barley[J].Euphytica,2007,158(3):287.
[26]VRINTEN P L,NAKAMURA T,KASHA K J.Characterization of cDNAs expressed in the early stages of microspore embryogenesis in barley(Hordeum vulgare L.)[J].Plant Molecular Biology,1999,41(4):455.
[27]GONZALEZ J M,MUNIZ L M,JOUVE N.Mapping of QTLs for androgenetic response based on a molecular genetic map of×Triticosecale Wittmack[J].Genome,2005,48(6):999.
[28]KRZEWSKA M,CZYCZYO-MYSZA I,DUBAS E,et al.Quantitative trait loci associated with androgenic responsiveness in triticale(×Triticosecale Wittm.)anther culture[J].Plant Cell Reports,2012,31(11):2099.
[29]IWONAZUR,DUBAS E,KRZEWSKA M,et al.Hormonal requirements for effective induction of microspore embryogenesis in triticale(×Triticosecale Wittm.)anther cultures[J].Plant Cell Reports,2015,34(1):59.
[30]LAZARIDOU T,PANKOU C,XYNIAS I,et al.Effect of D genome on wheat anther culture response after cold and mannitol pretreatment[J].Acta Biologica Cracoviensia Series Botanica,2016,58(1):95.
[31]TORP A M,HANSEN A L,ANDERSEN S B.Chromosomal regions associated with green plant regeneration in wheat(Triticum aestivum L.)anther culture[J].Euphytica,2001,119(3):377.
[32]MUNOZ-AMATRIAIN M,CASTILLO A M,CHEN X W,et al.Identification and validation of QTLs for green plant percentage in barley(Hordeum vulgare L.)anther culture[J].Molecular Breeding,2008,22(1):119.
[33]ANKELE E,HEBERLE-BORS E,PFOSSER M F,et al.Searching for mechanisms leading to albino plant formation in cereals[J].Physiologiae Plantarum,2005,27(4B):651.
[34]ZHAO P,WANG K,ZHANG W,et al.Comprehensive analysis of differently expressed genes and proteins in albino and green plantlets from a wheat anther culture[J].Biologia Plantarum,2017,61(2):255.
[35]DUNFORD R,WALDEN R M.Plastid genome structure and plastid-related transcript levels in albino barley plants derived from anther culture[J].Current Genetics,1991,20(4):339.
[36]CAREDDA S,DEVAUX P,SANGWAN R S,et al.Plastid ultrastructure and DNA related to albinism in androgenetic embryos of various barley(Hordeum vulgare)cultivars[J].Plant Cell Tissue and Organ Culture,2004,76(1):42.
[37]KRZEWSKA M,CZYCZYO-MYSZA I,DUBAS E,et al.Identification of QTLs associated with albino plant formation and some new facts concerning green versus albino ratio determinants in triticale(×Triticosecale Wittm.)anther culture[J].Euphytica,2015,206(1):263.
[2]叶兴国,佘茂云,王轲,等.植物组织培养再生相关基因鉴定、克隆和应用研究进展[J].作物学报,2012,38(2):191.YE X G,SHE M Y,WANG K,et al.Identification,cloning,and potential application of genes related to somatic embryogenesis in plant tissue culture[J].Acta Agronomica Sinica,2012,38(2):191.
[3]LANTOS C,WEYEN J,ORSINI J M,et al.Efficient application of in vitro anther culture for different European winter wheat(Triticum aestivum L.)breeding programs[J].Plant Breeding,2013,132(2):149.
[4]JACQUARD C,NOLIN F,HCART C,et al.Microspore embryogenesis and programmed cell death in barley:effects of copper on albinism in recalcitrant cultivars[J].Plant Cell Reports,2009,28(9):1330.
[5]GERMANA M A.Anther culture for haploid and doubled haploid production[J].Plant Cell Tissue and Organ Culture,2011,104(3):286,288.
[6]IWONA Z,DUBAS E,KRZEWSKA M,et al.Antioxidant activity and ROS tolerance in triticale(×Triticosecale Wittm.)anthers affect the efficiency of microspore embryogenesis[J].Plant Cell Tissue and Organ Culture,2014,119(1):79.
[7]KRZEWSKA M,GOEBIOWSKA-PIKANIA G,DUBAS E,et al.Identification of proteins related to microspore embryogenesis responsiveness in anther cultures of winter triticale(×Triticosecale Wittm.)[J].Euphytica,2017,213(8):192.
[8]LABBANI Z,DE BUYSER J,AND PICARD E.Effect of mannitol pre-treatment to improve green plant regeneration on isolated microspore culture in Triticum turgidum ssp.durum cv.‘Jennah Khetifa’[J].Plant Breeding,2007,126(6):565.
[9]MARASCHIN S D F,CASPERS M,POTOKINA E,et al.cDNA array analysis of stress-induced gene expression in barley embryogenesis[J].Physiologia Plantarum,2006,127(4):535.
[10]MUNOZ-AMATRIAIN M,SVENSSON J T,CASTILLO A M,et al.Transcriptome analysis of barley anthers:effect of mannitol treatment on microspore embryogenesis[J].Physiologia Plantarum,2006,127(4):551.
[11]DERIU D,CALACE N,BIANCA M,et al.Morphological and physiological responses of barley plants grown on soils characterized by metal toxicity and metal deficiency[J].Annali Di Chimica,2007,97(3-4):153.
[12]CASTILLO A M,SANCHEZ-DIAZ R A,VALLES M P.Effect of ovary induction on bread wheat anther culture:ovary genotype and developmental stage,and candidate gene association[J].Frontiers in Plant Science,2015,6:402.
