小麦食源性过敏原致敏亚基分析
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摘要
通过SDS-PAGE对小麦亚基成分分析,采用ImmunoCAP 250全自动体外免疫检测系统测定小麦过敏患者不同个体对小麦致敏蛋白的识别结果,并以我国12例小麦过敏患者的血清为探针,3例正常人的血清池为对照,与师栾02-1小麦粉进行还原态一维电泳免疫印迹分析,鉴定引起我国小麦过敏人群过敏的小麦过敏原及其致敏概率。结果表明,小麦过敏原蛋白亚基的致敏概率自高至低的顺序依次为17 ku(6/12)、28ku(6/12)、14ku(5/12)、19ku(5/12)、22ku(5/12)、24ku(5/12)、25 ku(5/12)、26ku(5/12)、11 ku(4/12)、12ku(4/12)、16 ku(4/12)、21 ku(4/12)、27 ku(4/12)、18 ku(3/12)、30 ku(3/12)、31 ku(3/12)、36ku(3/12)、47ku(3/12)、83ku(3/12),其他一些蛋白亚基致敏概率为1/6或1/12。
In this paper, the immune CAP-250 automatic in vitro immunoassay system was used to determine the identification results of allergenic wheat protein in wheat allergy patients through the analysis of the subunits of wheat by SDS-PAGE. And the sera of 12 wheat allergy patients in China were used as the probes and the serum pool of 3 negative persons was used as the control. And the wheat allergens and allergenicity for allergic people of China were identified by the one-dimensional electrophoretic western blotting analysis of Shiluan No.02-1 wheat flour. The results showed that the allergenic probabilities of wheat allergenic protein subunits were 17 kD(6/12),28 ku(6/12), 14 ku(5/12), 19 ku(5/12), 22 ku(5/12),24 ku(5/12), 25 ku(5/12),26 ku(5/12), 11 ku(4/12), 12 ku(4/12), 16 ku(4/12), 21 ku(4/12), 27 ku(4/12), 18 ku(3/12), 30 ku(3/12), 31 ku(3/12), 36 ku(3/12),47 ku(3/12),83 ku(3/12), the probability of some other allergenic protein subunits was 1/6 or1/12.
In this paper, the immune CAP-250 automatic in vitro immunoassay system was used to determine the identification results of allergenic wheat protein in wheat allergy patients through the analysis of the subunits of wheat by SDS-PAGE. And the sera of 12 wheat allergy patients in China were used as the probes and the serum pool of 3 negative persons was used as the control. And the wheat allergens and allergenicity for allergic people of China were identified by the one-dimensional electrophoretic western blotting analysis of Shiluan No.02-1 wheat flour. The results showed that the allergenic probabilities of wheat allergenic protein subunits were 17 kD(6/12),28 ku(6/12), 14 ku(5/12), 19 ku(5/12), 22 ku(5/12),24 ku(5/12), 25 ku(5/12),26 ku(5/12), 11 ku(4/12), 12 ku(4/12), 16 ku(4/12), 21 ku(4/12), 27 ku(4/12), 18 ku(3/12), 30 ku(3/12), 31 ku(3/12), 36 ku(3/12),47 ku(3/12),83 ku(3/12), the probability of some other allergenic protein subunits was 1/6 or1/12.
引文
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[5]BITTNER C, PETERS U, FRENZEL K, et al. New wheat allergens related to baker's asthma[J]. Journal of Allergy and Clinical Immunology, 2015, 136(5):1416-1418
[6]SANDER I, FLAGGE A, MERGET R, et al. Identification of wheat floor allergens by means of 2-dimensional immunoblotting[J]. Journal of Allergy and Clinical Immunology,2001, 107(5):907-913
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[12]贾祥祥,张强涛,王圣宝,等.2015年小麦主产区部分强筋小麦品质现状及分析[J].粮食与饲料工业,2016(10):4-10JIA X X, ZHANG Q T, WANG S B, et al. Quality status and analysis of some strong gluten wheat in wheat production area in 2015[J].Cereal&Feed Industry,2016(10):4-10
[13]AKAGAWA M, HANDOYO T, ISHII T, et al. Proteomic analysis of wheat flour allergens[J]. J Agric Food Chem,2007, 55(17):6863-6870
[14]尹佳,文利平.小麦依赖运动诱发的严重过敏反应——15例临床病例观察[A].中华医学会变态反应学分会.中华医学会2009年全国变态反应学术会议论文汇编[C].中华医学会变态反应学分会,2009:5YIN J, WEN L P. Wheat dependant exercise-induced anaphylaxis-15 clinical cases were observed[A]. Chinese society of allergy. Chinese medical association 2009 national allergy academic conference paper compilation[C]. Chinese Society of Allergy,2009:5
[15]LEE S, LEE S, JO E, et al. Wheat-induced anaphylaxis in korean adults:a report of 6 cases[J]. Clinical Nutrition Research, 2013,2(1):76-79
[16]李玺,薛文通.小麦致敏蛋白及其过敏病症研究进展[J].食品科技,2016(8):147-152LI X, XUE W T. Research progress of wheat allergens and allergy symptoms[J]. Food Science and Technology, 2016(8):147-152
[17]李慧静.超高静压协同酶法降低专用大豆分离蛋白致敏性的研究[D].无锡:江南大学,2013LI H J. Study about allergenicity reducing of special soy protein isolate by high hydrostatic pressure assisting enzymatic approach[D]. Wuxi:Jiangnan University,2013
[18]SOTKOVSKY P, SKLENAR J, HALADA P, et al. A new approach to the isolation and characterization of wheat flour allergens[J]. Wuxi:Clinical and Experimental Allergy,2011,41(7):1031-1043.
