基于共振光散射光谱法测定药品中盐酸肼屈嗪含量
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摘要
为了研究药品中盐酸肼屈嗪含量检测的新方法,本文采用共振光散射光谱法以Clark-Lubs缓冲液为介质分析盐酸肼屈嗪与十二烷基苯磺酸钠反应的光散射强度参数关系。研究发现:在pH 1.4的Clark-Lubs缓冲介质中适量盐酸肼屈嗪与十二烷基苯磺酸钠水溶液发生π-π电荷转移和离子缔合反应,所得产物的共振光散射最大发射波长在402 nm处,盐酸肼屈嗪浓度在0.125～1.00 mg·L~(-1)范围内呈线性关系,相关系数R为0.9998,检出限为0.035 mg·L~(-1),回收率在99.08%～100.8%之间。所拟方法适用于分析常温下盐酸肼屈嗪与光散射强度的参数关系,可应用于药品中盐酸肼屈嗪含量的测定。
In order to study the new method for the determination of hydralazine hydrochloride in pharmaceuticals,the light scattering intensity parameters of the reaction between hydralazine hydrochloride and sodium dodecyl benzene sulfonate were studied using the resonance light scattering spectrometry in Clark-Lubs buffer medium.The results showed that the reaction between the proper amount of hydrazinium hydrochloride and sodium dodecyl benzene sulfonate aqueous solution in the pH 1.4 Clark-Lubs buffer medium was π-π charge transfer and ion association reaction mechanism.The maximum emission wavelength of the resonance light scattering of the obtained product at 402 nm.The hydralazine hydrochloride concentration was linear in the range of 0.125 to 1.00 mg·L~(-1).The correlation coefficient R was 0.9998.The detection limit was 0.035 mg·L~(-1),and the recovery rate was 99.08～100.8%.The proposed method is suitable for analyzing the parametric relationship between hydralazine hydrochloride and light scattering intensity at room temperature,and can be applied to the determination of hydralazine hydrochloride in medicines.
In order to study the new method for the determination of hydralazine hydrochloride in pharmaceuticals,the light scattering intensity parameters of the reaction between hydralazine hydrochloride and sodium dodecyl benzene sulfonate were studied using the resonance light scattering spectrometry in Clark-Lubs buffer medium.The results showed that the reaction between the proper amount of hydrazinium hydrochloride and sodium dodecyl benzene sulfonate aqueous solution in the pH 1.4 Clark-Lubs buffer medium was π-π charge transfer and ion association reaction mechanism.The maximum emission wavelength of the resonance light scattering of the obtained product at 402 nm.The hydralazine hydrochloride concentration was linear in the range of 0.125 to 1.00 mg·L~(-1).The correlation coefficient R was 0.9998.The detection limit was 0.035 mg·L~(-1),and the recovery rate was 99.08～100.8%.The proposed method is suitable for analyzing the parametric relationship between hydralazine hydrochloride and light scattering intensity at room temperature,and can be applied to the determination of hydralazine hydrochloride in medicines.
引文
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[11] 周斌,石启炜,黄凌燕,等.纳米金共振光散射光谱法和比色法检测牛奶中庆大霉素[J].分析实验室,2017,36(11):1316-1319.(ZHOU Bin,SHI Qiwei,HUANG Lingyan,et al.Determination of gentamicin in milk based on gold nanoparticles by resonance light scattering and colorimetry[J].Chinese Journal of Analytical Laboratory,2017,36(11):1316-1319.)
[12] 吴媛媛,郭立芸,朱弘元,等.共振光散射法测定啤酒中蛋白酶A活力[J].中外酒业,2016,1:32-37.(WU Yuanyuan,GUO Liyun.ZHU Hongyuan,et al.Determination of protease a activity in beer by resonance light scattering spectrometry[J]Chinese and foreign wine industry,2016,1:32-37.)