[13]LARSEN E T,TUVESSON I K D AND ANDERSEN S B.Nuclear genes affecting percentage of green plants in barley(Hordeum vulgate L.)anther culture[J].Theoretical and Applied Genetics,1991,82(4):417.
[14]叶兴国,徐惠君,徐琼芳,等.小麦花药培养力的基因型差异和配合力分析[J].中国农业科学,1997,30(6):49.YE X G,XU H J,XU Q F,et al.Genetic analysis and combining ability evaluation of the anther culture response in common wheat[J].Scientia Agricultura Sinica,1997,30(6):49.
[15]何玉池,葛杰,陶采成,等.大麦花药培养力的遗传模式分析[J].作物学报,2007,33(4):686.HE Y C,GE J,TAO C C,et al.Genetic control analysis of barley(Hordeum vulgare L.)anther culture response[J].Acta Agronomica Sinica,2007,33(4):686.
[16]LANTOS C AND PAUK J.Anther culture as an effective tool in winter wheat(Triticum aestivum L.)breeding[J].Russian Journal of Genetics,2016,52(8):794.
[17]TUVESSON I K,PEDERSEN S,ANDERSEN S B.Nuclear genes affecting albinism in wheat(Triticum aestivum L.)anther culture[J].Theoretical and Applied Genetics,1989,78:879.
[18]EKIZ H,KONZAK C F.Preliminary diallel analysis of anther culture response in wheat(Triticum aestivum L.)[J].Plant Breeding,1994,113(1):47.
[19]YILDIRIM M,BAHAR B,GENC I,et al.Reciprocal effects in anther cultures of wheat hybrids[J].BIologia Plantarum,2008,52(4):779.
[20]CHAUDHARY H K,DHALIWAL I,SINGH S,et al.Genetics of androgenesis in winter and spring wheat genotypes[J].Euphytica,2003,132(3):311.
[21]SZAKFICS E,KOVACS G,PAUK J,et al.Substitution analysis of callus induction and plant regeneration from anther culture in wheat(Triticum aestivum L.)[J].Plant Cell Reports,1988,7(2):127.
[22]AGACHE S,BACHELIER B,BUYSER J D,et al.Genetic analysis of anther culture response in wheat using a neuploid,chromosome substitution and translocation lines[J].Theoretical and Applied Genetics,1989,77(1):7.
[23]NIELSEN N H,ANDERSEN S U,STOUGAARD J,et al.Chromosomal regions associated with the in vitro culture response of wheat(Triticum aestivum L.)microspores[J].Plant Breeding,2015,134(3):255.
[24]MANNINEN O M.Associations between anther-culture response and molecular markers on chromosomes 2H,3Hand4Hof barley(Hordeum vulgare L.)[J].Theoretical and Applied Genetics,2000,100(1):57.
[25]CHEN X W,CISTUEL,MUNOZ-AMATRIAIN M,et al.Genetic markers for doubled haploid response in barley[J].Euphytica,2007,158(3):287.
[26]VRINTEN P L,NAKAMURA T,KASHA K J.Characterization of cDNAs expressed in the early stages of microspore embryogenesis in barley(Hordeum vulgare L.)[J].Plant Molecular Biology,1999,41(4):455.
[27]GONZALEZ J M,MUNIZ L M,JOUVE N.Mapping of QTLs for androgenetic response based on a molecular genetic map of×Triticosecale Wittmack[J].Genome,2005,48(6):999.
[28]KRZEWSKA M,CZYCZYO-MYSZA I,DUBAS E,et al.Quantitative trait loci associated with androgenic responsiveness in triticale(×Triticosecale Wittm.)anther culture[J].Plant Cell Reports,2012,31(11):2099.
[29]IWONAZUR,DUBAS E,KRZEWSKA M,et al.Hormonal requirements for effective induction of microspore embryogenesis in triticale(×Triticosecale Wittm.)anther cultures[J].Plant Cell Reports,2015,34(1):59.
[30]LAZARIDOU T,PANKOU C,XYNIAS I,et al.Effect of D genome on wheat anther culture response after cold and mannitol pretreatment[J].Acta Biologica Cracoviensia Series Botanica,2016,58(1):95.
[31]TORP A M,HANSEN A L,ANDERSEN S B.Chromosomal regions associated with green plant regeneration in wheat(Triticum aestivum L.)anther culture[J].Euphytica,2001,119(3):377.
[32]MUNOZ-AMATRIAIN M,CASTILLO A M,CHEN X W,et al.Identification and validation of QTLs for green plant percentage in barley(Hordeum vulgare L.)anther culture[J].Molecular Breeding,2008,22(1):119.
[33]ANKELE E,HEBERLE-BORS E,PFOSSER M F,et al.Searching for mechanisms leading to albino plant formation in cereals[J].Physiologiae Plantarum,2005,27(4B):651.
[34]ZHAO P,WANG K,ZHANG W,et al.Comprehensive analysis of differently expressed genes and proteins in albino and green plantlets from a wheat anther culture[J].Biologia Plantarum,2017,61(2):255.
[35]DUNFORD R,WALDEN R M.Plastid genome structure and plastid-related transcript levels in albino barley plants derived from anther culture[J].Current Genetics,1991,20(4):339.
[36]CAREDDA S,DEVAUX P,SANGWAN R S,et al.Plastid ultrastructure and DNA related to albinism in androgenetic embryos of various barley(Hordeum vulgare)cultivars[J].Plant Cell Tissue and Organ Culture,2004,76(1):42.
[37]KRZEWSKA M,CZYCZYO-MYSZA I,DUBAS E,et al.Identification of QTLs associated with albino plant formation and some new facts concerning green versus albino ratio determinants in triticale(×Triticosecale Wittm.)anther culture[J].Euphytica,2015,206(1):263.