香菇多糖对马铃薯疮痂病抗性及生理特性的影响
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摘要
【目的】研究香菇多糖对马铃薯疮痂病抗性及生理特性的影响,为广西马铃薯疮痂病生物防治提供科学依据。【方法】在马铃薯块茎形成初期,利用不同浓度香菇多糖稀释液进行叶面喷施,测定马铃薯农艺性状和生理指,评价香菇多糖稀释液对马铃薯疮痂病抗性。【结果】不同浓度香菇多糖稀释液均显著降低马铃薯疮痂病发病率,其中800倍的香菇多糖稀释液对疮痂病防治效果最好,相对防效为79. 98%。与对照处理相比,香菇多糖处理后马铃薯叶片脯氨酸、可溶性糖、可溶性蛋白和叶绿素含量,以及POD、PAL和PPO酶活性均有所提高,MDA的含量降低,植株主茎数增加,产量和块茎营养品质均不同程度提高。【结论】香菇多糖可以作为一种植物生长调节剂和诱抗剂应用于农业生产,有利于马铃薯的生长发育,降低疮痂病发病率,提升马铃薯产量和品质。
【Objective】To provide a scientific reference for the biological control of potato scab in Guangxi,the resistance and physiological characteristics of lentinan to potato scab were studied.【Method】In the early formation of potato tubers,different concentration of gradient lentinan diluents were used to study the effects on scab resistance and physiological characters of potato.【Results】Lentinan diluents of different concentrations significantly reduced the incidence of potato scab,lentinan diluents of 800 times had the best relative control efficiency on potato scab which was 79. 98 %. Compared with the control treatment,the chlorophyll,soluble sugar,soluble protein and proline content as well as POD,PAL and PPO activity were improved after Lentinan diluents treatments,the MDA content in potato leaf was reduced,plant main stem number increased,and the yield,quality and nutrition of potato were improved.【Conclusion】Lentinan could be used as a plant growth regulator and inducer in agricultural production,which could reduce the infection rate of potato scab,promote the growth and development of potato and improve its yield and quality.
【Objective】To provide a scientific reference for the biological control of potato scab in Guangxi,the resistance and physiological characteristics of lentinan to potato scab were studied.【Method】In the early formation of potato tubers,different concentration of gradient lentinan diluents were used to study the effects on scab resistance and physiological characters of potato.【Results】Lentinan diluents of different concentrations significantly reduced the incidence of potato scab,lentinan diluents of 800 times had the best relative control efficiency on potato scab which was 79. 98 %. Compared with the control treatment,the chlorophyll,soluble sugar,soluble protein and proline content as well as POD,PAL and PPO activity were improved after Lentinan diluents treatments,the MDA content in potato leaf was reduced,plant main stem number increased,and the yield,quality and nutrition of potato were improved.【Conclusion】Lentinan could be used as a plant growth regulator and inducer in agricultural production,which could reduce the infection rate of potato scab,promote the growth and development of potato and improve its yield and quality.
引文
[1]王针针,沈艳芳,陈家吉.中国马铃薯疮痂病研究进展[A].屈冬玉,陈伊里主编.马铃薯产业与精准扶贫2017[C].哈尔滨:哈尔滨地图出版社,2017:204-210.
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[3]郝智勇.马铃薯种薯疮痂病成因及防治措施[J].黑龙江农业科学,2017(1):158-159.
[4]王杰,王开运.香菇多糖对植物真菌和病毒病害的抗病机理[J].农药,2011,50(1):16-19.
[5]吕国英,范雷法,张作法,等.香菇多糖研究进展[J].浙江农业学报,2009,21(2):183-188.
[6]彭昌家,白体坤,丁攀,等.生物农药防治棚室秋番茄病毒病效果探讨[J].中国农学通报,2016,32(7):104-108.
[7]单宏英,沈小英,陈德鑫,等.香菇多糖对烟草灰霉病的防治效果研究[J].中国烟草学报,2012,18(4):56.
[8]赵丽红.香菇多糖0. 5%水剂防治番茄病毒病田间药效试验[J].农业与技术,2017,10(37):23.
[9]姜莉莉,张中霄,王红艳,等.防治小麦纹枯病和根腐病高效药剂的筛选及其与香菇多糖联用的防效评价[J].麦类作物学报,2018,38(1):113-118.
