弓背青鳉3种雌激素受体基因的克隆及其表达分析
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摘要
【目的】克隆弓背青鳉(Oryzias curvinotus)雌激素受体基因(Estrogen receptor, er),分析在组织、胚胎发育时期中表达特征和雌二醇暴露后表达响应,为探究er介导雌激素调控机制提供理论依据。【方法】通过弓背青鳉肝脏转录组数据筛选进行er序列扩增,实时定量PCR(RT-q PCR)检测不同组织、不同胚胎发育时期和雌二醇暴露后的er表达量。【结果】3种ocuerα/erβ1/erβ2的编码区序列分别为1 860 bp、1 689 bp和2 007 bp,编码氨基酸个数相应为619、562和688。定量结果显示,3种er主要在肝脏和性腺高表达。胚胎发育过程中,ocuerα在卵裂期至囊胚期表达量低,在囊胚期后表达量逐渐上升持续到孵化;2种ocuerβ亚型均在胚胎发育初期有较高表达,随后ocuerβ1在原肠胚后开始降低,ocuerβ2在囊胚期后开始降低,在器官发育后期表达量均逐渐上升直至孵化。弓背青鳉仔鱼雌二醇暴露结果显示,各浓度雌二醇暴露均能使ocuerα和弓背青鳉卵黄蛋白基因(Vitellogenin,vtg)表达量上升,ocuerβ1仅在20μg/L上调,ocuerβ2表达量下调。【结论】ocuer不同亚型在介导雌激素调控中起着重要作用。
【Objective】To provide a theoretical basis for the mechanisms of estrogen receptor functions of Oryzias curvinotus, ocuer genes were cloned and analyzed. The tissue distribution and expression of embryonic development stage and response to estradiol were also evaluated. 【Method】The ocuer cDNA was amplified by the combination of transcriptome and reverse transcription PCR(RT-PCR).Tissue distribution of ocuer genes and expression of embryonic development stage and response to estradiol were analyzed by RT-qPCR. 【Result】The lengths of ocuerα/erβ1/erβ2 are 1971 bp, 2006 bp and 2365 bp, respectively, encoding of 619, 562 and 688 amino acids, correspondingly. The result of RT-qPCR showed that three ers were mainly expressed in the liver and gonads. The ocuerα was highly expressed in testis, ovary and female liver; ocuerβ1 was highly expressed in ovary and male liver;ocuerβ2 was highly expressed in gonad and liver. During the development of embryonic, the level of ocuerα expression was low, then increased gradually until hatching after blastocyst stage; both ocuerβsubtypes were highly expressed in the early stage of embryonic development, then decreased, but increased in the last stage of organ development until hatching. The exposure of E2 showed that the expression of ocuerα and ocuvtg increased at each concentration of E2 in the larvae of Oryzias curvinotu,and ocuerβ1 was only up-regulated at 20 μg/L but the ocuerβ2 was down-regulated. 【Conclusion】The ocuer gene play an important role in estrogen regulation.
【Objective】To provide a theoretical basis for the mechanisms of estrogen receptor functions of Oryzias curvinotus, ocuer genes were cloned and analyzed. The tissue distribution and expression of embryonic development stage and response to estradiol were also evaluated. 【Method】The ocuer cDNA was amplified by the combination of transcriptome and reverse transcription PCR(RT-PCR).Tissue distribution of ocuer genes and expression of embryonic development stage and response to estradiol were analyzed by RT-qPCR. 【Result】The lengths of ocuerα/erβ1/erβ2 are 1971 bp, 2006 bp and 2365 bp, respectively, encoding of 619, 562 and 688 amino acids, correspondingly. The result of RT-qPCR showed that three ers were mainly expressed in the liver and gonads. The ocuerα was highly expressed in testis, ovary and female liver; ocuerβ1 was highly expressed in ovary and male liver;ocuerβ2 was highly expressed in gonad and liver. During the development of embryonic, the level of ocuerα expression was low, then increased gradually until hatching after blastocyst stage; both ocuerβsubtypes were highly expressed in the early stage of embryonic development, then decreased, but increased in the last stage of organ development until hatching. The exposure of E2 showed that the expression of ocuerα and ocuvtg increased at each concentration of E2 in the larvae of Oryzias curvinotu,and ocuerβ1 was only up-regulated at 20 μg/L but the ocuerβ2 was down-regulated. 【Conclusion】The ocuer gene play an important role in estrogen regulation.
引文
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[8]李红,张金鹏,廉颖,等.金钱鱼雌激素受体erα,erβ1和erβ2克隆及表达分析[J].生物学杂志,2017,34(2):40-45.
[9]HU P,MENG Z,JIA Y.Molecular characterization and quantification of estrogen receptors in turbot(Scophthalmus maximus)[J].General and Comparative Endocrinology,2018,257:38-49.
[10]CHAKRABORTY T,SHIBATA Y,ZHOU L Y,et al.Differential expression of three estrogen receptor subtype m RNAs in gonads and liver from embryos to adults of the medaka,Oryzias latipes[J].Molecular and Cellular Endocrinology,2011,333(1):47-54.
[11]FILBY A L,TYLER C R.Molecular characterization of estrogen receptors 1,2a,and 2b and their tissue and ontogenic expression profiles in fathead minnow(Pimephales promelas)[J].Biology of Reproduction,2005,73(4):648-662.
