酪氨酸激酶2与P53在乳腺癌中表达的相关性及与临床病理特征之间的关系
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摘要
目的探究酪氨酸激酶2(TYK2)与P53在乳腺癌(MC)中表达的相关性及与临床病理特征之间的关系。方法回顾性选择2014年11月至2017年11月眉山市人民医院收治并确诊的87例乳腺癌患者的癌组织及癌旁正常乳腺组织,另收集42例乳腺纤维腺瘤患者的腺瘤组织。采用免疫组化法检测TYK2、P53在MC组织、乳腺纤维腺瘤组织及癌旁正常乳腺组织中表达水平;分析TYK2、P53在乳腺癌中表达的相关性;比较TYK2、P53表达水平与各临床病理特征的关系。结果 TYK2在MC组织、乳腺纤维腺瘤组织、癌旁正常乳腺组织中阳性表达率分别为57. 47%(50/87)、6. 90%(6/87)、2. 38%(1/42),P53在MC组织、乳腺纤维腺瘤组织、癌旁正常乳腺组织中阳性表达率分别为40. 23%(35/87)、3. 45%(3/87)、2. 38%(1/42),TYK2和P53在MC组织中阳性表达率明显高于乳腺纤维腺瘤组织、癌旁正常乳腺组织,差异均具有统计学意义(P <0. 05);三阴性乳腺癌中TYK2阳性表达率略高于HER-2过表达型、Luminal B型及Luminal A型乳腺癌,差异无统计学意义(P> 0. 05);三阴性乳腺癌中P53阳性表达率显著高于HER-2过表达型、Luminal B型及Luminal A型乳腺癌,差异具有统计学意义(P <0. 05);肿瘤直径、临床分期不同的MC患者癌组织中TYK2表达水平差异具有统计学意义(P <0. 05);淋巴结转移不同的MC患者癌组织中P53表达水平差异具有统计学意义(P <0. 05); TYK2与P53在乳腺癌中的表达呈正相关(P <0. 05)。结论 TYK2与P53在乳腺癌中呈高表达趋势;且TYK2的表达水平与肿瘤直径、临床分期有关; P53的表达水平与乳腺癌分子分型、淋巴结转移有关; TYK2与P53在乳腺癌中的表达呈正相关。
Objective To investigate the correlation between the expressions of tyrosine kinase 2( TYK2) and P53 in mammary cancer( MC) and its relationship with clinicopathological features. Methods In this study,the expressions of TYK2 and P53 in MC tissues,fibroadenoma tissues and adjacent breast tissues collected from MC patients( n = 87) and breast fibroadenoma patients( n = 42) treated in our hospital from November 2014 To November 2017 were detected by immunohistochemistry,then its correlation with clinicopathological features was analyzed. Results The positive expression rates of TYK2 in MC tissues,breast fibroadenoma tissues,and adjacent normal tissues were 57. 47%( 50/87),6.90%( 6/87),and 2. 38%( 1/42),respectively,while these were 40. 23%( 35/87),3. 45%( 3/87),and 2. 38%( 1/42) for P53,with statistic difference( P < 0. 05). The positive expression rate of TYK2 in triple negative breast cancer was slightly higher than that in HER-2 overexpression subtype,Luminal B subtype and Luminal A subtype,and the difference was not statistically significant( P > 0. 05). The positive expression rate of P53 in triple negative breast cancer was slightly higher than that in HER-2 overexpression subtype,Luminal B subtype and Luminal A subtype,with statistic difference( P < 0. 05). The expression of TYK2 in cancer tissues with different tumor diameter and clinical stage was statistically significant( P < 0. 05). The expression of P53 in MC tissues with different lymph node metastasis was statistically significant( P< 0. 05). The expression of TYK2 and P53 in MC tissues was positively correlated( P < 0. 05). Conclusion TYK2 and P53 are highly expressed in MC tissues,and the expression level of TYK2 is related to tumor diameter and clinical stage,while the expression level of P53 is related to molecular typing and lymph node metastasis. Additionally,the expression levels of TYK2 and P53 are positively correlated.
Objective To investigate the correlation between the expressions of tyrosine kinase 2( TYK2) and P53 in mammary cancer( MC) and its relationship with clinicopathological features. Methods In this study,the expressions of TYK2 and P53 in MC tissues,fibroadenoma tissues and adjacent breast tissues collected from MC patients( n = 87) and breast fibroadenoma patients( n = 42) treated in our hospital from November 2014 To November 2017 were detected by immunohistochemistry,then its correlation with clinicopathological features was analyzed. Results The positive expression rates of TYK2 in MC tissues,breast fibroadenoma tissues,and adjacent normal tissues were 57. 47%( 50/87),6.90%( 6/87),and 2. 38%( 1/42),respectively,while these were 40. 23%( 35/87),3. 45%( 3/87),and 2. 38%( 1/42) for P53,with statistic difference( P < 0. 05). The positive expression rate of TYK2 in triple negative breast cancer was slightly higher than that in HER-2 overexpression subtype,Luminal B subtype and Luminal A subtype,and the difference was not statistically significant( P > 0. 05). The positive expression rate of P53 in triple negative breast cancer was slightly higher than that in HER-2 overexpression subtype,Luminal B subtype and Luminal A subtype,with statistic difference( P < 0. 05). The expression of TYK2 in cancer tissues with different tumor diameter and clinical stage was statistically significant( P < 0. 05). The expression of P53 in MC tissues with different lymph node metastasis was statistically significant( P< 0. 05). The expression of TYK2 and P53 in MC tissues was positively correlated( P < 0. 05). Conclusion TYK2 and P53 are highly expressed in MC tissues,and the expression level of TYK2 is related to tumor diameter and clinical stage,while the expression level of P53 is related to molecular typing and lymph node metastasis. Additionally,the expression levels of TYK2 and P53 are positively correlated.
