水稻叶色突变体ygr的遗传分析与基因定位
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摘要
叶色突变体是研究水稻叶绿体发育、光合色素合成与降解的理想材料。前期研究在水稻保持系大面积繁殖时发现一株转绿型淡黄叶突变体ygr,为了阐明其叶色调控机理,本试验比较了ygr与野生型的主要农艺性状和光合色素含量,并对该突变体进行了遗传分析和基因定位。结果表明,与野生型相比,ygr的始穗期推迟2 d,株高、单株有效穗数、穗长、穗粒数、结实率和千粒重等主要农艺性状均无显著差异,而苗期和抽穗期的叶绿素a、叶绿素b、总叶绿素和类胡萝卜素等光合色素含量均显著降低。遗传分析表明,ygr叶色突变性状受单隐性核基因控制,暂时将其命名为YGR。以ygr与日本晴杂交的F_2群体作为定位群体,将YGR基因定位在水稻第1号染色体分子标记WY8到WY20之间,其遗传距离分别为0.2 cM和0.5 cM。本研究结果为该基因的克隆和功能分析以及解析其突变机制奠定了基础。
Leaf color mutant was an ideal material for studying chloroplast development,photosynthetic pigment synthesis and degradation in rice. In the early study,a green revertible leaf color mutants ygr was obtained from extened propagation of rice maintainers. In order to clarify the leaf color regulation mechanism,the main agronomic traits and photosynthetic pigment content of ygr were compared with wild type,and genetic analysis and gene mapping of this mutant were conducted in this study. The results showed that initial heading stage of ygr was delayed by 2 days compared to the wild type,and the plant height,the no. of effective panicles per plant,the panicle length,the no. of grains per panicle,seed setting rate and 1 000 grain weight were similar with the wild type. Genetic analysis showed that the leaf color mutant traits of ygr were controlled by a single recessive nuclear gene,and was temporarily named YGR.With the F_2 population developed from the cross between ygr and the green leaf variety Nipponbare,the YGR gene was mappled between WY8 and WY20,and the genetic distances were 0. 2 cM and 0. 5 cM,respectively. This study laid the foundation for futher cloning and functional analysis of this gene and to analyse the mutation mechanism.
Leaf color mutant was an ideal material for studying chloroplast development,photosynthetic pigment synthesis and degradation in rice. In the early study,a green revertible leaf color mutants ygr was obtained from extened propagation of rice maintainers. In order to clarify the leaf color regulation mechanism,the main agronomic traits and photosynthetic pigment content of ygr were compared with wild type,and genetic analysis and gene mapping of this mutant were conducted in this study. The results showed that initial heading stage of ygr was delayed by 2 days compared to the wild type,and the plant height,the no. of effective panicles per plant,the panicle length,the no. of grains per panicle,seed setting rate and 1 000 grain weight were similar with the wild type. Genetic analysis showed that the leaf color mutant traits of ygr were controlled by a single recessive nuclear gene,and was temporarily named YGR.With the F_2 population developed from the cross between ygr and the green leaf variety Nipponbare,the YGR gene was mappled between WY8 and WY20,and the genetic distances were 0. 2 cM and 0. 5 cM,respectively. This study laid the foundation for futher cloning and functional analysis of this gene and to analyse the mutation mechanism.
引文
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[13]刘钰龙,刘峰,周坤能,苏晓妹,方先文,张云辉,鲍依群.水稻温敏型叶片白化突变体tsa1的表型鉴定和基因定位[J].作物学报,2016,42(12):1754-1763
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[15]Jung K H,Hur J,Ryu C H,Choi Y,Chung Y Y,Miyao A,Hirochika H,An G.Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system[J].Plant&Cell Physiology,2003,44(5):463-472
