MicroRNA-590-5p、microRNA-103、microRNA-34c-5p、Dicer及BRMS-1基因在肺癌中的表达研究
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摘要
目的探讨microRNA-590-5p(miR-590-5p)、miR-103、miR-34c-5p、Dicer及BRMS-1在肺癌中表达及相互间关系。方法选取47例肺癌患者的癌组织及癌旁正常组织,利用Trizol提取总RNA,实时荧光定量聚合酶链反应检测基因的相对表达量。结果肺癌组织与癌旁正常组织miR-103、miR-34c-5p、Dicer及BRMS-1基因表达水平比较,差异有统计学意义(P<0.05),miR-34c-5p与P53呈正相关(P<0.05),miR-103与多药耐药相关蛋白呈正相关(P<0.05),Dicer与miR-34c-5p、miR-103及miR-590-5p呈负相关(P<0.05)。结论在肺癌组织中Dicer基因表达与miR-34c-5p呈负相关,可能通过抑制癌基因的表达,抑制肺癌的发生。
Objective To investigate the miR-590-5 p, miR-103, miR-34 c-5 p, and Dicer and BRMS1 gene expressions in lung cancer and to explore relationships among them. Methods The lung cancer tissues and the paracancerous tissues of 47 patients were collected. The total RNA was extracted by TRIzol. q RT-PCR was used to detect the relative expressions of the genes. Results There were significant differences in the expressions of miR-103, miR-34 c-5 p, and Dicer and BRMS1 genes between the lung cancer tissues and the adjacent tissues(P < 0.05). The expressions of miR-34 c-5 p and P53 was positively correlated(P < 0.05). The expression of miR-103 was positively correlated to multidrug resistance-associated protein(P < 0.05). Dicer was negatively correlated with miR-34 c-5 p, miR-103 and miR-590-5 p(P < 0.05). Conclusions Dicer gene expression is negatively associated with the expression of miR-34 c-5 p in lung cancer tissues. The expression of miR-34 c-5 p may inhibit the occurrence and development of lung cancers through inhibition of oncogenes.
Objective To investigate the miR-590-5 p, miR-103, miR-34 c-5 p, and Dicer and BRMS1 gene expressions in lung cancer and to explore relationships among them. Methods The lung cancer tissues and the paracancerous tissues of 47 patients were collected. The total RNA was extracted by TRIzol. q RT-PCR was used to detect the relative expressions of the genes. Results There were significant differences in the expressions of miR-103, miR-34 c-5 p, and Dicer and BRMS1 genes between the lung cancer tissues and the adjacent tissues(P < 0.05). The expressions of miR-34 c-5 p and P53 was positively correlated(P < 0.05). The expression of miR-103 was positively correlated to multidrug resistance-associated protein(P < 0.05). Dicer was negatively correlated with miR-34 c-5 p, miR-103 and miR-590-5 p(P < 0.05). Conclusions Dicer gene expression is negatively associated with the expression of miR-34 c-5 p in lung cancer tissues. The expression of miR-34 c-5 p may inhibit the occurrence and development of lung cancers through inhibition of oncogenes.
引文
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[3]SAITO M,SCHETTER A J,MOLLERUP S,et al.The association of mircro RNA expression with prognosis and progression in the early-stage,non-small cell lung adknocarcinoma:a retrospective analysis of three cohorts[J].Clin Cancer Res,2011,17(7):1875-1882.
[4]MALLETER M,JACQUOT C,RONSSEAU B,et al.mi RNAs,a potential target the treatment of non-small-cell lung carcinomas[J].Gene,2012,506(2):355-359.
[5]LORIO M V,CROCE C M.Micro RNAs in cancer:small molecules with a huge impact[J].Clin Oncol,2009,27(34):5848-5856.
[6]LOPEZ-SERRA P,ESTELLER M.DNA methylation-associated silencing of tumor-suppressor micro RNAs in cancer[J].Oncogene,2012,31(13):1609-1622.
[7]NEGRINI M,CALIN G A.Breast cancer metastasis:a micro RNA story[J].Breast Cancer Res,2008,10(2):203.
[8]WANG X,TANG S,LE S Y,et al.Aberrant expression of oncogenic and tumor-suppressive micro RNAs in cervical cancer is required for cancer cell growth[J].PLo S One,2008,3(7):DOI:10.1371/journal.pone.0002557.
[9]WANG X C,TIAN L L,JIANG X Y,et al.The expression and function of mi RNA-451 in non-small cell lung cancer[J].Cancer Lett,2011,311(2):203-209.
[10]ZHENG Z M,WANG X.Regulation of cellular mi RNA expression by human papillomaviruses[J].Biochim Biophys Acta,2011,1809(11-12):668-677.
[11]CHEN H Y,LIN Y M,CHUNG H C,et al.mi R-103/107 promote metastasis of colorectal cancer by targeting the metastasis suppressors DAPK and KLF4[J].Cancer Res,2012,72(14):3631-3641.
[12]GENG L,SUN B,GAO B,et al.Micro RNA-103 promotes colorectal cancer by targeting tumor suppressor DICER and PTEN[J].International Journal of Molecular Sciences,2014,15(1):8458-8472.
[13]FABER C,HORST D,HLUBEK F,et al.Overexpression of Dicer predicts poor survival in colorectal cancer[J].Eur J Canc(Oxford,England:1990),2011,47(9):1414-1419.
[14]JAKYMIW A,PATEL R S,DEMING N,et al.Overexpression of dicer as a result of reduced let-7 Micro RNA levels contributes to increased cell proliferation of oral cancer cells[J].Genes Chromosome Canc,2010,49(6):549-559.
[15]CHIOSEA S,JELEZCOVA E,CHANDRAN U,et al.Upregulation of dicer,a component of the Micro RNA machinery,in prostate adenocarcinoma[J].Am J Pathol,2006,169(5):1812-1820.
[16]LILIANA P CANTINI,LOURDES M ANDINO,CHRISTOPHER C ATTAWAY,et al.Identification and characterization of Dicer1e,a Dicer1 protein variant,in oral cancer cells[J].Molecular Cancer2014,13(1):190-205.
[17]BALGKOURANIDOU I,CHIMONIDOU M,MILAKI G,et al.Breast cancer metastasis suppressor-1 promoter methyla tion in cell-free DNA provides prognostic infor mation in non-small cell lung cancer[J].Br J Cancer,2014,110(8):2054-2062.
[18]CUI R X,LIU N,HE Q M,et al.Low BRMS1 ex pression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associat ed with poor patient survival[J].BMC Cancer,2012,12:376.
[19]曾龙剑,吴锡南,杨凯云,等.mi R-10b、mi R-18b及mi R-130b在肺癌中的表达研究[J].中国现代医学杂志,2014,24(10):28-31.