引文
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[12]邓亚平,钟晶敏,李晶,等. Fas相关死亡结构域蛋白在乳腺癌中表达的临床病理研究[J].现代生物医学进展,2016,16(26):5068-5071.
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[14] Zhao G,Zhu G,Huang Y,et al. IL-6 mediates the signal pathway of JAK-STAT3-VEGF-C promoting growth,invasion and lymphangiogenesis in gastric cancer[J]. Oncol Rep,2016,35(3):1787.
[15]张绪良,黄丹青,周俊伟. Survivin蛋白及P53在乳腺癌中的表达及临床意义[J].临床和实验医学杂志,2015,14(17):1446-1448.
[16] Molina-Vila MA,Bertran-Alamillo J,GascóA,et al. Nondisruptive p53 mutations are associated with shorter survival in patients with advanced non-small cell lung cancer[J]. Clin Cancer Res,2014,20(17):4647-4659.
[17] Lee JY,Kim HJ,Yoon NA,et al. Tumor suppressor p53 plays a key role in induction of both tristetraprolin and let-7 in human cancer cells[J]. Nucleic Acids Res,2013,41(11):5614-5625.
[2] Odero-Marah VA,Khalkhali-Ellis Z,Schneider GB,et al. Tyrosine phosphorylation of maspin in normal mammary epithelia and breast cancer cells[J]. Biochem Biophys Res Commun,2016,295(4):800-805.
[3] Lucie C,Goupille C,Charly B,et al. Long chain n-3 polyunsaturated fatty acids increase the efficacy of docetaxel in mammary cancer cells by downregulating Akt and PKCε/δ-induced ERK pathways[J]. BBAMolecular and Cell Biology of Lipids,2016,1861(4):380-390.
[4] Liu TY,Zhang H,Du SM,et al. Expression of microRNA-210 in tissue and serum of renal carcinoma patients and its effect on renal carcinoma cell proliferation,apoptosis,and invasion[J]. Genet Mol Rese,2016,15(1):1501776.
[5]吕秀芳,宋晓霞,贺慧,等. p16、p53及hTERC在人乳头状瘤病毒检测阴性子宫颈鳞状细胞癌中的表达[J].中华病理学杂志,2016,45(11):791-792.
[6]艾晓晴,贾喜花,王晓博,等. UHRF1和P53在结肠癌中的表达及临床意义[J].国际肿瘤学杂志,2016,43(5):346-349.
[7]崔衢,刘静,张静,等. Tyk2促进前列腺癌细胞系雄激素受体特异位点Tyr-267磷酸化[J].基础医学与临床,2016,36(11):1499-1504.
[8]李狄航,梁运升.乳腺癌分子分型及其临床治疗意义[J].中国基层医药,2018,25(17):2309-2312.
[9]郝磊,魏现娟,郝坤. p53及bcl-2蛋白在乳腺癌中表达的相关性研究[J].中国妇幼保健,2017,32(4):695-697.
[10]程凯,余波,周晓蝶,等. GATA3在乳腺癌中表达的敏感性和病理诊断的价值[J].诊断病理学杂志,2016,23(5):374-377.
[11]庄晓苹,谭晓,朱暐,等. SyK和HGF在乳腺癌中表达及临床意义[J].医学研究杂志,2016,45(11):145-148.
[12]邓亚平,钟晶敏,李晶,等. Fas相关死亡结构域蛋白在乳腺癌中表达的临床病理研究[J].现代生物医学进展,2016,16(26):5068-5071.
[13] Khanna A,Sharma MK. Selfie use:The implications for psychopathology expression of body dysmorphic disorder[J]. Ind Psychiatry J,2017,26(1):106-109.
[14] Zhao G,Zhu G,Huang Y,et al. IL-6 mediates the signal pathway of JAK-STAT3-VEGF-C promoting growth,invasion and lymphangiogenesis in gastric cancer[J]. Oncol Rep,2016,35(3):1787.
[15]张绪良,黄丹青,周俊伟. Survivin蛋白及P53在乳腺癌中的表达及临床意义[J].临床和实验医学杂志,2015,14(17):1446-1448.
[16] Molina-Vila MA,Bertran-Alamillo J,GascóA,et al. Nondisruptive p53 mutations are associated with shorter survival in patients with advanced non-small cell lung cancer[J]. Clin Cancer Res,2014,20(17):4647-4659.
[17] Lee JY,Kim HJ,Yoon NA,et al. Tumor suppressor p53 plays a key role in induction of both tristetraprolin and let-7 in human cancer cells[J]. Nucleic Acids Res,2013,41(11):5614-5625.