[16]Wang P,Gao J,Wan C,Zhang F,Xu Z,Huang X,Sun X,Deng X.Divinyl chlorophyll(ide)a can be converted to monovinyl chlorophyll(ide)a by a divinyl reductase in rice[J].Plant Physiology,2010,153(3):994-1003
[17]Gothandam K M,Kim E S,Cho H,Chung Y Y.Os PPR1,a pentatricopeptide repeat protein of rice is essential for the chloroplast biogenesis[J].Plant Molecular Biology,2005,58(3):421-433
[18]Kusumi K,Yara A,Mitsui N,Tozawa Y,Iba K.Characterization of a rice nuclear-encoded plastid RNA polymerase gene OsRpo Tp[J].Plant&Cell Physiology,2004,45(9):1194-1201
[19]Hu F,Kang Z,Qiu S,Wang Y,Qin F,Yue C,Huang J,Wang G.Overexpression of Os TLP27 in rice improves chloroplast function and photochemical efficiency[J].Plant Science,2012,195(3):125-134
[20]Wang F,Wang G,Li X,Huang J,Zheng J.Heredity,physiology and mapping of a chlorophyll content gene of rice(Oryza sativa L.)[J].Journal of Plant Physiology,2008,165(3):324-330
[21]Huang J,Qin F,Zang G,Kang Z,Zou H,Hu F,Yue C,Li X,Wang G.Mutation of Os DET1 increases chlorophyll content in rice[J].Plant Science,2013,210:241-249
[22]张力科,李志彬,刘海燕,李如海,陈满元,陈爱国,钱益亮,华泽田,高用明,朱苓华.两个新的水稻叶色突变体形态结构与遗传定位研究[J].中国农业科学,2010,43(2):223-229
[23]Kong Z,Xue Y.A novel nuclear-localized CCCH-Type zinc finger protein,Os DOS,is involved in delaying leaf senescence in rice[J].Plant Physiology,2006,141(4):1376-1388
[24]Kusaba M,Ito H,Morita R,Iida S,Sato Y,Fujimoto M,Kawasaki S,Tanaka R,Hirochika H,Nishimura M.Rice non-yellow coloring1 is involved in light-harvesting complex II and grana degradation during leaf senescence[J].Plant Cell,2007,19(4):1362-1375
[25]Sato Y,Morita R,Katsuma S,Nishimura M,Tanaka A,Kusaba M.Two short-chain dehydrogenase/reductases,non-yellow coloring1 and nyc1-like,are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice[J].Plant Journal,2009,57(1):120-131
[26]李红昌,钱前,王斌,李晓波,朱立煌,徐吉臣.水稻白穗突变体基因的鉴定和染色体定位[J].科学通报,2003,48(3):268-270
[27]陈佳颖,赵剑,刘晓,李超,林冬枝,董彦君,叶胜海,张小明.一个新水稻温敏感叶色突变体的遗传分析及其基因分子定位[J].植物学报,2010,45(4):419-425
[28]Dong H,Fei G L,Wu C Y,Wu F Q,Sun Y Y,Chen M J,Ren YL,Zhou K N,Cheng Z J,Wang J L.A rice virescent-yellow leaf mutant reveals new insights into the role and assembly of plastid caseinolytic protease in higher plants[J].Plant Physiology,2013,162(4):1867-1880
[29]程欣,任德勇,马娇,朱晓燕,桑贤春,凌英华,赵芳明,何光华.水稻叶片灰白转黄突变体pyr1的鉴定与基因定位[J].作物学报,2013,39(6):992-998
[30]李广贤,姚方印,侯恒军,孙召文,姜明松,朱文银,周学标.水稻黄绿叶突变体ygl209的遗传分析与目标基因精细定位[J].作物学报,2015,41(10):1603-1611
[31]胡智,何蔷薇,郭旻,李荣德,朱茜,刘敏,严长杰.1个新水稻黄叶突变体yl1的遗传分析和初步定位[J].扬州大学学报(农业与生命科学版),2016,37(2):51-55
[2]魏彦林,施勇烽,吴建利.水稻核基因控制的叶色变异[J].核农学报,2011,25(6):1169-1178
[3]梅淑芳,王勇强,赵华,舒小丽,吴殿星.叶色标记不育系与多分蘖突变体选育优质杂交水稻新组合浙辐两优12[J].核农学报,2013,27(4):425-429
[4]贺治洲,尹明,谢振宇,沈建凯,王悦.水稻转绿型叶色突变体研究进展[J].热带农业科学,2014,34(8):30-36
[5]李燕群,高家旭,肖云华,李秀兰,蒲翔,孙昌辉,王平荣,邓晓建.水稻ygl80黄绿叶突变体的遗传分析与目标基因精细定位[J].作物学报,2014,40(4):644-649
[6]孙小秋,王兵,肖云华,万春美,邓晓建,王平荣.水稻ygl98黄绿叶突变基因的精细定位与遗传分析[J].作物学报,2011,37(6):991-997
[7]张天泉,郭爽,邢亚迪,杜丹,桑贤春,凌英华,何光华.水稻新黄绿叶基因YGL9的分子定位[J].作物学报,2015,41(7):989-997
[8]Mccouch S R,Teytelman L,Xu Y,Lobos K B,Clare K,Walton M,Fu B,Maghirang R,Li Z,Xing Y.Development and mapping of 2240 new SSR markers for rice(Oryza sativa L.)(supplement)[J].DNA Research,2002,9(6):199-207
[9]Edwards J D,Janda J,Sweeney M T,Gaikwad A B,Liu B,Leung H,Galbraith D W.Development and evaluation of a highthroughput,low-cost genotyping platform based on oligonucleotide microarrays in rice[J].Plant Methods,2008,4(1):13