[10]王猛,余中红,华茉莉,等. 2%香菇多糖水剂防治水稻黑条矮缩病试验[J].湖北植保,2015(1):10-11.
[11]李界秋,李杨秀,吴凡,等.三种诱抗剂诱导火龙果抗溃疡病的试验初报[J].中国南方果树,2017,46(5):54.
[12]张宪政.作物生理研究法[M].北京:农业出版社,1992:150-152.
[13]孔祥生,易现峰.植物生理学实验技术[M].北京:中国农业出版社,2008:160-162
[14]南京农业大学主编.土壤农化分析[M].北京:农业出版社,1986:388.
[15]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:197-199,246-248.
[16]王宏虬,缪福俊,李彪,等.马铃薯疮痂病最适诱抗剂筛选试验[J].南方农业学报,44(7):1125-1129.
[17]刘玲玲,李军,李长辉,等.马铃薯可溶性蛋白、叶绿素及ATP含量变化与品质抗旱性关系的研究[J].中国马铃薯,2004,18(4):201-204.
[18]苏云松,郭华春,陈伊里.马铃薯叶片SPAD值与叶绿素含量及产量的相关性[J].西南农业学报,2007,20(4):690-693.
[19]郭丽,贾秀领,张凤路,等.定位水氮组合对冀5265小麦叶片硝酸还原酶、可溶性蛋白和产量的影响[J].华北农学报,2010,25(1):180-184.
[20]余斌,黄锐,南运有,等.马铃薯悬浮细胞对温度胁迫的生理响应机制[J].甘肃农业科技,2017(7):15-19.
[21]Babita M,Maheswari M,LM Rao,et al. Osmotic adjustment,drought tolerance and yield in castor(Ricinus communis L.)hybrids[J]. Environmental and Experimental Botany,2010(6):243-249.
[22]李鹏鹏,王杰,乔康,等.香菇多糖浸种对黄瓜幼苗抗霜霉病及生理特性的影响[J].农药,2014,53(4):287-295.
[23]刘星海.植物多糖复合制剂对作物生长及产量的调控作用和机理研究[D].中国农业科学院研究生院硕士学位论文,2011:28-32.
[24]陈顺,席海生,冯华,等.香菇多糖防治由病毒引起马铃薯退化的试验研究[J].微生物学杂志,1998:18(2):40-41.
[25]刘艳芝,马井玉,徐祥文,等.香菇多糖水剂防治番茄病毒病田间药效试验[J].蔬菜,2014(6):6-8.
[26]邓劲松,孙光忠,袁浩. 0. 5%香菇多糖水剂防治番茄病毒病效果[J].湖北植保,2015(5):11-58.
[27]铃木直治,张际中.近代植物病理化学[M].上海:上海科学技术出版社,1985.
[28]贾显禄,王振中,王平.水稻与稻瘟病菌非亲和性互作中重要防御酶活性变化规律研究[J].植物病理学报,2002,32(3):206-213.
[29]余叔文.植物生理与分子生物学[M].北京:科学出版社,1992:417-430.
[30]张笑宇,于肖夏,高翔,等.马铃薯黑痣病毒素诱导马铃薯幼苗丙二醛含量、细胞膜透性及PAL活性的变化[J].内蒙古农业大学学报,2012,33(2):16-20.
[31]罗琰.香菇多糖对烟草花叶病毒的抑制作用研究[J].现代农业科技,2013(24):147-148.
[32]张文华.香菇多糖增强黄瓜幼苗对炭疽病菌抗性机理[J].生物技术通报,2016,32(2):93-97.
[2]王腾,马爽,孙继英,等.不同马铃薯品种对疮痂病的田间抗性比较[J].黑龙江农业科学,2018(2):58-59.
[3]郝智勇.马铃薯种薯疮痂病成因及防治措施[J].黑龙江农业科学,2017(1):158-159.
[4]王杰,王开运.香菇多糖对植物真菌和病毒病害的抗病机理[J].农药,2011,50(1):16-19.
[5]吕国英,范雷法,张作法,等.香菇多糖研究进展[J].浙江农业学报,2009,21(2):183-188.