[12]KATSU Y,KOHNO S,HYODO S,et al.Molecular cloning,characterization,and evolutionary analysis of estrogen receptors from phylogenetically ancient fish[J].Endocrinology,2008,149(12):6300-6310.
[13]郑宇新,张妮娜,崔亚娟,等.雌激素受体在卵泡发育中作用的研究进展[J].黑龙江畜牧兽医,2018(1):60-62.
[14]FITZPATRICK,JENNIFER L,MIZE,et al.Estrogen-mediated neuroprotection againstβ-amyloid toxicity requires expression of estrogen receptorαorβand activation of the MAPK pathway[J].Journal of Neurochemistry,2002,82(3):674-682.
[15]EDDY E M,WASHBURN T F,BUNCH D O,et al.Targeted disruption of the estrogen receptor gene in male mice causes alteration of spermatogenesis and infertility[J].Endocrinology,1996,137(11):4796-4805.
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[17]HAYAKAWA H,LE Q D,KINOSHITA M,et al.Genetic similarity of the Hainan medaka populations collected from hyper-and hypo-osmotic environments in northern Vietnam[J].Ocean Science Journal,2015,50(2):231-235.
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[19]廖健.雷州半岛红树林区仔稚幼鱼多样性及弓背青鳉对壬基酚的响应[D].湛江:广东海洋大学,2017.
[20]Iwamatsu T.Stages of normal development in the medaka Oryzias latipes[J].Mechanisms of Development,2004,121(7/8):605-618.
[21]DONG Z,CHEN P,ZHANG N,et al.Evaluation of reference genes for quantitative real-time PCR analysis of gene expression in Hainan medaka(Oryzias curvinotus)[J].Gene Reports,2019,14:94-99.
[22]SABOATTWOOD T,KROLL K J,DENSLOW N D.Differential expression of largemouth bass(Micropterus salmoides)estrogen receptor isotypes alpha,beta,and gamma by estradiol[J].Molecular&Cellular Endocrinology,2004,218(1):107-118.
[23]NILSSON S,MAKELA S,TREUTER E,et al.Mechanisms of estrogen action[J].Physiological Reviews,2001,81(4):1535-1565.
[24]KIM K,THU N,SAVILLE B,et al.Domains of estrogen receptorα(ERα)required for erα/sp1-mediated activation of GC-rich promoters by estrogens and antiestrogens in breast cancer cells[J].Molecular Endocrinology,2003,17(5):804-817.
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[26]PETERS G A,KHAN S A.Estrogen receptor domains Eand F:role in dimerization and interaction with coactivator RIP-140[J].Molecular Endocrinology,1999,13(2):286-296.
[27]KIM J G,KIM H R,PARK Y J,et al.Molecular cloning and characterization of the estrogen receptor from the striped bitterling(Acheilognathus yamatsutae)[J].Environmental Health and Toxicology,2011,26:e2011005
[28]SOCORRO,S.Two estrogen receptors expressed in the teleost fish,Sparus aurata:c DNA cloning,characterization and tissue distribution[J].Journal of Endocrinology,2000,166(2):293-306.
[29]GAYATHRI C,AMENA A,JANAKE G,et al.Levels of17β-estradiol receptors expressed in embryonic and adult zebrafish following in vivo treatment of natural or synthetic ligands[J].Plos One,2010,5(3):e9678.
[30]MENG X,BARTHOLOMEW C,CRAFT J A.Differential expression of vitellogenin and oestrogen receptor genes in the liver of zebrafish,Danio rerio[J].Analytical and Bioanalytical Chemistry,2010,396(2):625-630.
[31]HALM S,MARTINEZRODRIGUEZ G,RODRIGUEZ L,et al.Cloning,characterisation,and expression of three oestrogen receptors(ER alpha,ER beta1 and ER beta2)in the European sea bass,Dicentrarchus labrax[J].Molecular&Cellular Endocrinology,2004,223(1):63-75.
[32]蒲鲁鲁.大黄鱼雌激素受体与雄激素受体基因的克隆与表达分析[D].厦门:集美大学,2011.
[33]管昕.唐鱼雌激素受体c DNA克隆及表达分析[D].广州:华南师范大学,2007.
[34]NIKOLERIS L,HANSSON M.Unraveling the estrogen receptor(ER)genes in Atlantic salmon(Salmo salar)reveals expression differences between the two adult life stages but little impact from polychlorinated biphenyl(PCB)load[J].Molecular&Cellular Endocrinology,2015,400(C):10-20.
[35]WANG D S,SENTHILKUMARAN B,SUDHAKUMARI C C,et al.Molecular cloning,gene expression and characterization of the third estrogen receptor of the Nile tilapia,Oreochromis niloticus[J].Fish Physiology and Biochemistry,2005,31(2-3):255-266.
[36]宋明月,单喜双,陈细华等.兴国红鲤ER基因克隆表达及EE2暴露对肝中ER表达的影响[J].水生生物学报,2017,41(1):26-32.
[37]NAGLER J J,CAVILEER T,SULLIVAN J,et al.The complete nuclear estrogen receptor family in the rainbow trout:Discovery of the novel ERα2 and both ERβisoforms[J].Gene,2007,392(1-2):164-173.
[38]王厚鹏.稀有鮈鲫ER基因的克隆及内分泌干扰物对其表达的影响[D].杨凌:西北农林科技大学,2012.
[39]YARON Z,LEVAVI-SIVAN B.Endocrine regulation of fish reproduction[J].Encyclopedia of Fish Physiology:from Genome to Environment,2011,2:1500-1508.
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