[10]Mccouch S R,Kochert G,Yu Z H,Wang Z Y,Khush G S,Coffman W R,Tanksley S D.Molecular mapping of rice chromosomes[J].Theoretical and Applied Genetics,1988,76(6):815-829
[11]Sanguinetti C J,Dias N E,Simpson A J.RAPD silver staining and recovery of PCR products separated on polyacrylamide gels[J].Biotechniques,1994,17(5):914
[12]祁鲁,刘晓,陈佳颖,林冬枝,董彦君,徐建龙.一个新的水稻温敏感叶色突变体基因定位分析[J].核农学报,2010,24(2):220-224
[13]刘钰龙,刘峰,周坤能,苏晓妹,方先文,张云辉,鲍依群.水稻温敏型叶片白化突变体tsa1的表型鉴定和基因定位[J].作物学报,2016,42(12):1754-1763
[14]邓晓娟,张海清,王悦,舒志芬,王国槐,王国梁.水稻叶色突变基因研究进展[J].杂交水稻,2012,27(5):9-30
[15]Jung K H,Hur J,Ryu C H,Choi Y,Chung Y Y,Miyao A,Hirochika H,An G.Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system[J].Plant&Cell Physiology,2003,44(5):463-472
[16]Wang P,Gao J,Wan C,Zhang F,Xu Z,Huang X,Sun X,Deng X.Divinyl chlorophyll(ide)a can be converted to monovinyl chlorophyll(ide)a by a divinyl reductase in rice[J].Plant Physiology,2010,153(3):994-1003
[17]Gothandam K M,Kim E S,Cho H,Chung Y Y.Os PPR1,a pentatricopeptide repeat protein of rice is essential for the chloroplast biogenesis[J].Plant Molecular Biology,2005,58(3):421-433
[18]Kusumi K,Yara A,Mitsui N,Tozawa Y,Iba K.Characterization of a rice nuclear-encoded plastid RNA polymerase gene OsRpo Tp[J].Plant&Cell Physiology,2004,45(9):1194-1201
[19]Hu F,Kang Z,Qiu S,Wang Y,Qin F,Yue C,Huang J,Wang G.Overexpression of Os TLP27 in rice improves chloroplast function and photochemical efficiency[J].Plant Science,2012,195(3):125-134
[20]Wang F,Wang G,Li X,Huang J,Zheng J.Heredity,physiology and mapping of a chlorophyll content gene of rice(Oryza sativa L.)[J].Journal of Plant Physiology,2008,165(3):324-330
[21]Huang J,Qin F,Zang G,Kang Z,Zou H,Hu F,Yue C,Li X,Wang G.Mutation of Os DET1 increases chlorophyll content in rice[J].Plant Science,2013,210:241-249
[22]张力科,李志彬,刘海燕,李如海,陈满元,陈爱国,钱益亮,华泽田,高用明,朱苓华.两个新的水稻叶色突变体形态结构与遗传定位研究[J].中国农业科学,2010,43(2):223-229
[23]Kong Z,Xue Y.A novel nuclear-localized CCCH-Type zinc finger protein,Os DOS,is involved in delaying leaf senescence in rice[J].Plant Physiology,2006,141(4):1376-1388
[24]Kusaba M,Ito H,Morita R,Iida S,Sato Y,Fujimoto M,Kawasaki S,Tanaka R,Hirochika H,Nishimura M.Rice non-yellow coloring1 is involved in light-harvesting complex II and grana degradation during leaf senescence[J].Plant Cell,2007,19(4):1362-1375
[25]Sato Y,Morita R,Katsuma S,Nishimura M,Tanaka A,Kusaba M.Two short-chain dehydrogenase/reductases,non-yellow coloring1 and nyc1-like,are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice[J].Plant Journal,2009,57(1):120-131
[26]李红昌,钱前,王斌,李晓波,朱立煌,徐吉臣.水稻白穗突变体基因的鉴定和染色体定位[J].科学通报,2003,48(3):268-270
[27]陈佳颖,赵剑,刘晓,李超,林冬枝,董彦君,叶胜海,张小明.一个新水稻温敏感叶色突变体的遗传分析及其基因分子定位[J].植物学报,2010,45(4):419-425
[28]Dong H,Fei G L,Wu C Y,Wu F Q,Sun Y Y,Chen M J,Ren YL,Zhou K N,Cheng Z J,Wang J L.A rice virescent-yellow leaf mutant reveals new insights into the role and assembly of plastid caseinolytic protease in higher plants[J].Plant Physiology,2013,162(4):1867-1880
[29]程欣,任德勇,马娇,朱晓燕,桑贤春,凌英华,赵芳明,何光华.水稻叶片灰白转黄突变体pyr1的鉴定与基因定位[J].作物学报,2013,39(6):992-998
[30]李广贤,姚方印,侯恒军,孙召文,姜明松,朱文银,周学标.水稻黄绿叶突变体ygl209的遗传分析与目标基因精细定位[J].作物学报,2015,41(10):1603-1611
[31]胡智,何蔷薇,郭旻,李荣德,朱茜,刘敏,严长杰.1个新水稻黄叶突变体yl1的遗传分析和初步定位[J].扬州大学学报(农业与生命科学版),2016,37(2):51-55