[13] 邹明静,王淑玉,王英玲.纳米金标记共振光散射法均相测定神经元特异性烯醇化酶[J].分析实验室,2017,36(10):1198-1201.(ZOU Mingjing,WANG Shuyun,WANG Yinling.Homogeneous resonance light scattering spectral assay of neuron specific enolase based on gold labeling[J].Chinese Journal of Analytical Laboratory,2017,36(10):1198-1201.)
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[15] 郭宗宁,曾绍汉,戚琦.日落黄-十六烷基三甲基溴化铵共振光散射法测定核酸[J].化学研究与应用,2017,29(1):44-48.(GUO Zongling,ZENG Shaohan,QI Qi.Determination of nucleic acids by reonance light scattering of sunset yellow with CTMAB[J].Chemical Research and Application,2017,29(1):44-48.)
[16] 何宝光,杨红露.共振光散射法测定稀土元素镱(Ⅲ)[J].四川环境,2017,36(2):26-29.(HE Baoguang,YANG Honglu.Determination of ytterbium (Ⅲ) by resonance light-scattering spectrum[J].Sichuan Environment,2017,36(2):26-29.)
[17] 周斌,胡淦彬,杨慧仙,等.基于DNAzyme与铀特异性反应的共振光散射法检测铀[J].分析实验室,2016,35(1):12-16.(ZHOU Bin,HU Ganbin,YANG Huixian,et al.Resonance light scattering method for the determination of uranium based on the specific reaction of DNAzyme and uranium[J].Chinese Journal of Analytical Laboratory,2016,35(1):12-16.)
[18] 周尚,杨季冬,吴佳辉,等.格拉司琼与磷钨杂多酸相互作用的共振光散射及分析应用[J].光散射学报,2012,24(4):389-391.(ZHOU Shang,YANG Jidong,WU Jiahui,et al.Resonance light scattering of the interaction of granisetron with phosphotungstic acid and its analytical application[J].The Journal of Light Scattering,2012,24(4):389-391.)
[2] KANDLER M R,MAH G T,TEJANIA M,et al.Hydralazine for Essential Hypertension[J].Cochrane Lib,2010,8:1-16.
[3] 国家药典委员会.中华人民共和国药典2015年二部[M].中国医药科技出版社,2015,1025.(National Pharmacopoeia Committee.Pharmacopoeia of the People's Republic of China 2015 Edition II[M].China Medical Science and Technology Press,2015,1025.)
[4] LIU Y Y,LI H,LUO H J,et al.LC-MS-MS Method for quantification of hydralazine in balb/c mouse plasma and brain:application to pharm acokinetic study[J].Chromatographia,2011,73 (11):1183 -1188.
[5] 邱颖恒,周敏贤,李玉兰.反相高效液相色谱法测定盐酸肼屈嗪片的含量[J].中国药师,2008,11(11):1305-1307.(QIU Yingheng,ZHOU Minxian,LI Yulan.Determination of chlorindazine hydrochloride tablets by RP-HPLC[J].Chinese Pharmacist,2008,11(11):1305-1307.)
[6] 陈建福,陈健旋,施伟梅.鲁米诺-铁氰化钾化学发光法测定盐酸肼屈嗪[J].沈阳大学学报(自然科学版),2012,(6):30-33.(CHEN Jianfu,CHEN Jianxuan,SHI Weimei.Determination of hydralazine hydrochloride by chemiluminescence of luminol-potassium ferricyanide[J].Journal of Shenyang University (Natural Science Edition),2012,(6):30-33.)
[7] XIONG YAN,ZHOU HOUJIANG,ZHANG ZHUJUN,et al.Determination of hydralazine with flow injection chemilumi-nescence sensor using molecularly imprinted polymer as recognition element[J].J Pharmaceut Biomed,2006,41:594-700.
[8] SHOUKRY A F,ISSA Y M,IBRAHIM H,et al.Atomic emission spectrometric determination of antazoline,hydralazine and amiloride hydrochlorides,and quinine sulfate based on formation of ion associates with manganese thiocyanate[J].Analyst,1995,120(4):1211-1213.