[2]BATTAIS F, APARICIO C,KANNY G, et al. Allergenicity of wheat flour[J]. Allergic et immunologie, 2002, 34(3):98-100
[3]WHO/IUIS allergen nomenclature home page[DB/OL]. http://www.allergen.org/index.php
[4]SANDER I, RIHS H, DOEKES G, et al. Component-re-solved diagnosis of baker's allergy based on specific IgE to recombinant wheat flour proteins[J]. Journal of Allergy and Clinical Immunology, 2015, 135(6):1529-1537
[5]BITTNER C, PETERS U, FRENZEL K, et al. New wheat allergens related to baker's asthma[J]. Journal of Allergy and Clinical Immunology, 2015, 136(5):1416-1418
[6]SANDER I, FLAGGE A, MERGET R, et al. Identification of wheat floor allergens by means of 2-dimensional immunoblotting[J]. Journal of Allergy and Clinical Immunology,2001, 107(5):907-913
[7]WEISS W, HUBER G,ENGELnge K H, et al. Identification and characterization of wheat grain albumin/globulin allergens[J]. Electrophoresis, 1997, 18(5):826-833
[8]VENTER C, MASLIN K, ARSHAD S H, et al. Very low prevalence of IgE mediated wheat allergy and high levels of cross-sensitisation between grass and wheat in a UK birth cohort[J]. Clinical and Translational Allergy, 2016, 6:22
[9]NILSSON N, SJOLANDER S, BAAR A, et al. Wheat allergy in children evaluated with challenge and IgE antibodies to wheat components[J]. Pediatric Allergy and Immunology,2015, 26(2):119-125
[10]朱奕锜,王朵勤,胡跃,等.小麦依赖-运动诱发的严重过敏反应:对10例病例的临床研究及小麦蛋白过敏原分析[A].中国中西医结合学会皮肤性病专业委员会.2017全国中西医结合皮肤性病学术年会论文汇编[C].中国中西医结合学会皮肤性病专业委员会,2017:1ZHU Y Q, WANG D Q, HU Y, et al. Wheat dependant exercise-induced anaphylaxis:a clinical study of 10 cases and analysis of wheat protein allergens[A]. China Society of Integrated Traditional Chinese and Western Medicine Skin Venereal Disease Professional Committee. 2017 national academic annual conference on skin venereal diseases in combination with traditional Chinese and western medicine[C].China Society of Integrated Traditional Chinese and Western Medicine Skin Venereal Disease Professional Committee,2017:1
[11]冼静雯,吴海强,简晓莉,等.小麦和荞麦过敏原的分离与免疫学特性初步鉴定[J].麦类作物学报,2008(5):799-803XIAN J W, WU H Q, JIAN X L, et al. Extraction, isolation and immunological identification of allergens from triticum aestivum and fagopyrum esculentum[J]. Journal of Triticeae Crops, 2008(5):799-803
[12]贾祥祥,张强涛,王圣宝,等.2015年小麦主产区部分强筋小麦品质现状及分析[J].粮食与饲料工业,2016(10):4-10JIA X X, ZHANG Q T, WANG S B, et al. Quality status and analysis of some strong gluten wheat in wheat production area in 2015[J].Cereal&Feed Industry,2016(10):4-10
[13]AKAGAWA M, HANDOYO T, ISHII T, et al. Proteomic analysis of wheat flour allergens[J]. J Agric Food Chem,2007, 55(17):6863-6870
[14]尹佳,文利平.小麦依赖运动诱发的严重过敏反应——15例临床病例观察[A].中华医学会变态反应学分会.中华医学会2009年全国变态反应学术会议论文汇编[C].中华医学会变态反应学分会,2009:5YIN J, WEN L P. Wheat dependant exercise-induced anaphylaxis-15 clinical cases were observed[A]. Chinese society of allergy. Chinese medical association 2009 national allergy academic conference paper compilation[C]. Chinese Society of Allergy,2009:5
[15]LEE S, LEE S, JO E, et al. Wheat-induced anaphylaxis in korean adults:a report of 6 cases[J]. Clinical Nutrition Research, 2013,2(1):76-79
[16]李玺,薛文通.小麦致敏蛋白及其过敏病症研究进展[J].食品科技,2016(8):147-152LI X, XUE W T. Research progress of wheat allergens and allergy symptoms[J]. Food Science and Technology, 2016(8):147-152
[17]李慧静.超高静压协同酶法降低专用大豆分离蛋白致敏性的研究[D].无锡:江南大学,2013LI H J. Study about allergenicity reducing of special soy protein isolate by high hydrostatic pressure assisting enzymatic approach[D]. Wuxi:Jiangnan University,2013
[18]SOTKOVSKY P, SKLENAR J, HALADA P, et al. A new approach to the isolation and characterization of wheat flour allergens[J]. Wuxi:Clinical and Experimental Allergy,2011,41(7):1031-1043.