[6]彭昌家,白体坤,丁攀,等.生物农药防治棚室秋番茄病毒病效果探讨[J].中国农学通报,2016,32(7):104-108.
[7]单宏英,沈小英,陈德鑫,等.香菇多糖对烟草灰霉病的防治效果研究[J].中国烟草学报,2012,18(4):56.
[8]赵丽红.香菇多糖0. 5%水剂防治番茄病毒病田间药效试验[J].农业与技术,2017,10(37):23.
[9]姜莉莉,张中霄,王红艳,等.防治小麦纹枯病和根腐病高效药剂的筛选及其与香菇多糖联用的防效评价[J].麦类作物学报,2018,38(1):113-118.
[10]王猛,余中红,华茉莉,等. 2%香菇多糖水剂防治水稻黑条矮缩病试验[J].湖北植保,2015(1):10-11.
[11]李界秋,李杨秀,吴凡,等.三种诱抗剂诱导火龙果抗溃疡病的试验初报[J].中国南方果树,2017,46(5):54.
[12]张宪政.作物生理研究法[M].北京:农业出版社,1992:150-152.
[13]孔祥生,易现峰.植物生理学实验技术[M].北京:中国农业出版社,2008:160-162
[14]南京农业大学主编.土壤农化分析[M].北京:农业出版社,1986:388.
[15]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:197-199,246-248.
[16]王宏虬,缪福俊,李彪,等.马铃薯疮痂病最适诱抗剂筛选试验[J].南方农业学报,44(7):1125-1129.
[17]刘玲玲,李军,李长辉,等.马铃薯可溶性蛋白、叶绿素及ATP含量变化与品质抗旱性关系的研究[J].中国马铃薯,2004,18(4):201-204.
[18]苏云松,郭华春,陈伊里.马铃薯叶片SPAD值与叶绿素含量及产量的相关性[J].西南农业学报,2007,20(4):690-693.
[19]郭丽,贾秀领,张凤路,等.定位水氮组合对冀5265小麦叶片硝酸还原酶、可溶性蛋白和产量的影响[J].华北农学报,2010,25(1):180-184.
[20]余斌,黄锐,南运有,等.马铃薯悬浮细胞对温度胁迫的生理响应机制[J].甘肃农业科技,2017(7):15-19.
[21]Babita M,Maheswari M,LM Rao,et al. Osmotic adjustment,drought tolerance and yield in castor(Ricinus communis L.)hybrids[J]. Environmental and Experimental Botany,2010(6):243-249.
[22]李鹏鹏,王杰,乔康,等.香菇多糖浸种对黄瓜幼苗抗霜霉病及生理特性的影响[J].农药,2014,53(4):287-295.
[23]刘星海.植物多糖复合制剂对作物生长及产量的调控作用和机理研究[D].中国农业科学院研究生院硕士学位论文,2011:28-32.
[24]陈顺,席海生,冯华,等.香菇多糖防治由病毒引起马铃薯退化的试验研究[J].微生物学杂志,1998:18(2):40-41.
[25]刘艳芝,马井玉,徐祥文,等.香菇多糖水剂防治番茄病毒病田间药效试验[J].蔬菜,2014(6):6-8.
[26]邓劲松,孙光忠,袁浩. 0. 5%香菇多糖水剂防治番茄病毒病效果[J].湖北植保,2015(5):11-58.
[27]铃木直治,张际中.近代植物病理化学[M].上海:上海科学技术出版社,1985.
[28]贾显禄,王振中,王平.水稻与稻瘟病菌非亲和性互作中重要防御酶活性变化规律研究[J].植物病理学报,2002,32(3):206-213.
[29]余叔文.植物生理与分子生物学[M].北京:科学出版社,1992:417-430.
[30]张笑宇,于肖夏,高翔,等.马铃薯黑痣病毒素诱导马铃薯幼苗丙二醛含量、细胞膜透性及PAL活性的变化[J].内蒙古农业大学学报,2012,33(2):16-20.
[31]罗琰.香菇多糖对烟草花叶病毒的抑制作用研究[J].现代农业科技,2013(24):147-148.
[32]张文华.香菇多糖增强黄瓜幼苗对炭疽病菌抗性机理[J].生物技术通报,2016,32(2):93-97.