[9] DANIELSON N D,BARTOLO R G.Determination of Hydralazine in Tablets by FluorescenceE[J].Analytical Letters,1983,16(5):343-354.
[10] 付海波,詹蕾,王健.硼酸催化茚三酮与盐酸肼屈嗪的反应及分析应用[J].西南师范大学学报:自然科学版,2012,37(5):43-48.(FU Haibo,ZHAN Lei,WANG Jian.Boric acid catalyzes the reaction and analysis of ninhydrin and hydralazine hydrochloride[J].Journal of Southwest China Normal University:Natural Science Edition,2012,37(5):43-48.)
[11] 周斌,石启炜,黄凌燕,等.纳米金共振光散射光谱法和比色法检测牛奶中庆大霉素[J].分析实验室,2017,36(11):1316-1319.(ZHOU Bin,SHI Qiwei,HUANG Lingyan,et al.Determination of gentamicin in milk based on gold nanoparticles by resonance light scattering and colorimetry[J].Chinese Journal of Analytical Laboratory,2017,36(11):1316-1319.)
[12] 吴媛媛,郭立芸,朱弘元,等.共振光散射法测定啤酒中蛋白酶A活力[J].中外酒业,2016,1:32-37.(WU Yuanyuan,GUO Liyun.ZHU Hongyuan,et al.Determination of protease a activity in beer by resonance light scattering spectrometry[J]Chinese and foreign wine industry,2016,1:32-37.)
[13] 邹明静,王淑玉,王英玲.纳米金标记共振光散射法均相测定神经元特异性烯醇化酶[J].分析实验室,2017,36(10):1198-1201.(ZOU Mingjing,WANG Shuyun,WANG Yinling.Homogeneous resonance light scattering spectral assay of neuron specific enolase based on gold labeling[J].Chinese Journal of Analytical Laboratory,2017,36(10):1198-1201.)
[14] 张传林,胡庆红.基质固相分散-共振光散射法测定蔬果中的甲基毒死蜱[J].河南农业科学,2016,45(8):81-85.(ZHANG Chuanlin,HU Qinghong.Determination of chlorpyrifos-methyl in vegetables and fruit by matrix solid-phase dispersion extraction and resonance light scatterring method[J].Journal of Henan Agricultural Sciences,2016,45(8):81-85.)
[15] 郭宗宁,曾绍汉,戚琦.日落黄-十六烷基三甲基溴化铵共振光散射法测定核酸[J].化学研究与应用,2017,29(1):44-48.(GUO Zongling,ZENG Shaohan,QI Qi.Determination of nucleic acids by reonance light scattering of sunset yellow with CTMAB[J].Chemical Research and Application,2017,29(1):44-48.)
[16] 何宝光,杨红露.共振光散射法测定稀土元素镱(Ⅲ)[J].四川环境,2017,36(2):26-29.(HE Baoguang,YANG Honglu.Determination of ytterbium (Ⅲ) by resonance light-scattering spectrum[J].Sichuan Environment,2017,36(2):26-29.)
[17] 周斌,胡淦彬,杨慧仙,等.基于DNAzyme与铀特异性反应的共振光散射法检测铀[J].分析实验室,2016,35(1):12-16.(ZHOU Bin,HU Ganbin,YANG Huixian,et al.Resonance light scattering method for the determination of uranium based on the specific reaction of DNAzyme and uranium[J].Chinese Journal of Analytical Laboratory,2016,35(1):12-16.)
[18] 周尚,杨季冬,吴佳辉,等.格拉司琼与磷钨杂多酸相互作用的共振光散射及分析应用[J].光散射学报,2012,24(4):389-391.(ZHOU Shang,YANG Jidong,WU Jiahui,et al.Resonance light scattering of the interaction of granisetron with phosphotungstic acid and its analytical application[J].The Journal of Light Scattering,2012,24(4):389-391.)